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Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses

Year 2020, Volume: 13 Issue: 1, 20 - 23, 30.06.2020

Fatma Çolakoğlu Muhammet Lütfi Selçuk Hasan Hüseyin Dönmez

Abstract

The purpose of this research evaluated the effects of methimazole used to create hypothyroidism in rats, and its effects on the eosinophilic cells and uterus layer thicknesses. Uterus samples, 15 healthy Wistar Albino female rats and 12-14 weeks of age, were taken from control (C, n=6) and experimental (E, n=9) groups. Intraperitoneally, methimazole was injected as 10 mg/kg/day dose in the Group E for 2 weeks. After hypothyroidism was created in the Group E, feeding all the rats in this study was continued with normal pellet feed again for 2 weeks to see chronic effects on tissue. At the end of the 4th week, uterus samples were stained with Crossmon’s trichrome staining. Uterus layer thicknesses were obtained by measuring from images of six different uterus regions including endometrium, myometrium and perimetrium layers of each preparation with ImageJ Analysis Program. The mean eosinophil granulocyte count of the endometrium was higher in the Group E when compared to Group C (P<0.05). This cell distribution was concentrated in the endometrium functionalis. However, there was no difference in uterine layer thicknesses between the groups (P>0.05). We concluded that methimazole caused hypereosinophil granulocyte accumulation in the endometrium functionalis. This study is important for clinicians to evaluate methimazole-associated eosinophilic infiltration due to hypersensitive immune reactions to the drug, especially corcerning pregnancy.

Keywords

Endometrium hypereosinophilic infiltration methimazole rat

References

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  • 2. Klecha AJ, Genaro AM, Lysionek AE, Caro RA, Coluccia AG, Cremaschi GA. (2000). Experimental evidence pointing to the bidirectional interaction between the immune system and the thyroid axis. Int J Immunopharmacol. 22: 491-500.
  • 3. Homsanit M, Sriussadapom S, Vannasaeng S, Peerapatdit T, Nitiyanant W, Vichayanrat A. (2001). Efficacy of single daily dosage of methimazole vs. propylthiouracil in the induction of euthyroidism. Clin Endocrinol. 54: 385-390.
  • 4. Farwell AP, Braverman LE. (2001). Thyroid and Antithyroid Drugs. In: Goodman and Gilman’s The Pharmacological Basis of Therapeutics. Hardman JG, Limbird LE (eds). pp. 1383-1410. McGraw-Hill, USA.
  • 5. Akın S, Inanır A. (2013). Evaluation of musculoskeletal system symptoms in patients with goiter. Cukurova Med J. 38: 261-269.
  • 6. Bou Khalil R, Abou Salbi M, Sissi S, et al. (2013). Methimazole-induced myositis: a case report and review of the literature. Endocrinol. 13: 1-4.
  • 7. Maruyama T, Masuda H, Ono M, Kajitani T, Yoshimura Y. (2010). Human uterus stem/progenitor cells: their possible role in uterus physiology and pathology. Reproduction. 140: 11-22.
  • 8. Spencer T, Hayashi K, Hu J, Carpenter KD. (2005). Comparative development biology of the mammalian uterus. Curr Top Dev Biol. 68: 85-122.
  • 9. Gartner LP, Hiatt JL. (2007): Blood and Hemopoiesis. In: Color Textbook of Histology. Gartner LP, Hiatt JL (eds). 3rd ed. pp. 219-249. Elsevier, Philadelphia, USA.
  • 10. Shamri R, Xenakis JJ, Spencer LA. (2011). Eosinophils in innate immunity: an evolving story. Cell Tissue Res. 343: 57-83.
  • 11. Jacobsen EA, Helmers RA, Lee JJ, Lee NA. (2012). The expanding role(s) of eosinophils in health and disease. Blood. 120: 3882-3890.
  • 12. Swann AC. (1989). Noradrenaline and thyroid function regulate (Na+-K+) adenosine triphosphate independently in vivo. Eur J Pharmacol. 169: 275-283.
  • 13. Selçuk ML, Tıpırdamaz S. (2020). A morphological and stereological study on brain, cerebral hemispheres and cerebellum of New Zealand rabbits. Anat Histol Embryol. 49: 90-96.
  • 14. Schneider CA, Rasband WS, Eliceiri KW. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods. 9: 671-675.
  • 15. Cooper DS. (2005). Antityhroid drugs. N Eng J Med. 352: 905-917.
  • 16. De Vito P, Incerpi S, Pederson JZ, Luly P, Davis FB, Davis PJ. (2011). Thyroid hormones as modulators of immune activities at the cellular level. Thyroid. 21: 879-890.
  • 17. Gaspar-da-Costa P, Silva FD, Henriques J, et al. (2017). Methimazole associated eosinophilic pleural effusion: a case report. BMC Pharmacol and Toxicol. 18,: 1-4.
  • 18. Blumenthal RD, Samoszuk M, Taylor AP, Brown G, Alisauskas R, Goldenberg DM. (2000). Degranulating eosinophils in human endometriosis. Am J Pathol. 156: 1581-1588.
  • 19. Dutta M, Talukdar KL. (2015). A histological study of uterus in reproductive and postmenopausal women. NJCA. 4: 17-25.
  • 20. Al-Qudsi F, Linjawi S. (2012). Histological and hormonal changes in rat endometrium under the effect of camphor. Life Science Journal. 9(2): 348-355.
  • 21. Ye Q, Zhang Y, Fu J, et al. (2019). Effect of Ligustrazine on Endometrium Injury of Thin Endometrium Rats. Evid Based Complement Alternat Med. Vol 2019, Article ID 7161906, 7 pages.
  • 22. Zhao J, Zhang Q, Wang Y, Li Y. (2015). Uterine infusion with bone marrow mesenchymal stem cells improves endometrium thickness in a rat model of thin endometrium. Reprod Sci. 22(2): 181–188.
  • 23. Ekizceli G, İnan S, Öktem G, Onur E, Özbilgin K. (2015). Sıçanlarda östrus döngüsü ile ilişkili ovaryum ve uterusların histolojik değerlendirmesi. Uludağ Üniv Tıp Fak Derg. 41(2): 65-72.

