Abstract
Ischemia-reperfusion injury is a clinical condition that poses a life-threatening risk due to certain diseases or operations such as trauma, shock, and gastric dilatation volvulus. The study's objective was to investigate the impact of oxytocin on intestinal damage in rats caused by ischemia-reperfusion injury induced experimentally. Three groups of Wistar albino rats were formed: Control (CTR, n=6), intestinal ischemia-reperfusion (I-IR, n=6), and intestinal ischemia-reperfusion+ Oxytocin (I-IR+Oxt, n=6). Oxytocin (1 mg/kg) was given intraperitoneally to the I-IR+Oxt group 30 min prior to the anesthesia. In the I-IR and I-IR+Oxt groups, ischemia reperfusion was performed with 1 h ligation of the superior mesenteric artery and one hour reperfusion by opening the ligatures. After the end of the reperfusion period, euthanasia was performed and blood and intestinal tissue samples were taken. From the blood samples, ALT, ALP, AST, LDH, BUN, creatinine, IL-1β, TNF-α, concentrations and from the tissue sample, IL-1β, TNF-α and MDA activities were evaluated. While serum and tissue IL-1β, TNF-α concentrations were greater in both I-IR and I-IR+Oxt groups compared to the CTR group, these levels were determined to be lower in the I-IR+Oxt group compared to the I-IR group. Histopathological analysis showed that epithelial regeneration of both epithelium and glands structure and decreased inflammatory cell infiltration were found in the I-IR+Oxt group compared to the I-I/R group. In conclusion, oxytocin inhibited the release of IL-1β and TNF-α and the harmful effect of IR on intestinal cells.
Supporting Institution
-
Project Number
-
Thanks
-
References
- Alexandropoulos D, Bazigos GV, Doulamis IP, et al (2017): Protective effects of N-acetylcysteine and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion. Biomed Pharmacother, 89, 673-680.
- Asad M, Shewade DG, Koumaravelou K, et al (2001): Gastric antisecretory and antiulcer activity of oxytocin in rats and guinea pigs. Life Sci, 70, 17–24.
- Bradford MM (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248-254.
- Braun JP, Lefebvre HP (2008): Kidney function and damage. 485-529. In: JJ Kaneko, JW Harvey, ML Bruss (Eds.), Clinical Biochemistry of Domestic Animals. Elsevier Academic Press, Burlington.
- Carter CS, Kenkel WM, MacLean EL, et al (2020): Is oxytocin “nature’s medicine”? Pharmacol Rev, 72, 829-861.
- Cassutto BH, Gfeller RW (2003): Use of intravenous lidocaine to prevent reperfusion injury and subsequent multiple organ dysfunction syndrome. J Vet Emerg Crit Care, 13, 137-148.
- Çetinel Ş, Hancıoğlu S, Şener E, et al (2010): Oxytocin treatment alleviates stress-aggravated colitis by a receptor-dependent mechanism. Regul Pept, 160, 146-152.
- Deng F, Lin ZB, Sun QS, et al (2022): The role of intestinal microbiota and its metabolites in intestinal and extraintestinal organ injury induced by intestinal ischemia-reperfusion injury. Int J Biol Sci, 18, 3981-3992.
- Düşünceli F, İşeri SÖ, Ercan F, et al (2008): Oxytocin alleviates hepatic ischemia–reperfusion injury in rats. Peptides, 29, 1216-1222.
- ELKady AH, Elkafoury BM, Saad DA, et al (2021): Hepatic ischemia-reperfusion injury: effect of moderate-intensity exercise and oxytocin compared to l-arginine in a rat model. Egypt Liver J, 11, 1-15
- Gardner AK, Schroeder EL (2022): Pathophysiology of intraabdominal hypertension and abdominal compartment syndrome and relevance to veterinary critical care. J Vet Emerg Crit Care, 32, 48-56.
