Research Article
Year 2021,
Volume: 14 Issue: 1, 43 - 47, 30.06.2021
Abstract
References
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- 2. Gül, Y. (2006). Geviş Getiren Hayvanların İç Hastalıkları (Sığır, Koyun-Keçi). II. Baskı. Medipres Matbaacılık Ltd. Şti., Malatya, 452-454.
- 3. Hasegawa M, Iida Y, Yano TA, Takaiwa F, Iwabuchi M, 1985: Phylogenetic relationships among eukaryotic kingdoms inferred from ribosomal RNA sequences. Journal of Molecular Evolution, 22(1), 32-38.
- 4. Papini R, Nardoni S, Fanelli A, Mancianti F, 2009: High infection rate of Trichophyton verrucosum in calves from Central Italy. Zoonoses and public health, 56(2), 59-64.
- 5. Susever S, YEĞENOĞLU Y, 2011: İnvazif Mantar Enfeksiyonlarının Tanımlanmasında Moleküler Yöntemlerin Öneminin Konvansiyonel Yöntemler ile Karşılaştırılarak Değerlendirilmesi. Mikrobiyol Bul, 45(2), 325-335.
- 6. Valente P, Ramos JP, Leoncini O, 1999: Sequencing as a tool in yeast molecular taxonomy. Canadian journal of microbiology, 45(11), 949-958.
- 7. Kallow W, Erhard M, Shah HN, Raptakis E, Welker M, 2010: MALDI-TOF MS for microbial identification: years of experimental development to an established protocol. Mass spectrometry for microbial proteomics, 255, 276.
- 8. Larone, D. H., & Larone, D. H. (1987). Medically important fungi: a guide to identification (Vol. 189). New York: Elsevier.
- 9. Bartosch T, Heydel T, Uhrlaß S,et al. 2018: MALDI-TOF MS analysis of bovine and zoonotic Trichophyton verrucosum isolates reveals a distinct peak and cluster formation of a subgroup with Trichophyton benhamiae. Medical mycology, 56(5), 602-609.
- 10. Faraj MK, Mohammed SJ, 2011: A survey of dermatophytes isolated from cows and sheep in Iraq. The Iraqi journal of veterinary medicine, 35(2), 40-45.
- 11. Aghamirian MR, Ghiasian SA, 2011: Dermatophytes as a cause of epizoonoses in dairy cattle and humans in Iran: epidemiological and clinical aspects. Mycoses, 54(4), 52-56.
- 12. Ozkanlar Y, Aktas MS, Kirecci E, 2009: Mycozoonosis associated with ringworm of calves in Erzurum Province, Turkey. Kafkas Univ Vet Fak Derg. 15, 141-144.
- 13. Tel OY, Bozkaya F, Yiğin A, Gürbilek SE, Keskin O, 2018: Isolation and Molecular Characterization of Bovine Trichophyton verrucosum Strains Based on Sequence Analysis of Internal Transcribed Spacer Region (ITS1) and Microsatellite Loci. Pakistan Veterinary Journal, 38(2).
- 14. Mbata TI, 2009: Dermatophytes and other skin mycoses found in featherless broiler toe webs. Internet J Dermatol, 7(2), 399-402.
- 15. İLHAN Z, 2015: Isolation of dermatophytes from cattle, sheep, goats and Van cats in Van and its around. Van Veterinary Journal, 26(1), 1-5.
- 16. Yahyaraeyat R, Shokri H, Khosravi AR, Soltani M, Erfanmanesh A, Nikaein D, 2009: Occurrence of animals dermatophytosis in Tehran, Iran. World J Zool, 4(3), 200-204
- 17. Khosrav AR, Mahmoudi M, 2003: Dermatophytes isolated from domestic animals in Iran. Mycoses, 46(5‐6), 222-225.
- 18. Yildirim M, Cinar M, Ocal N, Yagc, BB, Askar S, 2010: Prevalence of clinical dermatophytosis and oxidative stress in cattle. Journal of Animal and Veterinary Advances, 9(14), 1978-1982.
- 19. Moretti A, Boncio L, Pasquali P, Fioretti DP, 1998: Epidemiological aspects of dermatophyte infections in horses and cattle. Journal of Veterinary Medicine, Series B, 45(1‐10), 205-208.
- 20. Ming PX, Ti YLX, Bulmer GS, 2006: Outbreak of Trichophyton verrucosum in China transmitted from cows to humans. Mycopathologia, 161(4), 225-228.
