PATHOLOGICAL AND BIOCHEMICAL INVESTIGATION OF THE EFFECTS OF SILYBUM MARIANUM AGAINST METHOMYL DAMAGE IN BROILER KIDNEY
Abstract
The aim of this study was to investigate the protective/preventive or ameliorative effects of Silybum marianum seed powder against kidney damage caused by Methomyl toxicity in broilers. In the 28-day study, 4 different groups of 32 animals each were formed as Control group (CONT), Methomyl group (MET), Silybum marianum seed powder group (SMT) and Methomyl+Silybum marianum seed powder group (MET+SMT). Methomyl 20 ppm and Silybum marianum seed powder 10 g/kg were added to the feed. During the experiment, 8 animals from each group were necropsied weekly and samples were taken for biochemical and histopathological examinations. Total protein, creatinine and uric acid parameters were measured from serum samples taken for biochemical examinations. In histopathological examinations, renal hyperemia, degeneration and necrosis of tubular epithelium, thickening of Bowman's capsule, hemorrhage and inflammatory cell infiltration in the intertubular area, and thickening of tubular basement membranes were evaluated. In the study, creatinine, uric acid and total protein values were found to be similar in the KONT and SMT groups, but statistically higher in the MET group compared to the KONT and SMT groups. In the MET+SMT group, the values of the related parameters were lower than in the MET group. In all methomyl-treated groups, damage to the kidney tissues became evident in the measurements on the 14th day and increased in severity on the 21st and 28th days in a time-dependent manner. Silybum marianum given for protective/healing purposes was observed to reduce the damage at biochemical and histopathological levels.
Project Number
yok.
References
- Ashour MB, Ahmed OM, Asran A, Ali MA, 2017. Assessment of the preventive effects of Salvia officinalis and Ruta graveolens ethanolic leaf extracts on chlorpyrifos-and Methomyl-induced renal toxicity and oxidative stress in albino rats. International journal of prevention and treatment, 6(2), 34-44. DOI: 10.5923/j.ijpt.20170602.03
- Aslanturk A, Kalender Y, 2021. Methomyl-induced nephrotoxicity and protective effect of curcumin in male rats. Toxicology research, 10, 1003-1012. doi.org/10.1093/toxres/tfab080
- Banerjee BD, Seth V, Ahmed RS, 2001. Pesticide-induced oxidative stress: perspective and trends. Reviews on environmental health, 16(1), 1-40. doi.org/10.1515/REVEH.2001.16.1.1
- Boucaud-Maitre D, Rambourg MO, Sinno-Tellier S, Puskarczyk E, Pineau X, Kammerer M, Bloch J, Langrand J, 2019. Human exposure to banned pesticides reported to the French Poison Control Centers: 2012–2016.
- Environmental toxicology and pharmacology, 69, 51-56. doi.org/10.1016/j.etap.2019.03.017
- Cecen E, Dost T, Culhaci N, Karul A, Ergur B, Birincioglu M, 2011. Protective effects of silymarin against doxorubicin-induced toxicity. Asian Pacific journal of cancer prevention: APJCP, 12(10), 2697-2704.
- Çelik AS, Kan Y, 2013. Konya ekolojik şartlarında yetiştirilen meryemana dikeni bitkisinin (Silybum marianum) tohum verimi, Silymarin ve sabit yağ bileşenlerinin belirlenmesi. selcuk journal of agriculture and food sciences, 27(1), 24-31.
- Çubukçu B, Meriçli AH, Mat A, Sarıyar G, Sütlüpınar N, Meriçli F, 2002. İ.Ü. eczacılık fakültesi farmakognozi anabilim dalı, fitoterapi yardımcı ders kitabı.
