Research Article
BibTex RIS Cite

Investigation of bovine coronavirus and bovine rotavirus by rapid diagnosis kit and RT-PCR in diarrheic calf feces

Year 2019, Volume: 3 Issue: 3, 57 - 63, 31.12.2019

Gülşah Uyunmaz Saklı Oya Bulut Mustafa Hasöksüz Hasan Hüseyin Hadimli

https://doi.org/10.30704/http-www-jivs-net.601639
Cited By: 5

Abstract

This study has investigated bovine coronavirus (BCoV) and bovine rotavirus (BRV), which are among the most important causes of diarrhea in calves leading to financial losses in Turkey and all over the world BCoV and BRV were detected by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), which is one of the most reliable method of diagnosis, The results obtained by RT-PCR were compared to the sensitivity of the commercial Rota-Corona Rapid Test Kits used by clinical veterinarians in fields. In this study, 96 fecal samples were examined from diarrheic calves in cattle farms in the cities of Konya and Afyon for BRV and BCoV firstly by BoviD-5 Ag rapid test kit, and then we applied the RT-PCR test. A comparison of the rapid test kit with the RT-PCR in terms of sensitivity and specificity revealed the 83% sensitivity and 100% specificity of the BRV and 7.6% sensitivity and 100% specificity of BCoV. In conclusion the practical and rapid diagnosis of the disease using of Rapid Diagnosis kit used by the clinician veterinarians may be useful, but the results must be interpreted with caution since the sensitivity of the test decreases due to the reduction in the number of viruses in the later stages of the infection.



 .











 

Keywords

BCoV BRV calf diarrhea Rapid Test RT-PCR

Supporting Institution

The Research Fund of Selcuk University.

