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ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ

Year 2007, Volume: 33 Issue: 1, 11 - 21, 26.11.2012

Abstract

Sunulan çalışmada spermaya gliserolün farklı ısılarda eklenmesi ve spermanın farklı hızlarda
soğutulmasının, koçlarda donma sonrası spermatolojik özelliklere nasıl etkilediği araştırıldı. Üç-beş yaşlarında
10 baş Kıvırcık ırkı koçun spermaları alınarak pooling yapıldı ve sulandırılarak payetlerde donduruldu.
Spermanın tek aşamada (30°C) veya iki aşamada (30°C’de ve 5°C’de) sulandırılması ve 5°C’ye yavaş
( < 0.2°C/dakika) ve iki-kademeli (30°C’den 16°C’ye < 2°C/dakika ve 16°C’den 5°C’ye < 0.2/dakika) soğutma
tekniklerine göre 6 grup oluşturuldu: Sperma 30°C’de gliserol içermeyen sulandırıcı A ile sulandırıldı ve
5°C’ye yavaş (1. grup) ve iki-kademeli (2. grup) soğutuldu ve B sulandırıcısı ilave edildi. Yine sperma
30°C’de %2 gliserol içeren sulandırıcı A ile sulandırıldı ve 5°C’ye yavaş (3. grup) ve iki-kademeli (4. grup)
soğutuldu. Diğer gruplarda, sulandırma işlemi sulandırıcı A ve B ile 30°C’de tamamlandı ve 5°C’ye yavaş (5.
grup) ve iki-kademeli (6. grup) soğutuldu. Örnekler, ekilibrasyon sonrası payetlerde donduruldu.
Spermatolojik incelemeler (motilite, akrozomal ve toplam morfolojik bozukluklar), sulandırma sonrası (1.
aşama), 5°C’ ye soğutma sonrası (2. aşama), ekilibrasyon sonrası (3. aşama) ve eritme sonrası (4. aşama)
gerçekleştirildi.
Sunulan çalışmada (1) 1:1/2 sulandırma oranında 30°C’den 5°C’ye soğuma aşamasında ortamda
gliserol varlığının, eritme sonrası motiliteye zarar verdiği, (2) bu zararın iki-kademeli soğutma tekniği ile
azaldığı sonuçlarına varılmıştır.

