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Silybum marianum L. Gaertner'in Antioksidan Kapasitesi, Toplam Fenolik ve Flavonoid İçerikleri

Year 2022, Issue: 43, 17 - 20, 30.11.2022
https://doi.org/10.31590/ejosat.1206557

Abstract

Terapötik özelliklerinden dolayı Silybum marianum'un toprak üztünde kalan kısımları geleneksel olarak halk hekimliğinde kullanılmaktır. Bu çalışmanın amacı, Silybum marianum toprak üstü kısımlarının metanol ekstraktlarının antioksidan kapasitesini ve toplam fenol ve flavonoid içeriklerini belirlemektir. Silybum marianum'un antioksidan kapasitesini belirlemek için DPPH (2, 2-difenil-1-pikrilhidrazil) serbest radikal süpürme ve indirgeme kapasitesi deneyleri kullanıldı. Test edilen tüm ekstraktlar, önemli ölçüde toplam fenolik ve toplam flavonoid içeriğinin yanı sıra güçlü antioksidan kapasite göstermiştir. Metanolik ekstraktın, bir miktar fenol (kurutulmuş materyalin gramı başına 56.95±0.78 µmg gallik asit eşdeğeri) ve flavonoidler (ekstrenin gramı başına 86.09±0.35 µmg kersetin eşdeğeri) içerdiği bulundu. Ayrıca aynı ekstraktın önemli ölçüde daha yüksek DPPH indirgeme gücüne ve süpürme kapasitesine (İnhibisyon % =  91.77±0.12) sahip olduğu gözlenmiştir. Bu bulgular, Silybum marianum toprak üstünde kalan kısımlarının gıda, ilaç ve kozmetik endüstrilerinde doğal bir antioksidan kaynağı olarak kullanılabileceğini göstermektedir.

References

  • Al‐Anati, L., Essid, E., Reinehr, R., & Petzinger, E. (2009). Silibinin protects OTA‐mediated TNF‐α release from perfused rat livers and isolated rat Kupffer cells. Molecular nutrition & food research, 53(4), 460-466.
  • Jayaraj, R., Deb, U., Bhaskar, A. S. B., Prasad, G. B. K. S., & Rao, P. L. (2007). Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice. Environmental Toxicology: An International Journal, 22(5), 472-479.
  • Fehér, J., Láng, I., Deák, G., Cornides, A., Nékám, K., & Gergely, P. (1986). Free radicals in tissue damage in liver diseases and therapeutic approach. The Tokai Journal of Experimental and Clinical Medicine, 11, 121-134.
  • Křen, V., Kubisch, J., Sedmera, P., Halada, P., Přikrylová, V., Jegorov, A., ... & Šimánek, V. (1997). Glycosylation of silybin. Journal of the Chemical Society, Perkin Transactions 1, (17), 2467-2474.
  • Mira, L., Silva, M., & Manso, C. F. (1994). Scavenging of reactive oxygen species by silibinin dihemisuccinate. Biochemical pharmacology, 48(4), 753-759.
  • Škottová, N., Krečman, V., & Šimánek, V. (1999). Activities of silymarin and its flavonolignans upon low density lipoprotein oxidizability in vitro. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 13(6), 535-537.
  • Miguez, M. P., Anundi, I., Sainz-Pardo, L. A., & Lindros, K. O. (1994). Hepatoprotective mechanism of silymarin: no evidence for involvement of cytochrome P450 2E1. Chemico-biological interactions, 91(1), 51-63.
  • Miller, A. L. (1996). Antioxidant flavonoids: structure, function and clinical usage. Alt Med Rev, 1(2), 103-111.
  • Wiseman, H. (1996). Dietary influences on membrane function: importance in protection against oxidative damage and disease. The Journal of Nutritional Biochemistry, 7(1), 2-15.
  • Muriel, P., & Mourelle, M. (1990). Prevention by silymarin of membrane alterations in acute CCI4 liver damage. Journal of Applied Toxicology, 10(4), 275-279.
  • Lucini, L., Pellizzoni, M., Pellegrino, R., Molinari, G. P., & Colla, G. (2015). Phytochemical constituents and in vitro radical scavenging activity of different Aloe species. Food chemistry, 170, 501-507.
  • Colla, G., Rouphael, Y., Cardarelli, M., Svecova, E., Rea, E., & Lucini, L. (2013). Effects of saline stress on mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon genotypes grown in floating system. Journal of the Science of Food and Agriculture, 93(5), 1119-1127.
  • MA, E., Hosseinimehr, S. J., Hamidinia, A., & Jafari, M. (2008). Antioxidant and free radical scavenging activity of Feijoa sellowiana fruits peel and leaves. Pharmacologyonline, 1, 7-14.

