Correlation of Adenosine Deaminase Activity and Somatic Cell Count in Dairy Cows with Subclinical Mastitis
Abstract
In this study, we aimed
to determine the levels of adenosine deaminase (ADA), which plays important
role in inflammatory process in sera and milk in cows with subclinical mastitis
and state the relationship between this enzyme with somatic cell count. Holstein
cows which was consisted the study group allocated into two groups subclinical
mastitis (n = 40) and healthy cows (n = 15).
After performing the California Mastitis Test (CMT), the cows resulted
suspicious; included the study group, the cows which showed CMT as negative
constitute the control group. Somatic cell counts were determined with flow
cytometry assay. In subclinical mastitis group, serum and milk ADA values were
8.11 and 3.38 IU / L, respectively. Somatic cell counts were 248x103 and 132x103
in subclinical mastitis and control groups, respectively (p <0.01). There
was a positive correlation between milk ADA and somatic cell count (p <0.01,
r = 0.611). As a result, adenosine deaminase can be used as a marker in the
diagnosis of subclinical mastitis in cows.
References
- 1. Kumar V, Sharma A. (2009). Adenosine: An Endogenous Modulator of Innate İmmune System with Therapeutic Poten-tial. Eur J Pharmacol. 15: 7-15.
- 2. Fredholm BB, Johansson S, Wang YW. (2011). Adenosine and the Regulation of Metabolism and Body Temperature Adv Pharmacol. 61: 77-94.
- 3. Antonioli L, Fornai M, Blandizzi C, Pacher P, Haskó G. (2019). Adenosine Signaling and the Immune System: When a lot Could be too Much. Immunol Lett. 205:9-15.
- 4. Fávero JF, Da Silva AS, Bottari NB, et al. (2018). Physiological Changes in the Adenosine Deaminase Activity, Antioxidant and Inflammatory Parameters in Pregnant Cows and at Postpar-tum. J Anim Physiol Anim Nutr. 102(4): 910-916.
- 5. Rodney EK, Yeung CY, Ingolia DE. (1985). Adenosine Deami-nase Deficiency and Severe Combined Immunodeficiencies. Trends Genet. 1: 278-283.
- 6. Baştan A. (2013). İneklerde Meme Sağlığı ve Sorunları Genişletilmiş II. Baskı. Kardelen Ofset Matbaacılık Tanıtım Hiz. Ankara, Türkiye.
- 7. Kossaibati MA, Esslemont RJ. (2007). The Costs of Production Diseases in Dairy Herds in England. Vet J. 154: 41-51.
- 8. Pyörälä S. (2003). Indicators of Inflammation in the Diagnosis of Mastitis. Vet Res. 34(5): 565-578.
- 9. Sharma N, Singh NK, Bhadwal MS. (2011). Relationship of Somatic Cell Count and Mastitis: An Overview. Asian-Australas J Anim Sci. 24 (3): 429-438.
- 10. Antanaitis R, Žilaitis V, Juozaitienė V, et al. (2015). Efficient Diagnostics and Treatment of Bovine Mastitis According to Herd Management Parameters. Veterinarija İr Zootechnika. 69 (91):3-10.
- 11. Alais C. (1974). Science du Lait. Principes des Techniques Laitières. Editeurs Boulevard, Paris.
- 12. Guisti G, Galanti B. (1984). Adenosine Deaminase: Colorimetric Method. Methods of Enzymatic Analysis. 5th edition. Bergmeyer HU (Ed). Pp. 315-323. Verlag Chemie Weinheim, Germany
- 13. Kehrli ME, Harp JA. (2001). Immunity in the Mammary Gland. Vet Clin North Am Food Anim Pract. 17(3): 495-516.
- 14. Pilla R, Malvisi M, Snel GGM, et al. (2013). Differential Cell Count as an Alternative Method to Diagnose Dairy Cow Masti-tis. J Dairy Sci. 96(3): 1653-1660.
- 15. Conlon BA, Law WR. (2004). Macrophages Are a Source of Extracellular Adenosine Deaminase 2 During Inflammatory Responses. Clin Exp Immunol. 138(1): 14-20.
- 16. Salmanoğlu B, Pamukçu T, Yarım G. (2002). Subklinik Mastitisli Süt İneklerinde Meme içi Levamizol Uygulanmasında Süt ve Kanda Adenozin Deaminaz, Vitamin A, B-Karotin Düzeyleri. Ankara Üniv Vet Fak Derg. 49: 17-21.