References

  • Aich, P., Heather, L., Wilson, Radley, S., Kaushik, T., Andy, A., Potter Lorne, A., & Griebel P. (2007). Comparative analysis of innate immune responses following infection of newborn calves with bovine rotavirus and coronavirus. Journal of General Virology, 88, 2749-2761.
  • Alkan, F. (1998). Buzağı ishallerinde rotavirus ve coronavirusların rolü. AÜ Vet Fak Derg, 45, 29-37.
  • Al Mawly, J., Grinberg, A., Prattley, D., Moffat, J., & French, N. (2015). Prevalence of endemic enteropathogens of calves in New Zealand dairy farms. New Zealand Veterinary Journal, 63(3), 147-152.
  • Altug, N., Yuksek, N., Ozkan, C., Keles, I., Basbugan, Y., Agaoglu, Z.T., Kaya, A., & Akgul, Y. (2013). Neonatal Buzağı İshallerinin İmmunokromotografik Test Kitleri İle Hızlı Etiyolojik Teşhisi. Van Veterinary Journal, 24(3), 123-128.
  • Al-Yousif, Y., Anderson, J., Chard-Bergstrom, C., Bustamante, A., Muenzenberger, M., Austin, K., & Kapil, S. (2001). Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples. Clinical and Vaccine Immunology, 8(3), 496-498.
  • Asano, K.M., De Souza, S.P., De Barros, I.N., Ayres, G.R., Silva, S.O., Richtzenhain, L.J., & Brandao, P.E. (2010). Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus. Journal of Virological Methods, 169, 375-379.
  • Bartels, C.J., Holzhauer, M., Jorritsma, R., Swart, W.A., & Lam, T.J. (2010). Prevalence, prediction and risk factors of enteropathogens in normal and non-normal faeces of young Dutch dairy calves. Preventive Veterinary Medicine, 93(2), 162-169.
  • Boileau, M. J., & Kapil, S.(2010). Bovine coronavirus associated syndromes. Veterinary Clinics of North America: Food Animal Practice, 26, 123–146.
  • Bok, M., Miño, S., Rodriguez, D., Badaracco, A., Nuñes, I., Souza, S. P., Bilbao, G., Louge Uriarte E., Galarza, R., Vega, C., Odeon, A., Saif, L. J., & Parreño, V. (2015). Molecular and antigenic characterization of Bovine Coronavirus circulating in Argentinean cattle during 1994-2010. Veterinary Microbiology, 31, 221-229.
  • Chang, K. O., Parwani, A. V., Smith, D., & Saif, L. J. (1997). Detection of group B rotaviruses in fecal samples from diarrheic calves and adult cows and characterization of their VP7 genes. Journal Clinical Microbiology, 35, 2107-2110
  • Cho, K., Hasoksuz, M., Nielsen, P., Chang, K., Lathrop, S., & Saif, L. (2001). Cross-Protection Studies Between Respiratory And Calf Diarrhea And Winter Dysentery Coronavirus Strains In Calves An RT-PCR And Nested PCR For Their Detection. Archives of Virology, 146, 2401-2419.
  • Cho, Y.I., Sun, D., Cooper, V., Dewell, G., Schwartz, K., & Yoon, K.J. (2012). Evaluation of a commercial rapid test kit for detecting bovine enteric pathogens in feces. Journal of Veterinary Diagnostic Investigation, 24(3), 559-562.
  • Cho, Y. I., Han, J. I., Wang, C., Cooper, V., Schwartz, K., Engelken, T., & Yoon, K. J. (2013). Case-control study of microbiological etiology associated with calf diarrhea. Veterinary Microbiology, 166, 375-385.
  • Decaro, N., Elia, G., Campolo, M., Desario, C., Mari, V., Radogna, A., Colaianni, M. L., Cirone, F., Tempesta, M., & Buonavoglia, C. (2008). Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay. Journal Virology Methods, 151(2), 167–171.
  • De la Fuente, R., Garcia, A., & Ruiz-Santa-Quiteria, J.A. (1998). Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain. Preventive Veterinary Medicine, 36, 145-152.
  • Ellens, D. J., De Leeuw, P. W., & Straver, P. J. (1978). The detection of rotavirus specific antibody in colostrum and milk by ELISA. Annals of Veterinary Research, 9, 337-342.
  • Gulyaz ,V., Hasoksuz, M., & Ozkul, A. (2005).Türkiye’de yenidoğan ishalli buzağılarda ilk rotavirus izolasyonu. Pendik Veteriner Kontrol Araştırma Enstitüsü Dergisi, 35, 3-6.
  • Gulyaz, V., Turan, N., Ozdemir, S., & Gulacti, I. (2010). Yenidoğan ishalli buzağılarda bovine rotavirus enfeksiyonunun teşhisinde ELISA ve virus izolasyon metotlarının karşılaştırılması. Pendik Veteriner Mikrobiyoloji Dergisi, 37(1), 11-17.
  • Hasoksuz, M., Hoet, A., Loerch, S., Nielsen, P., Wittom, T., & Saif, L. (2002). Detection Of Respiratory And Enteric Shedding Of Bovine Coronaviruses In Cattle In An Ohio Feedlot. Journal of Veterinary Diagnostic Investigation, 14, 308-313.
  • Hasoksuz, M., Vlasova, A., & Saif, L. J. (2008). Detection of group 2a coronaviruses with emphasis on bovine and wild ruminant strains. Virus isolation and detection of antibody antigen, and nucleic acid. Methods in Moleculer Biology, 454, 43–59.
  • Izzo, M. M., Kirkland, P. D., Gu, X., Lele, Y., & Gunn, A. A., House, J. K. (2012). Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia. Australian Veterinary Journal, 90, 122-129.
  • Klein, D., Kern, A., & Lapan, G. (2009). Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves. The Veterinary Journal ,182, 484-486.
  • Mebus, C. A.; Underdahl, N. R.; Rhodes, M. B., & Twiehaus, M. J., (1969). "Calf Diarrhea (Scours): Reproduced with a Virus from a Field Outbreak" Historical Research Bulletins of the Nebraska Agricultural Experiment Station (1913-1993). 69.
  • Murphy, F. A., Gibbs, E. P. J., & Horzinek, M. C. (1999). Veterinary Virology. 3th ed. USA: A Division of Harcourt Brace Company, pp. 402-404.
  • Saif, L. J., Bohl, E. H., Theil, K.. W., Cross, R. F., & House, J. A. (1980). Rotavirus-like calicivirus-like, and 23-nm virus like particles associated with diarrhea in young pigs. Journal Clinical Microbiology,12(1), 105-111.
  • Thorns, C. J., Bell, M. M., Chasey, D., Chesham, J., & Roeder, P. L. (1992). Development of monoclonal antibody ELISA for simultaneous detection of bovine coronavirus, rotavirus serogroup A, and Escherichia coli K99 antigen in feces of calves. American Journal Veterinary Research, 53(1), 36-43.
  • Uhde, F. L., Kaufmann, T., Sager, H., Albini, S., Zanoni, R., Schelling, E., & Meylan, M. (2008). Prevalence of four enteropathogens in the faeces of young diarrheic dairy calves in Switzerland. Journal of the British Veterinary Association, 163(12):362-366.
  • WelIemans, G., & Opdenbosch, E. (1981). Postpartum antibody levels for rota, corona and BVD virus in cow’s milk. Vlaams Diergeneeskundig Tijdschrift, 50, 46-52. Woode, G. N., & Bridger, J. C. (1975). Viral enteritis of calves. Veterinary Record, 96, 85-88.

