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Investigation of In Vitro antiproliferative activity properties of Spartium junceum L. (Spanish broom) against MDA-MB-231 and HepG2 cancer cell lines

Year 2023, Volume: 10 Issue: 3, 345 - 353, 27.08.2023

Fatma Tuğçe Gürağaç Dereli Senem Akkoç

https://doi.org/10.21448/ijsm.1182965

Abstract

Cancer is among the top global public health burdens leading to millions of deaths each year. The study aims to investigate the antiproliferative effect of Spartium junceum L. flowers on different cancer cell lines. The ethanolic extract of the flowers was prepared in the present study. Phytochemical analysis of the plant extract revealed the presence of several phenolic compounds such as cinnamic acid and its derivatives (chlorogenic, p-coumaric, ferulic acids), protocatechuic acid, epicatechin and luteolin. This extract was tested against human breast (MDA-MB-231) and liver (HepG2) cancer cell lines to find out its antiproliferative activity. It was determined that the extract was effective against both cell lines with IC50 values of 2.37 ± 0.47 and 0.98 ± 0.01 µL/mL for MDA-MB-231 and HepG2, respectively. Particularly, the extract was found to be more effective in the liver cancer cell line than the breast cancer cell line. All these obtained findings led us to believe that this medicinal plant could be a promising antiproliferative agent candidate for the treatment of human liver and breast cancers.

Keywords

Antiproliferative activity Cancer Phenolic compounds Spartium junceum

Supporting Institution

Süleyman Demirel University

Project Number

TSG-2021-8458

Thanks

SA is grateful to the Suleyman Demirel University Research Fund for their kind financial support with project number TSG-2021-8458.