- Ding TM, Tian SJ, Zhang ZX, Gu DZ, Chen Y, Shi YH, Sun ZP, 2001. Determination of active component in silymarin by RP-LC and LC/MS. Journal of pharmaceutical and biomedical analysis, 26(1), 155-61. doi.org/10.1016/S0731-7085(01)00364-8
- Djeffal A, Messarah M, Boumendjel A, Kadeche L, Feki AE, 2015. Protective effects of vitamin C and selenium supplementation on Methomyl-induced tissue oxidative stress in adult rats. Toxicology and industrial health, 31(1), 31-43. doi.org/10.1177/0748233712468020
- El-Demerdash F, Dewer Y, ElMazoudy RH, Attia AA, 2013. Kidney antioxidant status, biochemical para MET ers and histopathological changes induced by Methomyl in CD-1 mice. Experimental and Toxicologic Pathology, 65(6), 897-01. doi.org/10.1016/j.etp.2013.01.002 Erişim 1: https://www.tarimorman.gov.tr/Konular/Bitki-Sagligi-Hizmetleri/Bitki-Koruma-Urunleri-Ve-Makinalari/Bitki-Koruma-Urunleri Erişim tarihi:24.07.2023
- Gür FM, Aktaş İ, 2022. Silymarin protects kidneys from paclitaxel-induced nephrotoxicity. Turkish Journal of Agriculture-Food Science and Technology, 10(3), 452-58. doi.org/10.24925/turjaf.v10i3.452-458.5119 Kocaman N, Dabak Ö, 2015. Hepatoprotektif bir ajan: silymarin. Fırat tıp dergisi, 20(3), 128-32.
- Krishnakumar T, Visvanathan R, 2014. Acrylamide in food products: a review. Journal of Food Processing and Technology, 5(7). DOI: 10.4172/2157-7110.1000344
- Lin Z, Zhang W, Pang S, Huang Y, Mishra S, Bhatt P, Chen S, 2020. Current approaches to and future perspectives on Methomyl degradation in contaminated soil/water environments. Molecules, 25(3), 738. doi.org/10.3390/molecules25030738
- Luna LG (1968): Manual of histologic staining Methods of the armed forces institute of pathology, 3rd edn. McGraw- Hill, New York.
- Makrides C, Koukouvas M, Achillews G, Tsikkos S, Vounou E, Symeonides M, Christodoulides P, Ioannides M, 2005. Methomyl-induced severe acute pancreatitis: possible etiological association. JOP, 6(2), 166-71.
- Mansour SA, Mohamed RI, Ali AR, 2015. Which exposure period to selenium is more protective against hepato-renal toxicity of Methomyl in male rats. J Biochem Int, 2, 105-18.
- Meyerholz DK, Tintle NL, Beck AP, 2019. Common pitfalls in analysis of tissue scores. Veterinary pathology, 56(1), 39-42. doi.org/10.1177/0300985818794250
- Sakr S, Hassanien H, Bester MJ, Arbi S, Sobhy A, El Negris H, Steenkamp V, 2018. Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to Methomyl. Toxicology research, 7(3), 480-91. doi.org/10.1039/c7tx00309a
- Shalaby MA, El Zorba HY, Ziada RM, 2010. Reproductive toxicity of Methomyl insecticide in male rats and protective effect of folic acid. Food and chemical toxicology, 48(11), 3221-3226. doi.org/10.1016/j.fct.2010.08.027
- Subapriya S, Vairamuthu S, Manohar BM, Balachandran C, 2007. Pathomorphological changes in thiram toxicosis in broiler chicken. International Journal of Poultry Science, 6(4), 251-54.
- Şentürk H, Kolankaya D, Şahin Y, 2010. Renal İskemi-Reperfüzyonu Sırasında Sıçan Böbreğinde Oluşan Oksidatif Stres Hasarına Silimarin Etkisi . Cankaya University Journal of Science and Engineering,7 (1).