Year 2019, Volume: 3 Issue: 3, 57 - 63, 31.12.2019

Gülşah Uyunmaz Saklı Oya Bulut Mustafa Hasöksüz Hasan Hüseyin Hadimli

https://doi.org/10.30704/http-www-jivs-net.601639
Cited By: 5

Abstract

References

  • Aich, P., Heather, L., Wilson, Radley, S., Kaushik, T., Andy, A., Potter Lorne, A., & Griebel P. (2007). Comparative analysis of innate immune responses following infection of newborn calves with bovine rotavirus and coronavirus. Journal of General Virology, 88, 2749-2761.
  • Alkan, F. (1998). Buzağı ishallerinde rotavirus ve coronavirusların rolü. AÜ Vet Fak Derg, 45, 29-37.
  • Al Mawly, J., Grinberg, A., Prattley, D., Moffat, J., & French, N. (2015). Prevalence of endemic enteropathogens of calves in New Zealand dairy farms. New Zealand Veterinary Journal, 63(3), 147-152.
  • Altug, N., Yuksek, N., Ozkan, C., Keles, I., Basbugan, Y., Agaoglu, Z.T., Kaya, A., & Akgul, Y. (2013). Neonatal Buzağı İshallerinin İmmunokromotografik Test Kitleri İle Hızlı Etiyolojik Teşhisi. Van Veterinary Journal, 24(3), 123-128.
  • Al-Yousif, Y., Anderson, J., Chard-Bergstrom, C., Bustamante, A., Muenzenberger, M., Austin, K., & Kapil, S. (2001). Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples. Clinical and Vaccine Immunology, 8(3), 496-498.
  • Asano, K.M., De Souza, S.P., De Barros, I.N., Ayres, G.R., Silva, S.O., Richtzenhain, L.J., & Brandao, P.E. (2010). Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus. Journal of Virological Methods, 169, 375-379.
  • Bartels, C.J., Holzhauer, M., Jorritsma, R., Swart, W.A., & Lam, T.J. (2010). Prevalence, prediction and risk factors of enteropathogens in normal and non-normal faeces of young Dutch dairy calves. Preventive Veterinary Medicine, 93(2), 162-169.
  • Boileau, M. J., & Kapil, S.(2010). Bovine coronavirus associated syndromes. Veterinary Clinics of North America: Food Animal Practice, 26, 123–146.
  • Bok, M., Miño, S., Rodriguez, D., Badaracco, A., Nuñes, I., Souza, S. P., Bilbao, G., Louge Uriarte E., Galarza, R., Vega, C., Odeon, A., Saif, L. J., & Parreño, V. (2015). Molecular and antigenic characterization of Bovine Coronavirus circulating in Argentinean cattle during 1994-2010. Veterinary Microbiology, 31, 221-229.
  • Chang, K. O., Parwani, A. V., Smith, D., & Saif, L. J. (1997). Detection of group B rotaviruses in fecal samples from diarrheic calves and adult cows and characterization of their VP7 genes. Journal Clinical Microbiology, 35, 2107-2110
  • Cho, K., Hasoksuz, M., Nielsen, P., Chang, K., Lathrop, S., & Saif, L. (2001). Cross-Protection Studies Between Respiratory And Calf Diarrhea And Winter Dysentery Coronavirus Strains In Calves An RT-PCR And Nested PCR For Their Detection. Archives of Virology, 146, 2401-2419.
  • Cho, Y.I., Sun, D., Cooper, V., Dewell, G., Schwartz, K., & Yoon, K.J. (2012). Evaluation of a commercial rapid test kit for detecting bovine enteric pathogens in feces. Journal of Veterinary Diagnostic Investigation, 24(3), 559-562.
  • Cho, Y. I., Han, J. I., Wang, C., Cooper, V., Schwartz, K., Engelken, T., & Yoon, K. J. (2013). Case-control study of microbiological etiology associated with calf diarrhea. Veterinary Microbiology, 166, 375-385.
  • Decaro, N., Elia, G., Campolo, M., Desario, C., Mari, V., Radogna, A., Colaianni, M. L., Cirone, F., Tempesta, M., & Buonavoglia, C. (2008). Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay. Journal Virology Methods, 151(2), 167–171.
  • De la Fuente, R., Garcia, A., & Ruiz-Santa-Quiteria, J.A. (1998). Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain. Preventive Veterinary Medicine, 36, 145-152.
  • Ellens, D. J., De Leeuw, P. W., & Straver, P. J. (1978). The detection of rotavirus specific antibody in colostrum and milk by ELISA. Annals of Veterinary Research, 9, 337-342.
  • Gulyaz ,V., Hasoksuz, M., & Ozkul, A. (2005).Türkiye’de yenidoğan ishalli buzağılarda ilk rotavirus izolasyonu. Pendik Veteriner Kontrol Araştırma Enstitüsü Dergisi, 35, 3-6.
  • Gulyaz, V., Turan, N., Ozdemir, S., & Gulacti, I. (2010). Yenidoğan ishalli buzağılarda bovine rotavirus enfeksiyonunun teşhisinde ELISA ve virus izolasyon metotlarının karşılaştırılması. Pendik Veteriner Mikrobiyoloji Dergisi, 37(1), 11-17.
  • Hasoksuz, M., Hoet, A., Loerch, S., Nielsen, P., Wittom, T., & Saif, L. (2002). Detection Of Respiratory And Enteric Shedding Of Bovine Coronaviruses In Cattle In An Ohio Feedlot. Journal of Veterinary Diagnostic Investigation, 14, 308-313.
  • Hasoksuz, M., Vlasova, A., & Saif, L. J. (2008). Detection of group 2a coronaviruses with emphasis on bovine and wild ruminant strains. Virus isolation and detection of antibody antigen, and nucleic acid. Methods in Moleculer Biology, 454, 43–59.
  • Izzo, M. M., Kirkland, P. D., Gu, X., Lele, Y., & Gunn, A. A., House, J. K. (2012). Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia. Australian Veterinary Journal, 90, 122-129.
  • Klein, D., Kern, A., & Lapan, G. (2009). Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves. The Veterinary Journal ,182, 484-486.
  • Mebus, C. A.; Underdahl, N. R.; Rhodes, M. B., & Twiehaus, M. J., (1969). "Calf Diarrhea (Scours): Reproduced with a Virus from a Field Outbreak" Historical Research Bulletins of the Nebraska Agricultural Experiment Station (1913-1993). 69.
  • Murphy, F. A., Gibbs, E. P. J., & Horzinek, M. C. (1999). Veterinary Virology. 3th ed. USA: A Division of Harcourt Brace Company, pp. 402-404.
  • Saif, L. J., Bohl, E. H., Theil, K.. W., Cross, R. F., & House, J. A. (1980). Rotavirus-like calicivirus-like, and 23-nm virus like particles associated with diarrhea in young pigs. Journal Clinical Microbiology,12(1), 105-111.
  • Thorns, C. J., Bell, M. M., Chasey, D., Chesham, J., & Roeder, P. L. (1992). Development of monoclonal antibody ELISA for simultaneous detection of bovine coronavirus, rotavirus serogroup A, and Escherichia coli K99 antigen in feces of calves. American Journal Veterinary Research, 53(1), 36-43.
  • Uhde, F. L., Kaufmann, T., Sager, H., Albini, S., Zanoni, R., Schelling, E., & Meylan, M. (2008). Prevalence of four enteropathogens in the faeces of young diarrheic dairy calves in Switzerland. Journal of the British Veterinary Association, 163(12):362-366.
  • WelIemans, G., & Opdenbosch, E. (1981). Postpartum antibody levels for rota, corona and BVD virus in cow’s milk. Vlaams Diergeneeskundig Tijdschrift, 50, 46-52. Woode, G. N., & Bridger, J. C. (1975). Viral enteritis of calves. Veterinary Record, 96, 85-88.
There are 28 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Gülşah Uyunmaz Saklı 0000-0002-7807-9869

