Research Article
Histological and Immunohistochemical Examination in Testicular Tissue of Diabetic Rats Treated with Dandelion (Taraxacum officinale) Extract
Abstract
The aim of this study was to evaluate the changes induced in testicular tissue by administration of dandelion (Taraxacum officinale) extract and its effects on the release of enzymatic antioxidants SOD-2 by immunohistochemical methods in rats with experimental diabetes. Degenerative changes in the seminiferous tubule structure were observed in the testicular sections of the diabetes group. Separations between the basement membrane and the seminiferous epithelium, disruptions in the structure and localization of spermatogenic cells toward the lumen of the seminiferous tubules, thickening of the basement membrane and tunica albuginea, changes in the shape of Sertoli cells, and shrinkage of the nucleus of Leydig cells were observed. While no immunopositivity was observed in spermatogonium and primary spermatocytes in the diabetes group, weak SOD-2 immunoreactivity was observed in other cells of the spermatogenic series and moderate SOD-2 immunoreactivity was observed in the diabetes+dandelion group. In conclu-sion, it was found that histopathological changes occurred in the testicular tissue and SOD-2 immunoreactivity decreased in diabetes, a chronic metabolic disease. It was concluded that dandelion administration may have beneficial effects on decreased SOD-2 immunoreactivity.
Keywords
References
- Al-Salmi AF, Hamza RZ. Efficacy of vanadyl sulfate and selenium tetrachloride as anti-diabetic agents against hyperglycemia and oxidative stress induced by diabetes mellitus in male rats. Curr Is-sues Mol Biol 2022; 44 (1): 94-104.
- Bener A, Al-Ansari AA, Zirie M, Al-Hamaq AO. Is male fertility associated with type 2 diabetes melli-tus? Int Urol Nephrol 2009; 41(4): 777-84.
- Choi UK, Lee OH, Yim JH, Cho CW, Rhee YK, Lim SI, Kim YC. Hypolipidemic and antioxidant effects of dandelion (taraxacum officinale) root and leaf on cholesterol-fed rabbits. Int J Mol Sci 2010; 11(1): 67-78.
- Dongmei C. Atlas of Histology with Functional and Clinical Correlations. First Edition. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wil-kins, 2011; p.344-68.
- Donmez YB, Kizilay G, Topcu-Tarladacalisir Y. MAPK immunoreactivity in streptozotocin-induced diabetic rat testis. Acta Cir Bras 2014; 29(10): 644-50.
- Ersoy O, Kizilay G. Effects of fucoidan on diabetic rat testicular tissue. Biotech Histochem 2018; 93(4): 277-85.
- Fridovich I. Superoxide dismutases. Annu Rev Bio-chem 1975; 44: 147-59.
- Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cil-aker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res 2008; 40(4): 354-60.
- International Diabetes Federation, Diabetes atlas 9th edition. https://www.diabetesatlas.org/en/sections/worldwide-toll-of-diabetes.html. 2019. Access: 24.03.2020.
- İrtegün S, Deveci E. Diyabetik sıçanların testis dokusunda VEGF ve Bcl-2 ekspresyon düzeylerinin immünohistokimya ve western blot yöntemleri ile incelenmesi. Dicle Tıp Dergisi 2016; 43(4) : 527-33.
- Kırpık MA, Altunoğlu MK, Yıldız Asker Y, Altunkalem S. Evelik Bitkisi (Rumex patientia L.) ve Karahindi-ba (Taraxacum officinale L.) kullanılarak doğal yolla baklagil yaprak biti (Aphis fabae Scop.)’nin hedef konukçusunun değiştirilmesi. KAUFBED 2019; 12(1): 1-8.
- Krause William J. Krause's Essential Human Hıstolo-gy for Medical Students. Third Edition. Boca Raton: Universal Publishers, 2005; pp. 224-36.
- La Vignera S, Calogero AE, Condorelli R, Lanzafame F, Giammusso B, Vicari E. Andrological characterization of the patient with diabetes mellitus. Minerva Endocrinol 2009; 34(1): 1-9.
- Lee MW, Kwon JE, Lee YJ, Jeong YJ, Kim I, Cho YM, Kim YM, Kang SC. Prunus mume leaf extract lowers blood glucose level in diabetic mice. Pharm Biol 2016; 54(10): 2135-40.