References

  • Abusamra, Y.A., Scuruchi, M., Habibatni, S., Maammeri, Z., Benayache, S., D'Ascola, A., Avenoso, A., Campo, G.M., Spina, E. (2015). Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line. Pakistan Journal of Pharmaceutical Sciences, 28(4), 1225-1232.
  • Allugunti, V.R. (2022). Breast cancer detection based on thermographic images using machine learning and deep learning algorithms. International Journal of Engineering and Computer Science, 4(1), 49-56. https://doi.org/10.33545/26633582.2022.v4.i1a.68
  • Cerchiara, T., Straface, S.V., Chidichimo, G., Belsito, E.L., Liguori, A., Luppi, B., Bigucci, F., Zecchi, V. (2012). Spartium junceum aromatic water: chemical composition and antitumor activity. Natural Product Communications, 7(1), 137 140. https://doi.org/10.1177/1934578X12007001
  • Dennis, A., Ekpe, I., Yisa, B.N. (2022). A short Review of Recent Advances in Cancer Gene Therapy in relation to Chemotherapy for the Treatment of Breast and Prostate Cancer. Journal of Applied Health Sciences and Medicine, 2(2), 5-12.
  • Duman, R., Dogan, H.H., Karakis, H. (2019). Antiviral activity of Spartium junceum against herpes simplex virus type 1: An in-vitro study. International Journal of Pharmaceutical Sciences and Research, 10, 3274-3282. https://doi.org/10.13040/IJPSR.0975-8232
  • Horie, N., Hirabayashi, N., Takahashi, Y., Miyauchi, Y., Taguchi, H., Takeishi, K. (2005). Synergistic Effect of Green Tea Catechins on Cell Growth and Apoptosis Induction in Gastric Carcinoma Cells. Biological and Pharmaceutical Bulletin, 28(4), 574-579. https://doi.org/10.1248/bpb.28.574
  • Imran, M.A., Shahid, H. (2020). Anti-Cancer Compounds from Medicinal Plants: Isolation, Identification, and Characterization. International Journal of Biosciences, 17, 442-468.
  • Llovet, J.M., Castet, F., Heikenwalder, M., Maini, M.K., Mazzaferro, V., Pinato, D.J., Pikarsky, E., Zhu, A.X., Finn, R.S. (2022). Immunotherapies for hepatocellular carcinoma. Nature Reviews Clinical Oncology, 19(3), 151-172. https://doi.org/10.1038/s41571-021-00573-2
  • Menghini, L., Massarelli, P., Bruni, G., Pagiotti, R. (2006). Anti-inflammatory and analgesic effects of Spartium junceum L. flower extracts: a preliminary study. Journal of Medicinal Food, 9(3), 386-390. https://doi.org/10.1089/jmf.2006.9.386
  • Nadaf, M., Halimi, M., Mortazavi, M. (2012). Identification of nonpolar chemical composition Spartium junceum flower growing in Iran by GC-MS. Middle-East Journal of Scientific Research, 11(2), 221-224.
  • Nanni, V., Canuti, L., Gismondi, A., Canini, A. (2018). Hydroalcoholic extract of Spartium junceum L. flowers inhibits growth and melanogenesis in B16-F10 cells by inducing senescence. Phytomedicine, 46, 1-10. https://doi.org/10.1016/j.phymed.2018.06.008
  • Philips, B.J., Coyle, C.H., Morrisroe, S.N., Chancellor, M.B., Yoshimura, N. (2009). Induction of apoptosis in human bladder cancer cells by green tea catechins. Biomedical Research, 30(4), 207-215. https://doi.org/10.2220/biomedres.30.207
  • Rammal, H., Zahreddine, H., Hijazi, A. (2021). Chemical composition and antioxidant capacity of Spartium junceum grown in Lebanon. Journal of Medical Research and Health Sciences, 4(11), 1588-1591. https://doi.org/10.52845/JMRHS/2021-4-11-10
  • Sahayarayan, J.J., Rajan, K.S., Vidhyavathi, R., Nachiappan, M., Prabhu, D., Alfarraj, S., Arokiyaraj S., Daniel A.N. (2021). In-silico protein-ligand docking studies against the estrogen protein of breast cancer using pharmacophore based virtual screening approaches. Saudi Journal of Biological Sciences, 28(1), 400 407. https://doi.org/10.1016/j.sjbs.2020.10.023
  • Singh, B.N., Shankar, S., Srivastava, R.K. (2011). Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochemical Pharmacology, 82(12), 1807-1821. https://doi.org/10.1016/j.bcp.2011.07.093
  • van den Boogaard, W.M.C., Komninos, D.S.J., Vermeij, W.P. (2022). Chemotherapy Side-Effects: Not All DNA Damage Is Equal. Cancers, 14(3), 627. https://doi.org/10.3390/cancers14030627
  • Vale, J.Ad., Rodrigues, M.P., Lima, Â.M.A., Santiago, S.S., Lima, G.D.dA., Almeida, A.A., Oliveira, L.Ld, Bressan, G.C., Teixeira, R.R., Machado-Neves, M. (2022). Synthesis of cinnamic acid ester derivatives with antiproliferative and antimetastatic activities on murine melanoma cells. Biomedicine & Pharmacotherapy, 148, 112689. https://doi.org/10.1016/j.biopha.2022.112689
  • Yahiaoui, S., Kati, D.E., Ali, L.M., El Cheikh, K., Morére, A., Menut, C., Bachir-bey, M., Bettache, N. (2022). Assessment of antioxidant, antiproliferative, anti-inflammatory, and enzyme inhibition activities and UPLC-MS phenolic determination of Ficus carica latex. Industrial Crops and Products, 178, 114629. https://doi.org/10.1016/j.indcrop.2022.114629
  • Yeşilada, E., Tsuchiya, K., Takaishi, Y., Kawazoe, K. (2000a). Isolation and characterization of free radical scavenging flavonoid glycosides from the flowers of Spartium junceum by activity-guided fractionation. Journal of Ethnopharmacology, 73(3), 471-478. https://doi.org/10.1016/S0378-8741(00)00327-5
  • Yeşilada, E., Takaishi, Y., Fujita, T., Sezik, E. (2000b). Anti-ulcerogenic effects of Spartium junceum flowers on in vivo test models in rats. Journal of Ethnopharmacology, 70(3), 219-226. https://doi.org/10.1016/S0378-8741(99)00180-4
  • Zhang, S., Deng, N., Zheng, B., Li, T., Liu, R.H. (2022). The effect of in vitro gastrointestinal digestion on the phenolic profiles, bioactivities and bioaccessibility of Rhodiola. Food and Function, 13(10), 5752-5765. https://doi.org/10.1039/D2FO00469K
  • Zheng, R., Su, S., Li, J., Zhao, Z., Wie, J., Fu, X., Liu, R.H. (2017). Recovery of phenolics from the ethanolic extract of sugarcane (Saccharum officinarum L.) baggase and evaluation of the antioxidant and antiproliferative activities. Industrial Crops and Products, 107, 360-369. https://doi.org/10.1016/j.indcrop.2017.05.050
  • World Health Organization, date of access: 11.04.23. https://www.who.int/news-room/fact-sheets/detail/cancer
  • Wu, T., Duan, X., Hu, C., Wu, C., Chen, X., Huang, J., Liu, J., Cui, S. (2019). Synthesis and characterization of gold nanoparticles from Abies spectabilis extract and its anticancer activity on bladder cancer T24 cells. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 512-523. https://doi.org/10.1080/21691401.2018.1560305

Investigation of In Vitro antiproliferative activity properties of Spartium junceum L. (Spanish broom) against MDA-MB-231 and HepG2 cancer cell lines

