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Brusella melitensis Şuşlarının İlk İzolasyonu İçin Farrell Besiyerine Eritritol İlavesinin Değerlendirilmesi

Year 2019, Volume: 12 Issue: 1, 75 - 81, 31.03.2019
https://doi.org/10.30607/kvj.494891

Abstract

Bu çalışmanın amacı, B.melitensis şuşlarının ilk izolasyonu için selektif Farrel
besiyerine (FB) eritritol ilavesini değerlendirmektir. Selektif besiyerleri ve
özellikle FB, Brusella (B.)
etkenlerinin izolasyonunda yaygın olarak kullanılmaktadır. Bruselloz teşhisinde
altın standart bakteri izolasyonudur. Selektif besiyerlerinin izolasyon
başarısı içeriklerine bağlıdır. Özellikle kontamine örneklerde izolasyon yüzdesi
düşebilmektedir. Bu bakımdan Brusella
türlerinin besiyerlerinde üremelerini provoke edecek eritritol gibi
bileşenlerin selektif besiyerlerine ilavesi izolasyona fayda sağlayabilir.
Çalışmada Brusella melitensis
şuşlarından kaynaklı 14 küçük ruminant atık vakasına ait iç organ numuneleri
kullanılmıştır. Örneklerden hazırlanan suspansiyonlar eş zamanlı olarak FB ve
eritritol ilaveli Farrel besiyerine (F+ER) inokule edilmiştir. İnkubasyon
boyunca besiyerleri gözlemlenmiş ve besiyerlerinin gösterdiği izolasyon ve
inhibisyon performansları takip edilmiştir. Özellikle eritriol ilavesi, koloni
gelişim hızı ve koloni çapı büyüklüğü sağlayarak daha erken izolasyon imkanı
verebilmesi açısından karşılaştırılmıştır. 14 numune arasında 12’si için F+ER
besiyerinde koloni gelişim hızı veya koloni çapı büyüklüğüne göre izolasyon
için avantaj elde edilmiştir. Sonuçlar selektif besiyerine eritritol ilavesinin
tavsiye edilmesini özellikle kontaminant inhibisyonun yetersiz kaldığı
numuneler için desteklemektedir. Bu nedenle çalışmada olduğu gibi eritritolun,
FB gibi selektif besiyeri formulasyonlarına dahil edilmesi ile yüksek izolasyon
başarısının elde edilmesi mümkün görünmektedir.  