References

  • Abdelhakeam, A.A., Graham, E.F and Vazquez, I.A. Studies on the presence and absence of glycerol in unfrozen ram semen: Fertility trials and the effects of dilution methods on freezing ram semen in the absence of glycerol. Cryobiology, 1991; 28: 36-42. 2
  • Ak, K., Ak, S., Gürel, A., Hasöksüz, M ve Baran, A. Koçlarda mycoplasma agalactiae’nin spermatolojik özelliklere etkisi üzerine deneysel araştırmalar. Pendik Vet Mikrobiyol Derg, 1995; 26 (2): 139-155. 6
  • Anel, L., dePaz, P., Alverez, M., Chomoro., C.A., Boixo, J.C., Manso, A et al. Field and invitro assay of three methods for freezing ram semen. Theriogenology, 2003: 60: 1293- 1308. 10
  • Colas, G. Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep frozen ram semen. J Reprod Fertil, 1975; 42: 277-285. 19
  • Correa, J.R., Rodriquez, M.C., Patterson, D.J and Zavos, P.M. Thawing and processing of cryopreserved bovine spermatozoa at various temperatures and their effects on sperm viability, osmotic shock and sperm membrane functional integrity. Theriogenology, 1996; 46 (3): 413-420. 20
  • Critser, J.K., Huse-Benda, A.R., Aaker, D.V., Arneson, B.W and Ball, G.D. Cryopreservation of human spermatozoon ΠІ:The effect of cryoprotectants on motility. Fertil Steril, 1988; 50 (2): 314-320. 21
  • Curry, M.R., Millar, J.D and Watson, P.F. Calculated optimal cooling Rates for Ram and Human Sperm Cryopreservation fail to conform with Empirical observations. Biol Reprod, 1994; 51: 1014-1021. 22
  • Curry, M.R., Kleinhans, F.W and Watson, P.F. Measurement of water permeability of the membrane of boar, ram and rabbit spermatozoon using concentration dependent self quenching of an entrapped fluorophore. Cryobiology, 2000; 41: 167-173. 24
  • D’Allessandro, A.G.D., Martemucci, G., Collanna, M.A and Bellini, A. Post thaw survival of ram spermatozoon and fertility after insemination as affected by prefreezing sperm concentration and extender composition. Theriogenology, 2001; 55: 1159-1170. 25
  • D’Alessandro, A.G and Martemucci,G. Post-thaw survival and acrosome integrity of spermatozoa of Leccese rams frozen in different seasons with a milk-egg yolk extender.Italian J Anim Sci, 2005; 4: 139-148. 26
  • El-Alamy, M.A and Foote, R.H.. Freezaability of spermatozoon from Finn and Dorset rams in multiple extenders. Anim Reprod Sci, 2001; 65: 245-254. 31
  • Evans, G and Maxwell, W.M.C. Salamon’s Artificial Insemination of Sheep and Goats. Sydney; Butterworths, 1987; 19: 91-141. 34
  • Fahy, G.M. The relevance of cryoprotectant toxicity to cryobiology Cryobiology, 1986; 23: 1-13. 35
  • Fiser, P.S and Fairfull, R.W. The effect of glycerol concentration and cooling velocity on cryosurvival of ram spermatozoon frozen in straws. Cryobiology, 1984; 21: 542-551. 38
  • Fiser, P.S and Fairfull, R.W. Combined effects of glycerol concentration, cooling velocity and osmolality of skim milk diluents on cryopreservation of ram spermatozoon. Theriogenology, 1986; 25 (3): 473-484. 39
  • Fiser, P.S and Fairfull, R.W. The effects of rapid cooling (cold shock) of ram semen, photoperiod and egg yolk in diluents on the survival of spermatozoon before and after freezing. Cryobiology, 1986; 23: 518-524. 40
  • Gil, J., Rodriguez-Irazoqui, M., Söderquist, L and Rodriguez-Martinez, H. Influence of centrifugation or low extension rates prefreezing on the fertility of ram semen after cervical insemination. Theriogenology, 2002; 57: 1781-1792. 45
  • Gil, J., Rodriguez-Irazoqui, M., Lundeheim, N., Söderquist, L and Rodriguez- Martinez, H. Fertility of ram semen frozen in Bioexcell® and used for cervical artificial insemination. Theriogenology, 2003; 59: 1157-1170. 46
  • Gil, J., Lundeheim, N., Söderquist, L and Rodriguez-Martinez, H. Influence of extender, temperature and addition of glycerol on post thaw sperm parameters in ram semen. Theriogenology, 2003; 59: 1241-1255. 47
  • Gökçen, H ve Aştı, R.N. Sıvı azot buharında dondurma yönteminin çeşitli evrelerinde, koç spermatozoitlerindeki akrozom bozukluklarının saptanması. A Ü Vet Fak Derg, 1980; 27 (3- 4): 501-514. 51
  • Jones, R.C. The use of dimethyl sulfoxide, glycerol and reconstituted skim milk for the preservation of ram spermatozoon. ІІ.: The influence of diluent composition and processing time during freezing to –79ºC with dimethyl sulfoxide or glycerol or both compounds. Aust J Biol Sci, 1965; 18: 887-899. 62
  • Mann,T and White, L.G. Glycerol metabolism by spermatozoon. Biochem, 1957; 65 (4): 634-639. 68
  • Maxwell, W.M.C and Watson, P.F. Recent progress in the preservation of ram semen. Anim Reprod Sci, 1996; 42: 55-65. 73
  • Noiles, E.E., Bailey, J and Storey, B.T. Temperature dependence of the water permeability, LP of murine sperm shows a discontinuity between 4°C and 0°C. Cryobiology, 1995; 32: 220-238. 90
  • Ollero, M., Perez-pe, R., Muino-Blanco, T and Cebrian-Perez, J.A. Improvement of ram sperm cryopreservation protocols assessed by sperm quality parameters and heterogeneity analysis. Cryobiology, 1998; 37: 1-12. 95
  • Salamon, S and Maxwell, W.M.C. Frozen storage of ram semen. І: processing, freezing, thawing and fertility after cervical insemination. Anim Reprod Sci, 1995; 37: 185-249. 113
  • Salamon, S and Maxwell, W.M.C. Frozen storage of ram semen. Π: Causes of low fertility after cervical insemination and method of improvement. Anim Reprod Sci, 1995; 38: 1-36. 114
  • Salamon, S and Maxwell, W.M.C. Storage of ram semen. Anim Reprod Sci, 2000; 62: 77- 111. 115
  • Sayre, B.L and Lewis, G.S. Fertility and ovum fertilization rate after laparosaopic or transcervikal intrauterine artificial insemination of oxytocin treated ewes. Theriogenology, 1997; 48: 267-275. 116
  • Slavik, T. Effect of glycerol on the penetrating ability of fresh ram spermatozoon with zona- free hamster eggs. J Reprod Fertil, 1987; 79: 99-103. 120
  • Watson, P.F and Martin, I.C. Artificial insemination of sheep : the effect of semen diluents containing egg yolk on the fertility of ram semen. Theriogenology, 1976; 6: 559-564. 131
  • White, I.G. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev, 1993; 5: 639-658. 134
Year 2007, Volume: 33 Issue: 1, 11 - 21, 26.11.2012