Silybum Marianum'un Broiler Böbreğinde Methomyl Hasarına Karşı Etkilerinin Patolojik ve Biyokimyasal Olarak Araştırılması
Abstract
Bu çalışmada broilerlerde Methomyl toksikasyonuna bağlı oluşan böbrek hasarına karşı Silybum marianum tohumu tozunun koruyucu/önleyici ya da iyileştirici etkilerinin araştırılması amaçlanmıştır. 28 gün olarak planlanlanan çalışmada Konrol grubu (KONT), Methomyl grubu (MET), Silybum marianum tohumu tozu grubu (SMT) ve Methomyl+Silybum marianum tohumu tozu grubu (MET+SMT) olmak üzere her birisinde 32 hayvan bulunan 4 farklı grup oluşturuldu. Methomyl 20 ppm, Silybum marianum tohumu tozu ise 10 gr/kg olarak yemlere ilave edildi. Deneme süresince haftalık olarak her bir gruptan 8 hayvanın nekropsileri yapılarak biyokimyasal ve histopatolojik incelemeler için örnekler alındı. Biyokimyasal incelemeler için alınan serum örneklerinden total protein, kreatinin ve ürik asit parametrelerin ölçümleri yapıldı. Histopatolojik incelemelerde ise böbreklerde hiperemi, tubulus epitellerinde dejenerasyon ve nekroz, Bowman kapsülünde kalınlaşma, intertubuler alanda kanama ve yangısal hücre infiltrasyonu, tubulus bazal membranlarında kalınlaşma bulguları değerlendirildi. Çalışmada her hafta kan serumlarında yapılan analizlerde kreatinin, ürik asit ve total protein değerleri KONT ve SMT gruplarında benzer bulunurken, MET gruplarında, KONT ve SMT gruplarına kıyasla istatistiksel olarak yüksek saptanmıştır. MET+SMT grubunda ise ilgili parametrelerin değerleri MET grubuna göre düşük olduğu görülmüştür. Methomyl verilen tüm gruplarda, böbrek dokularında 14’üncü gün ölçümlerde belirginleşen, 21 ve 28’inci günlerde zamana bağlı olarak şiddeti artan hasar meydana gelmiştir. Koruyucu/iyileştirici amaçla verilen Silybum marianum’un ise biyokimyasal ve histopatolojik düzeyde hasarları azalttığı gözlemlenmiştir.
Ethical Statement
Bu makale 1. Yazarın “Yemlerine Methomyl Ve Silybum Marianum İlave Edilmiş Broilerlerde Patolojik Ve Biyokimyasal İncelemeler” Isimli doktora tez çalışmasından üretilmiştir. Çalışmanın yapılması Veteriner Kontrol Merkez Araştırma Enstitüsü Müdürlüğü Yerel Etik Kurulu tarafından onaylanmıştır (05.05.2021 tarih ve 2021/07 numaralı karar).
Supporting Institution
yok.
Project Number
yok.
Thanks
Biyokimya verilerinin değerlendirilmesindeki katkılarından dolayı Selçuk Üniversitesi Veteriner Fakültesi Öğretim Üyesi Prof. Dr. Vahdettin ALTUNOK’a teşekkür ederiz.
References
- Ashour MB, Ahmed OM, Asran A, Ali MA, 2017. Assessment of the preventive effects of Salvia officinalis and Ruta graveolens ethanolic leaf extracts on chlorpyrifos-and Methomyl-induced renal toxicity and oxidative stress in albino rats. International journal of prevention and treatment, 6(2), 34-44. DOI: 10.5923/j.ijpt.20170602.03
- Aslanturk A, Kalender Y, 2021. Methomyl-induced nephrotoxicity and protective effect of curcumin in male rats. Toxicology research, 10, 1003-1012. doi.org/10.1093/toxres/tfab080
- Banerjee BD, Seth V, Ahmed RS, 2001. Pesticide-induced oxidative stress: perspective and trends. Reviews on environmental health, 16(1), 1-40. doi.org/10.1515/REVEH.2001.16.1.1
- Boucaud-Maitre D, Rambourg MO, Sinno-Tellier S, Puskarczyk E, Pineau X, Kammerer M, Bloch J, Langrand J, 2019. Human exposure to banned pesticides reported to the French Poison Control Centers: 2012–2016.
- Environmental toxicology and pharmacology, 69, 51-56. doi.org/10.1016/j.etap.2019.03.017
- Cecen E, Dost T, Culhaci N, Karul A, Ergur B, Birincioglu M, 2011. Protective effects of silymarin against doxorubicin-induced toxicity. Asian Pacific journal of cancer prevention: APJCP, 12(10), 2697-2704.
- Çelik AS, Kan Y, 2013. Konya ekolojik şartlarında yetiştirilen meryemana dikeni bitkisinin (Silybum marianum) tohum verimi, Silymarin ve sabit yağ bileşenlerinin belirlenmesi. selcuk journal of agriculture and food sciences, 27(1), 24-31.
- Çubukçu B, Meriçli AH, Mat A, Sarıyar G, Sütlüpınar N, Meriçli F, 2002. İ.Ü. eczacılık fakültesi farmakognozi anabilim dalı, fitoterapi yardımcı ders kitabı.
