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Ratlarda Osteoartrit Tedavisinde Mikrokırık Tekniği ve Eklem Içi Sodyum Hiyalüronat Enjeksiyonunun Etkinliğinin Değerlendirilmesi

Year 2021, Volume: 14 Issue: 1, 56 - 61, 30.06.2021
https://doi.org/10.47027/duvetfd.886960

Abstract

Osteoartritisli hayvanlarda eklem ağrısı, hareket etmede isteksizlik, performans kaybı ve topallık gibi klinik belirtiler görülür. Osteoartriti tedavi etmek için birçok medikal ve girişimsel yöntem kullanılmaktadır, ancak tanımlanmış hiçbir tedavi ile henüz hasarlı kıkırdak tamamen onarılamamaktadır. Bu çalışmada, 40 adet erkek Wistar Albino rat; mikrokırık grubu, eklem içi hyaluronik asit grubu, mikro kırık + eklem içi hyaluronik asit grubu ve kontrol grubu olarak (n= 10) ayrılmıştır. Klinik bulgular olarak diz çevresi ile vücut ağırlığı ölçülmüş ve bacak duruşu skorlanmıştır. Radyolojik muayene bulguları eklem aralığı daralması, subkondral kemik sklerozu, osteofit oluşumu olmak üzere 3 veriye dayalı derecelendirme ile değerlendirilmiştir. Ayrıca, deney bitiminde sakrifikasyon sonrasında ratların ilgili eklemi osteoartritin şiddetine göre makroskopik olarak skorlanmıştır. Bacak duruşundaki değişiklikler, diz çevresi ölçümü ve radyolojik bulgular dikkate alındığında, bu çalışmada monosodyum iyodoasetat uygulaması ile osteoartrit gelişimi başarıyla sağlanmıştır. Sunulan çalışmadan elde edilen bulgular doğrultusunda, mikrokırık tekniğinin hyaluronik asit uygulaması ile birleştirilmesinin tedavi sürecine ek katkı sağlamadığı ve mikrokırık veya hyaluronik asit uygulamasının tek başına kullanılmasının daha olumlu sonuçlar verebileceği sonucuna varılmıştır.

