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The Effects of Melatonin and Vitamin E on Antioxidant Parameters in Copper Nephrotoxicity

Year 2018, Volume: 13 Issue: 3, 347 - 354, 25.12.2018
https://doi.org/10.17094/ataunivbd.370501

Abstract

Copper (Cu) compounds have been widely used in various fields especially industrial processes and agriculture.

Although trace amounts of Cu are essential to sustain life, excessive Cu is toxic for living organisms. This study aims to

investigate the effects of vitamin E and melatonin on antioxidant enzymes activities in kidney against acute Cu toxicity. In this

study, 28 Wistar albino rats were divided four equal groups (n=7) as control, Cu, Cu+Vitamin E, and Cu+Melatonin groups.

Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) enzymes activities and protein carbonyl content

were analyzed. Also, kidney function tests were evaluated. While Cu administration increased creatinine, blood urea nitrogen

(BUN), uric acid, Na, K and Mg levels, vitamin E and melatonin was effective reduction on these levels. Carbonyl level

significantly decreased in Cu+Vitamin E and Cu+melatonin groups compared to Cu group. Although SOD, GPx and CAT

activities in tissue decreased by Cu exposure, vitamin E and melatonin treatment showed significant increase on their

activities. Consequently, vitamin E and melatonin supplements may provide prevention Cu -based oxidation by strengthening

the antioxidant defense system. In this respect, it may show a protective effect by preventing the development of

nephrotoxicity.

