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Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi

Year 2020, Volume: 13 Issue: 1, 24 - 32, 30.06.2020

Ayhan Atasever Elife Sayar Görkem Ekebaş Meryem Şentürk Meryem Eren

Abstract

Karbon tetraklorür (CCl4) ile kronik karaciğer hasarı oluşturulan ratlarda kabak çekirdeği yağının (KÇY) lezyonlar üzerine koruyucu etkisi, hücresel apoptozda kaspaz 3 aktivasyonu, serum Alanin Amino Transferaz (ALT) aktivitesi, trigliserit, total protein, albümin, total kolesterol düzeyleri ile karaciğer malondialdehit (MDA) ve total antioksidan kapasitesi (TAK) değerlendirildi. Çalışmada 48 adet rat, 12 hayvanlı 4 gruba ayrıldı. Grup I kontrol; Grup II ise CCl4 çalışma grubu olarak belirlendi. Grup III’e 4 ml/kg KÇY, 12 hafta gavajla verildi. Grup IV’e 4 hafta gavaj şeklinde KÇY verildi, takiben 8 hafta her gün 4 ml/kg KÇY ve intraperitoneal 0,2 ml/kg CCl4, haftada iki kere uygulandı. Grup III’de normal karaciğer dokusu gözlenirken; Grup II’de hepatositlerde yoğun makro ve mikroveziküler yağlanma, parankimde hücre infiltrasyonları, bağ doku artışına bağlı pseudolobulasyon formları görüldü. Grup IV’de oluşan lezyonların histopatolojisi Grup II ile benzerdi. Kontrol gruplarına göre çalışma gruplarındaki kaspaz 3 aktivitesi arttı. Grup II’de artan serum ALT aktivitesi (P<0,001), Grup IV’de değişiklik göstermedi. Grup II’de serum total protein ve albümin düzeyleri istatistiki önemde düşerken (P<0,001), Grup IV’de bu değerlerde sayısal azaldı. Grup IV’de yüksek olan serum total kolestrol düzeyleri KÇY’dan etkilenmedi. Grup II’de serum trigliserit düzeyleri önemli düzeyde artarken (P<0,001), Grup IV’de sayısal olarak düştü. Grup IV’de KÇY uygulanması, MDA değerlerini önemli düzeyde düşürürken (P<0,001), Total antioksidan kapasitesi yönünden ise gruplar arasında fark saptanamadı (P>0,05). Sonuç olarak; CCl4 ile birlikte KÇY verilen Grup IV’de karaciğer hasarı üzerinde bitkinin antioksidan özelliklerinin biyokimyasal veriler ve histopatolojik lezyonlar üzerine iyileştirici yönden anlamlı bir etkisi gözlenmedi.

Keywords

Histopatoloji İmmunhistokimya Karbon tetraklorür Kabak çekirdeği yağı Rat

Supporting Institution

Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiş ve yüksek lisans tezinden üretilmiştir.

