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Association of the phenolic content, DNA protective activity and some antioxidant properties in the Achillea arabica Kotschy Flower

Year 2021, Volume: 10 Issue: 3, 773 - 783, 17.09.2021
https://doi.org/10.17798/bitlisfen.904487

Abstract

Pharmacological herbs are our first medicines in history and we take them into our bodies in different ways by consuming tea, spices, or raw. Nowadays, due to the side effects of more effective synthetic medicines, the tendency to use pharmacological plants has increased and the benefits are provided from plants in the treatment of routine diseases and to protect from them. In our daily life, stress and adverse environmental conditions may cause oxidative stress by increasing the concentration of reactive oxygen species (ROS) in a cell and oxidative stress is known to be associated with cancer and various neurodegenerative diseases such as Parkinson’s and Alzheimer’s. Antioxidants protect the cell against diseases by regulating ROS concentration and herbal phenolics are known as antioxidants and strong candidates for this. In addition, studies on the herb and active ingredient have gained more importance during the Covid-19 epidemic period as they provide important data for vaccine development studies. This study was aimed to obtain useful data with this study that can be used in advanced research. For this purpose, Achillea arabica, which one of the important members of the Asteraceae family known whose various pharmacological properties, was collected from Ağrı/Patnos and stored by converting into herbarium material. DMSO extract was prepared from the flowers of the herbarium plant and some biological activities such as the determination of some in vitro antioxidant properties, DNA protective activity, and the phenolic content by HPLC were evaluated.

Project Number

This work was not carried out under any project.

Thanks

I would like to express my gratitude to Dr. Murat Kurşat (Bitlis Eren University) for supporting me in the identification of the plant used in the study.

