Abstract
Bovine tuberculosis, as a significant threat to both animal and human health, is a common global zoonotic disease. The emergence of molecular epidemiology has made it possible to gain a better understanding of the dynamics of disease transmission, and consequently, to come up with more effective control methods. The present study seeks to identify Mycobacterium bovis isolates in our region at a genotype level. To this end, the molecular epidemiological characteristics of Mycobacterium bovis strains isolated using classical methods and identified using molecular methods from the tissues and organs of cattle with suspected tuberculosis, obtained from slaughterhouses in the Konya province, or from those sent to Konya Veterinary Control Institute, were determined through genotyping. In the analysis of a total of 70 Mycobacterium bovis isolates, carried out using the MIRU-VNTR method, it was found that the repeat numbers for MIRU2, MIRU4, MIRU20, MIRU23, MIRU24, MIRU27 and MIRU39 loci did not vary between strains, while the repeat numbers for MIRU10, MIRU16, MIRU26, MIRU31, and MIRU40 loci varied between strains and had a high discriminatory power (0.25 ≤ h). It was further observed that 29 subgroups between 1-14 isolates formed. The movement of animals in our region, which occurs for several reasons, is considered to cause Mycobacterium bovis strains to vary between herds, and the fact that the cattle from which the isolates were sourced for our study came from different herds was considered to cause a variation in the discriminatory power in the MIRU loci.
Supporting Institution
Bu çalışma Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından BAP-09202051 proje numarası ile desteklenmiştir, yazarların herhangi bir çıkara dayalı ilişkisi yoktur.
Project Number
BAP-09202051
Thanks
The authors gratefully thank the Scientific Research Projects Coordination Unit of Selcuk University for their financial contributions to the study (BAP-09202051).
References
- Allix C, Walravens K, Saegerman C, Godfroid J, Supply P, Fauville-Dufaux M (2006) Evaluation of the Epidemiological Relevance of Variable-Number Tandem-Repeat Genotyping of Mycobacterium bovis and comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping. Journal of Clinical Microbiology. 44 (6):1951–1962. doi: 10.1128/JCM.01775-0.
- Bolado-Martínez E, Benavides-Dávila I, Candia-Plata MC, Navarro-Navarro M, Avilés-Acosta M, Álvarez-Hernández G. (2015) Proposal of a screening MIRU-VNTR Panel for the preliminary genotyping of Mycobacterium bovis in Mexico. BioMed Research International. 1-7. doi: 10.1155/2015/416479
- Boniotti MB, Goria M, Loda D, Garrone A, Benedetto A, Mondo A, Tisato E, Zanoni M, Zoppi S, Dondo A, Tagliabue S, Bonora S, Zanardi G, Pacciarini ML. (2009) Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variable-number tandem repeats for geographically optimized genotyping. Journal of Clinical Microbiology. 47(3), 636-644. doi: 10.1128/JCM.01192-08
- Besirovic H, Amer A, Spicic S, Cvetnic Z, Prasovic S, Velic L. (2012) Bovine tuberculosis in Bosnia and Herzegovina caused by Mycobacterium caprae. Vet. Arhiv. 82(4), 341-349
- Carvalho RCT, Vasconcellos SEG, Issa MA, Filho PMS, Mota PMPC, de Araújo FR, Carvalho ACS, Gomes HM, Suffys PN, Figueiredo EES, Paschoalin VMF. (2016) Molecular typing of Mycobacterium bovis from cattle reared in Midwest Brazil. Plos One. 1-16. doi:10.1371/journal.pone.0162459
- Duarte EL, Domingos E, Amado A, Cunha MV, Botelho A. (2010) MIRU-VNTR typing adds discriminatory value to groups of Mycobacterium Bovis and Mycobacterium caprae strains defined by spoligotyping. Veterinary Microbiology. 143, 299-306. doi: 10.1016/j.vetmic.2009.11.027
- Egbe NF, Muwonge A, Ndip L, Kelly RF, Sander M, Tanya V, Ngu Ngwa V, Handel IG, Novak A, Ngandalo R, Mazeri S, Morgan KL, Asuquo A, Bronsvoort BMC. (2017) Molecular epidemiology of Mycobacterium bovis in Cameroon. Scientific Reports. 7 (4652) doi: 10.1038/s41598-017-04230-6
- El Sayed MS. (2019) A first insight into the application of high discriminatory MIRU-VNTR typing using QIAxcel technology for genotyping Mycobacterium bovis isolated from the Delta area in Egypt. Infection. Genetics and Evolution. 71, 211-214. doi: 10.1016/j.meegid.2019.04.004
- Esen N. (2003) Tanı ve ilaç duyarlılık testlerinde moleküler yöntemlerin değeri. In: 21. Yüzyılda Tüberküloz Sempozyumu ve II. Tüberküloz Tanı Yöntemleri Kursu. Samsun, Türkiye; 402-408.