Year 2020, Volume: 13 Issue: 1, 20 - 23, 30.06.2020

Fatma Çolakoğlu Muhammet Lütfi Selçuk Hasan Hüseyin Dönmez

Abstract

References

  • 1. Lu CH, Hsieh CH. (2007). Elevation of creatine kinase during medical treatment of Grave’s disease. Med Sci. 27: 241-244.
  • 2. Klecha AJ, Genaro AM, Lysionek AE, Caro RA, Coluccia AG, Cremaschi GA. (2000). Experimental evidence pointing to the bidirectional interaction between the immune system and the thyroid axis. Int J Immunopharmacol. 22: 491-500.
  • 3. Homsanit M, Sriussadapom S, Vannasaeng S, Peerapatdit T, Nitiyanant W, Vichayanrat A. (2001). Efficacy of single daily dosage of methimazole vs. propylthiouracil in the induction of euthyroidism. Clin Endocrinol. 54: 385-390.
  • 4. Farwell AP, Braverman LE. (2001). Thyroid and Antithyroid Drugs. In: Goodman and Gilman’s The Pharmacological Basis of Therapeutics. Hardman JG, Limbird LE (eds). pp. 1383-1410. McGraw-Hill, USA.
  • 5. Akın S, Inanır A. (2013). Evaluation of musculoskeletal system symptoms in patients with goiter. Cukurova Med J. 38: 261-269.
  • 6. Bou Khalil R, Abou Salbi M, Sissi S, et al. (2013). Methimazole-induced myositis: a case report and review of the literature. Endocrinol. 13: 1-4.
  • 7. Maruyama T, Masuda H, Ono M, Kajitani T, Yoshimura Y. (2010). Human uterus stem/progenitor cells: their possible role in uterus physiology and pathology. Reproduction. 140: 11-22.
  • 8. Spencer T, Hayashi K, Hu J, Carpenter KD. (2005). Comparative development biology of the mammalian uterus. Curr Top Dev Biol. 68: 85-122.
  • 9. Gartner LP, Hiatt JL. (2007): Blood and Hemopoiesis. In: Color Textbook of Histology. Gartner LP, Hiatt JL (eds). 3rd ed. pp. 219-249. Elsevier, Philadelphia, USA.
  • 10. Shamri R, Xenakis JJ, Spencer LA. (2011). Eosinophils in innate immunity: an evolving story. Cell Tissue Res. 343: 57-83.
  • 11. Jacobsen EA, Helmers RA, Lee JJ, Lee NA. (2012). The expanding role(s) of eosinophils in health and disease. Blood. 120: 3882-3890.
  • 12. Swann AC. (1989). Noradrenaline and thyroid function regulate (Na+-K+) adenosine triphosphate independently in vivo. Eur J Pharmacol. 169: 275-283.
  • 13. Selçuk ML, Tıpırdamaz S. (2020). A morphological and stereological study on brain, cerebral hemispheres and cerebellum of New Zealand rabbits. Anat Histol Embryol. 49: 90-96.
  • 14. Schneider CA, Rasband WS, Eliceiri KW. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods. 9: 671-675.
  • 15. Cooper DS. (2005). Antityhroid drugs. N Eng J Med. 352: 905-917.
  • 16. De Vito P, Incerpi S, Pederson JZ, Luly P, Davis FB, Davis PJ. (2011). Thyroid hormones as modulators of immune activities at the cellular level. Thyroid. 21: 879-890.
