Research Article
Avrupa Yayın Balığı (Silurus glanis L.)'nın Sindirim Kanalındaki Bazı Nöropeptidlerin İmmunohistokimyasal Olarak İncelenmesi
Abstract
İmmunohistokimyasal teknikler kullanılarak Avrupa yayın
balığı Silurus glanis’in mide ve bağırsağında gastrin, glukagon ve
kolesistokinin (CCK-8) belirlendi. Bu nöropeptidler mide ve bağırsağın farklı
bölümlerinde değişen yoğunluk ve lokalizasyonda tespit edildi. Gastrin ve
glukagon immunoreaktiviteleri midenin gastrik mukozasında belirlendi. Bu
immureaktif hücreler genellikle midenin yüzey ve bez epitel hücreleri arasında
lokalizedir. Gastrin, glukagon ve CCK-8 immunoreaktiviteleri bağırsakta
bulundu. Bağırsak mukozasındaki immunoreaktif hücreler yüzey epitel
hücrelerinde belirlendi. Ekonomik öneme sahip olan Silurus glanis’in sindirim
kanalında bu immunoreaktivitelerin ilk defa belirlenmiş olması, bu balığın kas ve
mukosal salgı aktivitesinin morfolojik ve fonksiyonel yönden
değerlendirilmesine katkı sağlamaktadır.
References
- 1. Linhart O, Setch L, Svarc J, Rodina M, Audebert JP, Grecu J, Billard R (2002). The culture of the European catfish, Silurus glanis, in the Czech Republic and in France. Aquatic Living Resources, 15: 139–144. 2. Pan QS, Fang ZP, Huang FJ (2000a). Identification, localization and morphology of APUD cells in gastroenteropancreatic system of stomach-containing teleosts. World Journal of Gastroenterology, 6(6):842-847. 3. Groff KE, John HY (1997). An immunohistochemical study of the endocrine cells within the pancreas, intestine, and stomach of the gar (Lepisosteus osseus L.). General and Comparative Endocrinology, 106: 1–6. 4. Solcia E, Capella C, Buffa R (1987). Endocrine cells of the digestive system. In: Johnson LR, ed. Physiology of the gastrointestinal tract. New York. Raven Press. 111p. 5. Sherwood NM, Kruecke SL, Mcrory JE (2000). The origin and function of the pituitary adenylate cyclase-activating polypeptide (PACAP)/glucagon superfamily. Endocrine Reviews, 21: 619–670. 6. Wren AM, Small CJ, Abbott CR, Dhillo WS, Seal LJ, Cohen MA, Batterham RL, Taheri S,Stanley SA, Ghatei MA, Bloom SR (2001). Ghrelin causes hyperphagia and obesity in rats. Diabetes, 50: 2540–2547. 7. Peyon P, Lin XW, Himick BA, Peter RE (1998). Molecular cloning and expression of cDNA encoding brain preprocholecystokinin in goldfish. Peptides, 19:199–210. 8. Pan QS, Fang ZP, Zhao YX (2000b). Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes. World Journal of Gastroenterology, 6: 96-101. 9. Çınar K, Diler A (2002). Immunohistochemical localization of glucagon, sunstance-P, and vasoactive intestinal peptide in gastrointestinal tract mucosa of zander. Journal of Fish Biology, 60: 319–327. 10. Kurokawa T, Suzuki T, Hashimoto H (2003). Identification of gastrin and multiple cholecystokinin genes in teleost. Peptides, 24: 227–235. 11. Bosi G, Giancamillo AD, Arrighi S, Domeneghini C (2004a). An immunohistochemical study on the neuroendocrine system in the alimentary canal of the Brown trout, Salmo trutta, L., 1758.. General and Comparative Endocrinology, 138: 166–181. 12. Lee JH, Ku SK, Park KD, Lee HS (2004). Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch. Journal of Fish Biology, 65(1): 170–181. 13. Min HE, Wang K, Zhang Y (2009). Immunocytochemical Identification and Localization of Diffuse Neuroendocrine System (DNES) Cells in Gastrointestinal Tract of Channel Catfish (Ictalurus punctatus). Agricultural Sciences in China, 8(2): 238-243. 14. Kuru N, Çınar K, Şenol N, Demirbağ E, Diler D (2010). Endocrine Cells in the Gastrointestinal Tract of Garra rufa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16 (Suppl-B): 235–241. 15. Vigliano FA, Munoz L, Hernandez D, Cerutti P, Bermudez R, Quiroga MI (2011). An immunohistochemical study of the gut neuroendocrine system in juvenile pejerrey Odontesthes bonariensis (Valenciennes). Journal of Fish Biology, 78: 901–911. 16. Hernandez DR, Vigliano FA, Sanchez S, Bermudez R, Domitrovic HA, Quiroga MI (2012). Neuroendocrine system of the digestive tract in Rhamdia quelen juvenile: an immunohistochemical study. Tissue and Cell, 44: 220–226. 