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The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season

Year 2018, Volume: 1 Issue: 2, 44 - 46, 22.12.2018

Ayhan Ata Muhammed Enes Inanc Sukru Gungor

Abstract

Bu çalışmanın amacı, sezon dışı dönemde değişik oranlardaki α-tokoferolün koç spermasının dondurulabilirliğine etkisini incelemektir. Çalışmada 4 adet Merinos koçu (2- 3 yaşlarında ve Bademli/Burdur’da özel bir işletmede) kullanıldı. Elektroejakülatör kullanılarak koçlardan alınan ejakulatlardan > %80 motilite ve 1.5 x109 spermatozoa/ml üzerinde yoğunluğu bulunan ejakulatlar birleştirildi. Birleştirilen ejakulat beş eşit gruba ayrılarak 0 mg (kontrol), 100 µM, 200 µM, 400µM or 800 µM α-tokoferol içeren tris ile sulandırıldı, 4 C⁰’de ekibrilasyon yapılarak mini payetlerde donduruldu ve sıvı azotta depo edildi. Payetler daha sonda spermatolojik analizler için 37 C⁰’de 30 sn çözdürüldü. Kontrol grubuna (37.50±7.90; 26.85±3.17) göre sırası ile en yüksek motilite ve membran bütünlüğü 100 µM grupta (47.77±4.40; 34.83±2.64) tespit edildi (p<0.05). Ayrıca gruplar arası anormal sperma muayene sonuçlarında istatistiksel bir farklılık bulunmadı (p>0.05). Sonuç olarak, 100 µM α-tokoferol koç sperma dondurma sulandırıcılarına kontrol grubu ile karşılaştırıldığında sezon dışında motilite ve membran bütünlüğünü iyileştirdiği için eklenmelidir. 

Keywords

α-tokoferol koç sperması dondurulabilirliliği sezon dışı motilite membran bütünlüğü anormal spermatozoa oranı

References

  • Lemma A. 2011. Effect of Cryopreservation on Sperm Quality and Fertility, Artificial Insemination in Farm Animals. Dr. Milad Manafi (Ed.), ISBN: 978-953-307-3125.
  • Satorre MM., Breininger E., Beconi M.T., Beorlegui NB. 2007. α-tocopherol modifies tyrosine phosphorylation and capacitation-like state of cryopreserved porcine sperm, Theriogenology, 68: 958–965.
  • Parks JE., Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38: 209–22.
  • Bennetts LE, Aitken RJ. 2005. A comparative study of oxidative DNA damage in mammalian spermatozoa. Mol. Reprod. Dev., 71:77–87.
  • Yousef MI, Abdallah GA, Kamel KI. 2003. Effect of ascorbic acid and Vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim Reprod Sci., 76: 99-111.
  • Pena FJ, Johannisson A, Wallgren M, Rodriguez Martinez H. 2003. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim Reprod Sci.,78: 85–98. PMID: 12753785.
  • Maia MS, Bicudo SD, Azevedo HC, Sicherle CC, Sousal DB, Rodello L. 2009. Motility and viability of ram sperm cryopreserved in a tris-egg yolk extender suppemented with anti-oxidants. Small Ruminant Res., 85: 85–90.
  • Suleiman SA, Ali ME, Zaki ZM, El-Malik EM, Nasr MA. 1996. Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl.,17: 530-537.
  • Kulaksız R. 2009. Farklı Antioksidanlar eklenmiş sulandırıcılarla dondurulmuş Saanen teke spermasının in vitro değerlendirilmesi. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü. Doktora tezi.
  • Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Kumar BM, Balasubramanian, Rho GJ. 2009. Effect of α-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology, 58: 181–189.
  • Daramola J, Adekunle E, Oke O, Ogundele O, Saanu E, Odeyemi A. 2016. Effect of vitamin E on sperm and oxidative stress parameters of west African Dwarf Goat Bucks. Tropical and Subtropical Agroecosystems, 19: 151158.
  • Sinclair S. 2000. Male infertility: Nutritional and environmental considerations. Alternatives Med Rev, 5: 28–38.
  • Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. 1997. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril, 68: 519-24.
  • Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl, 26: 349-53.

