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Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot

Year 2021, Volume: 10 Issue: 1, 56 - 59, 22.06.2021

Serdal Tarhane

https://doi.org/10.31196/huvfd.881082

Abstract

European foulbrood disease caused by Melissoccocus plutonius which gram-positive bacterium that leads to larval deaths with its worldwide prevalence, paving the way for beehives to die away over time. It causes economic losses in honey production. Bee breeding and honey production are economically pivotal in Turkey, and it is vital to analyze the prevalence of this disease. This study aims to identify M. plutonius from the honeycombs suspected of European foulbrood and dead bee samples from the beehives (11 hives in total) that contain honeycombs collected from Cankırı, Hatay, Kars and Konya via conventional PCR method. In this study two samples DNA identified as M. plutonius (18% of total hives).

Keywords

European foulbrood Honey bees M. plutonius Turkey

Thanks

Great thanks to Kafkas University of Veterinary Faculty Microbiology Department for all of their support and contribution.

References

  • Alippi AMA, 1991: Comparison of Laboratory Techniques for the Detection of Significant Bacteria of the Honey bee, Apis mellifera, in Argentina. J. Apicult. Res, 30 (2), 75-80.
  • Arai R, Tominaga K, Wu M, Okura M, Ito K, Okamura, N, Takamatsu D, 2010: Diversity of Melissococcus plutonius from Honeybee Larvae in Japan and Experimental Reproduction of European Foulbrood with Cultured Atypical Isolates. PLoS ONE, 7(3), 1-10.
  • Barganska Z, Slebioda M, Namiesnik J, 2011: Determination of antibiotic residues in honey. Trends Analyt Chem, 30 (7), 1035-1041.
  • Belloy L, Imdorf A, Fries I, Forsgren E, Berthoud H, Kuhn R, Charriere JD, 2007: Spatial distribution of Melissococcus plutonius in adult honey bees collected from apiaries and colonies with and without symptoms of European foulbrood. Apidologie, 2 (38 ), 136-140.
  • Borum AE, Özakın C, Güneş E, Aydın L, Ülgen M, Çakmak İ, 2015: The Investigation by PCR and Culture Methods of Foulbrood Diseases in Honey Bees in South Marmara Region. Kafkas Univ Vet Fak Derg, 21 (1): 95-99.
  • Budge GE, Barrett B, Jones B, Pietravalle S, Marris G, Chantawannakul P, Thwaites R, Hall J, Cuthbertson AGS, Brown MA, 2010: The occurrence of Melissococcus plutonius in healthy colonies of Apis mellifera and the efficacy of European foulbrood control measures. J Invertebr Patho, 105 (2):164-70.
  • Forsgren E, Cassel L, Imdorf A, Fries I. 2005. Distribution of Melissococcus Plutonius in Honeybee Colonies With and Without Symptoms of European Foulbrood. Microb Ecol, 50 (3):369-74.
  • Govan VA, Brozel V, Allsopp MH, Davidson SA, 1998: PCR Detection Method for Rapid Identification of Melissococcus pluton in Honeybee Larvae. APPL. Environ Microbiol, 64 (5), 1983-1985.
  • Grossar D, Kilchenmannb V, Forsgren E, Charrière JD, Gauthierb L, Ma MC, Dietemann V, 2020: Putative determinants of virulence in Melissococcus plutonius, the bacterial. Virulance, 11(1), 554-567.
  • Jyothis PJ and Amritha VS, 2019: Survey and etiology of bacterial brood disease infecting Indian honey bees (Apis cerana indica F.) in Southern Kerala. J Apic Sci, 59 (4), 519-527.
  • Lewkowski O, and Erler S, 2018: Virulence of Melissococcus plutonius and secondary invaders associated with European foulbrood disease of the honey bee. Microbiology Open, 8 (3), 1-9.
  • McKEE BA, Goodman RD, Hortnitzk MA, 2004: The transmission of European foulbrood (Melissococcus plutonius) to artificially reared honey bee larvae (Apis mellifera). J Apic Sci, 43 (3): 93-100.
  • Tarımsal Ekonomi ve Politika Geliştirme Enstitüsü, 2020: Tarım Ürünleri Piyasaları, Arıcılık. Ocak 2020, No. Hü-01. Erişim: arastirma.tarimorman.gov.tr. Tibata VM, Junca H, Sanchez A, Corona M, Botero FA, Figuero J, 2018: Molecular detection of Melissococcus plutonius assessed in Africanized honey bee populations (Apis mellifera) in three regions of Colombia, J Apic Sci, 57 (3), 418-424.
  • Waite R, Jackson S, Thompson H, 2003: Preliminary investigations into possible resistance to oxytetracycline in Melissococcus plutonius, a pathogen of honeybee larvae. Lett Appl Microbiol, 36 (1), 20-24.