Project Number

TYL-2018-8449

References

  • Referans1 Crawford JM (2003). Karaciğer ve safra yolları. (İçinde): Robbins Temel Patoloji. Robbins SL, Cotran RS, Kumar V (editörler). 7. Baskı, s. 591-630. Nobel Tıp Kitabevi, İstanbul, Türkiye.
  • Referans2 Recknagel RO, Glende EA Jr, Dolak JA, Waller RL. (1989). Mechanisms of carbon tetrachloride toxicity. Pharmacol Therapeut. 43: 139-154.
  • Referans3 Muriel P, Mourelle M. (1990). Prevention by silymarin of membrane alterations in acute CCl4 liver damage. J Appl Toxicol. 10: 275-279.
  • Referans4 Basu S (2003). Carbon tetrachloride-induced lipid peroxidation: Eicosanoid formation and their regulation by antioxidant nutrients. Toxicol. 189:113-127.
  • Referans5 Manibusan MK, Odin M, Eastmond DA. (2007). Postulated carbon tetrachloride mode of action: A review. J Environ Sci Heal C. 25: 185-209.
  • Referans6 Kalaycıoğlu L, Serpek B, Nizamlıoğlu M, Başpınar N, Tiftik AM (2000). Biyokimya. s. 450-496. Nobel Yayın Dağıtım Ltd. Şti, Ankara, Türkiye.
  • Referans7 Nkosi CZ, Opoku AR, Terblanche SE. (2006). Antioxidative effects of pumpkin seed (Cucurbita pepo) protein isolate in CCl4‐Induced liver injury in low‐protein fed rats. Phytotherap. Res. 20(11): 935-940.
  • Referans8 Etim OE, Akpan EJ, Usoh IF. (2008). Hepatotoxicity of carbon tetrachloride: Protective effect of Gongronema latifolium. J Pharm Sci. 21: 268-274.
  • Referans9 Gnanaprakash K, Madhusudhana CC, Ramkanth S, et al. (2010). Aqueous extract of Flacourtia indica prevents carbon tetrachloride induced hepatotoxicity in rat. Int J Biol Sci. 6: 51-55.
  • Referans10 Yehia HM, Al Olayan EM, Elkhadragy MF. (2013). Hepatoprotective role of the pomegranate (Punica granatum) juice on carbon tetrachloride induced oxidative stress in rats. Life Sci J. 10: 1534-1544.
  • Referans11 Zuinen R, Yamaji K, Aoki M, Chikuma T, Hojo H. (2007). Early induced, high-level interleukin-6 expression in the rat peritoneal cavity into which a hepatotoxicant carbon tetrachloride was administered. Toxicol Lett. 170: 42-48.
  • Referans12 Atasever A, Yaman D. (2014). The effects of grape seed and colchicine on carbon tetrachloride induced hepatic damage in rats. Exp Toxicol Pathol. 66: 361-365.
  • Referans13 Ilhan N, Seçkin D. (2005). Protective effect of Nigella sativa seeds on CCl4-induced hepatotoxicity. FÜ Sağ Bil Tıp Derg. 19: 175-179.
  • Referans14 Khorshid HR, Azonov JA, Novitsky YA, Farzamfar B, Shahhosseiny MH. (2008). Hepatoprotective effects of setarud against carbon tetrachloride-induced liver injury in rats. Indian J Gastroenterol. 27: 110-112.
  • Referans15 Balderas Renteria I, Camacho Corona M, Carranzo Rozales P. (2007). Hepatoprotective effect of Leucophyllum frutescens on Wistar albino rats intoxicated with carbon tetrachloride. Ann Hepatol. 6: 251-254.
  • Referans16 Nkosi CZ, Opoku AR, Terblanche SE. (2005). Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4‐induced liver injury in low‐protein fed rats. Phytotherap Res. 19(4): 341-345.
  • Referans17 Al Zuhair HANA, El-Fattah AAA, El-Sayed MI. (2000). Pumpkin-seed oil modulates the effect of felodipine and captopril in spontaneously hypertensive rats. Pharmacol Res. 41(5): 555-563.
