Eritropoez için Yeni Bir Parametre: Kordon Kanında Immatür Retikülosit Fraksiyon Değerlerinin Klinik Faktörlerle İlişkisi ve Yenidoğan için Referans Değerleri
Abstract
Amaç: Olgunlaşmamış retikülosit fraksiyonu (immature reticulocyte fraction, IRF), retikülosit olgunluk indeksini gösteren yeni bir parametredir ve yenidoğanlarda eritropoietik aktiviteyi daha iyi değerlendirmek için yararlı olabilecek bağımsız bir eritropoez parametresini temsil eder. Bu çalışmada yenidoğanlarda IRF değerleri ile klinik özellikler arasındaki ilişki araştırılmış ve referans değerler elde edilmiştir.
Gereç ve Yöntemler: Bu prospektif çalışmaya 28-40 gebelik haftaları arasında olan yenidoğanlar dahil edildi. Doğumda, tam kan sayımı (complete blood count, CBC), kan gazları ve çeşitli biyokimyasal parametrelerin plazma konsantrasyonlarının ölçümleri için maternal venöz ve umbilikal kord kan örnekleri alındı.
Bulgular: Çalışmaya 99 term ve 24 preterm olmak üzere toplam 123 yenidoğan dahil edildi. Prematüre ve term bebeklerin gebelik haftalarına göre laboratuvar özellikleri karşılaştırıldığında, kordon kanı ortanca IRF değeri term bebeklerde prematüre bebeklere göre daha yüksek bulunurken (p=0.039), diğer laboratuvar bulguları anlamlı farklılık göstermedi. Ortanca IRF değeri term bebeklerde 0,52 (aralık, 0,15-1,00) ve prematüre bebeklerde 0,34 (aralık, 0,16-0,76) idi. Term ve preterm yenidoğanlar için kordon kanında IRF referans değerleri belirlendi. IRF düzeyleri ile hem RDW (r=0,423, p<0,001) hem de CRP (r=0,389, p<0,001) düzeyleri arasında orta düzeyde pozitif korelasyonlar gözlendi.
Sonuç: Bu çalışmanın sonuçları, yenidoğanların IRF değerlerinin maternal değişkenlerden etkilenmediğini ve doğum haftası ile değiştiğini göstermiştir. Bu sonuçlar yenidoğanlarda IRF değerini kullanarak yapılacak olan sonraki çalışmalar için bir rehber olarak kabul edilebilir.
Supporting Institution
Kırıkkale Üniversitesi-BAP Koordinasyon Birimi
Project Number
2011/48
References
- Qaiser DH, Sandila MP, Ahmed ST, Kazmi T. Haematological reference values for full-term, healthy, newborns of Karachi, Pakistan. J Pak Med Assoc. 2009;59(9):618-22.
- Abdurrahman MB, Adekoje MA. Haematological values in Northern Nigerian neonates. Trans R Soc Trop Med Hyg. 1983;77(6):786-8.
- Maconi M, Formisano D, Cavalca L, Rolfo A, Cardaropoli S, Danise P. Reticulocyte count and reticulocyte maturation profile in human umbilical cord blood from healthy newborns. Lab Hematol. 2010;16(1):3-7.
- Ianni B, McDaniel H, Savilo E, Wade C, Micetic B, Johnson S, et al. Defining normal healthy term newborn automated hematologic reference intervals at 24 hours of life. Arch Pathol Lab Med. 2021;145(1):66-74.
- Makela E, Takala TI, Suominen P, Matomaki J, Salmi TT, Rajamaki A, et al. Hematological parameters in preterm infants from birth to 16 weeks of age with reference to iron balance. Clin Chem Lab Med. 2008;46(4):551-7.
- Schiza V, Giapros V, Pantou K, Theocharis P, Challa A, Andronikou S. Serum transferrin receptor, ferritin, and reticulocyte maturity indices during the first year of life in ‘large’ preterm infants. Eur J Haematol. 2007;79(5):439-46.
- Ringoringo HP, Purnamasari L, Yunanto A, Syahadatina M, Hidayah N. Reference range of complete blood count, Ret-He, immature reticulocyte fraction, reticulocyte production index in healthy babies aged 1-4 months. Sci Rep. 2023;13(1):423.
- Christensen RD, Henry E, Bennett ST, Yaish HM. Reference intervals for reticulocyte parameters of infants during their first 90 days after birth. J Perinatol. 2016;36(1):61-6.
- James DK, Steer PJ, Weiner CP, Gonik B. Prolonged pregnancy. In: James DK, Steer PJ, Weiner CP, editors. High risk pregnancy: management options. 3rd ed. Elsevier Saunders; 2005. p.1376-82.
- Ferber A, Fridel Z, Weissmann-Brenner A, Minior VK, Divon MY. Are elevated fetal nucleated red blood cell counts an indirect reflection of enhanced erythropoietin activity? Am J Obstet Gynecol. 2004;190(5):1473-5.
- Ferber A, Grassi A, Akyol D, O'Reilly-Green C, Divon MY. The association of fetal heart rate patterns with nucleated red blood cell counts at birth. Am J Obstet Gynecol. 2003;188(5):1228-30.
- Türkmen D, Özsoylu S, Akyıldız BN. Comparison of the value of immature retyculocyte and immature platelet in the diagnosis of sepsis. Pediatr Int. 2022;64(1):e14882.
- Butthep P, Wisedpanichkij R, Jindadamrongwech S, Fucharoen S. Elevated erythropoietin and cytokines levels are related to impaired reticulocyte maturation in thalassemic patients. Blood Cells Mol Dis. 2015;54(2):170-6.