- Ding TM, Tian SJ, Zhang ZX, Gu DZ, Chen Y, Shi YH, Sun ZP, 2001. Determination of active component in silymarin by RP-LC and LC/MS. Journal of pharmaceutical and biomedical analysis, 26(1), 155-61. doi.org/10.1016/S0731-7085(01)00364-8
- Djeffal A, Messarah M, Boumendjel A, Kadeche L, Feki AE, 2015. Protective effects of vitamin C and selenium supplementation on Methomyl-induced tissue oxidative stress in adult rats. Toxicology and industrial health, 31(1), 31-43. doi.org/10.1177/0748233712468020
- El-Demerdash F, Dewer Y, ElMazoudy RH, Attia AA, 2013. Kidney antioxidant status, biochemical para MET ers and histopathological changes induced by Methomyl in CD-1 mice. Experimental and Toxicologic Pathology, 65(6), 897-01. doi.org/10.1016/j.etp.2013.01.002 Erişim 1: https://www.tarimorman.gov.tr/Konular/Bitki-Sagligi-Hizmetleri/Bitki-Koruma-Urunleri-Ve-Makinalari/Bitki-Koruma-Urunleri Erişim tarihi:24.07.2023
- Gür FM, Aktaş İ, 2022. Silymarin protects kidneys from paclitaxel-induced nephrotoxicity. Turkish Journal of Agriculture-Food Science and Technology, 10(3), 452-58. doi.org/10.24925/turjaf.v10i3.452-458.5119 Kocaman N, Dabak Ö, 2015. Hepatoprotektif bir ajan: silymarin. Fırat tıp dergisi, 20(3), 128-32.
- Krishnakumar T, Visvanathan R, 2014. Acrylamide in food products: a review. Journal of Food Processing and Technology, 5(7). DOI: 10.4172/2157-7110.1000344
- Lin Z, Zhang W, Pang S, Huang Y, Mishra S, Bhatt P, Chen S, 2020. Current approaches to and future perspectives on Methomyl degradation in contaminated soil/water environments. Molecules, 25(3), 738. doi.org/10.3390/molecules25030738
- Luna LG (1968): Manual of histologic staining Methods of the armed forces institute of pathology, 3rd edn. McGraw- Hill, New York.
- Makrides C, Koukouvas M, Achillews G, Tsikkos S, Vounou E, Symeonides M, Christodoulides P, Ioannides M, 2005. Methomyl-induced severe acute pancreatitis: possible etiological association. JOP, 6(2), 166-71.
- Mansour SA, Mohamed RI, Ali AR, 2015. Which exposure period to selenium is more protective against hepato-renal toxicity of Methomyl in male rats. J Biochem Int, 2, 105-18.
- Meyerholz DK, Tintle NL, Beck AP, 2019. Common pitfalls in analysis of tissue scores. Veterinary pathology, 56(1), 39-42. doi.org/10.1177/0300985818794250
- Sakr S, Hassanien H, Bester MJ, Arbi S, Sobhy A, El Negris H, Steenkamp V, 2018. Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to Methomyl. Toxicology research, 7(3), 480-91. doi.org/10.1039/c7tx00309a
- Shalaby MA, El Zorba HY, Ziada RM, 2010. Reproductive toxicity of Methomyl insecticide in male rats and protective effect of folic acid. Food and chemical toxicology, 48(11), 3221-3226. doi.org/10.1016/j.fct.2010.08.027
- Subapriya S, Vairamuthu S, Manohar BM, Balachandran C, 2007. Pathomorphological changes in thiram toxicosis in broiler chicken. International Journal of Poultry Science, 6(4), 251-54.
- Şentürk H, Kolankaya D, Şahin Y, 2010. Renal İskemi-Reperfüzyonu Sırasında Sıçan Böbreğinde Oluşan Oksidatif Stres Hasarına Silimarin Etkisi . Cankaya University Journal of Science and Engineering,7 (1).
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Pathology |
| Journal Section | Original Article |
| Authors | |
| Project Number | yok. |
| Publication Date | December 28, 2023 |
| Submission Date | October 6, 2023 |
| Published in Issue | Year 2023 Volume: 34 Issue: 2 |