References

  • Alan Y., Savcı A., Koçpınar E.F., Kurşat M., Topdemir S., Karataş M., Çakmak B. 2019. Antimicrobial, antioxidant and DNA protective effects and phenolic content of Lallementia canescens (L.) Fisch. and CA Mey. and Lallementia peltata (L.) Fisch. and CA Mey. Biological Diversity and Conversation, 12 (3): 78-88.
  • Kikowska M., Dworacka M., Kędziora I., Thiem B. 2016. Eryngium creticum-ethnopharmacology, phytochemistry and pharmacological activity. A review. Revista Brasileira de Farmacognosia, 26 (3): 392-399.
  • Koc S., Isgor B.S., Isgor Y.G., Moghaddam N.S., Yildirim O. 2015. The potential medicinal value of plants from Asteraceae family with antioxidant defense enzymes as biological targets. Pharmaceutical Biology, 53 (5): 746-751.
  • Soumia B. 2018. Eryngium campestre L.: Polyphenolic and flavonoid compounds; Applications to health and disease. Polyphenols: Mechanisms of Action in Human Health and Disease Academic Press, (Second Edition): 69-79.
  • Nebija F., Stefkov G., Karapandzova M., Stafilov T., Panovska T.K., Kulevanova S. 2009. Chemical characterization and antioxidant activity of Eryngium campestre L., Apiaceae from Kosovo. Macedonian Pharmaceutical Society, Marshal Tito 13b/8, Skopje Macedonia, 23.
  • Savci A., Kocpinar E.F., Budak H., Ciftci M., Sisecioglu M. 2020. The Effects of Amoxicillin, Cefazolin, and Gentamicin Antibiotics on the Antioxidant System in Mouse Heart Tissues. Protein Peptide Letters, 27 (7): 614-622.
  • Budak H., Kocpinar E.F., Gonul N., Ceylan H., Erol H.S., Erdogan O. 2014. Stimulation of gene expression and activity of antioxidant related enzyme in Sprague Dawley rat kidney induced by long-term iron toxicity. Comperative Biochemistry Physiology C Toxicology and Pharmacology, 166: 44-50.
  • Budak H., Gonul N., Ceylan H., Kocpinar E.F. 2014. Impact of long term Fe3+ toxicity on expression of glutathione system in rat liver. Environmental Toxicology and Pharmacology, 37 (1): 365-70.
  • Savci A., Kocpinar E.F., Alan Y., Kursat M. 2020. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal, 27 (1): 160-170.
  • Nikolić, M., Stevović S. 2015. Family Asteraceae as a sustainable planning tool in phytoremediation and its relevance in urban areas. Urban Forestry and Urban Greening, 14 (4): 782-789.
  • Tahtiharju S., Rijpkema A.S., Vetterli A., Albert V.A., Teeri T.H., Elomaa P. 2012. Evolution and diversification of the CYC/TB1 gene family in Asteraceae a comparative study in Gerbera (Mutisieae) and sunflower (Heliantheae). Molecular Biology and Evolution, 29 (4): 1155-1166.
  • Panda S.K., Luyten W. 2018. Antiparasitic activity in Asteraceae with special attention to ethnobotanical use by the tribes of Odisha, India. (Review). Parasite, 25, 10.
  • Kaffash S., Sefidkon F., Mafakheri S. 2020. Comparing Essential Oil Composition of Cultivated and Wild Samples of Achillea biebersteinii Afan. in Kurdistan Province. Journal of Medicinal plants and By-product, 9 (2): 149-158.
  • Başer K.H.C. 2016. Essential oils of Achillea species of Turkey. Natural Volatiles and Essential Oils, 3 (1): 1-14.
  • Qader K.O., Al-Saadi S.A., Faraj I.M. 2018. Phytochemical Constituents of Leaves Essential oils of Achillea fragrantissima (Asteraceae) from Iraq. Aro-the Scientific Journal of Koya University, 6 (2): 56-62.
  • Mottaghi M. P., Salehi Shanjani Jafari A.A., Mirza M., Bihamta M.R. 2016. Eessential Oil Composition of Achillea filipendulina, A. arabica and A. eriophora Cultivated under Temperate Climate in Iran. Journal of Medicinal plants and By-product, 5(2): 153-158.
  • Gulcin I. 2006. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology, 217 (2-3): 213-20.
  • Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activity of products of browning reaction. The Japanese journal of nutrition and dietetics, 44(6): 307-315.
  • Blois M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181 (4617): 1199-1200.
  • Wu L.C., Chang L.H., Chen S. H., Fan N. C., Ho J.A. 2009. Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. LWT-Food Science and Technology, 42 (9): 1513-1519.
  • Siddall T.L., Ouse D.G., Benko Z.L., Garvin G.M., Jackson J.L., McQuiston J.M., Ricks M.J., Thibault T.D., Turner J.A., VanHeertum J.C., Weimer M.R. 2002. Synthesis and herbicidal activity of phenyl‐substituted benzoylpyrazoles. Pest Management Science: formerly Pesticide Science, 58 (12): 1175-1186.
  • Seal T. 2016. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. Journal of Applied Pharmaceutical Science, 6 (2): 157-166.
  • Ceylan H., Budak H., Kocpinar E.F., Baltaci N.G., Erdogan O. 2019. Examining the link between dose-dependent dietary iron intake and Alzheimer’s disease through oxidative stress in the rat cortex. Journal of Trace Elements in Medicine and Biology, 56: 198-206.
  • Kocpınar E.F., Kursat M., Savci A., Alan Y. 2020. Some biological properties of ethanol extract prepared from the aerial parts of Scutellaria albida subsp candensest. Bitlis Eren University Journal of Science and Technology, 10 (2): 43-48.
  • Bankole O., Olasunkanmi A. 2019. In Vitro Antioxidant Activity of Aqeous Leaf Extract of Delonix regia. Journal of Science and Technology,. 5 (2): 71-77.
  • Hamrouni-Sellami I., Rahali F.Z., Rebey I.B., Bourgou S., Limam F., and Marzouk B. 2013. Total phenolics, flavonoids, and antioxidant activity of sage (Salvia officinalis L.) plants as affected by different drying methods. Food and Bioprocess Technology, 6 (3): 806-817.
  • Mohan S.C., Anand T., 2019. In vitro Antioxidant Activity of Leaf and Bark Extracts of Barringtonia acutangula Linn. International Research Journal Biology Sci, 1 (1): 37-40.
  • Savcı A., Koçpınar E.F., Alan Y., Kurşat M., 2020. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal 27 (1): 160-170.
  • Gjorgieva D., Kadifkova T. Panovska T., Ruskovska Bačeva K., Stafilov T. 2013. Influence of heavy metal stress on antioxidant status and DNA damage in Urtica dioica. BioMed Research International, 1-6.

Association of the phenolic content, DNA protective activity and some antioxidant properties in the Achillea arabica Kotschy Flower

Year 2021, Volume: 10 Issue: 3, 773 - 783, 17.09.2021
https://doi.org/10.17798/bitlisfen.904487