References

  • Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the brucellosis laboratory. Institut National de la Recherche Agromique-INRA, Paris, France. 1988.
  • Anderson JD, Smith H. The Metabolism of Erythritol by Brucella abortus. J Gen Microbiol. 1965; 38:109-124.
  • Corbel, MJ. Microbiological Aspects, In: Madkour's Brucellosis. Ed; Madkour MM. Springer, Berlin. 2001: pp. 51-64.
  • Corbel MJ. Brucellosis In Human And Animals. WHO Press, Geneva, Switzerland. 2006.
  • De Miguel MM, Marin CM, Munoz PM, Dieste L, Grillo MJ, Blasco JM. Development of a selective culture medium for primary isolation of the main Brucella species. J Clin Microbiol. 2011; 49(4):1458-1463.
  • Doganay M, Aygen B. Human Brucellosis: an overview. Int J Infect. Dis. 2003; 7:173-182.
  • Farrell ID. The development of a new selective medium for the isolation of Brucellaabortus from contaminated sources. Res Vet Sci. 1974; 16:280-286.
  • Garcia-Lobo JM, Sangari Garcia JF. Erythritol metobolism and virulence in Brucella. In: Brucella, Molecular and Celular Biology, Ed; Lopez-Goni I, Moriyon I, Taylor& Francis, Spain. 2005; pp. 223-236.
  • Garin-Bastuji B, Blasco JM, Marin C, Albert D. The diagnosis of brucellosis in sheep and goats, old and new tools. Small Ruminant Res. 2006; 62:63-70.
  • Godfroid J, Cloeckaert A, Liautard JP, Kohler S, Fretin D, Walravens K, Garin-Bastuji B, Letesson JJ. From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brusellosis has continously been a re-emerging zonosis. Vet. Res. 2005; 36:313–326.
  • Her M, Choa DH, Kang SI.The development of a selective medium for the Brucella abortus strains and its comparison with the currently recommended and used medium. Diagn Microbiol Infect Dis. 2010; 67:15–21.
  • Hornsby RL, Jensen AE, Olsen SC, Thoen CC. Selective media for isolation of Brucella abortus strain RB51. Vet Microbiol. 2000; 73:51-60.
  • Hunter D, Kearns M. A comparison of three selective media for the isolation of Brucella abortus from contaminated material. Br Vet J. 1977; 133:486-489.
  • International Organization for Standardization. ISO/TS 11133-1.Microbiology of Food and Animal Feeding Stuff–Guidelines on Preparation and Production of Culture Media. Geneva, Switzerland: ISO, 2009.
  • Jones LM, Dubray G, Marly J. Comparison of methods of diagnosis of Brucella ovis infection of rams. Ann Rech Vet 1975; 6:11-22.
  • Karagul MS. Brusella türlerinin izolasyonunda kullanilan dört farkli selektif besiyerinin izolasyon sensitivitelerinin karşılaştırılmasiı ve referans suşların saptanabilirliğinin belirlenmesi. Doktora Tezi, İstanbul Üniversitesi Sağlık Bilimleri Enstitüsü, İstanbul, 2016.
  • Karagul MS, Ikiz S. Comparison of the isolation and inhibition abilities of selective media used for Brucella spp. Isolation. Turk J Vet Anim Sci 2017; 41:781-786.
  • Karagul MS, Ikiz S. The evaluation of Brucella spp. isolation rates in ruminant abortion cases by using different selective media Mac Vet Rev 2018; 41(2):177-186.
  • Keppie J, Williams A, Witt K, Smith H. The role of erythritol in tissue localization of the Brucellae. Brit. J. Exp. Pathol. 1965; 46:104–108. Krstevski K, Naletoski I, Mitrov D, Mrenoshki S, Cvetkovikj I, Janevski A, Dodovski A, Djadjovski I. Application of fluorescence based molecular assays for improved detection and typing of Brucella strains in clinical samples. Mac Vet Rev. 2015; 38(2):223-232.