Oya Bulut This is me

Mustafa Hasöksüz

Hasan Hüseyin Hadimli

Publication Date December 31, 2019
Published in Issue Year 2019 Volume: 3 Issue: 3

Cite

APA Uyunmaz Saklı, G., Bulut, O., Hasöksüz, M., Hadimli, H. H. (2019). Investigation of bovine coronavirus and bovine rotavirus by rapid diagnosis kit and RT-PCR in diarrheic calf feces. Journal of Istanbul Veterinary Sciences, 3(3), 57-63. https://doi.org/10.30704/http-www-jivs-net.601639

Cited By

Development of a 1-step TaqMan real-time PCR method for detection of the Bovine Group A Rotavirus
Diagnostic Microbiology and Infectious Disease
https://doi.org/10.1016/j.diagmicrobio.2023.116081
Etiological Examination of Neonatal Calf Diarrhea Cases Detected in Burdur Region
Veterinary Journal of Mehmet Akif Ersoy University
https://doi.org/10.24880/maeuvfd.1120634
ELISA–based Point Prevalence of enteropathogens in diarrheic calves in Central Anatolia Region of Turkey
Revista Científica de la Facultad de Ciencias Veterinarias
https://doi.org/10.52973/rcfcv-e33249
Characterization of bovine rotavirus isolates from diarrheic calves in Türkiye
Molecular Biology Reports
https://doi.org/10.1007/s11033-022-08169-4
Neonatal buzağı ishal olgularında enterik virusların (BRV, BCoV, BVDV, BToV) çoklu enfeksiyonu
Journal of Research in Veterinary Medicine
https://doi.org/10.30782/jrvm.1166863

CC-BY
This journal is presented to the reader under  Creative Commons attribution 4.0 international  (CC-BY 4.0)