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Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği

Year 2018, Volume: 11 Issue: 2, 77 - 82, 31.12.2018

Abstract

Gebelik ile ilişkili glikoproteinler (PAGs)
koyunları da kapsayan ruminantlarda gebelikte trofoblastik hücrelerden üretilmektedir.
Sunulan bu çalışmada Konya Merinosu koyunlarda erken gebelik ve tüm gebelik
boyunca kan plazmasındaki PAGs konsantrasyonunun belirlenmesi amaçlandı. Toplam
16 baş gebe koyun kullanıldı. Çalışmanın birinci aşamasında (gebelik boyunca
PAGs profilinin izlenmesi) 8 baş koyundan gebeliğin 0, 55, 85, 113 ve 145.
günlerinde kan plazması toplandı. İkinci aşamada (erken gebelikteki PAGs
profilinin belirlenmesi) ise gebeliğin 18, 21, 25, 28 ve 35. günlerinde kan
plazma örnekleri toplandı. Elde edilen plazma örneklerinde PAGs değerleri
ticari (Sheep Pregnancy Associated Glycoproteins) ELISA kiti ile ölçüldü.
Sonuçlar değerlendirildiğinde gebeliğin 0. gününde plazmada bazal seviyede PAGs
olduğu tespit edilmiştir (14,99±1,63 ng/ml). Birinci aşamadaki veriler;
gebeliğin 0. günü ile kıyaslandığında; gebeliğin 55, 85, 113 ve 145.
günlerindeki değerleri arasında istatistikî olarak önemli bir fark tespit
edilmiştir (p<0,05). İkinci aşamada ise gebeliğin 35. gününde plazma PAGs
değerinin 18. gün ile kıyaslandığında önemli ölçüde arttığı tespit edilmiştir
(p<0,05). Elde edilen sonuçlar değerlendiğinde Konya Merinosu koyunlarda
gebelik boyunca plazma PAGs düzeyinin sürekli artan bir profil çizdiği
görülmektedir. Yine bu sonuçlara göre çalışmada kullanılan PAGs kiti ile Konya
Merinosu koyunlarda en erken 35. günde gebelik teşhisi yapılabileceği
söylenebilir.