Abstract

Farmakolojik şifalı bitkiler tarihteki ilk ilaçlarımızdır ve vücudumuza çay, baharat veya çiğ tüketerek farklı şekillerde alırız. Günümüzde daha etkili sentetik ilaçların yan etkileri nedeniyle farmakolojik bitkilerin kullanımına olan eğilim giderek artarak özellikle rutin hastalıkların tedavisinde ve bunlardan korunmada bitkilerden faydalanılmaktadır. Günlük yaşamımızda stres ve olumsuz çevresel koşullar, hücrede reaktif oksijen türlerinin konsantrasyonunu artırarak kanser ve Parkinson ve Alzheimer gibi çeşitli nörodejeneratif hastalıklarla ilişkili olduğu bilinen oksidatif strese neden olabilir. Antioksidanlar, reaktif oksijen türlerinin konsantrasyonunu düzenleyerek hücreyi hastalıklara karşı korur ve antioksidan olarak bilinen bitkisel fenolikler reaktif oksijen türlerinin süpürülmesi için güçlü adaylardır. Ayrıca bitki ve etken madde ile ilgili çalışmalar, Covid-19 salgını döneminde aşı geliştirme çalışmaları için önemli veriler sağladıkları için daha da önem kazanmıştır. Bu çalışma ile ileri araştırmalarda kullanılabilecek faydalı verilerin elde edilmesi amaçlanmıştır. Bu amaçla çeşitli farmakolojik özellikleri bilinen Asteraceae familyasının önemli üyelerinden biri olan Achillea arabica, Ağrı / Patnos'tan toplanmış ve herbaryum materyaline dönüştürülerek depolandı. Herbaryum bitkisinin çiçeklerinden DMSO ekstresi hazırlandı ve hazırlanan ekstrelerin, bazı in vitro antioksidan özellikleri, DNA koruyucu aktivitesi, ve HPLC ile 16 farklı fenolik içeriği kantitatif olarak belirlendi ve sonuçlar ilişkilendirilmeye çalışıldı.

Project Number

This work was not carried out under any project.