- Gharishvandi F, Abdollahi A, Shafaroodi H, et al (2020): Involvement of 5-HT1B/1D receptors in the inflammatory response and oxidative stress in intestinal ischemia/reperfusion in rats. Eur J Pharmacol, 882, 173265.
- Gonzalez LM, Moeser AJ, Blikslager AT (2015): Animal models of ischemia-reperfusion-induced intestinal injury: Progress and promise for translational research. Am J Physiol Gastrointest Liver Physiol, 308, G63–G75.
- Gregová K, Číkoš Š, Bilecová-Rabajdová M, et al (2015): Intestinal ischemia-reperfusion injury mediates expression of inflammatory cytokines in rats. Gen Physiol Biophys, 34, 95-99.
- Hamilton TR, Thacher CW, Forsee KM, et al (2010): Trauma‐associated acute mesenteric ischemia in a dog. J Vet Emerg Crit Care, 20, 595-600.
- Lapsekili E, Menteş Ö, Balkan M, et al (2016): Role of alkaline phosphatase intestine-isomerase in acute mesenteric ischemia diagnosis. TJTES, 22, 115-120.
- McMichael M, Moore RM (2004): Ischemia–reperfusion injury pathophysiology, part I. J Vet Emerg Crit Care, 14, 231-241.
- Nadatani Y, Watanabe T, Shimada S, et al (2018): Microbiome and intestinal ischemia/reperfusion injury. J Clin Biochem Nutr, 63, 26-32.
- Ohkawa H, Ohishi N, Yagi K (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351–358.
- Oliveira-Pelegrin GR, Saia RS, Cárnio EC, et al (2013): Oxytocin affects nitric oxide and cytokine production by sepsis-sensitized macrophages. Neuroimmunomodulation, 20, 65-71.
- Pergel A, Kanter M, Yucel AF, et al (2012): Anti-inflammatory and antioxidant effects of infliximab in a rat model of intestinal ischemia/reperfusion injury. Toxicol Ind Health, 28, 923-932.
- Powell A, Armstrong P (2014): Plasma biomarkers for early diagnosis of acute intestinal ischemia. Semin Vasc Surg, 27, 170-175.
- Ragy MM, Aziz NM (2017): Prevention of renal ischemia/perfusion-induced renal and hepatic injury in adult male Albino rats by oxytocin: Role of nitric oxide. J Basic Clin Physiol Pharmacol, 28, 615-621.
- Sayıner S, Gülmez N, Sabit ZA, et al (2019): Effects of Deep-Frying Sunflower Oil on Sperm Parameters In A Mouse Model: Do Probiotics Have A Protective Effect? Kafkas Univ Vet Fak Derg, 25, 857-863.
- Şehirli AÖ, Sayiner S, Savtekin G, et al (2021): Protective effect of bromelain on corrosive burn in rats. Burns, 47, 1352-1358.
- Senturk GE, Erkanli K, Aydin U, et al (2013): The protective effect of oxytocin on ischemia/reperfusion injury in rat urinary bladder. Peptides, 40, 82-88.
- Soares RO, Losada DM, Jordan MC, et al (2019): Ischemia/reperfusion injury revisited: an overview of the latest pharmacological strategies. Int J Mol Sci, 20, 5034.
- Sookoian S, Pirola CJ (2015): Liver enzymes, metabolomics, and genome-wide association studies: from systems biology to the personalized medicine. World J Gastroenterol, 21, 711-725.
- Tuğtepe H, Şener G, Bıyıklı NK, et al (2007): The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. Regul Pept, 140, 101-108.
- VanderBroek AR, Engiles JB, Kästner SB, et al (2021): Protective effects of dexmedetomidine on small intestinal ischemia-reperfusion injury in horses. Equine Vet J, 53, 569-578.