References

  • 1. Dietary Reference Intakes., 2001. Dietary Reference Intakes. Institute of Medicine (US) Panel on Micronutrients. Washington (DC): National Academies Press (US). Chapter 7-Copper pp. 224-257.
  • 2. WHO 2003. Copper in drinking-water. Background document for preparation of WHO Guidelines for drinking-water quality. Geneva, World Health Organization (WHO/SDE/WSH/03.04/88).
  • 3. Arnal N., Astiz M., Alaniz de MJT., Marra CA., 2011. Clinical parameters and biomarkers of oxidative stress in agricultural workers who applied copper-based pesticides. Ecotoxicol Environ Saf, 74, 1779-1786.
  • 4. Cockell KA., Bertinato J., LÁbbé MR., 2008. Regulatory frameworks for copper considering chronic exposures of the population. Am J Clin Nutr, 88, 863S-866S.
  • 5. Brewer GJ., 2012. Metals in the causation and treatment of Wilson’s disease and Alzheimer’s disease, and copper lowering therapy in medicine. Inorg Chim Acta, 393, 135-141.
  • 6. Gaetke LM., Chow CK., 2003. Copper toxicity, oxidative stress, and antioxidant nutrients. Toxicology, 189 (1-2), 147-163.
  • 7. Valko M., Morris H., Cronin MT., 2005. Metals, toxicity and oxidative stress. Curr Med Chem, 12, 1161-1208.
  • 8. Pham-Huy LA., He H., Pham-Huy C., 2008. Free radicals, antioxidants in disease and health. Int J Biomed Sci, 4 (2), 89-96.
  • 9. Combs GF., McClung JP., 2017. The Vitamins: Fundamental aspects in nutrition and health. Academic Press, California.
  • 10. Carrillo-Vico A., Lardone PJ., Álvarez-Sánchez N., Rodríguez-Rodríguez A., Guerrero JM., 2013. Melatonin: Buffering the Immune System. Int J Mol Sci, 14, 8638-8683.
  • 11. Cutando A., Lopez-Valverde A., Arias-Santiago S., de Vicente J., de Diego RG., 2012. Role of melatonin in cancer treatment. Anticancer Res, 32, 2747-2753.
  • 12. Hardeland R., Cardinali DP., Srinivasan V., Spence DW., Brown GM., Pandi-Perumal SR., 2011. Melatonin—A pleiotropic, orchestrating regulator molecule. Prog Neurobiol, 93, 350-384.
  • 13. Saleha-Banu B., Ishaq M., Danadevi K., Padmavathi P., Ahuja YR., 2004. DNA damage in leukocytes of mice treated with copper sulfate. Food Chem Toxicol, 42, 1931-1936.
  • 14. Giray B., Gürbay A., Hincal F., 2001. Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol. Toxicol Lett, 118, 139-146.
  • 15. Zararsiz I., Sarsilmaz M., Tas U., Kus I., Meydan S., Ozan E., 2007. Protective effect of melatonin against formaldehyde-induced kidney damage in rats. Toxicol Ind Health, 23, 573-579.
  • 16. Levine RL., Garland D., Oliver CN., Amici A., Climent I., Lenz AG., Ahn BW., Shaltiel S., Stadtman ER., 1990. Determination of carbonyl content in oxidatively modified proteins. Method Enzymol, 186, 464-478.
  • 17. McCord JM., Fridovich I., 1969. Superoxide Dismutase. An Enzymic Function for Erythrocuprein (Hemocuprein). J Biol Chem, 244, 6049-6055.
  • 18. Paglia DE., Valentine WN., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70 (1), 158-169.
  • 19. Aebi H., 1984. Catalase in vitro. Method Enzymol, 105. 121-126.
  • 20. Lowry OH., Rosebrough WI., Farr AL., Randall RJ., 1951. Protein measurement with the folin phenol reagent. J Biol Chem, 193, 265-275.
  • 21. Lifton RP., Somlo S., Giebisch GH., Seldin DW., 2009. Genetic Diseases of the Kidney. Academic Press, New York. 710-711.
  • 22. Lei R., Wu C., Yang B., Ma H., Shi C., Wang Q., Wang Q., Yuan Y., Liao M., 2008. Integrated metabolomic analysis of the nano-sized copper particle-induced hepatotoxicity and nephrotoxicity in rats. A rapid in vivo screening method for nanotoxicity. Toxicol App Pharmacol, 232, 292-301.
  • 23. Kumar V., Kalita J., Misra UK., Bora HK., 2015. A study of dose response and organ susceptibility of copper toxicity in a rat model. Journal of Trace Elements in Medicine and Biology, 29. 269-274.
  • 24. Osfor MMH., Ibrahim HS., Mohamed YA., Ahmed AM., Abd El Azeem AS., Hegazy AM., 2010. Effect of alpha lipoic acid and vitamin E on heavy metals intoxication in male albino rats. J Am Sci, 6, 56-63.
  • 25. Ahmadizadeh M; Baghpa AR., 2008. The preventive effect of vitamin E on cadmium chloride-induced toxicity in rat liver and kidney. Jundishapur Scientific Medical Journal, 6, 404-413.
  • 26. Stadtman ER 2006. Protein oxidation and aging. Free Rad Res 40 (12), 1250-1258.
  • 27. Arnal N., Dominici L., de Tacconi MTJ., Marra CA .,2014. Copper-induced alterations in rat brain depends on route of overload and basal copper levels. Nutrition, 30, 96-106.
  • 28. Parmar P., Limson J., Nyokong T., Daya S., 2002. Melatonin protects against copper-mediated free radical damage. J Pineal Res, 32, 237-242.
  • 29. Zhang SSZ., Noordin MM., Rahman SOA., Haron MJ., 2000. Effects of copper overload on hepatic lipid peroxidation and antioxidant defense in rats. Vet Hum Toxicol, 42, 261-264.
  • 30. Dalle-Donne I., Rossi R., Giustarini D., Milzani A., Colombo R., 2003. Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta, 329, 23-38.
  • 31. Nyström T., 2005. Role of oxidative carbonylation in protein quality control and senescence. EMBO J, 24, 1311-1317.
  • 32. Speisky H., Gómez M., Burgos-Bravo F., López-Alarcón C., Jullian C., Olea-Azar C., Aliaga ME., 2009. Generation of superoxide radicals by copper–glutathione complexes: Redox-consequences associated with their interaction with reduced glutathione. Bioorg Med Chem, 17, 1803-1810.
  • 33. Ajith TA., Usha S., Nivitha V., 2007. Ascorbic acid and α-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clinica Chimica Acta, 375, 82-86.
  • 34. Ossola JO., Groppa MD., Tomaro ML., 1997. Relationship between oxidative stress and heme oxygenase induction by copper sulfate. Arch Biochem Biophys, 337 (2), 332-337.
  • 35. El-Sokkary GH., Abdel-Rahman GH., Kamel ES., 2005. Melatonin protects against lead-induced hepatic and renal toxicity in male rats. Toxicology, 213, 25-33.
  • 36. Mayo JC., Sainz RM., Antolín I., Herrera F., Martin V., Rodriguez C., 2002. Melatonin regulation of antioxidant enzyme gene expression. Cell Mol Life Sci, 59, 1706-1713.
  • 37. Reiter RJ., Tan DX., Osuna C., Gitto E., 2000. Actions of melatonin in the reduction of oxidative stress. J Biomed Sci, 7, 444-458.
  • 38. Sajitha GR., Jose R., Andrews A., Ajantha KG., Augustine P., Augusti KT., 2010. Garlic oil and vitamin e prevent the adverse effects of lead acetate and ethanol separately as well as in combination in the drinking water of rats. Indian J Clin Biochem, 25 (3), 280-288.
  • 39. Zhang H., Zhang Y., 2014. Melatonin: a well-documented antioxidant with conditional pro-oxidant actions. Pineal Res, 57, 131-146.
  • 40. Emamgholipour S., Hossein-Nezhad A., Ansari M., 2016. Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells? Biochem Res Int, 2016, Article ID 5857940 1-8.

Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri

Year 2018, Volume: 13 Issue: 3, 347 - 354, 25.12.2018
https://doi.org/10.17094/ataunivbd.370501

Abstract

Bakır (Cu) bileşikleri özellikle endüstri ve tarımda geniş kullanım alanına sahiptir. İz miktardaki Cu hayatın sürdürülebilir

olması için gerekliyken fazlası organizma için toksik etki yapmaktadır. Bu çalışma akut Cu toksisitesinde melatonin ve E

vitamini’nin böbreklerdeki antioksidan enzim aktivitelerine etkisinin araştırılması amacıyla planlanmıştır. Çalışmada 28 Wistar

albino sıçanlar kontrol grubu, Cu grubu, Cu+E vitamini grubu ve Cu+Melatonin grubu şeklinde eşit dört gruba (n=7) ayrıldı.

Böbrek dokusunda süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (KAT) enzim aktiviteleri ve protein karbonil

içeriği analiz edildi. Ayrıca böbrek fonksiyon testleri değerlendirildi. Bakır uygulaması, kreatinin, kan üre nitrojeni (BUN), ürik

asit, Na, K ve Mg değerlerini artırırken, E vitamini ve melatonin bu değerlerin azalmasında etkili oldu. Karbonil seviyesi, Cu+E

vitamini veya Cu+melatonin gruplarında Cu grubuna göre önemli derecede azaldı. Doku SOD, GPx ve KAT aktiviteleri Cu

maruziyeti sonucu düşmesine rağmen; E vitamini veya melatonin uygulaması, bu aktivitelerde önemli bir artışa neden oldu.

Sonuç olarak, E vitamini ve melatonin takviyesi antioksidan sistemi güçlendirerek Cu temelli oksidasyonun engellenmesini

sağlayabilir. Bu sayede nefrotoksisitenin gelişmesini önleyerek koruyucu bir fonksiyon gösterebilir.