  • Referans18 Hashemi JM. (2013). Pumpkin seed oil and vitamin E improve reproductive function of male rats inflicted by testicular injury. World Appl Sci J. 23(10): 1351-1359.
  • Referans19 Eraslan G, Kanbur M, Aslan Ö, Karabacak M. (2013). The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice. Environ Toxicol. 28(12): 681-688.
  • Referans20 Atasever A, Sentürk M, Ekebaş G, Yaman Gram D, Eren M. Ratlarda karbon tetraklorür ile oluşturulan kronik karaciğer hasarı üzerine kabak çekirdeği yağının etkisi ve kaspaz aktivitesi ile hepatik apoptozisin belirlenmesi. ERÜ BAP, TCD-2016-6791 kodlu proje, 2017. https://avesis.erciyes.edu.tr/dosya?id=ee2819ed-6991-48b6-9d03-2b4430764357
  • Referans21 Feroz Khan Z, Asdaq SMB, Prasanna Kumar SR. (2009). Effects of few Indian medicinal herbs on carbon tetrachloride induced hepatic injury in animals. International J Pharm Tech Res. 1: 579-587.
  • Referans22 Venukumar MR, Latha MS. (2002). Hepatoprotective effect of the methanolic extract Curculigo orchioides in CCl4 treated male rats. Indian J Pharmacol. 34: 269- 275.
  • Referans23 Lida C, Fujii K, Koga E, et al. (2009). Effect of alpha-tocopherol on carbon tetrachloride intoxication in the rat liver. Arch Toxicol. 83: 477-483.
  • Referans24 Lv P, Luo HS, Zhou XP, et al. (2006). Thalidomide prevents rat liver cirrhosis via inhibition of oxidative stress. Pathol Res Pract. 202: 777-788.
  • Referans25 Tasci I, Mas N, Mas MR, Tuncer M, Comert B. (2008). Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury. World J Gastroenterol. 14: 4897-4902.
  • Referans26 Radi R. (2018). Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine. Proc Natl Acad Sci. 115: 5839-5848.
  • Referans27 Makni M, Fetoui H, Gargouri NK, et al. (2008). Hypolipidemic and hepatoprotective effects of flax and pumpkin seed mixture rich in ω-3 and ω-6 fatty acids in hypercholesterolemic rats. Food Chem Toxicol. 46(12): 3714-3720.
  • Referans28 Phillips KM, Ruggio DM, Ashraf- Khorassani M. (2005). Phytosterol Composition of Nuts and Seeds Commonly Consumed in the United States. J Agric Food Chem. 53: 9436-9445.
  • Referans29 Ryan E, Galvin K, O’connor TP, Maguire AR, O’brien NM. (2007). Phytosterol, squalene, tocopherol content and fatty acid profile of selected seeds, grains, and legumes. Plant Food Hum Nutr. 62(3): 85-91.
  • Referans30 Lazos ES. (1986). Nutritional, Fatty Acids and Oil Characteristics of Pumpkin and Melon Seeds. J Food Sci. 51(5): 1382-1383.
  • Referans31 Mohamed RA, Ramadan RS, Ahmed LA. (2009). Effect of substituting pumpkin seed protein isolate for casein on serum liver enzymes, lipid profile and antioxidant enzymes in CCl4-intoxicated rats. Adv Biol Res. 3(1-2): 9-15.
  • Referans32 Aal FSA. (2014). The Protective Effect of Pumpkin Seed Oil on Azathioprine–Induced Hepatic Toxicity in Adult Male Albino Rats: Histological and Immunohistochemical Study. Basic Sci Med. 3(4):85-100.
  • Referans33 Seif HSA. (2014). Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni-Suef Univ J Basic Appl Sci. 3(3): 178-185.
  • Referans34 Fawzy EI, El Makawy AI, El-Bamby MM, Elhamalawy HO. (2018). Improved effect of pumpkin seed oil against the bisphenol-A adverse effects in male mice. Toxicol Rep. 5: 857-863.
