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Antosiyaninlerin Biyoteknolojik Yöntemlerle Üretimi

Year 2020, Volume: 4 Issue: 1, 1 - 9, 31.01.2020

Abstract

Amaç: Bitkiler
sağlık sektöründen gıda sektörüne, kozmetikten tekstil sektörüne kadar çok
geniş bir yelpazede kullanılan bazı sekonder bileşikleri üretmektedirler. Bu
bileşiklerden bazılarının insanlarda antioksidan, antiinflamatuar, antialerjik,
antiülser, antibiyotik ve antikanserojenik etkilere sahip olduklarının
belirlenmesi ile birlikte bu bileşikleri yoğun olarak üreten bitkilere ilgi
artmıştır. Söz konusu bileşiklerin yüksek miktarlarda elde edilmesi önemli olup,
geleneksel ekstraksiyon yöntemleri ile düşük miktar ve saflıklarda elde
edilebildikleri bilinmektedir.

Yöntem: Bu bileşiklerin biyoteknolojik yöntemlerle
daha yüksek miktar, kalite ve saflıkta elde edilebilmesi, çalışmaların bu alana
odaklanmasını sağlamıştır. Bu çalışma ile de Alphonse Lavalle üzüm çeşidine ait
yapraklardan kallus kültürü ile antosiyanin bileşiklerinin üretilmesi ve HPLC
ile belirlenmesi amaçlanmıştır.

Bulgular: Altı
farklı besin ortamının kullanıldığı bu araştırma sonucunda kalluslardan elde
edilen antosiyanin miktarlarının 8.716 ile 94.501 µg/g arasında değiştiği
belirlenmiştir.

Sonuç: İnsan sağlığı açısından son derece değerli,
aynı zamanda katma değeri de yüksek olan bileşenlerin çevreyi tahrip etmeksizin
yüksek miktarlarda ve sürdürülebilir üretimi için doku kültürü teknikleri ile
farklı uygulamalarla eldesi önemlidir.