- Martinez M, Poirrier P, Chamy R, Prüfer D, Schulze-Gronover C, Jorquera L, Ruiz G. Taraxacum offici-nale and related species-An ethnopharmacological review and its potential as a commercial medicinal plant. J Ethnopharmacol 2015; 169(1): 244-62.
- Moyer JR, Boswall AL, Kawchuk LM, Entz T, Tovell B, Lee B. Characterization of dandelion (Taraxacum officinale Weber in FH Wigg.) biotype morphology, chemical composition and response to glyphosate. Can J Plant Sci 2009; 89(2): 369-78.
- Oroojan AA, Ahangarpour A, Paknejad B, Zareian P, Hami Z, Abtahi SR. Effects of myricitrin and solid lipid nanoparticle containing myricitrin on reproductive system disorders ınduced by diabetes in male mouse. World J Mens Health 2021; 39(1): 147-57.
- Öztürk F, Gül M, Ağkadir M, Yağmurca M. Deneysel diyabetin sıçan testislerinde meydana getirdiği his-tolojik değişiklikler. Turk Klinikleri J Med Sci 2002; 22(2): 173-8.
- Park CM, Park JY, Noh KH, Shin JH, Song YS. Ta-raxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells. J Ethnopharmacol 2011; 33(2): 834-42.
- Sadik NA, El-Seweidy MM, Shaker OG. The antiapoptotic effects of sulphurous mineral water and sodium hydrosulphide on diabetic rat testes. Cell Physiol Biochem 2011; 28(5): 887-98.
- Sarı A, Özsoy N, Karahüseyin S. Taraxacum farino-sum Hausskn. & Bornm. bitkisinin antioksidan ak-tivite yönünden incelenmesi. SABİAD 2020; 3(1): 13-9.
- Sarı AO, Bilgin O, Bilgiç A, Nedret T, Güvensen A, Şenol SG. Ege ve Güney Marmara bölgelerinde halk ilacı olarak kullanılan bitkiler. Anadolu ETAE dergisi 2010; 20(2): 1-21.
- Schütz K, Carle R, Schieber A. Taraxacum-a review on its phytochemical and pharmacological profile. J Ethnopharmacol 2006; 107(3): 313-23.
- Sebai H, Selmi S, Rtibi K, Gharbi N, Sakly M. Protective effect of Lavandula stoechas and Rosmarinus officinalis essential oils against reproductive dam-age and oxidative stress in alloxan-induced diabetic rats. J Med Food 2015; 18(2): 241-9.
- Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J of Bot 2012; 217037.
- Sharma R, Satyanarayana P, Anand P, Kumari GA. Adiponectin level association with MDA in the patients with type 2 diabetes mellitus. Biomed Pharmacol J 2020; 13: 1-9.
- Sies H. Oxidative stress: Oxidants and antioxidants. Exp Physiol 1997; 82: 291-5.
- Sua H, Tianb CJ, Wangb Y, Shib J, Chenb X, Zhena Z, Baia Y, Denga L, Fenga C, Mab Z, Liua J. Ginsenoside Rb1 reduces oxidative/carbonyl stress damage and ameliorates inflammation in the lung of streptozotocin-induced diabetic rats. Pharm Biol 2022; 60(1): 2229-36.
- Tian CJ, Zhen Z. 2019. Reactive carbonyl species: diabetic complication in the heart and lungs. Trends Endocrinol Metab 2019; 30(8): 546-56.
- Yigitturk G, Acara AC, Erbas O, Oltulu F, Yavasoglu NUK, Uysal A, Yavasoglu A. The antioxidant role of agomelatine and gallic acid on oxidative stress in STZ induced type I diabetic rat testes. Biomed Pharmacother 2017; 87: 240-6.