Total phenol and flavonoid contents, and antioxidant capacity of Silybum marianum L. Gaertner grown in Turkey

Year 2022, Issue: 43, 17 - 20, 30.11.2022
https://doi.org/10.31590/ejosat.1206557

Abstract

Because of their therapeutic properties, the aerial parts of Silybum marianum have traditionally been used in folk medicine. The purpose of this study was to determine the antioxidant capacity and total phenol and flavonoid contents of methanol extracts of Silybum marianum aerial parts. DPPH (2, 2-diphenyl-1-picrylhydrazil) free radical scavenging and reducing power assays were used to assess the antioxidant capacity of Silybum marianum. The total phenol and flavonoid contents of each extract were determined using the Folin-Ciocalteu and aluminum trichloride methods, respectively. DPPH (2, 2-diphenyl-1-picrylhydrazil) free radical scavenging assays were used to assess the antioxidant capacity of Silybum marianum extracts. All of the extracts tested showed impressive total phenolic and total flavonoid content, as well as potent antioxidant capacity. The methanolic extract was found to be containing a number of total phenols (56.95±0.78  mg of gallic acid equivalent per gram of dried material) and flavonoids (86.09±0.35 mg of quercetin equivalent per gram of extract). The same extract also had a significantly higher DPPH-reducing power and scavenging capacity (Inhibition % =  91.77±0.12). These findings imply that Silybum marianum aerial parts could be used as a natural source of antioxidants in the food, pharmaceutical, and cosmetic industries.

References

  • Al‐Anati, L., Essid, E., Reinehr, R., & Petzinger, E. (2009). Silibinin protects OTA‐mediated TNF‐α release from perfused rat livers and isolated rat Kupffer cells. Molecular nutrition & food research, 53(4), 460-466.
  • Jayaraj, R., Deb, U., Bhaskar, A. S. B., Prasad, G. B. K. S., & Rao, P. L. (2007). Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice. Environmental Toxicology: An International Journal, 22(5), 472-479.
  • Fehér, J., Láng, I., Deák, G., Cornides, A., Nékám, K., & Gergely, P. (1986). Free radicals in tissue damage in liver diseases and therapeutic approach. The Tokai Journal of Experimental and Clinical Medicine, 11, 121-134.
  • Křen, V., Kubisch, J., Sedmera, P., Halada, P., Přikrylová, V., Jegorov, A., ... & Šimánek, V. (1997). Glycosylation of silybin. Journal of the Chemical Society, Perkin Transactions 1, (17), 2467-2474.
  • Mira, L., Silva, M., & Manso, C. F. (1994). Scavenging of reactive oxygen species by silibinin dihemisuccinate. Biochemical pharmacology, 48(4), 753-759.
  • Škottová, N., Krečman, V., & Šimánek, V. (1999). Activities of silymarin and its flavonolignans upon low density lipoprotein oxidizability in vitro. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 13(6), 535-537.
  • Miguez, M. P., Anundi, I., Sainz-Pardo, L. A., & Lindros, K. O. (1994). Hepatoprotective mechanism of silymarin: no evidence for involvement of cytochrome P450 2E1. Chemico-biological interactions, 91(1), 51-63.
  • Miller, A. L. (1996). Antioxidant flavonoids: structure, function and clinical usage. Alt Med Rev, 1(2), 103-111.
  • Wiseman, H. (1996). Dietary influences on membrane function: importance in protection against oxidative damage and disease. The Journal of Nutritional Biochemistry, 7(1), 2-15.
  • Muriel, P., & Mourelle, M. (1990). Prevention by silymarin of membrane alterations in acute CCI4 liver damage. Journal of Applied Toxicology, 10(4), 275-279.
  • Lucini, L., Pellizzoni, M., Pellegrino, R., Molinari, G. P., & Colla, G. (2015). Phytochemical constituents and in vitro radical scavenging activity of different Aloe species. Food chemistry, 170, 501-507.
  • Colla, G., Rouphael, Y., Cardarelli, M., Svecova, E., Rea, E., & Lucini, L. (2013). Effects of saline stress on mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon genotypes grown in floating system. Journal of the Science of Food and Agriculture, 93(5), 1119-1127.
  • MA, E., Hosseinimehr, S. J., Hamidinia, A., & Jafari, M. (2008). Antioxidant and free radical scavenging activity of Feijoa sellowiana fruits peel and leaves. Pharmacologyonline, 1, 7-14.
There are 13 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Erten Akbel 0000-0002-6954-3658

İbrahim Bulduk 0000-0001-6172-7738

Early Pub Date November 25, 2022
Publication Date November 30, 2022
Published in Issue Year 2022 Issue: 43

Cite

APA Akbel, E., & Bulduk, İ. (2022). Silybum marianum L. Gaertner’in Antioksidan Kapasitesi, Toplam Fenolik ve Flavonoid İçerikleri. Avrupa Bilim Ve Teknoloji Dergisi(43), 17-20. https://doi.org/10.31590/ejosat.1206557