References

  • 1. Birudu, R.B. & Naik, M.J. (2014). Anticancer properties of secondary metabolites of medicinal plants in carcinoma. British Biomedical Bulletin, 2(4), 662-668. 2. Kennedy, D.O. (2014). Polyphenols and the human brain: Plant “secondary metabolite” ecologic roles and endogenous signaling functions drive benefits. American Society for Nutrition Advances in Nutrition, 5(5), 515-533. 3. Wink, M. (2015). Modes of action of herbal medicines and plant secondary metabolites. Medicines, 2(3), 251-286. 4. Luciano, A.J., Irineo, T.P., Rosalía Virginia, O.V., Feregrino-Pérez, A.A., Hernández, A.C. & Ramón Gerardo, G.G. (2017). Integrating plant nutrients and elicitors for production of secondary metabolites, sustainable crop production and human health: A Review. International Journal of Agriculture and Biology, 19(3), 391-402. 5. Decendit, A. & Merillon, J.M. (1996). Condensed tannin and anthocyanin production in Vitis vinifera cell suspension cultures. Plant Cell Reports, 15(10), 762-765. 6. Vitrac, X., Krisa, S., Decendit, A., Vercauteren, J., Nührich, A., Monti, J.P. et al. (2002). Carbon-14 biolabelling of wine polyphenols in Vitis vinifera cell suspension cultures. Journal of Biotechnology, 95(1), 49-56. 7. Waterhouse, A.L. (1995). Wine and heart disease. Chemical Industry, 9, 338-341. 8. Merillon, J.M., Fauconneau, B., Waffo Teguo, P., Barrier, L., Vercauteren, J. & Huguet, F. (1997). Antioxidant activity of the stilbene astringin, newly extracted from Vitis vinifera cell cultures. Clinical Chemistry, 43(6), 1092-1093. 9. Jennings, A., Welch, A.A., Spector, T., Macgregor, A. & Cassidy, A. (2014). Intakes of anthocyanins and flavones are associated with biomarkers of insulin resistance and inflammation in women. Journal of Nutrition, 144(2), 202-208. 10. Khoo, H.E., Azlan, A., Tang, S.T. & Lim, S.M. (2017). Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Journal of Food and Nutrition Research, 61(1), 1-21. 11. Juranić, Z. & Zizak, Z. (2005). Biological activities of berries: from antioxidant capacity to anti-cancer effects. Biofactors, 23(4), 207-211. 12. He, K, Li, X. & Chen, X. (2011). Evaluation of antidiabetic potential of selected traditional Chinese medicines in STZ-induced diabetic mice. Journal of Ethnopharmacology, 137(3), 1135-1142. 13. Bridle, P. & Timberlake, C.F. (1997). Anthocyanins as natural food colours selected aspects. Food Chemistry, 58(1), 103-109. 14. McCann, D., Barrett, A. & Cooper, A. (2007). Food additives and hyperactive behaviour in 3-year-old and 8/9-yearold children in the community: a randomised, double blinded, placebo-controlled trial. Lancet, 370(9598), 1560-1567. 15. Vijaya, S.N., Udayasri, P.V., Aswani, K.Y., Ravi, B.B., Phani, K.Y. &, Vijay, V.M. (2010). Advancements in the production of secondary metabolites. Journal of Natural Products, 3, 112-123. 16. Ginsburg, H. & Deharo, E. (2011). A call for using natural compounds in the development of new antimalarial treatments-An introduction. Malaria Journal, 10(1), 1-7. 17. Kim, B.J., Gibson, D.M. & Shuler, M.L. (2004). Effect of subculture and elicitation on instability of taxol production in Taxus sp. suspension cultures. Biotechnology Progress, 20(6), 1666-1673. 18. Sarfaraj, H., Sheeba, F., Saba, A., Akhlaguer, R., Iffet, Z.A. & Mohd, S. (2012). Current approaches toward production of secondary plant metabolites. Journal of Pharmacy and Bioallied Sciences, 4(1), 10-20. 19. Kiselev, K.V., Dubrovina, A.S., Veselova, M.V., Bulgakov, V.P., Fedoreyev, S.A. & Zhuravlev, Y.N. (2007). The rol-B gene-induced over production of resveratrol in Vitis amurensis transformed cells. Journal of Biotechnology, 128(3), 681-692. 20. Caponio, F., Alloggio, V. & Gomes, T. (1999). Phenolic compounds of virgin olive oil:Influence of paste preparation techniques. Food Chemistry, 64(2), 203-209. 21. Yiğit, N., Bayhan Öktem, A. & Aksu, P. (2008). Gıdalarda pestisit kalıntı analizlerinde yüksek basınç sıvı kromatografisi (HPLC)' nin kullanımı. Türkiye 10. Gıda Kongresi, 21-23 Mayıs, 2008, Erzurum, 1079-1082. 22. Gamborg, O.L., Miller, R.A. & Okajima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50(1), 151-156. 23. Shure, K. & Acree, T. (1994). Production of ß-damascenone precursors in cell cultures of Vitis labrusca cv. Concord grapes. Plant Cell Reports, 13(8), 477- 480. 24. Çetin, E.S. (2012). Gamay üzüm çeşidine ait kallus kültürlerinde fenolik bileşikler ile α-tokoferol üretiminin artırılması: Potansiyel bir elisitör olarak UV-C. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 7(2),112-122. 25. Yağcı, C., Toker, M.C. & Toker, G. (2008). Bitki doku kültürü yoluyla üretilen flavonoitler. Türk Bilimsel Derlemeler Dergisi, 1(1), 47-58. 26. Topçu, Ş. & Çölgeçen, H. (2015). Bitki sekonder metabolitlerinin biyoreaktörlerde üretilmesi. Türk Bilimsel Derlemeler Dergisi, 8(2), 9-29. 27. Curtin, C., Zhang, W. & Franco, C. (2003). Manipulating anthocyanin composition in Vitis vinifera suspension cultures by elicitation with jasmonic acid and light irradiation. Biotechnology Letters, 25(14), 1131-1135. 28. Antognoni, F., Zheng, S., Pagnucco, C., Baraldi, R., Poli, F. & Biondi, S. (2007). Induction of flavonoid production by UV-B radiation in Passiflora quadrangularis callus cultures. Fitoterapia, 78(5), 345-352. 29. Keskin, N. & Kunter, B. (2007). Erciş üzüm çeşidinin kallus kültürlerinde UV ışını etkisiyle resveratrol üretiminin uyarılması. Ankara Üniversitesi Tarım Bilimleri Dergisi, 13(4), 379-384. 30. Keskin, N. & Kunter, B. (2010). Production of trans-resveratrol in callus tissue of Öküzgözü (Vitis vinifera L.) in response to ultraviolet-C irradiation. Journal of Animal and Plant Sciences, 20(3), 197-200. 31. Mihai, R., Mitoi, M., Brezeanu, A. & Cogalniceanu, G. (2010). Two–stage system, a possible strategy for the enhancement of anthocyanin biosynthesis in a long-term grape callus cultures. Romanian Biotechnological Letters, 15(1), 5025-5033. 32. Aumont, V., Larronde, F., Richard, T., Budzinski, H., Decendit, A., Defieux, G. et al. (2004). Production of highly 13 C-labeled polyphenols in Vitis vinifera cell bioreactor cultures. Journal of Biotechnology, 109(3), 287-294. 33. Belhadj, A., Telef, N., Saigne, C., Cluzet, S., Barrieu, F., Hamdi, S. et al. (2008). Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures. Plant Physiology and Biochemistry, 46(4), 493-499.