  • Madkour MM. Endocrinal Brucellosis, In: Madkour's Brucellosis. Berlin: Ed; Madkour, M.M. Springer, Berlin. 2001: pp 179-186.
  • Marin CM, Alabart JL, Blasco JM. Effect of antibioctics contained in two Brucella selective media on growth of Brucella abortus, B.melitensis, and B.ovis. J Clin Microbiol. 1996a; 34(2):426-428.
  • Marin CM, Jimenez De Bagues MP, Barberan M, Blasco JM. Comparison of two selective media for the isolation of Brucella melitensis from naturally infected sheep and goats. Vet Res. 1996b; 138:409-411.
  • Nardi Junior G, Megid J, Vicente AF, Listoni FJ, Monteiro FM, Lara GHB, Motta RG, Chacur MGM, Ribeiro MG. Comparison of Brucella agar, CITA and Farrell media for selective isolation of Brucella abortus from semen of bovine bulls. Afr J Microbiol Res. 2015; 9:617-620.
  • O'Grady D, Byrne W, Kelleher P, O'Callahan H, Kenny K, Heneghan T, Power S, Egan J, Ryan FA. Comparative assessment of culture and serology in the diagnosis. Vet J. 2014; 199:370-375.
  • OIE. Terrestrial Manual Chapter 2.7.2.Caprine and Ovine Brucellosis. 2009.
  • OIE. Terrestrial Manual Chapter 2.4.3. Bovine brucellosis. 2012.
  • Pappas G. The changing Brucella ecology: novel reservoirs, new threats. Int J Antimicrob. 2010; 365:8-11.
  • Pappas G, Panagopoulou P, Chistou L, Akritidis N. Brucella as a biological weapon. Cell Mol Life Sci. 2006; 63:2229-2236.
  • Perez-Sancho M, Garcia-Seco T, Dominguez L, Alvarez, J. Control of Animal Brucellosis-The Most Effective Tool To Prevent Human Brucellosis. In: Updates on Brucellosis. Ed; Baddaur MM, IntechOpen Limited, United Kingdom. 2015; 201-246.
  • Petersen E, Rajashekara G, Sanakkayala N, Eskra L, Harms J, Splitter G. Erythritol triggers expression of virulence traits in Brucella melitensis. Microbes Infect. 2013; 15(0):440–449.
  • Poester FP, Samartino LE, Santos RL. Pathogenesis and pathology of Brucellosis in livestock. Revue scientifique et technique (International Office of Epizootics). 2013; 32(1):105-115.
  • Seleem MN, Boyle SM, Sriranganathan N. Brucella: A pathogen without classic virulence genes. Vet. Microbiol. 2008; 129:1-14.
  • Seleem MN, Boyle SM, Sriranganathan N. Brucellosis:A re-emerging zoonosis. Vet Microbiol. 2010; 140(3-4):392-398.
  • Songer, J.G., K.W. Post. Brusella Cinsi. İçinde Veteriner Hekimlik Mikrobiyolojisi-Hayvan Hastalığı Etkeni Olan Bakteriler ve Mantarlar, Ed; O. Ang, Y. Ozgur, Nobel Matbaacılık, İstanbul, Türkiye. 2012; pp. 200-207.
  • Sperry JF, Robertson DC. Inhibition of Growth by Erythritol Catabolism in Brucella abortus. J Bacteriol. 1975; 124(1):391-397.
  • Stack JA, Harrıson M, Perrett LL. Evaluation of a selective medium for Brucella isolation using natamycin. J Appl Microbiol. 2002; 92:724–728.
  • Vicente AF, Antunes JM, Lara GH, Mioni MSR, Allendorf SD, Peres MG, Appolinario CM, Listoni FJP, Ribeiro MG, Megid J. Evaluation of three formulations of culture media for isolation of Brucella spp. regarding their ability to inhibit the growth of contaminating organisms. Biomed. Res. Int. 2014; 702072.
  • WHO. Laboratory safety manual, Third edition. Geneva. 2004.
  • Yumuk Z, O'Callaghan D. Brucellosis in Turkey-an overview. Int J Infect Dis. 2012; 16:228-235.

Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains

Year 2019, Volume: 12 Issue: 1, 75 - 81, 31.03.2019
https://doi.org/10.30607/kvj.494891

Abstract

This study aims to evaluate the addition of erythritol
to Farrell medium (FM) for the primary isolation of B.melitensis strains. Selective media, particularly FM, is commonly
used for Brucella isolation.
Isolation is the gold standard for the diagnosis of brucellosis. The isolation
success of selective media depends on their ingredients. Isolation rate may
decrease particularly in contaminated samples; therefore, the addition of
components like erythritol that augments the growth of Brucella species on media can support isolation. In this study,
organ samples associated with 14 small ruminant abortion cases caused by B.melitensis strains were utilized.
Organ suspensions were inoculated to FM and Farrel medium with erythritol
(F+ER) simultaneously. The media’s isolation and inhibition performance was
observed during incubation. The addition of erythritol was evaluated based on
whether it provides earlier isolation with the help of increasing the colony
growth rate and colony size. According to the findings, F+ER is more
advantageous for 12 samples among 14 through isolation as it enables faster
colony growth and larger colonies. The results confirm the recommendation of
adding erythritol particularly in the samples with inadequate contaminant
inhibition. Therefore, high isolation performance can be achieved through
erythritol addition to the selective media like FM.

References

  • Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the brucellosis laboratory. Institut National de la Recherche Agromique-INRA, Paris, France. 1988.
  • Anderson JD, Smith H. The Metabolism of Erythritol by Brucella abortus. J Gen Microbiol. 1965; 38:109-124.
  • Corbel, MJ. Microbiological Aspects, In: Madkour's Brucellosis. Ed; Madkour MM. Springer, Berlin. 2001: pp. 51-64.
  • Corbel MJ. Brucellosis In Human And Animals. WHO Press, Geneva, Switzerland. 2006.
  • De Miguel MM, Marin CM, Munoz PM, Dieste L, Grillo MJ, Blasco JM. Development of a selective culture medium for primary isolation of the main Brucella species. J Clin Microbiol. 2011; 49(4):1458-1463.
  • Doganay M, Aygen B. Human Brucellosis: an overview. Int J Infect. Dis. 2003; 7:173-182.
  • Farrell ID. The development of a new selective medium for the isolation of Brucellaabortus from contaminated sources. Res Vet Sci. 1974; 16:280-286.
  • Garcia-Lobo JM, Sangari Garcia JF. Erythritol metobolism and virulence in Brucella. In: Brucella, Molecular and Celular Biology, Ed; Lopez-Goni I, Moriyon I, Taylor& Francis, Spain. 2005; pp. 223-236.
  • Garin-Bastuji B, Blasco JM, Marin C, Albert D. The diagnosis of brucellosis in sheep and goats, old and new tools. Small Ruminant Res. 2006; 62:63-70.
  • Godfroid J, Cloeckaert A, Liautard JP, Kohler S, Fretin D, Walravens K, Garin-Bastuji B, Letesson JJ. From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brusellosis has continously been a re-emerging zonosis. Vet. Res. 2005; 36:313–326.
  • Her M, Choa DH, Kang SI.The development of a selective medium for the Brucella abortus strains and its comparison with the currently recommended and used medium. Diagn Microbiol Infect Dis. 2010; 67:15–21.
  • Hornsby RL, Jensen AE, Olsen SC, Thoen CC. Selective media for isolation of Brucella abortus strain RB51. Vet Microbiol. 2000; 73:51-60.
  • Hunter D, Kearns M. A comparison of three selective media for the isolation of Brucella abortus from contaminated material. Br Vet J. 1977; 133:486-489.
  • International Organization for Standardization. ISO/TS 11133-1.Microbiology of Food and Animal Feeding Stuff–Guidelines on Preparation and Production of Culture Media. Geneva, Switzerland: ISO, 2009.
  • Jones LM, Dubray G, Marly J. Comparison of methods of diagnosis of Brucella ovis infection of rams. Ann Rech Vet 1975; 6:11-22.
  • Karagul MS. Brusella türlerinin izolasyonunda kullanilan dört farkli selektif besiyerinin izolasyon sensitivitelerinin karşılaştırılmasiı ve referans suşların saptanabilirliğinin belirlenmesi. Doktora Tezi, İstanbul Üniversitesi Sağlık Bilimleri Enstitüsü, İstanbul, 2016.
  • Karagul MS, Ikiz S. Comparison of the isolation and inhibition abilities of selective media used for Brucella spp. Isolation. Turk J Vet Anim Sci 2017; 41:781-786.
  • Karagul MS, Ikiz S. The evaluation of Brucella spp. isolation rates in ruminant abortion cases by using different selective media Mac Vet Rev 2018; 41(2):177-186.