Year 2018, Volume: 1 Issue: 2, 44 - 46, 22.12.2018

Ayhan Ata Muhammed Enes Inanc Sukru Gungor

Abstract

References

  • Lemma A. 2011. Effect of Cryopreservation on Sperm Quality and Fertility, Artificial Insemination in Farm Animals. Dr. Milad Manafi (Ed.), ISBN: 978-953-307-3125.
  • Satorre MM., Breininger E., Beconi M.T., Beorlegui NB. 2007. α-tocopherol modifies tyrosine phosphorylation and capacitation-like state of cryopreserved porcine sperm, Theriogenology, 68: 958–965.
  • Parks JE., Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38: 209–22.
  • Bennetts LE, Aitken RJ. 2005. A comparative study of oxidative DNA damage in mammalian spermatozoa. Mol. Reprod. Dev., 71:77–87.
  • Yousef MI, Abdallah GA, Kamel KI. 2003. Effect of ascorbic acid and Vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim Reprod Sci., 76: 99-111.
  • Pena FJ, Johannisson A, Wallgren M, Rodriguez Martinez H. 2003. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim Reprod Sci.,78: 85–98. PMID: 12753785.
  • Maia MS, Bicudo SD, Azevedo HC, Sicherle CC, Sousal DB, Rodello L. 2009. Motility and viability of ram sperm cryopreserved in a tris-egg yolk extender suppemented with anti-oxidants. Small Ruminant Res., 85: 85–90.
  • Suleiman SA, Ali ME, Zaki ZM, El-Malik EM, Nasr MA. 1996. Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl.,17: 530-537.
  • Kulaksız R. 2009. Farklı Antioksidanlar eklenmiş sulandırıcılarla dondurulmuş Saanen teke spermasının in vitro değerlendirilmesi. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü. Doktora tezi.
  • Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Kumar BM, Balasubramanian, Rho GJ. 2009. Effect of α-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology, 58: 181–189.
  • Daramola J, Adekunle E, Oke O, Ogundele O, Saanu E, Odeyemi A. 2016. Effect of vitamin E on sperm and oxidative stress parameters of west African Dwarf Goat Bucks. Tropical and Subtropical Agroecosystems, 19: 151158.
  • Sinclair S. 2000. Male infertility: Nutritional and environmental considerations. Alternatives Med Rev, 5: 28–38.
  • Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. 1997. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril, 68: 519-24.
  • Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl, 26: 349-53.
There are 14 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Articles
Authors

Ayhan Ata This is me

Muhammed Enes Inanc

Sukru Gungor This is me

Publication Date December 22, 2018
Published in Issue Year 2018 Volume: 1 Issue: 2

Cite

APA Ata, A., Inanc, M. E., & Gungor, S. (2018). The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research, 1(2), 44-46.
AMA Ata A, Inanc ME, Gungor S. The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. IJVAR. December 2018;1(2):44-46.
Chicago Ata, Ayhan, Muhammed Enes Inanc, and Sukru Gungor. “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”. International Journal of Veterinary and Animal Research 1, no. 2 (December 2018): 44-46.
EndNote Ata A, Inanc ME, Gungor S (December 1, 2018) The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. International Journal of Veterinary and Animal Research 1 2 44–46.
IEEE A. Ata, M. E. Inanc, and S. Gungor, “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”, IJVAR, vol. 1, no. 2, pp. 44–46, 2018.
ISNAD Ata, Ayhan et al. “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”. International Journal of Veterinary and Animal Research 1/2 (December 2018), 44-46.
JAMA Ata A, Inanc ME, Gungor S. The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. IJVAR. 2018;1:44–46.
MLA Ata, Ayhan et al. “The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season”. International Journal of Veterinary and Animal Research, vol. 1, no. 2, 2018, pp. 44-46.
Vancouver Ata A, Inanc ME, Gungor S. The Effect Of Α-Tocopherol On Ram Semen Freezability In Non-Breeding Season. IJVAR. 2018;1(2):44-6.