Production of Anthocyanins by Biotechnological Methods

Year 2020, Volume: 4 Issue: 1, 1 - 9, 31.01.2020

Abstract

Objective: Plants produce some
secondary compounds which are used in medicine, food, cosmetics and textile
industries. Some of these compounds are effective in humans such as
antioxidant, antiinflammatory, antialergic, antiulcer, antibiotic and
anticarcinogenic therefore the interest in plants that intensively produce
these compounds has increased. It is important that to obtain these compounds
on high levels, but it is known that they are obtained at lower levels by
traditional extraction methods.

Methods:
These compounds can be obtained with
high-quality, quantity and purity by biotechnological methods. Therefore,
studies have focused on these techniques. In this study, it was aimed that the
production of anthocyanins by callus culture obtained from the leaves of the
Alphonse Lavalle grape variety and it was also aimed the determination of these
compounds with HPLC.

Results: As a result of this research, six media were used and
the anthocyanin contents were changed between 8.716 and 94.501 µg/g.

Conclusion: It is important to obtain high-value and sustainable
production of the components which are highly valuable for human health and
which have high added value without damaging the environment with tissue
culture techniques and different applications.

References

  • 1. Birudu, R.B. & Naik, M.J. (2014). Anticancer properties of secondary metabolites of medicinal plants in carcinoma. British Biomedical Bulletin, 2(4), 662-668. 2. Kennedy, D.O. (2014). Polyphenols and the human brain: Plant “secondary metabolite” ecologic roles and endogenous signaling functions drive benefits. American Society for Nutrition Advances in Nutrition, 5(5), 515-533. 3. Wink, M. (2015). Modes of action of herbal medicines and plant secondary metabolites. Medicines, 2(3), 251-286. 4. Luciano, A.J., Irineo, T.P., Rosalía Virginia, O.V., Feregrino-Pérez, A.A., Hernández, A.C. & Ramón Gerardo, G.G. (2017). Integrating plant nutrients and elicitors for production of secondary metabolites, sustainable crop production and human health: A Review. International Journal of Agriculture and Biology, 19(3), 391-402. 5. Decendit, A. & Merillon, J.M. (1996). Condensed tannin and anthocyanin production in Vitis vinifera cell suspension cultures. Plant Cell Reports, 15(10), 762-765. 6. Vitrac, X., Krisa, S., Decendit, A., Vercauteren, J., Nührich, A., Monti, J.P. et al. (2002). Carbon-14 biolabelling of wine polyphenols in Vitis vinifera cell suspension cultures. Journal of Biotechnology, 95(1), 49-56. 7. Waterhouse, A.L. (1995). Wine and heart disease. Chemical Industry, 9, 338-341. 8. Merillon, J.M., Fauconneau, B., Waffo Teguo, P., Barrier, L., Vercauteren, J. & Huguet, F. (1997). Antioxidant activity of the stilbene astringin, newly extracted from Vitis vinifera cell cultures. Clinical Chemistry, 43(6), 1092-1093. 9. Jennings, A., Welch, A.A., Spector, T., Macgregor, A. & Cassidy, A. (2014). Intakes of anthocyanins and flavones are associated with biomarkers of insulin resistance and inflammation in women. Journal of Nutrition, 144(2), 202-208. 10. Khoo, H.E., Azlan, A., Tang, S.T. & Lim, S.M. (2017). Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Journal of Food and Nutrition Research, 61(1), 1-21. 11. Juranić, Z. & Zizak, Z. (2005). Biological activities of berries: from antioxidant capacity to anti-cancer effects. Biofactors, 23(4), 207-211. 12. He, K, Li, X. & Chen, X. (2011). Evaluation of antidiabetic potential of selected traditional Chinese medicines in STZ-induced diabetic mice. Journal of Ethnopharmacology, 137(3), 1135-1142. 13. Bridle, P. & Timberlake, C.F. (1997). Anthocyanins as natural food colours selected aspects. Food Chemistry, 58(1), 103-109. 14. McCann, D., Barrett, A. & Cooper, A. (2007). Food additives and hyperactive behaviour in 3-year-old and 8/9-yearold children in the community: a randomised, double blinded, placebo-controlled trial. Lancet, 370(9598), 1560-1567. 