Abstract

References

  • Abdelhakeam, A.A., Graham, E.F and Vazquez, I.A. Studies on the presence and absence of glycerol in unfrozen ram semen: Fertility trials and the effects of dilution methods on freezing ram semen in the absence of glycerol. Cryobiology, 1991; 28: 36-42. 2
  • Ak, K., Ak, S., Gürel, A., Hasöksüz, M ve Baran, A. Koçlarda mycoplasma agalactiae’nin spermatolojik özelliklere etkisi üzerine deneysel araştırmalar. Pendik Vet Mikrobiyol Derg, 1995; 26 (2): 139-155. 6
  • Anel, L., dePaz, P., Alverez, M., Chomoro., C.A., Boixo, J.C., Manso, A et al. Field and invitro assay of three methods for freezing ram semen. Theriogenology, 2003: 60: 1293- 1308. 10
  • Colas, G. Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep frozen ram semen. J Reprod Fertil, 1975; 42: 277-285. 19
  • Correa, J.R., Rodriquez, M.C., Patterson, D.J and Zavos, P.M. Thawing and processing of cryopreserved bovine spermatozoa at various temperatures and their effects on sperm viability, osmotic shock and sperm membrane functional integrity. Theriogenology, 1996; 46 (3): 413-420. 20
  • Critser, J.K., Huse-Benda, A.R., Aaker, D.V., Arneson, B.W and Ball, G.D. Cryopreservation of human spermatozoon ΠІ:The effect of cryoprotectants on motility. Fertil Steril, 1988; 50 (2): 314-320. 21
  • Curry, M.R., Millar, J.D and Watson, P.F. Calculated optimal cooling Rates for Ram and Human Sperm Cryopreservation fail to conform with Empirical observations. Biol Reprod, 1994; 51: 1014-1021. 22
  • Curry, M.R., Kleinhans, F.W and Watson, P.F. Measurement of water permeability of the membrane of boar, ram and rabbit spermatozoon using concentration dependent self quenching of an entrapped fluorophore. Cryobiology, 2000; 41: 167-173. 24
  • D’Allessandro, A.G.D., Martemucci, G., Collanna, M.A and Bellini, A. Post thaw survival of ram spermatozoon and fertility after insemination as affected by prefreezing sperm concentration and extender composition. Theriogenology, 2001; 55: 1159-1170. 25
  • D’Alessandro, A.G and Martemucci,G. Post-thaw survival and acrosome integrity of spermatozoa of Leccese rams frozen in different seasons with a milk-egg yolk extender.Italian J Anim Sci, 2005; 4: 139-148. 26
  • El-Alamy, M.A and Foote, R.H.. Freezaability of spermatozoon from Finn and Dorset rams in multiple extenders. Anim Reprod Sci, 2001; 65: 245-254. 31
  • Evans, G and Maxwell, W.M.C. Salamon’s Artificial Insemination of Sheep and Goats. Sydney; Butterworths, 1987; 19: 91-141. 34
  • Fahy, G.M. The relevance of cryoprotectant toxicity to cryobiology Cryobiology, 1986; 23: 1-13. 35
  • Fiser, P.S and Fairfull, R.W. The effect of glycerol concentration and cooling velocity on cryosurvival of ram spermatozoon frozen in straws. Cryobiology, 1984; 21: 542-551. 38
  • Fiser, P.S and Fairfull, R.W. Combined effects of glycerol concentration, cooling velocity and osmolality of skim milk diluents on cryopreservation of ram spermatozoon. Theriogenology, 1986; 25 (3): 473-484. 39
  • Fiser, P.S and Fairfull, R.W. The effects of rapid cooling (cold shock) of ram semen, photoperiod and egg yolk in diluents on the survival of spermatozoon before and after freezing. Cryobiology, 1986; 23: 518-524. 40
  • Gil, J., Rodriguez-Irazoqui, M., Söderquist, L and Rodriguez-Martinez, H. Influence of centrifugation or low extension rates prefreezing on the fertility of ram semen after cervical insemination. Theriogenology, 2002; 57: 1781-1792. 45
  • Gil, J., Rodriguez-Irazoqui, M., Lundeheim, N., Söderquist, L and Rodriguez- Martinez, H. Fertility of ram semen frozen in Bioexcell® and used for cervical artificial insemination. Theriogenology, 2003; 59: 1157-1170. 46
  • Gil, J., Lundeheim, N., Söderquist, L and Rodriguez-Martinez, H. Influence of extender, temperature and addition of glycerol on post thaw sperm parameters in ram semen. Theriogenology, 2003; 59: 1241-1255. 47
  • Gökçen, H ve Aştı, R.N. Sıvı azot buharında dondurma yönteminin çeşitli evrelerinde, koç spermatozoitlerindeki akrozom bozukluklarının saptanması. A Ü Vet Fak Derg, 1980; 27 (3- 4): 501-514. 51
  • Jones, R.C. The use of dimethyl sulfoxide, glycerol and reconstituted skim milk for the preservation of ram spermatozoon. ІІ.: The influence of diluent composition and processing time during freezing to –79ºC with dimethyl sulfoxide or glycerol or both compounds. Aust J Biol Sci, 1965; 18: 887-899. 62
  • Mann,T and White, L.G. Glycerol metabolism by spermatozoon. Biochem, 1957; 65 (4): 634-639. 68
  • Maxwell, W.M.C and Watson, P.F. Recent progress in the preservation of ram semen. Anim Reprod Sci, 1996; 42: 55-65. 73
  • Noiles, E.E., Bailey, J and Storey, B.T. Temperature dependence of the water permeability, LP of murine sperm shows a discontinuity between 4°C and 0°C. Cryobiology, 1995; 32: 220-238. 90
  • Ollero, M., Perez-pe, R., Muino-Blanco, T and Cebrian-Perez, J.A. Improvement of ram sperm cryopreservation protocols assessed by sperm quality parameters and heterogeneity analysis. Cryobiology, 1998; 37: 1-12. 95
  • Salamon, S and Maxwell, W.M.C. Frozen storage of ram semen. І: processing, freezing, thawing and fertility after cervical insemination. Anim Reprod Sci, 1995; 37: 185-249. 113
  • Salamon, S and Maxwell, W.M.C. Frozen storage of ram semen. Π: Causes of low fertility after cervical insemination and method of improvement. Anim Reprod Sci, 1995; 38: 1-36. 114
  • Salamon, S and Maxwell, W.M.C. Storage of ram semen. Anim Reprod Sci, 2000; 62: 77- 111. 115
  • Sayre, B.L and Lewis, G.S. Fertility and ovum fertilization rate after laparosaopic or transcervikal intrauterine artificial insemination of oxytocin treated ewes. Theriogenology, 1997; 48: 267-275. 116
  • Slavik, T. Effect of glycerol on the penetrating ability of fresh ram spermatozoon with zona- free hamster eggs. J Reprod Fertil, 1987; 79: 99-103. 120
  • Watson, P.F and Martin, I.C. Artificial insemination of sheep : the effect of semen diluents containing egg yolk on the fertility of ram semen. Theriogenology, 1976; 6: 559-564. 131
  • White, I.G. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev, 1993; 5: 639-658. 134
There are 32 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Süleyman Bacınoğlu This is me