Karahindiba (Taraxacum officinale) Ekstraktı Uygulanan Diyabetik Ratların Testis Dokusunda Histolojik ve İmmunohistokimyasal İncelemeler
Abstract
Bu çalışma deneysel diyabet oluşturulan ratlarda Karahindiba (Taraxacum officinale) ekstraktı uygulamasının testis dokusunda meydana getirdiği değişimleri ve enzimatik antioksidanlardan SOD-2 salınımı üzerindeki etkilerini immuno-histokimyasal yöntemlerle ile değerlendirmek amacı ile yapıldı. Diyabet grubu testis kesitlerinde seminifer tübül yapısında yer yer dejeneratif değişiklikler izlendi. Bazal membran ile tübül epiteli arasında ayrışmalar, tübül lümenine doğru spermatogenik hücrelerin yapısında ve yerleşiminde bozulmalar, bazal membran ve tunika albugineada kalınlaşmalar, sertoli hücrelerinde şekil değişikliği ve leyding hücre çekirdeğinde küçülmeler olduğu belirlendi. Diyabet grubunda spermatogonyumlar ve primer spermatositlerde immun pozitiflik gözlenmezken, spermatogenik seriye ait diğer hücrelerde zayıf, diyabet+karahindiba gurubunda orta derecede SOD-2 immunoreaktivitesi gözlendi. Sonuç olarak kronik metabolik bir hastalık olan diyabet hastalığında testis dokusunda histopatolojik değişimlerin meydana geldiği ve SOD-2 immunoreaktivitesinin azaldığı belirlendi. Karahindiba uygulamasının azalmış olan SOD-2 immuno-reaktivitesi üzerinde olumlu etkileri olabileceği kanısına varıldı.
Keywords
References
- Al-Salmi AF, Hamza RZ. Efficacy of vanadyl sulfate and selenium tetrachloride as anti-diabetic agents against hyperglycemia and oxidative stress induced by diabetes mellitus in male rats. Curr Is-sues Mol Biol 2022; 44 (1): 94-104.
- Bener A, Al-Ansari AA, Zirie M, Al-Hamaq AO. Is male fertility associated with type 2 diabetes melli-tus? Int Urol Nephrol 2009; 41(4): 777-84.
- Choi UK, Lee OH, Yim JH, Cho CW, Rhee YK, Lim SI, Kim YC. Hypolipidemic and antioxidant effects of dandelion (taraxacum officinale) root and leaf on cholesterol-fed rabbits. Int J Mol Sci 2010; 11(1): 67-78.
- Dongmei C. Atlas of Histology with Functional and Clinical Correlations. First Edition. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wil-kins, 2011; p.344-68.
- Donmez YB, Kizilay G, Topcu-Tarladacalisir Y. MAPK immunoreactivity in streptozotocin-induced diabetic rat testis. Acta Cir Bras 2014; 29(10): 644-50.
- Ersoy O, Kizilay G. Effects of fucoidan on diabetic rat testicular tissue. Biotech Histochem 2018; 93(4): 277-85.
- Fridovich I. Superoxide dismutases. Annu Rev Bio-chem 1975; 44: 147-59.
- Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cil-aker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res 2008; 40(4): 354-60.
- International Diabetes Federation, Diabetes atlas 9th edition. https://www.diabetesatlas.org/en/sections/worldwide-toll-of-diabetes.html. 2019. Access: 24.03.2020.
- İrtegün S, Deveci E. Diyabetik sıçanların testis dokusunda VEGF ve Bcl-2 ekspresyon düzeylerinin immünohistokimya ve western blot yöntemleri ile incelenmesi. Dicle Tıp Dergisi 2016; 43(4) : 527-33.
- Kırpık MA, Altunoğlu MK, Yıldız Asker Y, Altunkalem S. Evelik Bitkisi (Rumex patientia L.) ve Karahindi-ba (Taraxacum officinale L.) kullanılarak doğal yolla baklagil yaprak biti (Aphis fabae Scop.)’nin hedef konukçusunun değiştirilmesi. KAUFBED 2019; 12(1): 1-8.
- Krause William J. Krause's Essential Human Hıstolo-gy for Medical Students. Third Edition. Boca Raton: Universal Publishers, 2005; pp. 224-36.
- La Vignera S, Calogero AE, Condorelli R, Lanzafame F, Giammusso B, Vicari E. Andrological characterization of the patient with diabetes mellitus. Minerva Endocrinol 2009; 34(1): 1-9.
- Lee MW, Kwon JE, Lee YJ, Jeong YJ, Kim I, Cho YM, Kim YM, Kang SC. Prunus mume leaf extract lowers blood glucose level in diabetic mice. Pharm Biol 2016; 54(10): 2135-40.
