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İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci

Year 2016, Volume: 13 Issue: 2, 139 - 150, 04.08.2016

Abstract

 Bu çalışmada çiğ inek sütlerinden Enterococcus spp’lerin izolasyonu ve identifikasyonu ile elde edilen izolatlarda
vankomisin direncinin fenotipik ve moleküler yöntemlerle saptanması amaçlandı. Toplam 150 adet süt örneği sağlıklı
ve mastitisli hayvanlardan California Mastitis Test ile skorlanarak toplandı. Seksen dört Enterecoccus spp. izolatı
elde edildi ve bunların 57 (%68), 8 (%9) ve 19 (%23)’ü sırasıyla Enterococcus faecalis, Enterococcus faecium and
Enterococcus spp. olarak polimeraz zincir reaksiyonu ile identifiye edildi. Etest ile izolatların hiçbiri vankomisine
direnç göstermedi. Bununla birlikte, E. faecalis izolatlarının 11 (%19)’inde ve E. faecium izolatlarının 7(%88)’sinde
VanB geni pozitif olarak tespit edildi. Ayrıca, 1 izolatta VanC2, VanC3 genleri ve 2 E. faecium izolatında VanB, VanC2,
VanC3 genleri birlikte bulundu. Bu çalışmada inek sütlerinde önemli derecede Enterococcus spp. varlığı saptanmıştır.
Vankomisin direncinin moleküler testlerle tespitinden dolayı bu test, antibiyotik direncin tespitinde daha etkili olduğu
sonucuna varıldı