Abstract
İskemi-reperfüzyon yaralanması, travma, şok, gastrik dilatasyon volvulus gibi bazı hastalıklar veya operasyonlar nedeniyle yaşamı tehdit eden bir klinik durumdur. Çalışmanın amacı, oksitosinin sıçanlarda deneysel olarak oluşturulan iskemi-reperfüzyon hasarının neden olduğu bağırsak hasarı üzerindeki etkisini araştırmaktı. Üç grup Wistar albino sıçan oluşturuldu: Kontrol (CTR, n=6), bağırsak iskemi-reperfüzyon (I-IR, n=6) ve bağırsak iskemi-reperfüzyon+ Oksitosin (I-IR+Oxt, n=6). I-IR+Oxt grubuna anesteziden 30 dakika önce intraperitoneal olarak oksitosin (1 mg/kg) verildi. I-IR ve I-IR+Oxt gruplarında 1 saat superior mezenterik arter ligasyonu ve ligatürler açılarak 1 saat reperfüzyon ile iskemi reperfüzyonu uygulandı. Reperfüzyon süresinin bitiminden sonra ötenazi yapılarak kan ve barsak doku örnekleri alındı. Kan örneklerinden ALT, ALP, AST, LDH, BUN, kreatinin, IL-1β, TNF-α, konsantrasyonları ve doku örneğinden IL-1β, TNF-α ve MDA aktiviteleri değerlendirildi. Serum ve doku IL-1β, TNF-α konsantrasyonları hem I-IR hem de I-IR+Oxt gruplarında CTR grubuna göre yüksek bulunurken, bu seviyelerin I-IR+Oxt grubunda I-IR+Oxt grubuna göre daha düşük olduğu belirlendi. I-IR grubu. Histopatolojik analiz, I-I/R grubuna kıyasla I-IR+Oxt grubunda hem epitel hem de bez yapısının epitelyal rejenerasyonunun ve inflamatuar hücre infiltrasyonunun azaldığını gösterdi. Sonuç olarak oksitosin, IL-1β ve TNF-α salınımını ve IR'nin bağırsak hücreleri üzerindeki zararlı etkisini engellemiştir.
Project Number
-
References
- Alexandropoulos D, Bazigos GV, Doulamis IP, et al (2017): Protective effects of N-acetylcysteine and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion. Biomed Pharmacother, 89, 673-680.
- Asad M, Shewade DG, Koumaravelou K, et al (2001): Gastric antisecretory and antiulcer activity of oxytocin in rats and guinea pigs. Life Sci, 70, 17–24.
- Bradford MM (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248-254.
- Braun JP, Lefebvre HP (2008): Kidney function and damage. 485-529. In: JJ Kaneko, JW Harvey, ML Bruss (Eds.), Clinical Biochemistry of Domestic Animals. Elsevier Academic Press, Burlington.
- Carter CS, Kenkel WM, MacLean EL, et al (2020): Is oxytocin “nature’s medicine”? Pharmacol Rev, 72, 829-861.
- Cassutto BH, Gfeller RW (2003): Use of intravenous lidocaine to prevent reperfusion injury and subsequent multiple organ dysfunction syndrome. J Vet Emerg Crit Care, 13, 137-148.
- Çetinel Ş, Hancıoğlu S, Şener E, et al (2010): Oxytocin treatment alleviates stress-aggravated colitis by a receptor-dependent mechanism. Regul Pept, 160, 146-152.
- Deng F, Lin ZB, Sun QS, et al (2022): The role of intestinal microbiota and its metabolites in intestinal and extraintestinal organ injury induced by intestinal ischemia-reperfusion injury. Int J Biol Sci, 18, 3981-3992.
- Düşünceli F, İşeri SÖ, Ercan F, et al (2008): Oxytocin alleviates hepatic ischemia–reperfusion injury in rats. Peptides, 29, 1216-1222.
- ELKady AH, Elkafoury BM, Saad DA, et al (2021): Hepatic ischemia-reperfusion injury: effect of moderate-intensity exercise and oxytocin compared to l-arginine in a rat model. Egypt Liver J, 11, 1-15
- Gardner AK, Schroeder EL (2022): Pathophysiology of intraabdominal hypertension and abdominal compartment syndrome and relevance to veterinary critical care. J Vet Emerg Crit Care, 32, 48-56.