- Martinez M, Poirrier P, Chamy R, Prüfer D, Schulze-Gronover C, Jorquera L, Ruiz G. Taraxacum offici-nale and related species-An ethnopharmacological review and its potential as a commercial medicinal plant. J Ethnopharmacol 2015; 169(1): 244-62.
- Moyer JR, Boswall AL, Kawchuk LM, Entz T, Tovell B, Lee B. Characterization of dandelion (Taraxacum officinale Weber in FH Wigg.) biotype morphology, chemical composition and response to glyphosate. Can J Plant Sci 2009; 89(2): 369-78.
- Oroojan AA, Ahangarpour A, Paknejad B, Zareian P, Hami Z, Abtahi SR. Effects of myricitrin and solid lipid nanoparticle containing myricitrin on reproductive system disorders ınduced by diabetes in male mouse. World J Mens Health 2021; 39(1): 147-57.
- Öztürk F, Gül M, Ağkadir M, Yağmurca M. Deneysel diyabetin sıçan testislerinde meydana getirdiği his-tolojik değişiklikler. Turk Klinikleri J Med Sci 2002; 22(2): 173-8.
- Park CM, Park JY, Noh KH, Shin JH, Song YS. Ta-raxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells. J Ethnopharmacol 2011; 33(2): 834-42.
- Sadik NA, El-Seweidy MM, Shaker OG. The antiapoptotic effects of sulphurous mineral water and sodium hydrosulphide on diabetic rat testes. Cell Physiol Biochem 2011; 28(5): 887-98.
- Sarı A, Özsoy N, Karahüseyin S. Taraxacum farino-sum Hausskn. & Bornm. bitkisinin antioksidan ak-tivite yönünden incelenmesi. SABİAD 2020; 3(1): 13-9.
- Sarı AO, Bilgin O, Bilgiç A, Nedret T, Güvensen A, Şenol SG. Ege ve Güney Marmara bölgelerinde halk ilacı olarak kullanılan bitkiler. Anadolu ETAE dergisi 2010; 20(2): 1-21.
- Schütz K, Carle R, Schieber A. Taraxacum-a review on its phytochemical and pharmacological profile. J Ethnopharmacol 2006; 107(3): 313-23.
- Sebai H, Selmi S, Rtibi K, Gharbi N, Sakly M. Protective effect of Lavandula stoechas and Rosmarinus officinalis essential oils against reproductive dam-age and oxidative stress in alloxan-induced diabetic rats. J Med Food 2015; 18(2): 241-9.
- Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J of Bot 2012; 217037.
- Sharma R, Satyanarayana P, Anand P, Kumari GA. Adiponectin level association with MDA in the patients with type 2 diabetes mellitus. Biomed Pharmacol J 2020; 13: 1-9.
- Sies H. Oxidative stress: Oxidants and antioxidants. Exp Physiol 1997; 82: 291-5.
- Sua H, Tianb CJ, Wangb Y, Shib J, Chenb X, Zhena Z, Baia Y, Denga L, Fenga C, Mab Z, Liua J. Ginsenoside Rb1 reduces oxidative/carbonyl stress damage and ameliorates inflammation in the lung of streptozotocin-induced diabetic rats. Pharm Biol 2022; 60(1): 2229-36.
- Tian CJ, Zhen Z. 2019. Reactive carbonyl species: diabetic complication in the heart and lungs. Trends Endocrinol Metab 2019; 30(8): 546-56.
- Yigitturk G, Acara AC, Erbas O, Oltulu F, Yavasoglu NUK, Uysal A, Yavasoglu A. The antioxidant role of agomelatine and gallic acid on oxidative stress in STZ induced type I diabetic rat testes. Biomed Pharmacother 2017; 87: 240-6.
There are 30 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Histology and Embryology |
| Journal Section | Articles |
| Authors | |
| Publication Date | April 1, 2024 |
| Submission Date | November 20, 2023 |
| Acceptance Date | December 28, 2023 |
| Published in Issue | Year 2024 Volume: 21 Issue: 1 |
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https://dergipark.org.tr/tr/download/journal-file/20610
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