References

  • 1. Dietary Reference Intakes., 2001. Dietary Reference Intakes. Institute of Medicine (US) Panel on Micronutrients. Washington (DC): National Academies Press (US). Chapter 7-Copper pp. 224-257.
  • 2. WHO 2003. Copper in drinking-water. Background document for preparation of WHO Guidelines for drinking-water quality. Geneva, World Health Organization (WHO/SDE/WSH/03.04/88).
  • 3. Arnal N., Astiz M., Alaniz de MJT., Marra CA., 2011. Clinical parameters and biomarkers of oxidative stress in agricultural workers who applied copper-based pesticides. Ecotoxicol Environ Saf, 74, 1779-1786.
  • 4. Cockell KA., Bertinato J., LÁbbé MR., 2008. Regulatory frameworks for copper considering chronic exposures of the population. Am J Clin Nutr, 88, 863S-866S.
  • 5. Brewer GJ., 2012. Metals in the causation and treatment of Wilson’s disease and Alzheimer’s disease, and copper lowering therapy in medicine. Inorg Chim Acta, 393, 135-141.
  • 6. Gaetke LM., Chow CK., 2003. Copper toxicity, oxidative stress, and antioxidant nutrients. Toxicology, 189 (1-2), 147-163.
  • 7. Valko M., Morris H., Cronin MT., 2005. Metals, toxicity and oxidative stress. Curr Med Chem, 12, 1161-1208.
  • 8. Pham-Huy LA., He H., Pham-Huy C., 2008. Free radicals, antioxidants in disease and health. Int J Biomed Sci, 4 (2), 89-96.
  • 9. Combs GF., McClung JP., 2017. The Vitamins: Fundamental aspects in nutrition and health. Academic Press, California.
  • 10. Carrillo-Vico A., Lardone PJ., Álvarez-Sánchez N., Rodríguez-Rodríguez A., Guerrero JM., 2013. Melatonin: Buffering the Immune System. Int J Mol Sci, 14, 8638-8683.
  • 11. Cutando A., Lopez-Valverde A., Arias-Santiago S., de Vicente J., de Diego RG., 2012. Role of melatonin in cancer treatment. Anticancer Res, 32, 2747-2753.
  • 12. Hardeland R., Cardinali DP., Srinivasan V., Spence DW., Brown GM., Pandi-Perumal SR., 2011. Melatonin—A pleiotropic, orchestrating regulator molecule. Prog Neurobiol, 93, 350-384.
  • 13. Saleha-Banu B., Ishaq M., Danadevi K., Padmavathi P., Ahuja YR., 2004. DNA damage in leukocytes of mice treated with copper sulfate. Food Chem Toxicol, 42, 1931-1936.
  • 14. Giray B., Gürbay A., Hincal F., 2001. Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol. Toxicol Lett, 118, 139-146.
  • 15. Zararsiz I., Sarsilmaz M., Tas U., Kus I., Meydan S., Ozan E., 2007. Protective effect of melatonin against formaldehyde-induced kidney damage in rats. Toxicol Ind Health, 23, 573-579.
  • 16. Levine RL., Garland D., Oliver CN., Amici A., Climent I., Lenz AG., Ahn BW., Shaltiel S., Stadtman ER., 1990. Determination of carbonyl content in oxidatively modified proteins. Method Enzymol, 186, 464-478.
  • 17. McCord JM., Fridovich I., 1969. Superoxide Dismutase. An Enzymic Function for Erythrocuprein (Hemocuprein). J Biol Chem, 244, 6049-6055.
  • 18. Paglia DE., Valentine WN., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70 (1), 158-169.
  • 19. Aebi H., 1984. Catalase in vitro. Method Enzymol, 105. 121-126.
  • 20. Lowry OH., Rosebrough WI., Farr AL., Randall RJ., 1951. Protein measurement with the folin phenol reagent. J Biol Chem, 193, 265-275.
  • 21. Lifton RP., Somlo S., Giebisch GH., Seldin DW., 2009. Genetic Diseases of the Kidney. Academic Press, New York. 710-711.
  • 22. Lei R., Wu C., Yang B., Ma H., Shi C., Wang Q., Wang Q., Yuan Y., Liao M., 2008. Integrated metabolomic analysis of the nano-sized copper particle-induced hepatotoxicity and nephrotoxicity in rats. A rapid in vivo screening method for nanotoxicity. Toxicol App Pharmacol, 232, 292-301.
  • 23. Kumar V., Kalita J., Misra UK., Bora HK., 2015. A study of dose response and organ susceptibility of copper toxicity in a rat model. Journal of Trace Elements in Medicine and Biology, 29. 269-274.
  • 24. Osfor MMH., Ibrahim HS., Mohamed YA., Ahmed AM., Abd El Azeem AS., Hegazy AM., 2010. Effect of alpha lipoic acid and vitamin E on heavy metals intoxication in male albino rats. J Am Sci, 6, 56-63.
  • 25. Ahmadizadeh M; Baghpa AR., 2008. The preventive effect of vitamin E on cadmium chloride-induced toxicity in rat liver and kidney. Jundishapur Scientific Medical Journal, 6, 404-413.
  • 26. Stadtman ER 2006. Protein oxidation and aging. Free Rad Res 40 (12), 1250-1258.
  • 27. Arnal N., Dominici L., de Tacconi MTJ., Marra CA .,2014. Copper-induced alterations in rat brain depends on route of overload and basal copper levels. Nutrition, 30, 96-106.
  • 28. Parmar P., Limson J., Nyokong T., Daya S., 2002. Melatonin protects against copper-mediated free radical damage. J Pineal Res, 32, 237-242.
  • 29. Zhang SSZ., Noordin MM., Rahman SOA., Haron MJ., 2000. Effects of copper overload on hepatic lipid peroxidation and antioxidant defense in rats. Vet Hum Toxicol, 42, 261-264.
  • 30. Dalle-Donne I., Rossi R., Giustarini D., Milzani A., Colombo R., 2003. Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta, 329, 23-38.
  • 31. Nyström T., 2005. Role of oxidative carbonylation in protein quality control and senescence. EMBO J, 24, 1311-1317.
  • 32. Speisky H., Gómez M., Burgos-Bravo F., López-Alarcón C., Jullian C., Olea-Azar C., Aliaga ME., 2009. Generation of superoxide radicals by copper–glutathione complexes: Redox-consequences associated with their interaction with reduced glutathione. Bioorg Med Chem, 17, 1803-1810.
  • 33. Ajith TA., Usha S., Nivitha V., 2007. Ascorbic acid and α-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clinica Chimica Acta, 375, 82-86.
  • 34. Ossola JO., Groppa MD., Tomaro ML., 1997. Relationship between oxidative stress and heme oxygenase induction by copper sulfate. Arch Biochem Biophys, 337 (2), 332-337.
  • 35. El-Sokkary GH., Abdel-Rahman GH., Kamel ES., 2005. Melatonin protects against lead-induced hepatic and renal toxicity in male rats. Toxicology, 213, 25-33.
  • 36. Mayo JC., Sainz RM., Antolín I., Herrera F., Martin V., Rodriguez C., 2002. Melatonin regulation of antioxidant enzyme gene expression. Cell Mol Life Sci, 59, 1706-1713.
  • 37. Reiter RJ., Tan DX., Osuna C., Gitto E., 2000. Actions of melatonin in the reduction of oxidative stress. J Biomed Sci, 7, 444-458.
  • 38. Sajitha GR., Jose R., Andrews A., Ajantha KG., Augustine P., Augusti KT., 2010. Garlic oil and vitamin e prevent the adverse effects of lead acetate and ethanol separately as well as in combination in the drinking water of rats. Indian J Clin Biochem, 25 (3), 280-288.
  • 39. Zhang H., Zhang Y., 2014. Melatonin: a well-documented antioxidant with conditional pro-oxidant actions. Pineal Res, 57, 131-146.
  • 40. Emamgholipour S., Hossein-Nezhad A., Ansari M., 2016. Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells? Biochem Res Int, 2016, Article ID 5857940 1-8.
There are 40 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Araştırma Makaleleri
Authors