- 21. Carlsson J, 1993: Risken för ringorm hos nötkreatur och människa. Svensk Veterınartıdnıng, 45: 467
- 22. Haab C, 1991: Epidemiologie der Trichophytie beim Mastkalb. Inaugural: Doctoral Dissertation. Switzerland, Zurich: University of Zurich.
- 23. Oldenkam EP, 1979: Natamycin treatment of ringworm in cattle in the United Kingdom. The Veterinary Record, 105(24), 5554-5556.
- 24. Eliopoulos GM, Perea S, Patterson TF, 2002: Antifungal resistance in pathogenic fungi. Clinical Infectious Diseases, 35(9), 1073-1080.
- 25. Seng P, Rolain JM, Fournier PE, La Scola B, Drancourt M, Raoult D 2010: MALDI-TOF-mass spectrometry applications in clinical microbiology. Future microbiology, 5(11), 1733-1754.
- 26. Özcan N, Ezi Ö, Akpolat N, Bozdağ H, Mete M, Gül K, 2016: Klinik örneklerde saptanan Candida türlerinin MALDI-TOF MS ile tiplendirilmesi. Dicle Medikal Journal, 43(3): 390-394.
- 27. García MI, Rodríguez S, Muñoz-Bellido JL 2013: Identification of fungal clinical isolates by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Rev Esp Quimioter 2013;26:193-197.
Year 2021,
Volume: 14 Issue: 1, 43 - 47, 30.06.2021
Abstract
Trichophyton verrucosum genç sığırlarda gelişme geriliği, süt sığırlarında verim kaybı ve derinin ekonomik değerinin azalması gibi önemli sorunlara neden olmaktadır. Etken teşhisinde konvansiyonel yöntemlerin zaman alıcı olması ve kesin sonuçlar vermemesinden dolayı duyarlılığı ve özgüllüğü yüksek, hızlı yeni tanı yöntemlerine ihtiyaç duyulmaktadır. Bu çalışmada sığırlardan T. verrucosum’un kültür ve MALDI-TOF MS yöntemleri ile identifikasyonu amaçlanmıştır. Bu amaca yönelik olarak Şanlıurfa ilinde dermatofitoz şüpheli 100 adet sığırdan deri kazıntısı ve kıl örnekleri alındı. Alınan örnekler direkt mikroskopi ile incelendi ve 52 (%52)’si pozitif bulundu. Örneklerin yapılan kültürü sonucu 63 (%63)’ünde dermatofit izole edildi ve bunların 52 (%52)’si T. verrucosum olarak identifiye edildi. Örnek alınan hayvancılık işletmelerinin %100 nün T. verrucosum ile infekte olduğu saptandı. İdentifikasyonu yapılan T. verrucosum izolatları 7950-7954 arasında MALDI-TOF MS spektrum pikleri saptanarak doğrulandı. T. verrucosum’un doğrulanması amacı ile MALDI TOF tekniğinin geleneksel yöntemlere kıyasla daha başarılı bir şekilde kullanılabileceği, etkenin zoonotik önemi ve ülke hayvancılığına verdiği ekonomik zarardan dolayı hastalığa karşı gerekli koruma kontrol önlemlerinin alınması gerektiği sonucuna varıldı.
References
- 1. Neji S, Trabelsi H, Hadrich I, et al. 2016: Molecular characterization of strains of the Trichophyton verrucosum complex from Tunisia. Sabouraudia, 54(8), 787-793.
- 2. Gül, Y. (2006). Geviş Getiren Hayvanların İç Hastalıkları (Sığır, Koyun-Keçi). II. Baskı. Medipres Matbaacılık Ltd. Şti., Malatya, 452-454.
- 3. Hasegawa M, Iida Y, Yano TA, Takaiwa F, Iwabuchi M, 1985: Phylogenetic relationships among eukaryotic kingdoms inferred from ribosomal RNA sequences. Journal of Molecular Evolution, 22(1), 32-38.
- 4. Papini R, Nardoni S, Fanelli A, Mancianti F, 2009: High infection rate of Trichophyton verrucosum in calves from Central Italy. Zoonoses and public health, 56(2), 59-64.
- 5. Susever S, YEĞENOĞLU Y, 2011: İnvazif Mantar Enfeksiyonlarının Tanımlanmasında Moleküler Yöntemlerin Öneminin Konvansiyonel Yöntemler ile Karşılaştırılarak Değerlendirilmesi. Mikrobiyol Bul, 45(2), 325-335.
- 6. Valente P, Ramos JP, Leoncini O, 1999: Sequencing as a tool in yeast molecular taxonomy. Canadian journal of microbiology, 45(11), 949-958.