References

  • 1. Spencer TE, Burghardt RC, Johnson GA, Bazer FW. (2004). Conceptus Signals for Establishment and Maintenance of Pregnancy. Anim Reprod Sci. 82-83, 537-550.
  • 2. Bazer FW, Guoyao W, Spencer TE et al. (2010). Novel Pathways for Implantation and Establishment and Maintenance of Pregnancy in Mammals. Molecular Human Reproduction. 16 (3): 135-152.
  • 3. Bazer FW, Wu G, Johnson GA, Kim J, Song G. (2011). Uterine Histotroph and Conceptus Development: Select Nutrients and Secreted Phosphoprotein 1 Affect Mechanistic Target of Rapamycin Cell Signaling in Ewes. Biology of Reproduction. 85: 1094-1107.
  • 4. Spencer TE, Johnson GA, Bazer FW, Burghardt RC. (2004). Focus on Implantation, Implantation Mechanisms. Insights from the Sheep Reproduction. 128: 657-668.
  • 5. Igwebuike UM. (2009) A review of uterine structural modifications that influence conceptus implantation and development in sheep and goats. Animal Reproduction Science. 112(1-2): 1-7.
  • 6. Wallace RM, Pohler KG, Smith MF, Green JA. (2015). Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy. Reproduction. 149: 115-126.
  • 7. Igwebuike UM. (2006). Trophoblast cells of ruminant placentas-A minireview. Animal Reproduction Science. 93: 185-198.
  • 8. Szafranska B, Panasiewicz G, Majewska M. (2006). Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia)-a review. Reprod Nutr Dev. 5: 481-502.
  • 9. Haugejorden G, Waage S, Dahl E et al. (2006). Pregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring. Theriogenology. 66: 1976-1984.
  • 10. Ranilla MJ, Sulon J, Carro MD, Mantecon AR, Beckers JF. (1994). Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during gestation in Churra and Merino sheep. Theriogenology. 42: 537-545.
  • 11. Karen A, Beckers JF, Sulon J et al. (2003). Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests. Theriogenology. 59 (9):1941-8.
  • 12. Ranilla MJ, Sulon J, Mantecon AR, Beckers JF, Carro MD. (1997). Plasma pregnancy-associated glycoprotein and progesterone concentrations in pregnant Assaf ewes carrying single and twin lambs. Small Rumin Res. 24: 125-131.
  • 13. González F, Sulon J, Garbayo JM et al. (1999). Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples. Theriogenology. 52: 717-725.
  • 14. González F, Sulon J, Garbayo JM et al. (2000). Secretory profiles of pregnancy-associated glycoproteins at different stages of pregnancy in the goat. Reprod Domest Anim. 35: 79-82.
  • 15. Roberts JN, May KJ, Veiga-Lopez A. (2017). Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep. Theriogenology. 89: 271–279.
  • 16. Moussafir Z, Allai L, El Khalil K, Essamadi A, El Amiri B. (2018). Could a bovine pregnancy rapid test be an alternative to a commercial pregnancy-associated glycoprotein ELISA test in dairy cattle? Anim Reprod Sci. 192: 78-83.
  • 17. Reyhaneh H, Holm, Stefan B et al. (2018). Pregnancy-associated glycoproteins in cows with retained fetal membra-nes. Theriogenology. 105: 158-163.
  • 18. Reese ST, Pereira MHC, Edwards JL, Vasconcelos JLM, Pohler KG. (2018). Pregnancy diagnosis in cattle using pregnancy as-sociated glycoprotein concentration in circulation at day 24 of gestation. Theriogenology. 106: 178-185.
  • 19. Lucy M, Green J, Poock S. Pregnancy determination in cattle: A review of available alternatives. Proceedings Applied Repro-ductive Strategies in Beef Cattle. Erişim: http://www.appliedreprostrategies.com/2011/joplin/proceedings/27lucy.pdf. Erişim Tarihi: 04.09.2014
  • 20. Balhara AK, Gupta M, Singh S, Mohanty AK, Singh I. (2013). Early Pregnancy Diagnosis in Bovines: Current Status and Futu-re Directions. http://dx.doi.org/10.1155/2013/958540. Erişim Tarihi: 04.09.2014
  • 21. de Miranda E Silva Chaves C, Dias da Costa RL, Roncato Duarte KM et al. (2017). Visual ELISA for detection of pregnancy-associated glycoproteins (PAGs) in ewe serum. Theriogeno-logy. 15(97): 78-82.
  • 22. Gajewski Z, Melo de Sousa N, Beckers JF et al. (2008). Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows. J Physiol Pharmacol. 9:55-64.
  • 23. Ledezma-Torres RA, Beckers JF, Holtz W. (2006). Assessment of plasma profile of pregnancy associated glycoprotein (PAG) in sheep with a heterologous (anti-caPAG (55 + 59) RIA and its potential for diagnosing pregnancy. Theriogenology. 66: 906–912.
  • 24. Lawrence PR, DALE AR. (1992). Growth and Microvascular Development of the Uterus during Early Pregnancy in Ewes. Biology of Reproduction. 47: 698-708.
  • 25. Bairagi S, Quinn KE, Crane AR et al. (2016). Maternal environment and placental vascularization in small ruminants. Theriogenology 86: 288-305.
  • 26. El Amiri B, Sousa NM, Alvarez Oxiley A, Hadarbach D, Beckers JF. (2015). Pregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep. Res Vet Sci. 99: 30-36.
  • 27. Rovani MT, Cezar AS, Rigo ML et al. (2016). Evaluation of a bovine pregnancy-associated glycoprotein enzyme-linked immunosorbent assay kit for serological diagnosis of pregnancy in sheep. Ciência Rural. 46(2): 362-367.
  • 28. El Amiri B, Karen A, Sulon J et al. (2007). Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep, Reproduction in Domestic Animals. 42: 257-262.
  • 29. Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF. (1992). Radioimmunoassay of a Bovine Pregnancy-Associated Glycoprotein in Serum: Its Application for Pregnancy Diagnosis. Biology of Reproduction. 46: 83-92.
  • 30. Vandaele L, Verberckmoes S, El Amiri B et al. (2005). Use of a homologous radioimmunoassay (RIA) to evaluate the effect of maternal and foetal parameters on pregnancy-associated glycoproteins (PAGs) concentrations in sheep. Theriogenology. 63(7): 1914-1924.
Year 2018, Volume: 11 Issue: 2, 77 - 82, 31.12.2018