Abstract
Bu çalışmada yangısal
süreçte önemli görevleri olan adenozin deaminazın, subklinik mastitisli
ineklerde serum ve süt adenozin ademinaz (ADA) düzeylerinin belirlenmesi ve bu
enzimin somatik hücre sayısı ile ilişkisinin ortaya konması amaçlandı. Çalışma
grubunu oluşturan Holstein inekler; subklinik mastitisli (n=40) ve sağlıklı
inekler (n=15) olarak iki gruba ayrıldı. Kaliforniya mastitis testi (CMT)
sonucunda şüpheli sonuç veren ve herhangi bir klinik mastitis bulgusu
bulunmayan inekler subklinik mastitis grubunu, CMT negatif olan inekler ise
kontrol grubunu oluşturdu. Süt örneklerindeki somatik hücre sayıları flow
sitometri ile belirlendi. Subklinik
mastitis grubunda serum ve süt ADA değerleri sırasıyla 8,11 ve 3,38 IU/L
ölçüldü. Somatik hücre sayıları subklinik mastitis ve kontrol grubunda
sırasıyla 248x103 ve 132x103 bulundu (p<0.01). Süt ADA ile somatik hücre
sayısı arasında da pozitif korelasyon bulundu (p<0.01, r=0.611). Sonuç
olarak, adenozin deaminazın ineklerde subklinik mastitis olgularının tanısında
bir belirteç olarak kullanılabileceği kanısına varıldı.
References
- 1. Kumar V, Sharma A. (2009). Adenosine: An Endogenous Modulator of Innate İmmune System with Therapeutic Poten-tial. Eur J Pharmacol. 15: 7-15.
- 2. Fredholm BB, Johansson S, Wang YW. (2011). Adenosine and the Regulation of Metabolism and Body Temperature Adv Pharmacol. 61: 77-94.
- 3. Antonioli L, Fornai M, Blandizzi C, Pacher P, Haskó G. (2019). Adenosine Signaling and the Immune System: When a lot Could be too Much. Immunol Lett. 205:9-15.
- 4. Fávero JF, Da Silva AS, Bottari NB, et al. (2018). Physiological Changes in the Adenosine Deaminase Activity, Antioxidant and Inflammatory Parameters in Pregnant Cows and at Postpar-tum. J Anim Physiol Anim Nutr. 102(4): 910-916.
- 5. Rodney EK, Yeung CY, Ingolia DE. (1985). Adenosine Deami-nase Deficiency and Severe Combined Immunodeficiencies. Trends Genet. 1: 278-283.
- 6. Baştan A. (2013). İneklerde Meme Sağlığı ve Sorunları Genişletilmiş II. Baskı. Kardelen Ofset Matbaacılık Tanıtım Hiz. Ankara, Türkiye.
- 7. Kossaibati MA, Esslemont RJ. (2007). The Costs of Production Diseases in Dairy Herds in England. Vet J. 154: 41-51.
- 8. Pyörälä S. (2003). Indicators of Inflammation in the Diagnosis of Mastitis. Vet Res. 34(5): 565-578.
- 9. Sharma N, Singh NK, Bhadwal MS. (2011). Relationship of Somatic Cell Count and Mastitis: An Overview. Asian-Australas J Anim Sci. 24 (3): 429-438.
- 10. Antanaitis R, Žilaitis V, Juozaitienė V, et al. (2015). Efficient Diagnostics and Treatment of Bovine Mastitis According to Herd Management Parameters. Veterinarija İr Zootechnika. 69 (91):3-10.
- 11. Alais C. (1974). Science du Lait. Principes des Techniques Laitières. Editeurs Boulevard, Paris.
- 12. Guisti G, Galanti B. (1984). Adenosine Deaminase: Colorimetric Method. Methods of Enzymatic Analysis. 5th edition. Bergmeyer HU (Ed). Pp. 315-323. Verlag Chemie Weinheim, Germany
- 13. Kehrli ME, Harp JA. (2001). Immunity in the Mammary Gland. Vet Clin North Am Food Anim Pract. 17(3): 495-516.
- 14. Pilla R, Malvisi M, Snel GGM, et al. (2013). Differential Cell Count as an Alternative Method to Diagnose Dairy Cow Masti-tis. J Dairy Sci. 96(3): 1653-1660.
- 15. Conlon BA, Law WR. (2004). Macrophages Are a Source of Extracellular Adenosine Deaminase 2 During Inflammatory Responses. Clin Exp Immunol. 138(1): 14-20.
- 16. Salmanoğlu B, Pamukçu T, Yarım G. (2002). Subklinik Mastitisli Süt İneklerinde Meme içi Levamizol Uygulanmasında Süt ve Kanda Adenozin Deaminaz, Vitamin A, B-Karotin Düzeyleri. Ankara Üniv Vet Fak Derg. 49: 17-21.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research |
| Authors | |
| Publication Date | June 30, 2019 |
| Acceptance Date | June 25, 2019 |
| Published in Issue | Year 2019 Volume: 12 Issue: 1 |