Türkiye’nin Bazı Bölgelerindeki Larva ve Yetişkin Bal Arılarında Avrupa Yavru Çürüklüğü Hastalığı Varlığının Konvansiyonel PCR Yöntemi ile Araştırılması

Year 2021, Volume: 10 Issue: 1, 56 - 59, 22.06.2021

Serdal Tarhane

https://doi.org/10.31196/huvfd.881082

Abstract

Avrupa yavru çürüklüğü hastalığı Melissoccocus plutonius Gram-pozitif bakteri etkenli bir hastalık olup dünya çapında yaygınlığıyla larva ölümlerine yol açmakta, arı kovanlarının zamanla sönmesine ve bal üretiminde ekonomik kayıplara yol açmaktadır. Özellikle arı yetiştiriciliği ve bal üretiminin ekonomik olarak önem arz ettiği ülkemizde bu hastalığın yaygınlığının araştırılması önemini korumaktadır. Bu araştırmada Çankırı, Hatay, Kars ve Konya illerinden toplanan Avrupa yavru çürüklüğü şüpheli petek ve bu petekleri içeren kovanların (toplam 11 kovan) önünden alınan ölü arı örneklerinden konvansiyonel PCR yöntemiyle bu hastalık etkeni M. plutonius’un varlığı tespit edilmeye çalışıldı. Alınan kovanlara ait örneklerin 2’sinde (toplam kovanların %18’inde) M. plutonius varlığı tespit edildi.