Mehmet Ali Temiz

Atilla Temur

Publication Date December 25, 2018
Published in Issue Year 2018 Volume: 13 Issue: 3

Cite

APA Temiz, M. A., & Temur, A. (2018). Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13(3), 347-354. https://doi.org/10.17094/ataunivbd.370501
AMA Temiz MA, Temur A. Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. December 2018;13(3):347-354. doi:10.17094/ataunivbd.370501
Chicago Temiz, Mehmet Ali, and Atilla Temur. “Bakır Nefrotoksisitesinde Melatonin Ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13, no. 3 (December 2018): 347-54. https://doi.org/10.17094/ataunivbd.370501.
EndNote Temiz MA, Temur A (December 1, 2018) Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13 3 347–354.
IEEE M. A. Temiz and A. Temur, “Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 13, no. 3, pp. 347–354, 2018, doi: 10.17094/ataunivbd.370501.
ISNAD Temiz, Mehmet Ali - Temur, Atilla. “Bakır Nefrotoksisitesinde Melatonin Ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13/3 (December 2018), 347-354. https://doi.org/10.17094/ataunivbd.370501.
JAMA Temiz MA, Temur A. Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018;13:347–354.
MLA Temiz, Mehmet Ali and Atilla Temur. “Bakır Nefrotoksisitesinde Melatonin Ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 13, no. 3, 2018, pp. 347-54, doi:10.17094/ataunivbd.370501.
Vancouver Temiz MA, Temur A. Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018;13(3):347-54.