  • Referans35 Nkosi CZ, Opoku AR, Terblanche SE. (2006). In Vitro antioxidative activity of pumpkin seed (Cucurbita pepo) protein isolate and its In Vivo effect on alanine transaminase and aspartate transaminase in acetaminophen induced liver injury in low protein fed rats. Phytother. 20(9): 780-783.
  • Referans36 Guo XL, Liang B, Wang XW, et al. (2013). Glycyrrhizic acid attenuates CCl4-induced hepatocyte apoptosis in rats via a p53-mediated pathway. World J Gastroenterol. 19(24): 3781- 3791.
  • Referans37 Tao LL, Cheng YY, Ding D, et al. (2012). C/EBP-α ameliorates CCl4-induced liver fibrosis in mice through promoting apoptosis of hepatic stellate cells with little apoptotic effect on hepatocytes in vitro and in vivo. Apoptosis. 17(5): 492-502.
  • Referans38 Parajuli DR, Park EJ, Che XH, et al. (2013). PF2401-SF, standardized fraction of Salvia miltiorrhiza, induces apoptosis of activated hepatic stellate cells in vitro and in vivo. Molecules. 18(2): 2122-2134.
  • Referans39 Aram G, Potter JJ, Liu X, Torbenson MS, Mezey E. (2008). Lack of inducible nitric oxide synthase leads to increased hepatic apoptosis and decreased fibrosis in mice after chronic carbon tetrachloride administration. Hepatol. 47(6): 2051-2058.
  • Referans40 Edfawy M, Hassan MH, Mansour A, Hamed AA, Amin HAA. (2012). Meloxicam Modulates Oxidative Stress Status, Inhibits Prostaglandin E2, and Abrogates Apoptosis in Carbon Tetrachloride–Induced Rat Hepatic Injury. Int J Toxicol. 31(3): 276-286.
  • Referans41 Lee TY, Chang HH, Wang GJ, Chiu JH, Yang YY, Lin HC. (2006). Water‐soluble extract of Salvia miltiorrhiza ameliorates carbon tetrachloride mediated hepatic apoptosis in rats. J Pharm Pharmacol. 58(5): 659-665.
  • Referans42 Liu H, Wang Z, Nowicki MJ. (2014). Caspase-12 mediates carbon tetrachloride-induced hepatocyte apoptosis in mice. World J Gastroenterol. 20(48): 18189-18198.
  • Referans43 Ahmed FE, Selim AO, Selim SA, Mohamed A. (2015). Modulating effect of the aerial parts of pumpkin methanol extract in liver and pancreatic islets on experimental diabetic rat: histologıcal, biochemıcal and immunochemical studies. Cell Tissue Res. 15(2): 4999-5011.
  • Referans44 Evans GO. (2009). Animal Clinical Biochemistry. 2nd Ed. s. 1-344. Taylor & Francis Group, USA.
  • Referans45 Endoh D, Okui T, Ozawa S, et al. (2002). Protective effect of a lignan-containing flaxseed extract against CCl4-induced hepatic injury. J Vet Med Sci. 64(9): 761-765.
  • Referans46 Kasote DM, Badhe YS, Zanwar AA, Hegde MV, Deshmukh KK. (2012). Hepatoprotective potential of ether insoluble phenolic components of n-butanol fraction (EPC-BF) of flaxseed against CCl4-induced liver damage in rats. J Pharm Bioallied Sci. 4(3): 231-235.
  • Referans47 Khanchandani R, Singh SP, Agarwal A. (2014). Role of omega-3 fatty acid in hepatoprotection against carbon tetra chloride induced liver injury in albino rabbits. J Biomed Pharm Res. 3(6): 131-135.
  • Referans48 Niki E, Yoshida Y, Saito Y, Noguchi N. (2005). Lipid peroxidation: Mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun. 338: 668–676.