Keywords

Avrupa yavru çürüklüğü Bal arıları M. plutonius Türkiye

References

  • Alippi AMA, 1991: Comparison of Laboratory Techniques for the Detection of Significant Bacteria of the Honey bee, Apis mellifera, in Argentina. J. Apicult. Res, 30 (2), 75-80.
  • Arai R, Tominaga K, Wu M, Okura M, Ito K, Okamura, N, Takamatsu D, 2010: Diversity of Melissococcus plutonius from Honeybee Larvae in Japan and Experimental Reproduction of European Foulbrood with Cultured Atypical Isolates. PLoS ONE, 7(3), 1-10.
  • Barganska Z, Slebioda M, Namiesnik J, 2011: Determination of antibiotic residues in honey. Trends Analyt Chem, 30 (7), 1035-1041.
  • Belloy L, Imdorf A, Fries I, Forsgren E, Berthoud H, Kuhn R, Charriere JD, 2007: Spatial distribution of Melissococcus plutonius in adult honey bees collected from apiaries and colonies with and without symptoms of European foulbrood. Apidologie, 2 (38 ), 136-140.
  • Borum AE, Özakın C, Güneş E, Aydın L, Ülgen M, Çakmak İ, 2015: The Investigation by PCR and Culture Methods of Foulbrood Diseases in Honey Bees in South Marmara Region. Kafkas Univ Vet Fak Derg, 21 (1): 95-99.
  • Budge GE, Barrett B, Jones B, Pietravalle S, Marris G, Chantawannakul P, Thwaites R, Hall J, Cuthbertson AGS, Brown MA, 2010: The occurrence of Melissococcus plutonius in healthy colonies of Apis mellifera and the efficacy of European foulbrood control measures. J Invertebr Patho, 105 (2):164-70.
  • Forsgren E, Cassel L, Imdorf A, Fries I. 2005. Distribution of Melissococcus Plutonius in Honeybee Colonies With and Without Symptoms of European Foulbrood. Microb Ecol, 50 (3):369-74.
  • Govan VA, Brozel V, Allsopp MH, Davidson SA, 1998: PCR Detection Method for Rapid Identification of Melissococcus pluton in Honeybee Larvae. APPL. Environ Microbiol, 64 (5), 1983-1985.
  • Grossar D, Kilchenmannb V, Forsgren E, Charrière JD, Gauthierb L, Ma MC, Dietemann V, 2020: Putative determinants of virulence in Melissococcus plutonius, the bacterial. Virulance, 11(1), 554-567.
  • Jyothis PJ and Amritha VS, 2019: Survey and etiology of bacterial brood disease infecting Indian honey bees (Apis cerana indica F.) in Southern Kerala. J Apic Sci, 59 (4), 519-527.
  • Lewkowski O, and Erler S, 2018: Virulence of Melissococcus plutonius and secondary invaders associated with European foulbrood disease of the honey bee. Microbiology Open, 8 (3), 1-9.
  • McKEE BA, Goodman RD, Hortnitzk MA, 2004: The transmission of European foulbrood (Melissococcus plutonius) to artificially reared honey bee larvae (Apis mellifera). J Apic Sci, 43 (3): 93-100.
  • Tarımsal Ekonomi ve Politika Geliştirme Enstitüsü, 2020: Tarım Ürünleri Piyasaları, Arıcılık. Ocak 2020, No. Hü-01. Erişim: arastirma.tarimorman.gov.tr. Tibata VM, Junca H, Sanchez A, Corona M, Botero FA, Figuero J, 2018: Molecular detection of Melissococcus plutonius assessed in Africanized honey bee populations (Apis mellifera) in three regions of Colombia, J Apic Sci, 57 (3), 418-424.
  • Waite R, Jackson S, Thompson H, 2003: Preliminary investigations into possible resistance to oxytetracycline in Melissococcus plutonius, a pathogen of honeybee larvae. Lett Appl Microbiol, 36 (1), 20-24.
There are 14 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Serdal Tarhane 0000-0003-0806-7659

Publication Date June 22, 2021
Submission Date February 15, 2021
Acceptance Date April 12, 2021
Published in Issue Year 2021 Volume: 10 Issue: 1

Cite

APA Tarhane, S. (2021). Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot. Harran Üniversitesi Veteriner Fakültesi Dergisi, 10(1), 56-59. https://doi.org/10.31196/huvfd.881082
AMA Tarhane S. Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot. Harran Univ Vet Fak Derg. June 2021;10(1):56-59. doi:10.31196/huvfd.881082
Chicago Tarhane, Serdal. “Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey With Conventional PCR Methot”. Harran Üniversitesi Veteriner Fakültesi Dergisi 10, no. 1 (June 2021): 56-59. https://doi.org/10.31196/huvfd.881082.
EndNote Tarhane S (June 1, 2021) Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot. Harran Üniversitesi Veteriner Fakültesi Dergisi 10 1 56–59.
IEEE S. Tarhane, “Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot”, Harran Univ Vet Fak Derg, vol. 10, no. 1, pp. 56–59, 2021, doi: 10.31196/huvfd.881082.
ISNAD Tarhane, Serdal. “Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey With Conventional PCR Methot”. Harran Üniversitesi Veteriner Fakültesi Dergisi 10/1 (June 2021), 56-59. https://doi.org/10.31196/huvfd.881082.
JAMA Tarhane S. Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot. Harran Univ Vet Fak Derg. 2021;10:56–59.
MLA Tarhane, Serdal. “Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey With Conventional PCR Methot”. Harran Üniversitesi Veteriner Fakültesi Dergisi, vol. 10, no. 1, 2021, pp. 56-59, doi:10.31196/huvfd.881082.
Vancouver Tarhane S. Investigation the Exsistence of European Foulbrood Disease in Larvae and Adult Honeybees in Some Regions of Turkey with Conventional PCR Methot. Harran Univ Vet Fak Derg. 2021;10(1):56-9.
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