Supporting Institution

Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri

Project Number

VTF-16019

References

  • 1. Kikuchi T, Yamada H, Shimmei M (1996). Effect of high molecular weight hyaluronan on cartilage degeneration in a rabbit model of osteoarthritis. Osteoarthr Cartil.4(2):99-110.
  • 2. He Y, Li Z, Alexander PG, et al. (2020). Pathogenesis of osteoarthritis: risk factors, regulatory pathways in chondrocytes, and experimental models. Biology 9(8): 194.
  • 3. Moskowitz RW, Altman RD, Hochberg MC, Buckwalter JA, Goldberg VM (2007). Osteoarthritis: Diagnosis and medical/surgical management. 4th ed., Lippincott, Williams & Wilkins, USA.
  • 4. Kerwin SC (2010). Osteoarthritis in cats. Top Companion Anim Med. 25(4):218-23.
  • 5. Henrotin Y, Sanchez C, Balligand M. (2005) Pharmaceutical and nutraceutical management of canine osteoarthritis: present and future perspectives. Vet J. 170(1):113-23.
  • 6. Goodrich LR, Nixon AJ (2006). Medical treatment of osteoarthritis in the horse – a review. Vet J 171(1):51-69.
  • 7. Rychel JK (2010). Diagnosis and Treatment of Osteoarthritis, Top Companion Anim Med. 25(1):20-25.
  • 8. Steadman J, Rodkey W, Briggs K, Rodrigo J (1999). The microfracture technic in the management of complete cartilage defects in the knee joint. Orthopade. 28:26–32.
  • 9. Sluka KA, Milton MA, Willis WD, Westlund KN (1997). Differential roles of neurokinin 1 and neurokinin 2 receptors in the development and maintenance of heat hyperalgesia induced by acute inflammation Br J Pharmacol. 120(7):1263–1273.
  • 10. Evans RG, Collins C, Miller P, Ponsford FM, Elson CJ (1994). Radiological scoring of osteoarthritis progression in STR/ORT mice. Osteoarthr Cartil.2:103–9.
  • 11. Bennett GA, Waine H, Bauer W (1942). Changes in the knee joint at various ages with particular reference to the nature and development of degenerative joint disease. New York: The Commonwealth Fund.1-93.
  • 12. Clarke KA, Heitmeyer SA, Smith AG, Taiwo YO (1997). Gait analysis in a rat model of osteoarthrosis. Physiol Behav. 62(5):951-954.
  • 13. Xu J, Yan L, Yan B, et al. (2020). Osteoarthritis Pain Model Induced by Intra-Articular Injection of Mono-Iodoacetate in Rats. JoVE (Journal of Visualized Experiments), (159), e60649.
  • 14. Rebai MA, Sahnoun N, Abdelhedi et al. (2020) Animal models of osteoarthritis: characterization of a model induced by Mono-Iodo-Acetate injected in rabbits. Libyan Journal of Medicine, 15(1): 1753943.
  • 15. Fernihough J, Gentry C, Malcangio M, et al. (2004). Pain related behaviour in two models of osteoarthritis in the rat knee. Pain. 112(1-2):83-93.
  • 16. ElBatsh MM, Eledel R, Noreldin RI, Mohamed ASED, Omar TA (2021). Effect of Intra-Articular Injection of Chondrocytes Differentiated from Mesenchymal Stem Cells in Monosodium iodoacetate Induced Osteoarthritis in Male Rats. Bulletin of Egyptian Society for Physiological Sciences, 41(2), 144-156.
  • 17. M Halfaya F, GH R., OM A., WA E (2020). Efficacy of Hyaluronic Acid in the Treatment of MIA-induced Ankle Osteoarthritis in Rats and its Effect on Antioxidant Response Element. Journal of Veterinary Medical Research.
  • 18. Gotoh S, Onaya J, Abe M, et al. (1993). Effects of the molecular weight of hyaluronic acid and its action mechanisms on experimental joint pain in rats. Ann Rheum Dis. 52(11):817-822.
  • 19. Neugebauer V, Han JS, Adwanikar H, Fu Y, Ji G (2007). Techniques for assessing knee joint pain in arthritis. Molecular Pain.;3,1744-8069.
  • 20. Filardo G, Kon E, Di Martino A, et al. (2012). Platelet-rich plasma vs hyaluronic acid to treat knee degenerative pathology: study design and preliminary results of a randomized controlled trial. BMC Musculoskelet Disord. 13(1):229.
  • 21. Morais SVD, Czeczko NG, Malafaia O, et al (2016). Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee. Acta Cir Bras. 31(11):765-773.
  • 22. Teeple E, Elsaid KA, Jay GD, et al. (2011). Effects of supplemental intra-articular lubricin and hyaluronic acid on the progression of posttraumatic arthritis in the anterior cruciate ligament–deficient rat knee. Am J Sports Med. 39(1):164-172.
  • 23. Kang SW, Bada LP, Kang CS, et al. (2008). Articular cartilage regeneration with microfracture and hyaluronic acid. Biotechnology letters 30(3):435-439.
  • 24. Strauss E, Schachter A, Frenkel S, Rosen J (2009). The efficacy of intra-articular hyaluronan injection after the microfracture technique for the treatment of articular cartilage lesions. Am J Sports Med. 37(4):720-726.
  • 25. Gunes T, Bostan B, Erdem M, Koseoglu RD, Asci M, Sen C (2012). Intraarticular hyaluronic acid injection after microfracture technique for the management of full-thickness cartilage defects does not improve the quality of repair tissue. Cartilage :3(1):20-26.

Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats

Year 2021, Volume: 14 Issue: 1, 56 - 61, 30.06.2021
https://doi.org/10.47027/duvetfd.886960

Abstract

The animals with osteoarthritis suffer from clinical signs such as joint pain, reluctance to move, loss of performance and lameness. Many medical and interventional methods are used to manage osteoarthritis but no described treatment can completely repair damaged cartilage yet. In this study, 40 male Wistar Albino rats were randomly assigned as microfracture technique group, intra-articular hyaluronic acid group, microfracture technique + intra-articular hyaluronic acid group and control group (n=10). Clinically, knee circumference and body weight were measured and, leg posture was scored. Radiological examination findings were evaluated using a grading based on 3 parameter as joint space narrowing, subchondral bone sclerosis, osteophyte formation. Also, at the end of the experiment, following sacrification, the joint of the relevant leg was scored according to the severity of osteoarthritis. Considering the changes in the leg posture, the knee circumference measurement and the radiological findings, the development of osteoarthritis was successfully achieved with monosodium iodoacetate application in this study. In line with the findings obtained from the presented study, it was concluded that combining microfracture technique with hyaluronic acid application would not contribute additionally to the treatment process, and even using microfracture or hyaluronic acid application alone may produce more positive results.

Project Number

VTF-16019

References

  • 1. Kikuchi T, Yamada H, Shimmei M (1996). Effect of high molecular weight hyaluronan on cartilage degeneration in a rabbit model of osteoarthritis. Osteoarthr Cartil.4(2):99-110.
  • 2. He Y, Li Z, Alexander PG, et al. (2020). Pathogenesis of osteoarthritis: risk factors, regulatory pathways in chondrocytes, and experimental models. Biology 9(8): 194.
  • 3. Moskowitz RW, Altman RD, Hochberg MC, Buckwalter JA, Goldberg VM (2007). Osteoarthritis: Diagnosis and medical/surgical management. 4th ed., Lippincott, Williams & Wilkins, USA.
  • 4. Kerwin SC (2010). Osteoarthritis in cats. Top Companion Anim Med. 25(4):218-23.
  • 5. Henrotin Y, Sanchez C, Balligand M. (2005) Pharmaceutical and nutraceutical management of canine osteoarthritis: present and future perspectives. Vet J. 170(1):113-23.
  • 6. Goodrich LR, Nixon AJ (2006). Medical treatment of osteoarthritis in the horse – a review. Vet J 171(1):51-69.
  • 7. Rychel JK (2010). Diagnosis and Treatment of Osteoarthritis, Top Companion Anim Med. 25(1):20-25.
  • 8. Steadman J, Rodkey W, Briggs K, Rodrigo J (1999). The microfracture technic in the management of complete cartilage defects in the knee joint. Orthopade. 28:26–32.
  • 9. Sluka KA, Milton MA, Willis WD, Westlund KN (1997). Differential roles of neurokinin 1 and neurokinin 2 receptors in the development and maintenance of heat hyperalgesia induced by acute inflammation Br J Pharmacol. 120(7):1263–1273.
  • 10. Evans RG, Collins C, Miller P, Ponsford FM, Elson CJ (1994). Radiological scoring of osteoarthritis progression in STR/ORT mice. Osteoarthr Cartil.2:103–9.
  • 11. Bennett GA, Waine H, Bauer W (1942). Changes in the knee joint at various ages with particular reference to the nature and development of degenerative joint disease. New York: The Commonwealth Fund.1-93.
  • 12. Clarke KA, Heitmeyer SA, Smith AG, Taiwo YO (1997). Gait analysis in a rat model of osteoarthrosis. Physiol Behav. 62(5):951-954.
  • 13. Xu J, Yan L, Yan B, et al. (2020). Osteoarthritis Pain Model Induced by Intra-Articular Injection of Mono-Iodoacetate in Rats. JoVE (Journal of Visualized Experiments), (159), e60649.
  • 14. Rebai MA, Sahnoun N, Abdelhedi et al. (2020) Animal models of osteoarthritis: characterization of a model induced by Mono-Iodo-Acetate injected in rabbits. Libyan Journal of Medicine, 15(1): 1753943.
  • 15. Fernihough J, Gentry C, Malcangio M, et al. (2004). Pain related behaviour in two models of osteoarthritis in the rat knee. Pain. 112(1-2):83-93.
  • 16. ElBatsh MM, Eledel R, Noreldin RI, Mohamed ASED, Omar TA (2021). Effect of Intra-Articular Injection of Chondrocytes Differentiated from Mesenchymal Stem Cells in Monosodium iodoacetate Induced Osteoarthritis in Male Rats. Bulletin of Egyptian Society for Physiological Sciences, 41(2), 144-156.
  • 17. M Halfaya F, GH R., OM A., WA E (2020). Efficacy of Hyaluronic Acid in the Treatment of MIA-induced Ankle Osteoarthritis in Rats and its Effect on Antioxidant Response Element. Journal of Veterinary Medical Research.
  • 18. Gotoh S, Onaya J, Abe M, et al. (1993). Effects of the molecular weight of hyaluronic acid and its action mechanisms on experimental joint pain in rats. Ann Rheum Dis. 52(11):817-822.
  • 19. Neugebauer V, Han JS, Adwanikar H, Fu Y, Ji G (2007). Techniques for assessing knee joint pain in arthritis. Molecular Pain.;3,1744-8069.
  • 20. Filardo G, Kon E, Di Martino A, et al. (2012). Platelet-rich plasma vs hyaluronic acid to treat knee degenerative pathology: study design and preliminary results of a randomized controlled trial. BMC Musculoskelet Disord. 13(1):229.
  • 21. Morais SVD, Czeczko NG, Malafaia O, et al (2016). Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee. Acta Cir Bras. 31(11):765-773.
  • 22. Teeple E, Elsaid KA, Jay GD, et al. (2011). Effects of supplemental intra-articular lubricin and hyaluronic acid on the progression of posttraumatic arthritis in the anterior cruciate ligament–deficient rat knee. Am J Sports Med. 39(1):164-172.
  • 23. Kang SW, Bada LP, Kang CS, et al. (2008). Articular cartilage regeneration with microfracture and hyaluronic acid. Biotechnology letters 30(3):435-439.
  • 24. Strauss E, Schachter A, Frenkel S, Rosen J (2009). The efficacy of intra-articular hyaluronan injection after the microfracture technique for the treatment of articular cartilage lesions. Am J Sports Med. 37(4):720-726.
  • 25. Gunes T, Bostan B, Erdem M, Koseoglu RD, Asci M, Sen C (2012). Intraarticular hyaluronic acid injection after microfracture technique for the management of full-thickness cartilage defects does not improve the quality of repair tissue. Cartilage :3(1):20-26.
There are 25 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Umut Arda 0000-0001-6692-0318