Year 2020, Volume: 13 Issue: 1, 24 - 32, 30.06.2020

Ayhan Atasever Elife Sayar Görkem Ekebaş Meryem Şentürk Meryem Eren

Abstract

Project Number

TYL-2018-8449

References

  • Referans1 Crawford JM (2003). Karaciğer ve safra yolları. (İçinde): Robbins Temel Patoloji. Robbins SL, Cotran RS, Kumar V (editörler). 7. Baskı, s. 591-630. Nobel Tıp Kitabevi, İstanbul, Türkiye.
  • Referans2 Recknagel RO, Glende EA Jr, Dolak JA, Waller RL. (1989). Mechanisms of carbon tetrachloride toxicity. Pharmacol Therapeut. 43: 139-154.
  • Referans3 Muriel P, Mourelle M. (1990). Prevention by silymarin of membrane alterations in acute CCl4 liver damage. J Appl Toxicol. 10: 275-279.
  • Referans4 Basu S (2003). Carbon tetrachloride-induced lipid peroxidation: Eicosanoid formation and their regulation by antioxidant nutrients. Toxicol. 189:113-127.
  • Referans5 Manibusan MK, Odin M, Eastmond DA. (2007). Postulated carbon tetrachloride mode of action: A review. J Environ Sci Heal C. 25: 185-209.
  • Referans6 Kalaycıoğlu L, Serpek B, Nizamlıoğlu M, Başpınar N, Tiftik AM (2000). Biyokimya. s. 450-496. Nobel Yayın Dağıtım Ltd. Şti, Ankara, Türkiye.
  • Referans7 Nkosi CZ, Opoku AR, Terblanche SE. (2006). Antioxidative effects of pumpkin seed (Cucurbita pepo) protein isolate in CCl4‐Induced liver injury in low‐protein fed rats. Phytotherap. Res. 20(11): 935-940.
  • Referans8 Etim OE, Akpan EJ, Usoh IF. (2008). Hepatotoxicity of carbon tetrachloride: Protective effect of Gongronema latifolium. J Pharm Sci. 21: 268-274.
  • Referans9 Gnanaprakash K, Madhusudhana CC, Ramkanth S, et al. (2010). Aqueous extract of Flacourtia indica prevents carbon tetrachloride induced hepatotoxicity in rat. Int J Biol Sci. 6: 51-55.
  • Referans10 Yehia HM, Al Olayan EM, Elkhadragy MF. (2013). Hepatoprotective role of the pomegranate (Punica granatum) juice on carbon tetrachloride induced oxidative stress in rats. Life Sci J. 10: 1534-1544.
  • Referans11 Zuinen R, Yamaji K, Aoki M, Chikuma T, Hojo H. (2007). Early induced, high-level interleukin-6 expression in the rat peritoneal cavity into which a hepatotoxicant carbon tetrachloride was administered. Toxicol Lett. 170: 42-48.
  • Referans12 Atasever A, Yaman D. (2014). The effects of grape seed and colchicine on carbon tetrachloride induced hepatic damage in rats. Exp Toxicol Pathol. 66: 361-365.
  • Referans13 Ilhan N, Seçkin D. (2005). Protective effect of Nigella sativa seeds on CCl4-induced hepatotoxicity. FÜ Sağ Bil Tıp Derg. 19: 175-179.
  • Referans14 Khorshid HR, Azonov JA, Novitsky YA, Farzamfar B, Shahhosseiny MH. (2008). Hepatoprotective effects of setarud against carbon tetrachloride-induced liver injury in rats. Indian J Gastroenterol. 27: 110-112.
  • Referans15 Balderas Renteria I, Camacho Corona M, Carranzo Rozales P. (2007). Hepatoprotective effect of Leucophyllum frutescens on Wistar albino rats intoxicated with carbon tetrachloride. Ann Hepatol. 6: 251-254.
  • Referans16 Nkosi CZ, Opoku AR, Terblanche SE. (2005). Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4‐induced liver injury in low‐protein fed rats. Phytotherap Res. 19(4): 341-345.
  • Referans17 Al Zuhair HANA, El-Fattah AAA, El-Sayed MI. (2000). Pumpkin-seed oil modulates the effect of felodipine and captopril in spontaneously hypertensive rats. Pharmacol Res. 41(5): 555-563.