15. Vijaya, S.N., Udayasri, P.V., Aswani, K.Y., Ravi, B.B., Phani, K.Y. &, Vijay, V.M. (2010). Advancements in the production of secondary metabolites. Journal of Natural Products, 3, 112-123. 16. Ginsburg, H. & Deharo, E. (2011). A call for using natural compounds in the development of new antimalarial treatments-An introduction. Malaria Journal, 10(1), 1-7. 17. Kim, B.J., Gibson, D.M. & Shuler, M.L. (2004). Effect of subculture and elicitation on instability of taxol production in Taxus sp. suspension cultures. Biotechnology Progress, 20(6), 1666-1673. 18. Sarfaraj, H., Sheeba, F., Saba, A., Akhlaguer, R., Iffet, Z.A. & Mohd, S. (2012). Current approaches toward production of secondary plant metabolites. Journal of Pharmacy and Bioallied Sciences, 4(1), 10-20. 19. Kiselev, K.V., Dubrovina, A.S., Veselova, M.V., Bulgakov, V.P., Fedoreyev, S.A. & Zhuravlev, Y.N. (2007). The rol-B gene-induced over production of resveratrol in Vitis amurensis transformed cells. Journal of Biotechnology, 128(3), 681-692. 20. Caponio, F., Alloggio, V. & Gomes, T. (1999). Phenolic compounds of virgin olive oil:Influence of paste preparation techniques. Food Chemistry, 64(2), 203-209. 21. Yiğit, N., Bayhan Öktem, A. & Aksu, P. (2008). Gıdalarda pestisit kalıntı analizlerinde yüksek basınç sıvı kromatografisi (HPLC)' nin kullanımı. Türkiye 10. Gıda Kongresi, 21-23 Mayıs, 2008, Erzurum, 1079-1082. 22. Gamborg, O.L., Miller, R.A. & Okajima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50(1), 151-156. 23. Shure, K. & Acree, T. (1994). Production of ß-damascenone precursors in cell cultures of Vitis labrusca cv. Concord grapes. Plant Cell Reports, 13(8), 477- 480. 24. Çetin, E.S. (2012). Gamay üzüm çeşidine ait kallus kültürlerinde fenolik bileşikler ile α-tokoferol üretiminin artırılması: Potansiyel bir elisitör olarak UV-C. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 7(2),112-122. 25. Yağcı, C., Toker, M.C. & Toker, G. (2008). Bitki doku kültürü yoluyla üretilen flavonoitler. Türk Bilimsel Derlemeler Dergisi, 1(1), 47-58. 26. Topçu, Ş. & Çölgeçen, H. (2015). Bitki sekonder metabolitlerinin biyoreaktörlerde üretilmesi. Türk Bilimsel Derlemeler Dergisi, 8(2), 9-29. 27. Curtin, C., Zhang, W. & Franco, C. (2003). Manipulating anthocyanin composition in Vitis vinifera suspension cultures by elicitation with jasmonic acid and light irradiation. Biotechnology Letters, 25(14), 1131-1135. 28. Antognoni, F., Zheng, S., Pagnucco, C., Baraldi, R., Poli, F. & Biondi, S. (2007). Induction of flavonoid production by UV-B radiation in Passiflora quadrangularis callus cultures. Fitoterapia, 78(5), 345-352. 29. Keskin, N. & Kunter, B. (2007). Erciş üzüm çeşidinin kallus kültürlerinde UV ışını etkisiyle resveratrol üretiminin uyarılması. Ankara Üniversitesi Tarım Bilimleri Dergisi, 13(4), 379-384. 30. Keskin, N. & Kunter, B. (2010). Production of trans-resveratrol in callus tissue of Öküzgözü (Vitis vinifera L.) in response to ultraviolet-C irradiation. Journal of Animal and Plant Sciences, 20(3), 197-200. 31. Mihai, R., Mitoi, M., Brezeanu, A. & Cogalniceanu, G. (2010). Two–stage system, a possible strategy for the enhancement of anthocyanin biosynthesis in a long-term grape callus cultures. Romanian Biotechnological Letters, 15(1), 5025-5033. 32. Aumont, V., Larronde, F., Richard, T., Budzinski, H., Decendit, A., Defieux, G. et al. (2004). Production of highly 13 C-labeled polyphenols in Vitis vinifera cell bioreactor cultures. Journal of Biotechnology, 109(3), 287-294. 33. Belhadj, A., Telef, N., Saigne, C., Cluzet, S., Barrieu, F., Hamdi, S. et al. (2008). Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures. Plant Physiology and Biochemistry, 46(4), 493-499.
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Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research Articles
Authors

Hale Canbay 0000-0002-3783-8064

Emine Sema Çetin 0000-0001-7601-8491

Selda Daler 0000-0003-0422-1444

Publication Date January 31, 2020
Published in Issue Year 2020 Volume: 4 Issue: 1

Cite

APA Canbay, H., Çetin, E. S., & Daler, S. (2020). Antosiyaninlerin Biyoteknolojik Yöntemlerle Üretimi. Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, 4(1), 1-9.