Year 2023, Volume: 10 Issue: 3, 345 - 353, 27.08.2023

Fatma Tuğçe Gürağaç Dereli Senem Akkoç

https://doi.org/10.21448/ijsm.1182965

Abstract

Cancer is among the top global public health burdens leading to millions of deaths each year. The study aims to investigate the antiproliferative effect of Spartium junceum L. flowers on different cancer cell lines. The ethanolic extract of the flowers was prepared in the present study. Phytochemical analysis of the plant extract revealed the presence of several phenolic compounds such as cinnamic acid and its derivatives (chlorogenic, p-coumaric, ferulic acids), protocatechuic acid, epicatechin and luteolin. This extract was tested against human breast (MDA-MB-231) and liver (HepG2) cancer cell lines to find out its antiproliferative activity. It was determined that the extract was effective against both cell lines with IC50 values of 2.37 ± 0.47 and 0.98 ± 0.01 µL/mL for MDA-MB-231 and HepG2, respectively. Particularly, the extract was found to be more effective in the liver cancer cell line than the breast cancer cell line. All these obtained findings led us to believe that this medicinal plant could be a promising antiproliferative agent candidate for the treatment of human liver and breast cancers.

Keywords

Antiproliferative activity Cancer Phenolic compounds Spartium junceum

Project Number

TSG-2021-8458

References

  • Abusamra, Y.A., Scuruchi, M., Habibatni, S., Maammeri, Z., Benayache, S., D'Ascola, A., Avenoso, A., Campo, G.M., Spina, E. (2015). Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line. Pakistan Journal of Pharmaceutical Sciences, 28(4), 1225-1232.
  • Allugunti, V.R. (2022). Breast cancer detection based on thermographic images using machine learning and deep learning algorithms. International Journal of Engineering and Computer Science, 4(1), 49-56. https://doi.org/10.33545/26633582.2022.v4.i1a.68
  • Cerchiara, T., Straface, S.V., Chidichimo, G., Belsito, E.L., Liguori, A., Luppi, B., Bigucci, F., Zecchi, V. (2012). Spartium junceum aromatic water: chemical composition and antitumor activity. Natural Product Communications, 7(1), 137 140. https://doi.org/10.1177/1934578X12007001
  • Dennis, A., Ekpe, I., Yisa, B.N. (2022). A short Review of Recent Advances in Cancer Gene Therapy in relation to Chemotherapy for the Treatment of Breast and Prostate Cancer. Journal of Applied Health Sciences and Medicine, 2(2), 5-12.
  • Duman, R., Dogan, H.H., Karakis, H. (2019). Antiviral activity of Spartium junceum against herpes simplex virus type 1: An in-vitro study. International Journal of Pharmaceutical Sciences and Research, 10, 3274-3282. https://doi.org/10.13040/IJPSR.0975-8232
  • Horie, N., Hirabayashi, N., Takahashi, Y., Miyauchi, Y., Taguchi, H., Takeishi, K. (2005). Synergistic Effect of Green Tea Catechins on Cell Growth and Apoptosis Induction in Gastric Carcinoma Cells. Biological and Pharmaceutical Bulletin, 28(4), 574-579. https://doi.org/10.1248/bpb.28.574
  • Imran, M.A., Shahid, H. (2020). Anti-Cancer Compounds from Medicinal Plants: Isolation, Identification, and Characterization. International Journal of Biosciences, 17, 442-468.
  • Llovet, J.M., Castet, F., Heikenwalder, M., Maini, M.K., Mazzaferro, V., Pinato, D.J., Pikarsky, E., Zhu, A.X., Finn, R.S. (2022). Immunotherapies for hepatocellular carcinoma. Nature Reviews Clinical Oncology, 19(3), 151-172. https://doi.org/10.1038/s41571-021-00573-2
  • Menghini, L., Massarelli, P., Bruni, G., Pagiotti, R. (2006). Anti-inflammatory and analgesic effects of Spartium junceum L. flower extracts: a preliminary study. Journal of Medicinal Food, 9(3), 386-390. https://doi.org/10.1089/jmf.2006.9.386
  • Nadaf, M., Halimi, M., Mortazavi, M. (2012). Identification of nonpolar chemical composition Spartium junceum flower growing in Iran by GC-MS. Middle-East Journal of Scientific Research, 11(2), 221-224.
  • Nanni, V., Canuti, L., Gismondi, A., Canini, A. (2018). Hydroalcoholic extract of Spartium junceum L. flowers inhibits growth and melanogenesis in B16-F10 cells by inducing senescence. Phytomedicine, 46, 1-10. https://doi.org/10.1016/j.phymed.2018.06.008