  • Referans18 Hashemi JM. (2013). Pumpkin seed oil and vitamin E improve reproductive function of male rats inflicted by testicular injury. World Appl Sci J. 23(10): 1351-1359.
  • Referans19 Eraslan G, Kanbur M, Aslan Ö, Karabacak M. (2013). The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice. Environ Toxicol. 28(12): 681-688.
  • Referans20 Atasever A, Sentürk M, Ekebaş G, Yaman Gram D, Eren M. Ratlarda karbon tetraklorür ile oluşturulan kronik karaciğer hasarı üzerine kabak çekirdeği yağının etkisi ve kaspaz aktivitesi ile hepatik apoptozisin belirlenmesi. ERÜ BAP, TCD-2016-6791 kodlu proje, 2017. https://avesis.erciyes.edu.tr/dosya?id=ee2819ed-6991-48b6-9d03-2b4430764357
  • Referans21 Feroz Khan Z, Asdaq SMB, Prasanna Kumar SR. (2009). Effects of few Indian medicinal herbs on carbon tetrachloride induced hepatic injury in animals. International J Pharm Tech Res. 1: 579-587.
  • Referans22 Venukumar MR, Latha MS. (2002). Hepatoprotective effect of the methanolic extract Curculigo orchioides in CCl4 treated male rats. Indian J Pharmacol. 34: 269- 275.
  • Referans23 Lida C, Fujii K, Koga E, et al. (2009). Effect of alpha-tocopherol on carbon tetrachloride intoxication in the rat liver. Arch Toxicol. 83: 477-483.
  • Referans24 Lv P, Luo HS, Zhou XP, et al. (2006). Thalidomide prevents rat liver cirrhosis via inhibition of oxidative stress. Pathol Res Pract. 202: 777-788.
  • Referans25 Tasci I, Mas N, Mas MR, Tuncer M, Comert B. (2008). Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury. World J Gastroenterol. 14: 4897-4902.
  • Referans26 Radi R. (2018). Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine. Proc Natl Acad Sci. 115: 5839-5848.
  • Referans27 Makni M, Fetoui H, Gargouri NK, et al. (2008). Hypolipidemic and hepatoprotective effects of flax and pumpkin seed mixture rich in ω-3 and ω-6 fatty acids in hypercholesterolemic rats. Food Chem Toxicol. 46(12): 3714-3720.
  • Referans28 Phillips KM, Ruggio DM, Ashraf- Khorassani M. (2005). Phytosterol Composition of Nuts and Seeds Commonly Consumed in the United States. J Agric Food Chem. 53: 9436-9445.
  • Referans29 Ryan E, Galvin K, O’connor TP, Maguire AR, O’brien NM. (2007). Phytosterol, squalene, tocopherol content and fatty acid profile of selected seeds, grains, and legumes. Plant Food Hum Nutr. 62(3): 85-91.
  • Referans30 Lazos ES. (1986). Nutritional, Fatty Acids and Oil Characteristics of Pumpkin and Melon Seeds. J Food Sci. 51(5): 1382-1383.
  • Referans31 Mohamed RA, Ramadan RS, Ahmed LA. (2009). Effect of substituting pumpkin seed protein isolate for casein on serum liver enzymes, lipid profile and antioxidant enzymes in CCl4-intoxicated rats. Adv Biol Res. 3(1-2): 9-15.
  • Referans32 Aal FSA. (2014). The Protective Effect of Pumpkin Seed Oil on Azathioprine–Induced Hepatic Toxicity in Adult Male Albino Rats: Histological and Immunohistochemical Study. Basic Sci Med. 3(4):85-100.
  • Referans33 Seif HSA. (2014). Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni-Suef Univ J Basic Appl Sci. 3(3): 178-185.
  • Referans34 Fawzy EI, El Makawy AI, El-Bamby MM, Elhamalawy HO. (2018). Improved effect of pumpkin seed oil against the bisphenol-A adverse effects in male mice. Toxicol Rep. 5: 857-863.