17. Pereira RT, Costa LS, Oliveira IRC, Araujo JC, Aerts M, Vigliano PV (2015). Rosa Relative distribution of gastrin-, CCK-8-, NPY- and CGRP-immunoreactive cells in the digestive tract of dorado (Salminus brasiliensis) Tissue and Cell, 47: 123–131. 18. Lin X, Wang P, Ou Y, Li1 J, Wen J (2017). An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos (Forsskal, 1775). Aquaculture Research, 48: 1439–1449. 19. Ku SK, Lee JH, Lee HS (2004). Immunohistochemical study on the endocrine cells in the gut of the stomachless teleost, Zacco platypus (cyprinidae). Anatomia Histologia Embryologia, 33: 212–219. 20. Bosi G, Shinn AP, Giari L, Simoni F, Dezfuli BS (2005). Changes in the neuromodulators of the diffuse endocrine system of the alimentary canal of farmed rainbow trout, Oncorhynchus mykiss (Walbaum), naturally infected with Eubothrium crassum (Cestoda). Journal of Fish Diseases, 28: 703–711. 21. Stenberger LA, (1986), Immunocytochemistry. 3rd ed. Wiley, New York. 22. Shu S, Ju G, Fan L (1988). The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system. Neuroscience Letters, 85: 169-171. 23. Bjenning C, Holmgren S, (1988). Neuropeptides in the fish gut. An immunohistochemical study of evolutionary patterns. Histochemistry, 88: 155–163. 24. Ping HC, Feng K, Zhang GR, Wei KJ, Zou GW, Wang WM (2014). Ontogeny expression of ghrelin, neuropeptide Y and cholecystokinin in blunt snout bream, Megalobrama amblycephala. Journal of Animal Physiology and Animal Nutrition 98: 338–346. 25. Hur SW, Lee CH, Lee SH, Kim BH, Kim HB, Baek HJ, Lee YD (2013). Characterization of cholecystokinin-producing cells and mucus-secreting goblet cells in the blacktip grouper, Epinephelus fasciatus. Tissue and Cell 45: 153–157. 26. Gençer TB, Bayrakdar A. Yaman M (2012). Immunohistochemical study of the endocrine cells in the stomach and pyloric caeca of the mountain trout, Salmo trutta macrostigma. Iranian Journal of Fisheries Sciences 11(4): 776-785. 27. Şenol N, Çınar K, Eren Ü (2009). Sudak (Stizostedion Lucioperca L., 1758) Balığında Mide ve Bağırsakta Bazı Peptidlerin Lokalizasyonu. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi (e-dergi), 4(2): 136-150. 28. Diler D, Çınar K, Zorlu S (2011). An Immunohistochemical Study on the Endocrine Cells in the Stomach and Intestine Regions of the Dicentrarchus labrax, L.,1758. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 25 (1): 01 – 06. 29. Jensen J (2001). Regulatory peptides and control of food intake in non-mammalian vertebrates. Comparative Biochemistry and Physiology, 128: 471–479.
Year 2018,
Volume: 30 Issue: 1, 15 - 21, 01.03.2018
Abstract
References
- 1. Linhart O, Setch L, Svarc J, Rodina M, Audebert JP, Grecu J, Billard R (2002). The culture of the European catfish, Silurus glanis, in the Czech Republic and in France. Aquatic Living Resources, 15: 139–144. 2. Pan QS, Fang ZP, Huang FJ (2000a). Identification, localization and morphology of APUD cells in gastroenteropancreatic system of stomach-containing teleosts. World Journal of Gastroenterology, 6(6):842-847. 3. Groff KE, John HY (1997). An immunohistochemical study of the endocrine cells within the pancreas, intestine, and stomach of the gar (Lepisosteus osseus L.). General and Comparative Endocrinology, 106: 1–6. 4. Solcia E, Capella C, Buffa R (1987). Endocrine cells of the digestive system. In: Johnson LR, ed. Physiology of the gastrointestinal tract. New York. Raven Press. 111p. 5. Sherwood NM, Kruecke SL, Mcrory JE (2000). The origin and function of the pituitary adenylate cyclase-activating polypeptide (PACAP)/glucagon superfamily. Endocrine Reviews, 21: 619–670. 6. Wren AM, Small CJ, Abbott CR, Dhillo WS, Seal LJ, Cohen MA, Batterham RL, Taheri S,Stanley SA, Ghatei MA, Bloom SR (2001). Ghrelin causes hyperphagia and obesity in rats. Diabetes, 50: 2540–2547. 7. Peyon P, Lin XW, Himick BA, Peter RE (1998). Molecular cloning and expression of cDNA encoding brain preprocholecystokinin in goldfish. Peptides, 19:199–210. 8. Pan QS, Fang ZP, Zhao YX (2000b). Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes. World Journal of Gastroenterology, 6: 96-101. 9. Çınar K, Diler A (2002). Immunohistochemical localization of glucagon, sunstance-P, and vasoactive intestinal peptide in gastrointestinal tract mucosa of zander. Journal of Fish Biology, 60: 319–327. 