Abstract

References

  • 1. Spencer TE, Burghardt RC, Johnson GA, Bazer FW. (2004). Conceptus Signals for Establishment and Maintenance of Pregnancy. Anim Reprod Sci. 82-83, 537-550.
  • 2. Bazer FW, Guoyao W, Spencer TE et al. (2010). Novel Pathways for Implantation and Establishment and Maintenance of Pregnancy in Mammals. Molecular Human Reproduction. 16 (3): 135-152.
  • 3. Bazer FW, Wu G, Johnson GA, Kim J, Song G. (2011). Uterine Histotroph and Conceptus Development: Select Nutrients and Secreted Phosphoprotein 1 Affect Mechanistic Target of Rapamycin Cell Signaling in Ewes. Biology of Reproduction. 85: 1094-1107.
  • 4. Spencer TE, Johnson GA, Bazer FW, Burghardt RC. (2004). Focus on Implantation, Implantation Mechanisms. Insights from the Sheep Reproduction. 128: 657-668.
  • 5. Igwebuike UM. (2009) A review of uterine structural modifications that influence conceptus implantation and development in sheep and goats. Animal Reproduction Science. 112(1-2): 1-7.
  • 6. Wallace RM, Pohler KG, Smith MF, Green JA. (2015). Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy. Reproduction. 149: 115-126.
  • 7. Igwebuike UM. (2006). Trophoblast cells of ruminant placentas-A minireview. Animal Reproduction Science. 93: 185-198.
  • 8. Szafranska B, Panasiewicz G, Majewska M. (2006). Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia)-a review. Reprod Nutr Dev. 5: 481-502.
  • 9. Haugejorden G, Waage S, Dahl E et al. (2006). Pregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring. Theriogenology. 66: 1976-1984.
  • 10. Ranilla MJ, Sulon J, Carro MD, Mantecon AR, Beckers JF. (1994). Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during gestation in Churra and Merino sheep. Theriogenology. 42: 537-545.
  • 11. Karen A, Beckers JF, Sulon J et al. (2003). Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests. Theriogenology. 59 (9):1941-8.
  • 12. Ranilla MJ, Sulon J, Mantecon AR, Beckers JF, Carro MD. (1997). Plasma pregnancy-associated glycoprotein and progesterone concentrations in pregnant Assaf ewes carrying single and twin lambs. Small Rumin Res. 24: 125-131.
  • 13. González F, Sulon J, Garbayo JM et al. (1999). Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples. Theriogenology. 52: 717-725.
  • 14. González F, Sulon J, Garbayo JM et al. (2000). Secretory profiles of pregnancy-associated glycoproteins at different stages of pregnancy in the goat. Reprod Domest Anim. 35: 79-82.
  • 15. Roberts JN, May KJ, Veiga-Lopez A. (2017). Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep. Theriogenology. 89: 271–279.
  • 16. Moussafir Z, Allai L, El Khalil K, Essamadi A, El Amiri B. (2018). Could a bovine pregnancy rapid test be an alternative to a commercial pregnancy-associated glycoprotein ELISA test in dairy cattle? Anim Reprod Sci. 192: 78-83.
  • 17. Reyhaneh H, Holm, Stefan B et al. (2018). Pregnancy-associated glycoproteins in cows with retained fetal membra-nes. Theriogenology. 105: 158-163.
  • 18. Reese ST, Pereira MHC, Edwards JL, Vasconcelos JLM, Pohler KG. (2018). Pregnancy diagnosis in cattle using pregnancy as-sociated glycoprotein concentration in circulation at day 24 of gestation. Theriogenology. 106: 178-185.
  • 19. Lucy M, Green J, Poock S. Pregnancy determination in cattle: A review of available alternatives. Proceedings Applied Repro-ductive Strategies in Beef Cattle. Erişim: http://www.appliedreprostrategies.com/2011/joplin/proceedings/27lucy.pdf. Erişim Tarihi: 04.09.2014
  • 20. Balhara AK, Gupta M, Singh S, Mohanty AK, Singh I. (2013). Early Pregnancy Diagnosis in Bovines: Current Status and Futu-re Directions. http://dx.doi.org/10.1155/2013/958540. Erişim Tarihi: 04.09.2014
  • 21. de Miranda E Silva Chaves C, Dias da Costa RL, Roncato Duarte KM et al. (2017). Visual ELISA for detection of pregnancy-associated glycoproteins (PAGs) in ewe serum. Theriogeno-logy. 15(97): 78-82.
  • 22. Gajewski Z, Melo de Sousa N, Beckers JF et al. (2008). Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows. J Physiol Pharmacol. 9:55-64.
  • 23. Ledezma-Torres RA, Beckers JF, Holtz W. (2006). Assessment of plasma profile of pregnancy associated glycoprotein (PAG) in sheep with a heterologous (anti-caPAG (55 + 59) RIA and its potential for diagnosing pregnancy. Theriogenology. 66: 906–912.
  • 24. Lawrence PR, DALE AR. (1992). Growth and Microvascular Development of the Uterus during Early Pregnancy in Ewes. Biology of Reproduction. 47: 698-708.
  • 25. Bairagi S, Quinn KE, Crane AR et al. (2016). Maternal environment and placental vascularization in small ruminants. Theriogenology 86: 288-305.
  • 26. El Amiri B, Sousa NM, Alvarez Oxiley A, Hadarbach D, Beckers JF. (2015). Pregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep. Res Vet Sci. 99: 30-36.
  • 27. Rovani MT, Cezar AS, Rigo ML et al. (2016). Evaluation of a bovine pregnancy-associated glycoprotein enzyme-linked immunosorbent assay kit for serological diagnosis of pregnancy in sheep. Ciência Rural. 46(2): 362-367.
  • 28. El Amiri B, Karen A, Sulon J et al. (2007). Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep, Reproduction in Domestic Animals. 42: 257-262.
  • 29. Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF. (1992). Radioimmunoassay of a Bovine Pregnancy-Associated Glycoprotein in Serum: Its Application for Pregnancy Diagnosis. Biology of Reproduction. 46: 83-92.
  • 30. Vandaele L, Verberckmoes S, El Amiri B et al. (2005). Use of a homologous radioimmunoassay (RIA) to evaluate the effect of maternal and foetal parameters on pregnancy-associated glycoproteins (PAGs) concentrations in sheep. Theriogenology. 63(7): 1914-1924.
There are 30 citations in total.