Zeynep Bozkan 0000-0003-4233-6496

Project Number VTF-16019
Publication Date June 30, 2021
Acceptance Date April 29, 2021
Published in Issue Year 2021 Volume: 14 Issue: 1

Cite

APA Arda, U., & Bozkan, Z. (2021). Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 14(1), 56-61. https://doi.org/10.47027/duvetfd.886960
AMA Arda U, Bozkan Z. Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats. Dicle Üniv Vet Fak Derg. June 2021;14(1):56-61. doi:10.47027/duvetfd.886960
Chicago Arda, Umut, and Zeynep Bozkan. “Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14, no. 1 (June 2021): 56-61. https://doi.org/10.47027/duvetfd.886960.
EndNote Arda U, Bozkan Z (June 1, 2021) Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14 1 56–61.
IEEE U. Arda and Z. Bozkan, “Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats”, Dicle Üniv Vet Fak Derg, vol. 14, no. 1, pp. 56–61, 2021, doi: 10.47027/duvetfd.886960.
ISNAD Arda, Umut - Bozkan, Zeynep. “Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14/1 (June 2021), 56-61. https://doi.org/10.47027/duvetfd.886960.
JAMA Arda U, Bozkan Z. Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats. Dicle Üniv Vet Fak Derg. 2021;14:56–61.
MLA Arda, Umut and Zeynep Bozkan. “Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 14, no. 1, 2021, pp. 56-61, doi:10.47027/duvetfd.886960.
Vancouver Arda U, Bozkan Z. Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats. Dicle Üniv Vet Fak Derg. 2021;14(1):56-61.