10. Kurokawa T, Suzuki T, Hashimoto H (2003). Identification of gastrin and multiple cholecystokinin genes in teleost. Peptides, 24: 227–235. 11. Bosi G, Giancamillo AD, Arrighi S, Domeneghini C (2004a). An immunohistochemical study on the neuroendocrine system in the alimentary canal of the Brown trout, Salmo trutta, L., 1758.. General and Comparative Endocrinology, 138: 166–181. 12. Lee JH, Ku SK, Park KD, Lee HS (2004). Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch. Journal of Fish Biology, 65(1): 170–181. 13. Min HE, Wang K, Zhang Y (2009). Immunocytochemical Identification and Localization of Diffuse Neuroendocrine System (DNES) Cells in Gastrointestinal Tract of Channel Catfish (Ictalurus punctatus). Agricultural Sciences in China, 8(2): 238-243. 14. Kuru N, Çınar K, Şenol N, Demirbağ E, Diler D (2010). Endocrine Cells in the Gastrointestinal Tract of Garra rufa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16 (Suppl-B): 235–241. 15. Vigliano FA, Munoz L, Hernandez D, Cerutti P, Bermudez R, Quiroga MI (2011). An immunohistochemical study of the gut neuroendocrine system in juvenile pejerrey Odontesthes bonariensis (Valenciennes). Journal of Fish Biology, 78: 901–911. 16. Hernandez DR, Vigliano FA, Sanchez S, Bermudez R, Domitrovic HA, Quiroga MI (2012). Neuroendocrine system of the digestive tract in Rhamdia quelen juvenile: an immunohistochemical study. Tissue and Cell, 44: 220–226. 17. Pereira RT, Costa LS, Oliveira IRC, Araujo JC, Aerts M, Vigliano PV (2015). Rosa Relative distribution of gastrin-, CCK-8-, NPY- and CGRP-immunoreactive cells in the digestive tract of dorado (Salminus brasiliensis) Tissue and Cell, 47: 123–131. 18. Lin X, Wang P, Ou Y, Li1 J, Wen J (2017). An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos (Forsskal, 1775). Aquaculture Research, 48: 1439–1449. 19. Ku SK, Lee JH, Lee HS (2004). Immunohistochemical study on the endocrine cells in the gut of the stomachless teleost, Zacco platypus (cyprinidae). Anatomia Histologia Embryologia, 33: 212–219. 20. Bosi G, Shinn AP, Giari L, Simoni F, Dezfuli BS (2005). Changes in the neuromodulators of the diffuse endocrine system of the alimentary canal of farmed rainbow trout, Oncorhynchus mykiss (Walbaum), naturally infected with Eubothrium crassum (Cestoda). Journal of Fish Diseases, 28: 703–711. 21. Stenberger LA, (1986), Immunocytochemistry. 3rd ed. Wiley, New York. 22. Shu S, Ju G, Fan L (1988). The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system. Neuroscience Letters, 85: 169-171. 23. Bjenning C, Holmgren S, (1988). Neuropeptides in the fish gut. An immunohistochemical study of evolutionary patterns. Histochemistry, 88: 155–163. 24. Ping HC, Feng K, Zhang GR, Wei KJ, Zou GW, Wang WM (2014). Ontogeny expression of ghrelin, neuropeptide Y and cholecystokinin in blunt snout bream, Megalobrama amblycephala. Journal of Animal Physiology and Animal Nutrition 98: 338–346. 25. Hur SW, Lee CH, Lee SH, Kim BH, Kim HB, Baek HJ, Lee YD (2013). Characterization of cholecystokinin-producing cells and mucus-secreting goblet cells in the blacktip grouper, Epinephelus fasciatus. Tissue and Cell 45: 153–157. 26. Gençer TB, Bayrakdar A. Yaman M (2012). Immunohistochemical study of the endocrine cells in the stomach and pyloric caeca of the mountain trout, Salmo trutta macrostigma. Iranian Journal of Fisheries Sciences 11(4): 776-785. 27. Şenol N, Çınar K, Eren Ü (2009). Sudak (Stizostedion Lucioperca L., 1758) Balığında Mide ve Bağırsakta Bazı Peptidlerin Lokalizasyonu. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi (e-dergi), 4(2): 136-150. 28. Diler D, Çınar K, Zorlu S (2011). An Immunohistochemical Study on the Endocrine Cells in the Stomach and Intestine Regions of the Dicentrarchus labrax, L.,1758. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 25 (1): 01 – 06. 29. Jensen J (2001). Regulatory peptides and control of food intake in non-mammalian vertebrates. Comparative Biochemistry and Physiology, 128: 471–479.
There are 1 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | FBD |
| Authors | |
| Publication Date | March 1, 2018 |
| Submission Date | April 19, 2017 |
| Published in Issue | Year 2018 Volume: 30 Issue: 1 |