- Gharishvandi F, Abdollahi A, Shafaroodi H, et al (2020): Involvement of 5-HT1B/1D receptors in the inflammatory response and oxidative stress in intestinal ischemia/reperfusion in rats. Eur J Pharmacol, 882, 173265.
- Gonzalez LM, Moeser AJ, Blikslager AT (2015): Animal models of ischemia-reperfusion-induced intestinal injury: Progress and promise for translational research. Am J Physiol Gastrointest Liver Physiol, 308, G63–G75.
- Gregová K, Číkoš Š, Bilecová-Rabajdová M, et al (2015): Intestinal ischemia-reperfusion injury mediates expression of inflammatory cytokines in rats. Gen Physiol Biophys, 34, 95-99.
- Hamilton TR, Thacher CW, Forsee KM, et al (2010): Trauma‐associated acute mesenteric ischemia in a dog. J Vet Emerg Crit Care, 20, 595-600.
- Lapsekili E, Menteş Ö, Balkan M, et al (2016): Role of alkaline phosphatase intestine-isomerase in acute mesenteric ischemia diagnosis. TJTES, 22, 115-120.
- McMichael M, Moore RM (2004): Ischemia–reperfusion injury pathophysiology, part I. J Vet Emerg Crit Care, 14, 231-241.
- Nadatani Y, Watanabe T, Shimada S, et al (2018): Microbiome and intestinal ischemia/reperfusion injury. J Clin Biochem Nutr, 63, 26-32.
- Ohkawa H, Ohishi N, Yagi K (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351–358.
- Oliveira-Pelegrin GR, Saia RS, Cárnio EC, et al (2013): Oxytocin affects nitric oxide and cytokine production by sepsis-sensitized macrophages. Neuroimmunomodulation, 20, 65-71.
- Pergel A, Kanter M, Yucel AF, et al (2012): Anti-inflammatory and antioxidant effects of infliximab in a rat model of intestinal ischemia/reperfusion injury. Toxicol Ind Health, 28, 923-932.
- Powell A, Armstrong P (2014): Plasma biomarkers for early diagnosis of acute intestinal ischemia. Semin Vasc Surg, 27, 170-175.
- Ragy MM, Aziz NM (2017): Prevention of renal ischemia/perfusion-induced renal and hepatic injury in adult male Albino rats by oxytocin: Role of nitric oxide. J Basic Clin Physiol Pharmacol, 28, 615-621.
- Sayıner S, Gülmez N, Sabit ZA, et al (2019): Effects of Deep-Frying Sunflower Oil on Sperm Parameters In A Mouse Model: Do Probiotics Have A Protective Effect? Kafkas Univ Vet Fak Derg, 25, 857-863.
- Şehirli AÖ, Sayiner S, Savtekin G, et al (2021): Protective effect of bromelain on corrosive burn in rats. Burns, 47, 1352-1358.
- Senturk GE, Erkanli K, Aydin U, et al (2013): The protective effect of oxytocin on ischemia/reperfusion injury in rat urinary bladder. Peptides, 40, 82-88.
- Soares RO, Losada DM, Jordan MC, et al (2019): Ischemia/reperfusion injury revisited: an overview of the latest pharmacological strategies. Int J Mol Sci, 20, 5034.
- Sookoian S, Pirola CJ (2015): Liver enzymes, metabolomics, and genome-wide association studies: from systems biology to the personalized medicine. World J Gastroenterol, 21, 711-725.
- Tuğtepe H, Şener G, Bıyıklı NK, et al (2007): The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. Regul Pept, 140, 101-108.
- VanderBroek AR, Engiles JB, Kästner SB, et al (2021): Protective effects of dexmedetomidine on small intestinal ischemia-reperfusion injury in horses. Equine Vet J, 53, 569-578.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Project Number | - |
| Early Pub Date | October 27, 2023 |
| Publication Date | |
| Published in Issue | Year 2024Accepted Papers |