- Figueiredo EES, Ramos DF, Medeiros L, Silvestre FG, Lilenbaum W, Silva JT, Paschoalin VMF, Dellagostin OA. (2012) Multiple strains of Mycobacterium bovis revealed by molecular typing in a herd of cattle. The Veterinary Journal. 193, 296-298 doi: 10.1016/j.tvjl.2011.11.006
- Frothingham R, Meeker-O'Connel WA. (1998) Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats. Microbiology. 144(1), 1189-1196. doi :10.1099/00221287-144-5-1189
- Hilty M, Diguimbaye C, Schelling E, Baggi F, Tanner M, Zinsstag J. (2005) Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains. Veterinary Microbiology. 109, 217-222. doi:10.1016/j.vetmic.2005.05.017
- Jeon BY, Je S, Park J, Kim Y, Lee EG, Lee H, Seo S, Cho SN. (2008) Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea. Journal Veterinary Science. 9(2), 145-153. doi: 10.4142/jvs.2008.9.2.145
- Köksalan OK (2010). Günümüzde tüberküloz tanısı/güçlükleri. Ankem Dergisi. 24(2), 61-63
- Laniado-Laborín R, Muñiz-Salazar R, García-Ortiz RA, Vargas-Ojeda AC, Villa-Rosas C, Oceguera-Palao L. (2014) Molecular characterization of Mycobacterium bovis isolates from patients with tuberculosis in Baja California, Mexico. Infection, Genetics and Evolution. 27, 1-5. doi : 10.1016/j.meegid.2014.06.020
- Matos F, Amado A, Botelho A. (2010) Molecular typing of Mycobacterium bovis isolated in the first outbreak of bovine tuberculosis in the Azores Islands: a case report. Veterinarni Medicina. 55(3), 133-136. doi: 10.17221/137/2009-VETMED
- Oral Zeytinli Ü, Köksal F. (2012) Genotyping of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in Cukurova region, Turkey by spoligotyping and MIRU-VNTR methods. Mikrobiyol Bul. 46(2), 202-210.
- Parreiras PM, Andrade GI, Nascimento TF, Oelemann MC, Gomes HM, Alencar AP, Assis RA, Mota PMPC, Pareira MAS, Lobato FCF, Lage AP, Suffys PN. (2012) Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Memórias do Instituto Oswaldo Cruz. 107(1), 64-73. doi: 10.1590/S0074-02762012000100009
- Peterson R. (2009). Molecular Epidemiology of Tuberculosis. Karolinska Institutet. Phd. Stockholm. Sweden. Roring S, Scotta AN, Hewinsonb RG, Neillc SD, Skuce RA. (2004) Evaluation of variable number tandem repeat (VNTR) loci in molecular typing of Mycobacterium bovis isolates from Ireland. Veterinary Microbiology. 101, 65–73. doi:10.1016/j.vetmic.2004.02.013
- Skuce RA, McCorry TP, McCarroll JF, Roring SMM, Scott AN, Brittain D, Hughes SL, Hewinson RG, Neill SD. (2002). Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets. Microbiology. 148, 519-528. doi: 10.1099/00221287-148-2-519
- Supply P, Allix C, Lesjean S, Cardoso-Oelemann M, Rüsch-Gerdes S, Willery E, Savine E, Haas P, van Deutekom H, Roring S, Bifani P, Kurepina N, Kreiswirth B, Sola C, Rastogi N, Vatin V, Gutierrez MC, Fauville M, Niemann S, Skuce R, Kremer K, Locht C, van Soolingen D. (2006) Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. Journal of Clinical Microbiology. 44(12), 4498-4510.