References

  • 1. Araya M, Davidovich G, Arias ML, Chaves C. Identification of Enterococcus sp. isolated from raw milk samples coming from the metropolitan area of Costa Rica and evaluation of its antibiotic sensibility pattern. Arch Latinoam Nutr 2005; 55(2): 161-6.
  • 2. Butaye P, Devriese LA, Haesebrouck F. Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on Gram-positive bacteria. Clin Microbiol Rev 2003; 16(2): 175-88.
  • 3. Cenci Goga BT, Aquilanti L, Osimani A, Miraqlia D, Aloisio F. Identification with multiplex PCR assay of Enterococcus species isolated from dairy products in Umbria, Italy. Vet Res Commun 2003; 27(1): 667-71.
  • 4. Choi SS, Kim BS, Ha NJ. Isolation, identification and characterization of vancomycin-resistant enterococci from raw milk. J Microbiology 2002; 40(2): 170-2.
  • 5. CLSI. Performance standards for antimicrobial susceptibility testing: 20th informational supplement (M100-S20). CLSI, Clinical and Laboratory Standards Institute, Wayne. 2010.
  • 6. Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F. Identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows. Vet Microbiol 1999; 70(1-2): 87-94.
  • 7. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptides resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995; 33(5): 24-7 (Erratum, 33:1434).
  • 8. Franciosi E, Settanni L, Cavazza A, Poznanski E. Presence of enterococci in raw cow’s milk and “Puzzone di Moena” cheese. J Food Process Press 2009; 33(2): 204-17.
  • 9. Janoskova A, Kmet V. Vancomycin resistance genes in Enterococcus spp. strains isolated from alpine accentor and chamois. Acta Veterinaria Brno 2004; 73(2): 211-4.
  • 10. Jung WK, Lim JY, Kwon NH, Kim JM, Hong SK, Koo HC, Kim SH, Park YH. Vancomycin resistant enterococci from animal sources in Korea. Int J Food Microbiol 2007; 113(1): 102-7.
  • 11. Kasikci G, Cetin O, Bingol EB, Gunduz MC. Relations between electrical conductivity, somatic cell count, California mastitis test and some quality parameters in the diagnosis of subclinical mastitis in dairy cows. Turk J Vet Anim Sci 2012; 36(1): 49-55.
  • 12. Kateete DP, Kabugo U, Baluku H, Nyakarahuka L, Kyobe S, Okee M, Najjuka CF, Joloba ML. Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda. PLoS ONE 2013; 8(5); e63413.
  • 13. Kühn I, Iversen A, Finn M, Greko C, Burman LG, Blanch AR, Vilanova X, Manero A, Taylor H, Caplin J, Dominquez L, Herrerp IA, Moreno MA, Möllby R. Occurrence and relatedness of vancomycinresistant enterococci in animals, humans, and the environment in different European regions. Appl Environ Microbiol 2005; 71(9): 5383-90.
  • 14. Kuyucuoglu Y, Antibiotic resistance of enterococci isolated from bovine subclinical mastitis. Eurasian J Vet Sci 2011; 27(4): 231-4.
  • 15. Li S, Zhan Z, Mi ZH. Vancomycin resistant enterococci in a Chinese hospital. Curr Microbiol 2007; 55(2): 125-7.
  • 16. Lukasova J, Sustackova A. Enterococci and antibiotic resistance. Acta Veterinaria Brno 2003; 72: 315-23.
  • 17. Mannu L, Paba A, Daga E, Comunian R, Zanetti S, Dupre I, Sechi LA. Comparison of incidence of virulence determinants and antibiotics resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int J Food Microbiol 2003; 88(2-3): 291- 304.
  • 18. Marshall BM, Levy SB. Food animals and antimicrobials: impacts on human health. Clin Microbiol Rev 2011; 24(4): 718-33.
  • 19. Nam HM, Lim SK, Moon JS, Kang HM, Kim JM, Jang KC, Kim JM, Kang MI, Joo YS, Jung SC. Antimicrobial resistance of enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses Public Health 2010; 57(7-8): 59-64.
  • 20. Nilsson O. Vancomycin resistant enterococci in farm animals-occurrence importance. Infect Ecol Epidemiol 2012; 2: e16959.
  • 21. Petersson-Wolfe CS. A study of the occurrence, phenotypic and genotypic diversity and both in vitro and in vivo growth response of Enterococcus spp. isolated from bovine origin, Degree Doctor of Philosophy, The Ohio State University, USA 2006; pp. 12-5.
  • 22. Pitkala A, Haveri M, Pyorala S, Myllys V, Honkanen-Buzalski T. Bovine mastitis in Finland 2001-prevalence, distribution of bacteria, and antimicrobial resistance. J Dairy Sci 2004; 87(8): 2433-41.
  • 23. Quinn PJ, Carter ME, Markey B, Carter GR. The Streptococci and related cocci species. Quinn PJ, Carter ME, Markey B, Carter GR. eds. In: Clinical Veterinary Microbiology. London: Mosby, 1998; pp. 127-36.
  • 24. Riboldi GP, Frazzon J, D’azevedo PA, Frazzon APG. Antimicrobial resistance profile of Enterococcus spp. isolated from food in Southern Brazil. Braz J Microbiol 2009; 40(1): 125-8.
  • 25. Schleifer KH, Balz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Bacteriol 1984; 34(1): 31-4.
  • 26. Trivedi K, Cupakova S, Karpiskova R. Virulence factors and antibiotic resistance in enterococci isolated from food-stuffs. Vet Med Czech 2011; 56(7): 352-7.
  • 27. Uttley AH, Collins CH, Naidoo J, George RC. Vancomycin resistant enterococci. Lancet 1988; 1: 57-8.
  • 28. Winn WJr, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, Woods G. Koneman’s color atlas and textbook of diagnostic microbiology. Sixth Edition. Baltimore, MD: Lippincott Williams & Wilkins. 2006; pp. 673-764.
  • 29. Yildirim Z, Bilgin H, Isleroglu H, TokatliK, Sahingil D, Yildirim M. Enterocin HZ produced by a wild Enterococcus faecium strain isolated from a traditional, starterfree pickled cheese. J Dairy Res 2014; 81(2): 164-72.
  • 30. Zirakzadeh A, Patel R. Vancomycin resistant enterococci: Colonization, infection, detection and treatment. Mayo Clin Proc 2006; 81(4): 529-36.

Vancomycin Resistance of Enterococcus faecalis and Enterococcus faecium Isolated from cattle milk

Year 2016, Volume: 13 Issue: 2, 139 - 150, 04.08.2016

Abstract

In this study the presence of Enterococcus spp. in raw cattle milk and the detection of the resistance to
vancomycin of the isolates by using phenotypic and molecular methods were investigated. Totally, 150 milk samples
were collected from healthy animals or animals with mastitis scored with California Mastitis Test. Eighty four
Enterococcus spp. were isolated and 57 (68%), 8 (9%) and 19 (23%) of the isolates were identified by Polymerase
Chain Reaction as Enterococcus faecalis, Enterococcus faecium and Enterococcus spp., respectively. None of the
isolates were resistant to vancomycin with E test. However, 11 (19%) E. faecalis and 7 (88%) E. faecium isolates
were positive for VanB. VanC2, VanC3 and VanB, VanC2, VanC3 genes were found together in 1 and 2 E. faecium
isolates, respectively. In this study, Enterococcus spp. were significantly found in cattle milk. Because of the detection
of vancomycin resistance by molecular test, this method was found to be more effective in the detection of antibiotic
resistance.