  • Referans35 Nkosi CZ, Opoku AR, Terblanche SE. (2006). In Vitro antioxidative activity of pumpkin seed (Cucurbita pepo) protein isolate and its In Vivo effect on alanine transaminase and aspartate transaminase in acetaminophen induced liver injury in low protein fed rats. Phytother. 20(9): 780-783.
  • Referans36 Guo XL, Liang B, Wang XW, et al. (2013). Glycyrrhizic acid attenuates CCl4-induced hepatocyte apoptosis in rats via a p53-mediated pathway. World J Gastroenterol. 19(24): 3781- 3791.
  • Referans37 Tao LL, Cheng YY, Ding D, et al. (2012). C/EBP-α ameliorates CCl4-induced liver fibrosis in mice through promoting apoptosis of hepatic stellate cells with little apoptotic effect on hepatocytes in vitro and in vivo. Apoptosis. 17(5): 492-502.
  • Referans38 Parajuli DR, Park EJ, Che XH, et al. (2013). PF2401-SF, standardized fraction of Salvia miltiorrhiza, induces apoptosis of activated hepatic stellate cells in vitro and in vivo. Molecules. 18(2): 2122-2134.
  • Referans39 Aram G, Potter JJ, Liu X, Torbenson MS, Mezey E. (2008). Lack of inducible nitric oxide synthase leads to increased hepatic apoptosis and decreased fibrosis in mice after chronic carbon tetrachloride administration. Hepatol. 47(6): 2051-2058.
  • Referans40 Edfawy M, Hassan MH, Mansour A, Hamed AA, Amin HAA. (2012). Meloxicam Modulates Oxidative Stress Status, Inhibits Prostaglandin E2, and Abrogates Apoptosis in Carbon Tetrachloride–Induced Rat Hepatic Injury. Int J Toxicol. 31(3): 276-286.
  • Referans41 Lee TY, Chang HH, Wang GJ, Chiu JH, Yang YY, Lin HC. (2006). Water‐soluble extract of Salvia miltiorrhiza ameliorates carbon tetrachloride mediated hepatic apoptosis in rats. J Pharm Pharmacol. 58(5): 659-665.
  • Referans42 Liu H, Wang Z, Nowicki MJ. (2014). Caspase-12 mediates carbon tetrachloride-induced hepatocyte apoptosis in mice. World J Gastroenterol. 20(48): 18189-18198.
  • Referans43 Ahmed FE, Selim AO, Selim SA, Mohamed A. (2015). Modulating effect of the aerial parts of pumpkin methanol extract in liver and pancreatic islets on experimental diabetic rat: histologıcal, biochemıcal and immunochemical studies. Cell Tissue Res. 15(2): 4999-5011.
  • Referans44 Evans GO. (2009). Animal Clinical Biochemistry. 2nd Ed. s. 1-344. Taylor & Francis Group, USA.
  • Referans45 Endoh D, Okui T, Ozawa S, et al. (2002). Protective effect of a lignan-containing flaxseed extract against CCl4-induced hepatic injury. J Vet Med Sci. 64(9): 761-765.
  • Referans46 Kasote DM, Badhe YS, Zanwar AA, Hegde MV, Deshmukh KK. (2012). Hepatoprotective potential of ether insoluble phenolic components of n-butanol fraction (EPC-BF) of flaxseed against CCl4-induced liver damage in rats. J Pharm Bioallied Sci. 4(3): 231-235.
  • Referans47 Khanchandani R, Singh SP, Agarwal A. (2014). Role of omega-3 fatty acid in hepatoprotection against carbon tetra chloride induced liver injury in albino rabbits. J Biomed Pharm Res. 3(6): 131-135.
  • Referans48 Niki E, Yoshida Y, Saito Y, Noguchi N. (2005). Lipid peroxidation: Mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun. 338: 668–676.
There are 48 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research
Authors