- Vargas AN, Suazo FM, Alarcónb GJC, Venegas YR, Solorio RG, Hernández ER, Martìnez OP. (2016) Genetic diversity based on MIRU-VNTR profile of isolates of Mycobacterium bovis from Mexican cattle. Prevente Veterinary Medicine. 2016; 131, 75-78. doi: 10.1016/j.prevetmed.2016.07.007
- Wedlock DN, Skinner MA, Lisle GW, Buddle BM. (2002) Control of Mycobacterium bovis infections and the risk to human populations. Microbes and Infection. 4, 471-480. doi: 10.1016/S1286-4579(02)01562-9
- Yang L, Wang C, Wang H, Meng Q, Wang Q. (2015) Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China. Veterinary Research 11(93), 1-7. doi: 10.1186/s12917-015-0402-0
Abstract
Sadece hayvan sağlığı değil aynı zamanda halk sağlığı açısından da önem arz eden sığır tüberkülozu, dünya genelinde yaygın olan zoonotik bir hastalıktır. Moleküler epidemiyolojinin ortaya çıkışıyla, hastalığın yayılma dinamiğinin anlaşılması ve daha etkili mücadele yöntemlerinin geliştirilebilmesinin temelleri atılmıştır. Bu çalışmada, bölgemizdeki Mycobacterium bovis izolatlarının genotip düzeyinde tanımlanması amaçlandı. Çalışmaya, Konya’daki kesimhanelerden alınan veya Konya Veteriner Kontrol Enstitüsü’ne teşhis için gönderilen tüberküloz şüpheli sığır doku ve organlarından klasik metotlarla izole edilen ve moleküler metotlar ile tanımlanan M. bovis suşlarının genotiplendirilmesi sonucu moleküler epidemiyolojik özellikleri belirlendi. Toplam 70 M. bovis izolatının, MIRU-VNTR yöntemi ile yapılan analizi sonucu; MIRU2, MIRU4, MIRU20, MIRU23, MIRU24, MIRU27 ve MIRU39 lokuslarındaki tekrar sayılarının suşlar arasında farklılık göstermediği, MIRU10, MIRU16, MIRU26, MIRU31 ve MIRU40 lokuslarındaki tekrar sayılarının ise suşlar arasında farklılık gösterdiği ve yüksek ayrım gücüne sahip olduğu (0.25≤h) ortaya kondu. Ayrıca izolat sayısı 1-14 arasında değişen 29 altgrup oluştuğu görüldü. Bölgemizde farklı sebepler ile yaşanan hayvan hareketliliğinin, M. bovis suşlarının sürüler arasında farklılık göstermesine, çalışmamızda ki izolatların kaynağı olan sığırların da farklı sürülere ait olmasının MIRU lokuslarında ayrım gücü çeşitliliğinin ortaya çıkmasına neden olduğu kanaatine varılmıştır.
Project Number
BAP-09202051
References
- Allix C, Walravens K, Saegerman C, Godfroid J, Supply P, Fauville-Dufaux M (2006) Evaluation of the Epidemiological Relevance of Variable-Number Tandem-Repeat Genotyping of Mycobacterium bovis and comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping. Journal of Clinical Microbiology. 44 (6):1951–1962. doi: 10.1128/JCM.01775-0.
- Bolado-Martínez E, Benavides-Dávila I, Candia-Plata MC, Navarro-Navarro M, Avilés-Acosta M, Álvarez-Hernández G. (2015) Proposal of a screening MIRU-VNTR Panel for the preliminary genotyping of Mycobacterium bovis in Mexico. BioMed Research International. 1-7. doi: 10.1155/2015/416479
- Boniotti MB, Goria M, Loda D, Garrone A, Benedetto A, Mondo A, Tisato E, Zanoni M, Zoppi S, Dondo A, Tagliabue S, Bonora S, Zanardi G, Pacciarini ML. (2009) Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variable-number tandem repeats for geographically optimized genotyping. Journal of Clinical Microbiology. 47(3), 636-644. doi: 10.1128/JCM.01192-08
- Besirovic H, Amer A, Spicic S, Cvetnic Z, Prasovic S, Velic L. (2012) Bovine tuberculosis in Bosnia and Herzegovina caused by Mycobacterium caprae. Vet. Arhiv. 82(4), 341-349
- Carvalho RCT, Vasconcellos SEG, Issa MA, Filho PMS, Mota PMPC, de Araújo FR, Carvalho ACS, Gomes HM, Suffys PN, Figueiredo EES, Paschoalin VMF. (2016) Molecular typing of Mycobacterium bovis from cattle reared in Midwest Brazil. Plos One. 1-16. doi:10.1371/journal.pone.0162459
- Duarte EL, Domingos E, Amado A, Cunha MV, Botelho A. (2010) MIRU-VNTR typing adds discriminatory value to groups of Mycobacterium Bovis and Mycobacterium caprae strains defined by spoligotyping. Veterinary Microbiology. 143, 299-306. doi: 10.1016/j.vetmic.2009.11.027
- Egbe NF, Muwonge A, Ndip L, Kelly RF, Sander M, Tanya V, Ngu Ngwa V, Handel IG, Novak A, Ngandalo R, Mazeri S, Morgan KL, Asuquo A, Bronsvoort BMC. (2017) Molecular epidemiology of Mycobacterium bovis in Cameroon. Scientific Reports. 7 (4652) doi: 10.1038/s41598-017-04230-6
- El Sayed MS. (2019) A first insight into the application of high discriminatory MIRU-VNTR typing using QIAxcel technology for genotyping Mycobacterium bovis isolated from the Delta area in Egypt. Infection. Genetics and Evolution. 71, 211-214. doi: 10.1016/j.meegid.2019.04.004
- Esen N. (2003) Tanı ve ilaç duyarlılık testlerinde moleküler yöntemlerin değeri. In: 21. Yüzyılda Tüberküloz Sempozyumu ve II. Tüberküloz Tanı Yöntemleri Kursu. Samsun, Türkiye; 402-408.