References

  • Alan Y., Savcı A., Koçpınar E.F., Kurşat M., Topdemir S., Karataş M., Çakmak B. 2019. Antimicrobial, antioxidant and DNA protective effects and phenolic content of Lallementia canescens (L.) Fisch. and CA Mey. and Lallementia peltata (L.) Fisch. and CA Mey. Biological Diversity and Conversation, 12 (3): 78-88.
  • Kikowska M., Dworacka M., Kędziora I., Thiem B. 2016. Eryngium creticum-ethnopharmacology, phytochemistry and pharmacological activity. A review. Revista Brasileira de Farmacognosia, 26 (3): 392-399.
  • Koc S., Isgor B.S., Isgor Y.G., Moghaddam N.S., Yildirim O. 2015. The potential medicinal value of plants from Asteraceae family with antioxidant defense enzymes as biological targets. Pharmaceutical Biology, 53 (5): 746-751.
  • Soumia B. 2018. Eryngium campestre L.: Polyphenolic and flavonoid compounds; Applications to health and disease. Polyphenols: Mechanisms of Action in Human Health and Disease Academic Press, (Second Edition): 69-79.
  • Nebija F., Stefkov G., Karapandzova M., Stafilov T., Panovska T.K., Kulevanova S. 2009. Chemical characterization and antioxidant activity of Eryngium campestre L., Apiaceae from Kosovo. Macedonian Pharmaceutical Society, Marshal Tito 13b/8, Skopje Macedonia, 23.
  • Savci A., Kocpinar E.F., Budak H., Ciftci M., Sisecioglu M. 2020. The Effects of Amoxicillin, Cefazolin, and Gentamicin Antibiotics on the Antioxidant System in Mouse Heart Tissues. Protein Peptide Letters, 27 (7): 614-622.
  • Budak H., Kocpinar E.F., Gonul N., Ceylan H., Erol H.S., Erdogan O. 2014. Stimulation of gene expression and activity of antioxidant related enzyme in Sprague Dawley rat kidney induced by long-term iron toxicity. Comperative Biochemistry Physiology C Toxicology and Pharmacology, 166: 44-50.
  • Budak H., Gonul N., Ceylan H., Kocpinar E.F. 2014. Impact of long term Fe3+ toxicity on expression of glutathione system in rat liver. Environmental Toxicology and Pharmacology, 37 (1): 365-70.
  • Savci A., Kocpinar E.F., Alan Y., Kursat M. 2020. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal, 27 (1): 160-170.
  • Nikolić, M., Stevović S. 2015. Family Asteraceae as a sustainable planning tool in phytoremediation and its relevance in urban areas. Urban Forestry and Urban Greening, 14 (4): 782-789.
  • Tahtiharju S., Rijpkema A.S., Vetterli A., Albert V.A., Teeri T.H., Elomaa P. 2012. Evolution and diversification of the CYC/TB1 gene family in Asteraceae a comparative study in Gerbera (Mutisieae) and sunflower (Heliantheae). Molecular Biology and Evolution, 29 (4): 1155-1166.
  • Panda S.K., Luyten W. 2018. Antiparasitic activity in Asteraceae with special attention to ethnobotanical use by the tribes of Odisha, India. (Review). Parasite, 25, 10.
  • Kaffash S., Sefidkon F., Mafakheri S. 2020. Comparing Essential Oil Composition of Cultivated and Wild Samples of Achillea biebersteinii Afan. in Kurdistan Province. Journal of Medicinal plants and By-product, 9 (2): 149-158.
  • Başer K.H.C. 2016. Essential oils of Achillea species of Turkey. Natural Volatiles and Essential Oils, 3 (1): 1-14.
  • Qader K.O., Al-Saadi S.A., Faraj I.M. 2018. Phytochemical Constituents of Leaves Essential oils of Achillea fragrantissima (Asteraceae) from Iraq. Aro-the Scientific Journal of Koya University, 6 (2): 56-62.
  • Mottaghi M. P., Salehi Shanjani Jafari A.A., Mirza M., Bihamta M.R. 2016. Eessential Oil Composition of Achillea filipendulina, A. arabica and A. eriophora Cultivated under Temperate Climate in Iran. Journal of Medicinal plants and By-product, 5(2): 153-158.
  • Gulcin I. 2006. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology, 217 (2-3): 213-20.
  • Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activity of products of browning reaction. The Japanese journal of nutrition and dietetics, 44(6): 307-315.
  • Blois M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181 (4617): 1199-1200.
  • Wu L.C., Chang L.H., Chen S. H., Fan N. C., Ho J.A. 2009. Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. LWT-Food Science and Technology, 42 (9): 1513-1519.
  • Siddall T.L., Ouse D.G., Benko Z.L., Garvin G.M., Jackson J.L., McQuiston J.M., Ricks M.J., Thibault T.D., Turner J.A., VanHeertum J.C., Weimer M.R. 2002. Synthesis and herbicidal activity of phenyl‐substituted benzoylpyrazoles. Pest Management Science: formerly Pesticide Science, 58 (12): 1175-1186.
  • Seal T. 2016. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. Journal of Applied Pharmaceutical Science, 6 (2): 157-166.
  • Ceylan H., Budak H., Kocpinar E.F., Baltaci N.G., Erdogan O. 2019. Examining the link between dose-dependent dietary iron intake and Alzheimer’s disease through oxidative stress in the rat cortex. Journal of Trace Elements in Medicine and Biology, 56: 198-206.
  • Kocpınar E.F., Kursat M., Savci A., Alan Y. 2020. Some biological properties of ethanol extract prepared from the aerial parts of Scutellaria albida subsp candensest. Bitlis Eren University Journal of Science and Technology, 10 (2): 43-48.
  • Bankole O., Olasunkanmi A. 2019. In Vitro Antioxidant Activity of Aqeous Leaf Extract of Delonix regia. Journal of Science and Technology,. 5 (2): 71-77.
  • Hamrouni-Sellami I., Rahali F.Z., Rebey I.B., Bourgou S., Limam F., and Marzouk B. 2013. Total phenolics, flavonoids, and antioxidant activity of sage (Salvia officinalis L.) plants as affected by different drying methods. Food and Bioprocess Technology, 6 (3): 806-817.
  • Mohan S.C., Anand T., 2019. In vitro Antioxidant Activity of Leaf and Bark Extracts of Barringtonia acutangula Linn. International Research Journal Biology Sci, 1 (1): 37-40.
  • Savcı A., Koçpınar E.F., Alan Y., Kurşat M., 2020. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal 27 (1): 160-170.
  • Gjorgieva D., Kadifkova T. Panovska T., Ruskovska Bačeva K., Stafilov T. 2013. Influence of heavy metal stress on antioxidant status and DNA damage in Urtica dioica. BioMed Research International, 1-6.
There are 29 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Araştırma Makalesi
Authors

Enver Fehim Koçpınar 0000-0002-6031-4664

Project Number This work was not carried out under any project.
Publication Date September 17, 2021
Submission Date March 28, 2021
Acceptance Date August 12, 2021
Published in Issue Year 2021 Volume: 10 Issue: 3

Cite

IEEE E. F. Koçpınar, “Association of the phenolic content, DNA protective activity and some antioxidant properties in the Achillea arabica Kotschy Flower”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 10, no. 3, pp. 773–783, 2021, doi: 10.17798/bitlisfen.904487.

Bitlis Eren University
Journal of Science Editor
Bitlis Eren University Graduate Institute
Bes Minare Mah. Ahmet Eren Bulvari, Merkez Kampus, 13000 BITLIS