Ayhan Atasever 0000-0002-6327-1604

Elife Sayar 0000-0002-9776-1572

Görkem Ekebaş 0000-0001-9094-677X

Meryem Şentürk 0000-0002-3763-8310

Meryem Eren 0000-0003-1339-0493

Project Number TYL-2018-8449
Publication Date June 30, 2020
Acceptance Date May 7, 2020
Published in Issue Year 2020 Volume: 13 Issue: 1

Cite

APA Atasever, A., Sayar, E., Ekebaş, G., Şentürk, M., et al. (2020). Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 24-32.
AMA Atasever A, Sayar E, Ekebaş G, Şentürk M, Eren M. Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi. Dicle Üniv Vet Fak Derg. June 2020;13(1):24-32.
Chicago Atasever, Ayhan, Elife Sayar, Görkem Ekebaş, Meryem Şentürk, and Meryem Eren. “Ratlarda Karbon Tetraklorür Ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi Ve Kaspaz Aktivitesi Ile Hepatik Apoptozisin Belirlenmesi”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13, no. 1 (June 2020): 24-32.
EndNote Atasever A, Sayar E, Ekebaş G, Şentürk M, Eren M (June 1, 2020) Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13 1 24–32.
IEEE A. Atasever, E. Sayar, G. Ekebaş, M. Şentürk, and M. Eren, “Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi”, Dicle Üniv Vet Fak Derg, vol. 13, no. 1, pp. 24–32, 2020.
ISNAD Atasever, Ayhan et al. “Ratlarda Karbon Tetraklorür Ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi Ve Kaspaz Aktivitesi Ile Hepatik Apoptozisin Belirlenmesi”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13/1 (June 2020), 24-32.
JAMA Atasever A, Sayar E, Ekebaş G, Şentürk M, Eren M. Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi. Dicle Üniv Vet Fak Derg. 2020;13:24–32.
MLA Atasever, Ayhan et al. “Ratlarda Karbon Tetraklorür Ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi Ve Kaspaz Aktivitesi Ile Hepatik Apoptozisin Belirlenmesi”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 13, no. 1, 2020, pp. 24-32.
Vancouver Atasever A, Sayar E, Ekebaş G, Şentürk M, Eren M. Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi. Dicle Üniv Vet Fak Derg. 2020;13(1):24-32.
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DANIŞMA KURULU
Prof. Dr. Abit AKTAŞ, İstanbul Üniversitesi-Cerrahpaşa
Prof. Dr. Yılmaz ARAL, Ankara Üniversitesi
Prof. Dr. Ali ARSLAN, Fırat Üniversitesi, Elazığ
Prof. Dr. M. Sedat BARAN, Dicle Üniversitesi, Diyarbakır
Prof. Dr. Cengiz CEYLAN, Balıkesir Üniversitesi
Prof. Dr. Ahmet ÇAKIR, Ankara Üniversitesi
Prof. Dr. Burhan ÇETİNKAYA, Fırat Üniversitesi, Elazığ
Prof. Dr. Ülker EREN, Adnan Menderes Üniversitesi, Aydın
Prof. Dr. Abdurrahman GÜL, Bingöl Üniversitesi
Prof. Dr. Hakki KARA, Cumhuriyet Üniversitesi, Sivas
Prof. Dr. Narin LİMAN, Erciyes Üniversitesi, Kayseri
Prof. Dr. Abdullah ÖZEN, Fırat Üniversitesi, Elazığ
Prof. Dr. Ayşe SERBEST, Uludağ Üniversitesi, Bursa
Prof. Dr. Kazım ŞAHİN, Fırat Üniversitesi, Elazığ
Prof. Dr. Tekin ŞAHİN, Siirt Üniversitesi
Prof. Dr. Nihat ŞINDAK, Siirt Üniversitesi
Prof. Dr. Muzaffer TAŞ, Namık Kemal Üniversitesi, Tekirdağ
Prof. Dr. Necati TİMURKAN Fırat Üniversitesi, Elazığ
Prof. Dr. Emine ÜNSALDI, Fırat Üniversitesi, Elazığ
Prof. Dr. Murat YILDIRIM, Kırıkkale Üniversitesi
Doç. Dr. M. Osman ATLI, Harran Üniversitesi, Şanlıurfa
Doç. Dr. Bülent ELİTOK, Afyon Kocatepe Üniversitesi
Doç. Dr. Serkan ERDOĞAN, Namık Kemal Üniversitesi, Tekirdağ
Dr. Öğr. Üyesi Zelal KARAKOÇ, Dicle Üniversitesi, Diyarbakır
Dr. Öğr. Üyesi Ersin UYSAL, Dicle Üniversitesi, Diyarbakır
*Not: Yayın ve Danışma Kurulu Üyeleri alfabetik sıraya göre dizilmiştir.