- Warwood TL, Ohls RK, Wiedmeier SE, Lambert DK, Jones C, Scoffield SH, et al. Single-dose darbepoetin administration to anemic preterm neonates. J Perinatol. 2005;25(11):725-30.
- Ervasti M, Kotisaari S, Sankilampi U, Heinonen S, Punnonen K. The relationship between red blood cell and reticulocyte indices and serum markers of iron status in the cord blood of newborns. Clin Chem Lab Med. 2007;45(8):1000-3.
A New Parameter for Erythropoiesis: The Relationship of Immature Reticulocyte Fraction Values in Cord Blood with Clinical Factors and Reference Values for Newborns
Abstract
Aim: The immature reticulocyte fraction (IRF) is a new parameter for the reticulocyte maturity index, representing an independent parameter of erythropoiesis that may be useful to better assess erythropoietic activity in neonates. In this study, the relationship between IRF values and clinical features in newborns was investigated and reference values were obtained.
Material and Methods: Newborns between 28-40 weeks of gestation were included in this prospective study. At birth, maternal venous and cord blood samples were obtained for measurements of complete blood count (CBC), blood gases, and plasma concentrations of various biochemical parameters.
Results: A total of 123 newborns, 99 term and 24 preterm, were included in the study. When the laboratory characteristics of the premature and term babies were compared according to their gestational weeks, while the median IRF value of cord blood was higher in term babies than in premature babies (p=0.039), other laboratory findings did not differ significantly. The median IRF value was 0.52 (range, 0.15-1.00) in term infants and 0.34 (range, 0.16-0.76) in preterm infants. IRF reference values for the term and preterm newborns were determined in cord blood. Moderately positive correlations were observed between the IRF levels and both the RDW (r=0.423, p<0.001) and the CRP (r=0.389, p<0.001) levels.
Conclusion: The results of this study showed that newborns' IRF values were not affected by maternal variables and changed with the week of birth. The results of this study might be considered a guide for future studies using IRF value in newborns.
Project Number
2011/48
References
- Qaiser DH, Sandila MP, Ahmed ST, Kazmi T. Haematological reference values for full-term, healthy, newborns of Karachi, Pakistan. J Pak Med Assoc. 2009;59(9):618-22.
- Abdurrahman MB, Adekoje MA. Haematological values in Northern Nigerian neonates. Trans R Soc Trop Med Hyg. 1983;77(6):786-8.
- Maconi M, Formisano D, Cavalca L, Rolfo A, Cardaropoli S, Danise P. Reticulocyte count and reticulocyte maturation profile in human umbilical cord blood from healthy newborns. Lab Hematol. 2010;16(1):3-7.
- Ianni B, McDaniel H, Savilo E, Wade C, Micetic B, Johnson S, et al. Defining normal healthy term newborn automated hematologic reference intervals at 24 hours of life. Arch Pathol Lab Med. 2021;145(1):66-74.
- Makela E, Takala TI, Suominen P, Matomaki J, Salmi TT, Rajamaki A, et al. Hematological parameters in preterm infants from birth to 16 weeks of age with reference to iron balance. Clin Chem Lab Med. 2008;46(4):551-7.
- Schiza V, Giapros V, Pantou K, Theocharis P, Challa A, Andronikou S. Serum transferrin receptor, ferritin, and reticulocyte maturity indices during the first year of life in ‘large’ preterm infants. Eur J Haematol. 2007;79(5):439-46.
- Ringoringo HP, Purnamasari L, Yunanto A, Syahadatina M, Hidayah N. Reference range of complete blood count, Ret-He, immature reticulocyte fraction, reticulocyte production index in healthy babies aged 1-4 months. Sci Rep. 2023;13(1):423.
- Christensen RD, Henry E, Bennett ST, Yaish HM. Reference intervals for reticulocyte parameters of infants during their first 90 days after birth. J Perinatol. 2016;36(1):61-6.
- James DK, Steer PJ, Weiner CP, Gonik B. Prolonged pregnancy. In: James DK, Steer PJ, Weiner CP, editors. High risk pregnancy: management options. 3rd ed. Elsevier Saunders; 2005. p.1376-82.
- Ferber A, Fridel Z, Weissmann-Brenner A, Minior VK, Divon MY. Are elevated fetal nucleated red blood cell counts an indirect reflection of enhanced erythropoietin activity? Am J Obstet Gynecol. 2004;190(5):1473-5.
- Ferber A, Grassi A, Akyol D, O'Reilly-Green C, Divon MY. The association of fetal heart rate patterns with nucleated red blood cell counts at birth. Am J Obstet Gynecol. 2003;188(5):1228-30.
- Türkmen D, Özsoylu S, Akyıldız BN. Comparison of the value of immature retyculocyte and immature platelet in the diagnosis of sepsis. Pediatr Int. 2022;64(1):e14882.
- Butthep P, Wisedpanichkij R, Jindadamrongwech S, Fucharoen S. Elevated erythropoietin and cytokines levels are related to impaired reticulocyte maturation in thalassemic patients. Blood Cells Mol Dis. 2015;54(2):170-6.
- Warwood TL, Ohls RK, Wiedmeier SE, Lambert DK, Jones C, Scoffield SH, et al. Single-dose darbepoetin administration to anemic preterm neonates. J Perinatol. 2005;25(11):725-30.
- Ervasti M, Kotisaari S, Sankilampi U, Heinonen S, Punnonen K. The relationship between red blood cell and reticulocyte indices and serum markers of iron status in the cord blood of newborns. Clin Chem Lab Med. 2007;45(8):1000-3.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Article |
| Authors | |
| Project Number | 2011/48 |
| Publication Date | April 30, 2023 |
| Submission Date | September 8, 2022 |
| Published in Issue | Year 2023 Volume: 25 Issue: 1 |
Cite
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