Details

Primary Language Turkish
Journal Section Research
Authors

Uğur Uçar This is me

Mehmet Köse

Mehmet Osman Atlı

Publication Date December 31, 2018
Acceptance Date December 23, 2018
Published in Issue Year 2018 Volume: 11 Issue: 2

Cite

APA Uçar, U., Köse, M., & Atlı, M. O. (2018). Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 11(2), 77-82.
AMA Uçar U, Köse M, Atlı MO. Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği. Dicle Üniv Vet Fak Derg. December 2018;11(2):77-82.
Chicago Uçar, Uğur, Mehmet Köse, and Mehmet Osman Atlı. “Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili Ve Erken Gebelik Tanısında Kullanılabilirliği”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 11, no. 2 (December 2018): 77-82.
EndNote Uçar U, Köse M, Atlı MO (December 1, 2018) Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği. Dicle Üniversitesi Veteriner Fakültesi Dergisi 11 2 77–82.
IEEE U. Uçar, M. Köse, and M. O. Atlı, “Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği”, Dicle Üniv Vet Fak Derg, vol. 11, no. 2, pp. 77–82, 2018.
ISNAD Uçar, Uğur et al. “Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili Ve Erken Gebelik Tanısında Kullanılabilirliği”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 11/2 (December 2018), 77-82.
JAMA Uçar U, Köse M, Atlı MO. Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği. Dicle Üniv Vet Fak Derg. 2018;11:77–82.
MLA Uçar, Uğur et al. “Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili Ve Erken Gebelik Tanısında Kullanılabilirliği”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 11, no. 2, 2018, pp. 77-82.
Vancouver Uçar U, Köse M, Atlı MO. Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği. Dicle Üniv Vet Fak Derg. 2018;11(2):77-82.