  • Philips, B.J., Coyle, C.H., Morrisroe, S.N., Chancellor, M.B., Yoshimura, N. (2009). Induction of apoptosis in human bladder cancer cells by green tea catechins. Biomedical Research, 30(4), 207-215. https://doi.org/10.2220/biomedres.30.207
  • Rammal, H., Zahreddine, H., Hijazi, A. (2021). Chemical composition and antioxidant capacity of Spartium junceum grown in Lebanon. Journal of Medical Research and Health Sciences, 4(11), 1588-1591. https://doi.org/10.52845/JMRHS/2021-4-11-10
  • Sahayarayan, J.J., Rajan, K.S., Vidhyavathi, R., Nachiappan, M., Prabhu, D., Alfarraj, S., Arokiyaraj S., Daniel A.N. (2021). In-silico protein-ligand docking studies against the estrogen protein of breast cancer using pharmacophore based virtual screening approaches. Saudi Journal of Biological Sciences, 28(1), 400 407. https://doi.org/10.1016/j.sjbs.2020.10.023
  • Singh, B.N., Shankar, S., Srivastava, R.K. (2011). Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochemical Pharmacology, 82(12), 1807-1821. https://doi.org/10.1016/j.bcp.2011.07.093
  • van den Boogaard, W.M.C., Komninos, D.S.J., Vermeij, W.P. (2022). Chemotherapy Side-Effects: Not All DNA Damage Is Equal. Cancers, 14(3), 627. https://doi.org/10.3390/cancers14030627
  • Vale, J.Ad., Rodrigues, M.P., Lima, Â.M.A., Santiago, S.S., Lima, G.D.dA., Almeida, A.A., Oliveira, L.Ld, Bressan, G.C., Teixeira, R.R., Machado-Neves, M. (2022). Synthesis of cinnamic acid ester derivatives with antiproliferative and antimetastatic activities on murine melanoma cells. Biomedicine & Pharmacotherapy, 148, 112689. https://doi.org/10.1016/j.biopha.2022.112689
  • Yahiaoui, S., Kati, D.E., Ali, L.M., El Cheikh, K., Morére, A., Menut, C., Bachir-bey, M., Bettache, N. (2022). Assessment of antioxidant, antiproliferative, anti-inflammatory, and enzyme inhibition activities and UPLC-MS phenolic determination of Ficus carica latex. Industrial Crops and Products, 178, 114629. https://doi.org/10.1016/j.indcrop.2022.114629
  • Yeşilada, E., Tsuchiya, K., Takaishi, Y., Kawazoe, K. (2000a). Isolation and characterization of free radical scavenging flavonoid glycosides from the flowers of Spartium junceum by activity-guided fractionation. Journal of Ethnopharmacology, 73(3), 471-478. https://doi.org/10.1016/S0378-8741(00)00327-5
  • Yeşilada, E., Takaishi, Y., Fujita, T., Sezik, E. (2000b). Anti-ulcerogenic effects of Spartium junceum flowers on in vivo test models in rats. Journal of Ethnopharmacology, 70(3), 219-226. https://doi.org/10.1016/S0378-8741(99)00180-4
  • Zhang, S., Deng, N., Zheng, B., Li, T., Liu, R.H. (2022). The effect of in vitro gastrointestinal digestion on the phenolic profiles, bioactivities and bioaccessibility of Rhodiola. Food and Function, 13(10), 5752-5765. https://doi.org/10.1039/D2FO00469K
  • Zheng, R., Su, S., Li, J., Zhao, Z., Wie, J., Fu, X., Liu, R.H. (2017). Recovery of phenolics from the ethanolic extract of sugarcane (Saccharum officinarum L.) baggase and evaluation of the antioxidant and antiproliferative activities. Industrial Crops and Products, 107, 360-369. https://doi.org/10.1016/j.indcrop.2017.05.050
  • World Health Organization, date of access: 11.04.23. https://www.who.int/news-room/fact-sheets/detail/cancer
  • Wu, T., Duan, X., Hu, C., Wu, C., Chen, X., Huang, J., Liu, J., Cui, S. (2019). Synthesis and characterization of gold nanoparticles from Abies spectabilis extract and its anticancer activity on bladder cancer T24 cells. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 512-523. https://doi.org/10.1080/21691401.2018.1560305
There are 24 citations in total.

Details

Primary Language English
Subjects Plant Biochemistry, Pharmacology and Pharmaceutical Sciences
Journal Section Articles
Authors

Fatma Tuğçe Gürağaç Dereli 0000-0002-7554-733X

Senem Akkoç 0000-0002-1260-9425

Project Number TSG-2021-8458
Early Pub Date July 31, 2023
Publication Date August 27, 2023
Submission Date October 1, 2022
Published in Issue Year 2023 Volume: 10 Issue: 3

Cite

APA Gürağaç Dereli, F. T., & Akkoç, S. (2023). Investigation of In Vitro antiproliferative activity properties of Spartium junceum L. (Spanish broom) against MDA-MB-231 and HepG2 cancer cell lines. International Journal of Secondary Metabolite, 10(3), 345-353. https://doi.org/10.21448/ijsm.1182965
International Journal of Secondary Metabolite

e-ISSN: 2148-6905