Ümit Cirit This is me

Zakeriya Nur This is me

Kemal Ak

Publication Date November 26, 2012
Published in Issue Year 2007 Volume: 33 Issue: 1

Cite

APA Bacınoğlu, S., Cirit, Ü., Nur, Z., Ak, K. (2012). ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 33(1), 11-21. https://doi.org/10.16988/iuvfd.84406
AMA Bacınoğlu S, Cirit Ü, Nur Z, Ak K. ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ. iuvfd. November 2012;33(1):11-21. doi:10.16988/iuvfd.84406
Chicago Bacınoğlu, Süleyman, Ümit Cirit, Zakeriya Nur, and Kemal Ak. “ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 33, no. 1 (November 2012): 11-21. https://doi.org/10.16988/iuvfd.84406.
EndNote Bacınoğlu S, Cirit Ü, Nur Z, Ak K (November 1, 2012) ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 33 1 11–21.
IEEE S. Bacınoğlu, Ü. Cirit, Z. Nur, and K. Ak, “ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ”, iuvfd, vol. 33, no. 1, pp. 11–21, 2012, doi: 10.16988/iuvfd.84406.
ISNAD Bacınoğlu, Süleyman et al. “ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 33/1 (November 2012), 11-21. https://doi.org/10.16988/iuvfd.84406.
JAMA Bacınoğlu S, Cirit Ü, Nur Z, Ak K. ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ. iuvfd. 2012;33:11–21.
MLA Bacınoğlu, Süleyman et al. “ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 33, no. 1, 2012, pp. 11-21, doi:10.16988/iuvfd.84406.
Vancouver Bacınoğlu S, Cirit Ü, Nur Z, Ak K. ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ. iuvfd. 2012;33(1):11-2.