  • 17. Gaspar-da-Costa P, Silva FD, Henriques J, et al. (2017). Methimazole associated eosinophilic pleural effusion: a case report. BMC Pharmacol and Toxicol. 18,: 1-4.
  • 18. Blumenthal RD, Samoszuk M, Taylor AP, Brown G, Alisauskas R, Goldenberg DM. (2000). Degranulating eosinophils in human endometriosis. Am J Pathol. 156: 1581-1588.
  • 19. Dutta M, Talukdar KL. (2015). A histological study of uterus in reproductive and postmenopausal women. NJCA. 4: 17-25.
  • 20. Al-Qudsi F, Linjawi S. (2012). Histological and hormonal changes in rat endometrium under the effect of camphor. Life Science Journal. 9(2): 348-355.
  • 21. Ye Q, Zhang Y, Fu J, et al. (2019). Effect of Ligustrazine on Endometrium Injury of Thin Endometrium Rats. Evid Based Complement Alternat Med. Vol 2019, Article ID 7161906, 7 pages.
  • 22. Zhao J, Zhang Q, Wang Y, Li Y. (2015). Uterine infusion with bone marrow mesenchymal stem cells improves endometrium thickness in a rat model of thin endometrium. Reprod Sci. 22(2): 181–188.
  • 23. Ekizceli G, İnan S, Öktem G, Onur E, Özbilgin K. (2015). Sıçanlarda östrus döngüsü ile ilişkili ovaryum ve uterusların histolojik değerlendirmesi. Uludağ Üniv Tıp Fak Derg. 41(2): 65-72.
There are 23 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Fatma Çolakoğlu 0000-0003-0410-5523

Muhammet Lütfi Selçuk 0000-0002-9915-3829

Hasan Hüseyin Dönmez 0000-0003-4664-8489

Publication Date June 30, 2020
Acceptance Date May 2, 2020
Published in Issue Year 2020 Volume: 13 Issue: 1

Cite

APA Çolakoğlu, F., Selçuk, M. L., & Dönmez, H. H. (2020). Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 20-23.
AMA Çolakoğlu F, Selçuk ML, Dönmez HH. Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniv Vet Fak Derg. June 2020;13(1):20-23.
Chicago Çolakoğlu, Fatma, Muhammet Lütfi Selçuk, and Hasan Hüseyin Dönmez. “Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13, no. 1 (June 2020): 20-23.
EndNote Çolakoğlu F, Selçuk ML, Dönmez HH (June 1, 2020) Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13 1 20–23.
IEEE F. Çolakoğlu, M. L. Selçuk, and H. H. Dönmez, “Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses”, Dicle Üniv Vet Fak Derg, vol. 13, no. 1, pp. 20–23, 2020.
ISNAD Çolakoğlu, Fatma et al. “Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13/1 (June 2020), 20-23.
JAMA Çolakoğlu F, Selçuk ML, Dönmez HH. Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniv Vet Fak Derg. 2020;13:20–23.
MLA Çolakoğlu, Fatma et al. “Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 13, no. 1, 2020, pp. 20-23.
Vancouver Çolakoğlu F, Selçuk ML, Dönmez HH. Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniv Vet Fak Derg. 2020;13(1):20-3.
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