- Figueiredo EES, Ramos DF, Medeiros L, Silvestre FG, Lilenbaum W, Silva JT, Paschoalin VMF, Dellagostin OA. (2012) Multiple strains of Mycobacterium bovis revealed by molecular typing in a herd of cattle. The Veterinary Journal. 193, 296-298 doi: 10.1016/j.tvjl.2011.11.006
- Frothingham R, Meeker-O'Connel WA. (1998) Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats. Microbiology. 144(1), 1189-1196. doi :10.1099/00221287-144-5-1189
- Hilty M, Diguimbaye C, Schelling E, Baggi F, Tanner M, Zinsstag J. (2005) Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains. Veterinary Microbiology. 109, 217-222. doi:10.1016/j.vetmic.2005.05.017
- Jeon BY, Je S, Park J, Kim Y, Lee EG, Lee H, Seo S, Cho SN. (2008) Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea. Journal Veterinary Science. 9(2), 145-153. doi: 10.4142/jvs.2008.9.2.145
- Köksalan OK (2010). Günümüzde tüberküloz tanısı/güçlükleri. Ankem Dergisi. 24(2), 61-63
- Laniado-Laborín R, Muñiz-Salazar R, García-Ortiz RA, Vargas-Ojeda AC, Villa-Rosas C, Oceguera-Palao L. (2014) Molecular characterization of Mycobacterium bovis isolates from patients with tuberculosis in Baja California, Mexico. Infection, Genetics and Evolution. 27, 1-5. doi : 10.1016/j.meegid.2014.06.020
- Matos F, Amado A, Botelho A. (2010) Molecular typing of Mycobacterium bovis isolated in the first outbreak of bovine tuberculosis in the Azores Islands: a case report. Veterinarni Medicina. 55(3), 133-136. doi: 10.17221/137/2009-VETMED
- Oral Zeytinli Ü, Köksal F. (2012) Genotyping of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in Cukurova region, Turkey by spoligotyping and MIRU-VNTR methods. Mikrobiyol Bul. 46(2), 202-210.
- Parreiras PM, Andrade GI, Nascimento TF, Oelemann MC, Gomes HM, Alencar AP, Assis RA, Mota PMPC, Pareira MAS, Lobato FCF, Lage AP, Suffys PN. (2012) Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Memórias do Instituto Oswaldo Cruz. 107(1), 64-73. doi: 10.1590/S0074-02762012000100009
- Peterson R. (2009). Molecular Epidemiology of Tuberculosis. Karolinska Institutet. Phd. Stockholm. Sweden. Roring S, Scotta AN, Hewinsonb RG, Neillc SD, Skuce RA. (2004) Evaluation of variable number tandem repeat (VNTR) loci in molecular typing of Mycobacterium bovis isolates from Ireland. Veterinary Microbiology. 101, 65–73. doi:10.1016/j.vetmic.2004.02.013
- Skuce RA, McCorry TP, McCarroll JF, Roring SMM, Scott AN, Brittain D, Hughes SL, Hewinson RG, Neill SD. (2002). Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets. Microbiology. 148, 519-528. doi: 10.1099/00221287-148-2-519
- Supply P, Allix C, Lesjean S, Cardoso-Oelemann M, Rüsch-Gerdes S, Willery E, Savine E, Haas P, van Deutekom H, Roring S, Bifani P, Kurepina N, Kreiswirth B, Sola C, Rastogi N, Vatin V, Gutierrez MC, Fauville M, Niemann S, Skuce R, Kremer K, Locht C, van Soolingen D. (2006) Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. Journal of Clinical Microbiology. 44(12), 4498-4510.
- Vargas AN, Suazo FM, Alarcónb GJC, Venegas YR, Solorio RG, Hernández ER, Martìnez OP. (2016) Genetic diversity based on MIRU-VNTR profile of isolates of Mycobacterium bovis from Mexican cattle. Prevente Veterinary Medicine. 2016; 131, 75-78. doi: 10.1016/j.prevetmed.2016.07.007
- Wedlock DN, Skinner MA, Lisle GW, Buddle BM. (2002) Control of Mycobacterium bovis infections and the risk to human populations. Microbes and Infection. 4, 471-480. doi: 10.1016/S1286-4579(02)01562-9
- Yang L, Wang C, Wang H, Meng Q, Wang Q. (2015) Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China. Veterinary Research 11(93), 1-7. doi: 10.1186/s12917-015-0402-0
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Original Article |
| Authors | |
| Project Number | BAP-09202051 |
| Publication Date | December 31, 2020 |
| Submission Date | November 3, 2020 |
| Published in Issue | Year 2020 Volume: 31 Issue: 2 |