References

  • 1. Araya M, Davidovich G, Arias ML, Chaves C. Identification of Enterococcus sp. isolated from raw milk samples coming from the metropolitan area of Costa Rica and evaluation of its antibiotic sensibility pattern. Arch Latinoam Nutr 2005; 55(2): 161-6.
  • 2. Butaye P, Devriese LA, Haesebrouck F. Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on Gram-positive bacteria. Clin Microbiol Rev 2003; 16(2): 175-88.
  • 3. Cenci Goga BT, Aquilanti L, Osimani A, Miraqlia D, Aloisio F. Identification with multiplex PCR assay of Enterococcus species isolated from dairy products in Umbria, Italy. Vet Res Commun 2003; 27(1): 667-71.
  • 4. Choi SS, Kim BS, Ha NJ. Isolation, identification and characterization of vancomycin-resistant enterococci from raw milk. J Microbiology 2002; 40(2): 170-2.
  • 5. CLSI. Performance standards for antimicrobial susceptibility testing: 20th informational supplement (M100-S20). CLSI, Clinical and Laboratory Standards Institute, Wayne. 2010.
  • 6. Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F. Identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows. Vet Microbiol 1999; 70(1-2): 87-94.
  • 7. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptides resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995; 33(5): 24-7 (Erratum, 33:1434).
  • 8. Franciosi E, Settanni L, Cavazza A, Poznanski E. Presence of enterococci in raw cow’s milk and “Puzzone di Moena” cheese. J Food Process Press 2009; 33(2): 204-17.
  • 9. Janoskova A, Kmet V. Vancomycin resistance genes in Enterococcus spp. strains isolated from alpine accentor and chamois. Acta Veterinaria Brno 2004; 73(2): 211-4.
  • 10. Jung WK, Lim JY, Kwon NH, Kim JM, Hong SK, Koo HC, Kim SH, Park YH. Vancomycin resistant enterococci from animal sources in Korea. Int J Food Microbiol 2007; 113(1): 102-7.
  • 11. Kasikci G, Cetin O, Bingol EB, Gunduz MC. Relations between electrical conductivity, somatic cell count, California mastitis test and some quality parameters in the diagnosis of subclinical mastitis in dairy cows. Turk J Vet Anim Sci 2012; 36(1): 49-55.
  • 12. Kateete DP, Kabugo U, Baluku H, Nyakarahuka L, Kyobe S, Okee M, Najjuka CF, Joloba ML. Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda. PLoS ONE 2013; 8(5); e63413.
  • 13. Kühn I, Iversen A, Finn M, Greko C, Burman LG, Blanch AR, Vilanova X, Manero A, Taylor H, Caplin J, Dominquez L, Herrerp IA, Moreno MA, Möllby R. Occurrence and relatedness of vancomycinresistant enterococci in animals, humans, and the environment in different European regions. Appl Environ Microbiol 2005; 71(9): 5383-90.
  • 14. Kuyucuoglu Y, Antibiotic resistance of enterococci isolated from bovine subclinical mastitis. Eurasian J Vet Sci 2011; 27(4): 231-4.
  • 15. Li S, Zhan Z, Mi ZH. Vancomycin resistant enterococci in a Chinese hospital. Curr Microbiol 2007; 55(2): 125-7.
  • 16. Lukasova J, Sustackova A. Enterococci and antibiotic resistance. Acta Veterinaria Brno 2003; 72: 315-23.
  • 17. Mannu L, Paba A, Daga E, Comunian R, Zanetti S, Dupre I, Sechi LA. Comparison of incidence of virulence determinants and antibiotics resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int J Food Microbiol 2003; 88(2-3): 291- 304.
  • 18. Marshall BM, Levy SB. Food animals and antimicrobials: impacts on human health. Clin Microbiol Rev 2011; 24(4): 718-33.
  • 19. Nam HM, Lim SK, Moon JS, Kang HM, Kim JM, Jang KC, Kim JM, Kang MI, Joo YS, Jung SC. Antimicrobial resistance of enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses Public Health 2010; 57(7-8): 59-64.
  • 20. Nilsson O. Vancomycin resistant enterococci in farm animals-occurrence importance. Infect Ecol Epidemiol 2012; 2: e16959.
  • 21. Petersson-Wolfe CS. A study of the occurrence, phenotypic and genotypic diversity and both in vitro and in vivo growth response of Enterococcus spp. isolated from bovine origin, Degree Doctor of Philosophy, The Ohio State University, USA 2006; pp. 12-5.
  • 22. Pitkala A, Haveri M, Pyorala S, Myllys V, Honkanen-Buzalski T. Bovine mastitis in Finland 2001-prevalence, distribution of bacteria, and antimicrobial resistance. J Dairy Sci 2004; 87(8): 2433-41.
  • 23. Quinn PJ, Carter ME, Markey B, Carter GR. The Streptococci and related cocci species. Quinn PJ, Carter ME, Markey B, Carter GR. eds. In: Clinical Veterinary Microbiology. London: Mosby, 1998; pp. 127-36.
  • 24. Riboldi GP, Frazzon J, D’azevedo PA, Frazzon APG. Antimicrobial resistance profile of Enterococcus spp. isolated from food in Southern Brazil. Braz J Microbiol 2009; 40(1): 125-8.
  • 25. Schleifer KH, Balz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Bacteriol 1984; 34(1): 31-4.
  • 26. Trivedi K, Cupakova S, Karpiskova R. Virulence factors and antibiotic resistance in enterococci isolated from food-stuffs. Vet Med Czech 2011; 56(7): 352-7.
  • 27. Uttley AH, Collins CH, Naidoo J, George RC. Vancomycin resistant enterococci. Lancet 1988; 1: 57-8.
  • 28. Winn WJr, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, Woods G. Koneman’s color atlas and textbook of diagnostic microbiology. Sixth Edition. Baltimore, MD: Lippincott Williams & Wilkins. 2006; pp. 673-764.
  • 29. Yildirim Z, Bilgin H, Isleroglu H, TokatliK, Sahingil D, Yildirim M. Enterocin HZ produced by a wild Enterococcus faecium strain isolated from a traditional, starterfree pickled cheese. J Dairy Res 2014; 81(2): 164-72.
  • 30. Zirakzadeh A, Patel R. Vancomycin resistant enterococci: Colonization, infection, detection and treatment. Mayo Clin Proc 2006; 81(4): 529-36.
There are 30 citations in total.