- 7. Kallow W, Erhard M, Shah HN, Raptakis E, Welker M, 2010: MALDI-TOF MS for microbial identification: years of experimental development to an established protocol. Mass spectrometry for microbial proteomics, 255, 276.
- 8. Larone, D. H., & Larone, D. H. (1987). Medically important fungi: a guide to identification (Vol. 189). New York: Elsevier.
- 9. Bartosch T, Heydel T, Uhrlaß S,et al. 2018: MALDI-TOF MS analysis of bovine and zoonotic Trichophyton verrucosum isolates reveals a distinct peak and cluster formation of a subgroup with Trichophyton benhamiae. Medical mycology, 56(5), 602-609.
- 10. Faraj MK, Mohammed SJ, 2011: A survey of dermatophytes isolated from cows and sheep in Iraq. The Iraqi journal of veterinary medicine, 35(2), 40-45.
- 11. Aghamirian MR, Ghiasian SA, 2011: Dermatophytes as a cause of epizoonoses in dairy cattle and humans in Iran: epidemiological and clinical aspects. Mycoses, 54(4), 52-56.
- 12. Ozkanlar Y, Aktas MS, Kirecci E, 2009: Mycozoonosis associated with ringworm of calves in Erzurum Province, Turkey. Kafkas Univ Vet Fak Derg. 15, 141-144.
- 13. Tel OY, Bozkaya F, Yiğin A, Gürbilek SE, Keskin O, 2018: Isolation and Molecular Characterization of Bovine Trichophyton verrucosum Strains Based on Sequence Analysis of Internal Transcribed Spacer Region (ITS1) and Microsatellite Loci. Pakistan Veterinary Journal, 38(2).
- 14. Mbata TI, 2009: Dermatophytes and other skin mycoses found in featherless broiler toe webs. Internet J Dermatol, 7(2), 399-402.
- 15. İLHAN Z, 2015: Isolation of dermatophytes from cattle, sheep, goats and Van cats in Van and its around. Van Veterinary Journal, 26(1), 1-5.
- 16. Yahyaraeyat R, Shokri H, Khosravi AR, Soltani M, Erfanmanesh A, Nikaein D, 2009: Occurrence of animals dermatophytosis in Tehran, Iran. World J Zool, 4(3), 200-204
- 17. Khosrav AR, Mahmoudi M, 2003: Dermatophytes isolated from domestic animals in Iran. Mycoses, 46(5‐6), 222-225.
- 18. Yildirim M, Cinar M, Ocal N, Yagc, BB, Askar S, 2010: Prevalence of clinical dermatophytosis and oxidative stress in cattle. Journal of Animal and Veterinary Advances, 9(14), 1978-1982.
- 19. Moretti A, Boncio L, Pasquali P, Fioretti DP, 1998: Epidemiological aspects of dermatophyte infections in horses and cattle. Journal of Veterinary Medicine, Series B, 45(1‐10), 205-208.
- 20. Ming PX, Ti YLX, Bulmer GS, 2006: Outbreak of Trichophyton verrucosum in China transmitted from cows to humans. Mycopathologia, 161(4), 225-228.
- 21. Carlsson J, 1993: Risken för ringorm hos nötkreatur och människa. Svensk Veterınartıdnıng, 45: 467
- 22. Haab C, 1991: Epidemiologie der Trichophytie beim Mastkalb. Inaugural: Doctoral Dissertation. Switzerland, Zurich: University of Zurich.
- 23. Oldenkam EP, 1979: Natamycin treatment of ringworm in cattle in the United Kingdom. The Veterinary Record, 105(24), 5554-5556.
- 24. Eliopoulos GM, Perea S, Patterson TF, 2002: Antifungal resistance in pathogenic fungi. Clinical Infectious Diseases, 35(9), 1073-1080.
- 25. Seng P, Rolain JM, Fournier PE, La Scola B, Drancourt M, Raoult D 2010: MALDI-TOF-mass spectrometry applications in clinical microbiology. Future microbiology, 5(11), 1733-1754.
- 26. Özcan N, Ezi Ö, Akpolat N, Bozdağ H, Mete M, Gül K, 2016: Klinik örneklerde saptanan Candida türlerinin MALDI-TOF MS ile tiplendirilmesi. Dicle Medikal Journal, 43(3): 390-394.
- 27. García MI, Rodríguez S, Muñoz-Bellido JL 2013: Identification of fungal clinical isolates by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Rev Esp Quimioter 2013;26:193-197.
There are 27 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research |
| Authors | |
| Publication Date | June 30, 2021 |
| Acceptance Date | April 7, 2021 |
| Published in Issue | Year 2021 Volume: 14 Issue: 1 |