Details

Journal Section Articles
Authors

Tekin Kececı This is me

Kadir Semih Gumussoy This is me

Harun Hızlısoy

Publication Date August 4, 2016
Submission Date December 6, 2016
Acceptance Date August 2, 2016
Published in Issue Year 2016 Volume: 13 Issue: 2

Cite

APA Kececı, T., Gumussoy, K. S., & Hızlısoy, H. (2016). İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 13(2), 139-150.
AMA Kececı T, Gumussoy KS, Hızlısoy H. İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci. Erciyes Üniv Vet Fak Derg. August 2016;13(2):139-150.
Chicago Kececı, Tekin, Kadir Semih Gumussoy, and Harun Hızlısoy. “İnek Sütlerinden İzole Edilen Enterococcus Faecalis Ve Enterococcus faecium’un Vankomisin Direnci”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13, no. 2 (August 2016): 139-50.
EndNote Kececı T, Gumussoy KS, Hızlısoy H (August 1, 2016) İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13 2 139–150.
IEEE T. Kececı, K. S. Gumussoy, and H. Hızlısoy, “İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci”, Erciyes Üniv Vet Fak Derg, vol. 13, no. 2, pp. 139–150, 2016.
ISNAD Kececı, Tekin et al. “İnek Sütlerinden İzole Edilen Enterococcus Faecalis Ve Enterococcus faecium’un Vankomisin Direnci”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13/2 (August 2016), 139-150.
JAMA Kececı T, Gumussoy KS, Hızlısoy H. İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci. Erciyes Üniv Vet Fak Derg. 2016;13:139–150.
MLA Kececı, Tekin et al. “İnek Sütlerinden İzole Edilen Enterococcus Faecalis Ve Enterococcus faecium’un Vankomisin Direnci”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 13, no. 2, 2016, pp. 139-50.
Vancouver Kececı T, Gumussoy KS, Hızlısoy H. İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci. Erciyes Üniv Vet Fak Derg. 2016;13(2):139-50.