  • Keppie J, Williams A, Witt K, Smith H. The role of erythritol in tissue localization of the Brucellae. Brit. J. Exp. Pathol. 1965; 46:104–108. Krstevski K, Naletoski I, Mitrov D, Mrenoshki S, Cvetkovikj I, Janevski A, Dodovski A, Djadjovski I. Application of fluorescence based molecular assays for improved detection and typing of Brucella strains in clinical samples. Mac Vet Rev. 2015; 38(2):223-232.
  • Madkour MM. Endocrinal Brucellosis, In: Madkour's Brucellosis. Berlin: Ed; Madkour, M.M. Springer, Berlin. 2001: pp 179-186.
  • Marin CM, Alabart JL, Blasco JM. Effect of antibioctics contained in two Brucella selective media on growth of Brucella abortus, B.melitensis, and B.ovis. J Clin Microbiol. 1996a; 34(2):426-428.
  • Marin CM, Jimenez De Bagues MP, Barberan M, Blasco JM. Comparison of two selective media for the isolation of Brucella melitensis from naturally infected sheep and goats. Vet Res. 1996b; 138:409-411.
  • Nardi Junior G, Megid J, Vicente AF, Listoni FJ, Monteiro FM, Lara GHB, Motta RG, Chacur MGM, Ribeiro MG. Comparison of Brucella agar, CITA and Farrell media for selective isolation of Brucella abortus from semen of bovine bulls. Afr J Microbiol Res. 2015; 9:617-620.
  • O'Grady D, Byrne W, Kelleher P, O'Callahan H, Kenny K, Heneghan T, Power S, Egan J, Ryan FA. Comparative assessment of culture and serology in the diagnosis. Vet J. 2014; 199:370-375.
  • OIE. Terrestrial Manual Chapter 2.7.2.Caprine and Ovine Brucellosis. 2009.
  • OIE. Terrestrial Manual Chapter 2.4.3. Bovine brucellosis. 2012.
  • Pappas G. The changing Brucella ecology: novel reservoirs, new threats. Int J Antimicrob. 2010; 365:8-11.
  • Pappas G, Panagopoulou P, Chistou L, Akritidis N. Brucella as a biological weapon. Cell Mol Life Sci. 2006; 63:2229-2236.
  • Perez-Sancho M, Garcia-Seco T, Dominguez L, Alvarez, J. Control of Animal Brucellosis-The Most Effective Tool To Prevent Human Brucellosis. In: Updates on Brucellosis. Ed; Baddaur MM, IntechOpen Limited, United Kingdom. 2015; 201-246.
  • Petersen E, Rajashekara G, Sanakkayala N, Eskra L, Harms J, Splitter G. Erythritol triggers expression of virulence traits in Brucella melitensis. Microbes Infect. 2013; 15(0):440–449.
  • Poester FP, Samartino LE, Santos RL. Pathogenesis and pathology of Brucellosis in livestock. Revue scientifique et technique (International Office of Epizootics). 2013; 32(1):105-115.
  • Seleem MN, Boyle SM, Sriranganathan N. Brucella: A pathogen without classic virulence genes. Vet. Microbiol. 2008; 129:1-14.
  • Seleem MN, Boyle SM, Sriranganathan N. Brucellosis:A re-emerging zoonosis. Vet Microbiol. 2010; 140(3-4):392-398.
  • Songer, J.G., K.W. Post. Brusella Cinsi. İçinde Veteriner Hekimlik Mikrobiyolojisi-Hayvan Hastalığı Etkeni Olan Bakteriler ve Mantarlar, Ed; O. Ang, Y. Ozgur, Nobel Matbaacılık, İstanbul, Türkiye. 2012; pp. 200-207.
  • Sperry JF, Robertson DC. Inhibition of Growth by Erythritol Catabolism in Brucella abortus. J Bacteriol. 1975; 124(1):391-397.
  • Stack JA, Harrıson M, Perrett LL. Evaluation of a selective medium for Brucella isolation using natamycin. J Appl Microbiol. 2002; 92:724–728.
  • Vicente AF, Antunes JM, Lara GH, Mioni MSR, Allendorf SD, Peres MG, Appolinario CM, Listoni FJP, Ribeiro MG, Megid J. Evaluation of three formulations of culture media for isolation of Brucella spp. regarding their ability to inhibit the growth of contaminating organisms. Biomed. Res. Int. 2014; 702072.
  • WHO. Laboratory safety manual, Third edition. Geneva. 2004.
  • Yumuk Z, O'Callaghan D. Brucellosis in Turkey-an overview. Int J Infect Dis. 2012; 16:228-235.
There are 39 citations in total.

Details

Primary Language English
Journal Section RESEARCH ARTICLE
Authors

Mustafa Sencer Karagül 0000-0001-7215-5229

Publication Date March 31, 2019
Acceptance Date January 16, 2019
Published in Issue Year 2019 Volume: 12 Issue: 1

Cite

APA Karagül, M. S. (2019). Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains. Kocatepe Veterinary Journal, 12(1), 75-81. https://doi.org/10.30607/kvj.494891
AMA Karagül MS. Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains. kvj. March 2019;12(1):75-81. doi:10.30607/kvj.494891
Chicago Karagül, Mustafa Sencer. “Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella Melitensis Strains”. Kocatepe Veterinary Journal 12, no. 1 (March 2019): 75-81. https://doi.org/10.30607/kvj.494891.
EndNote Karagül MS (March 1, 2019) Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains. Kocatepe Veterinary Journal 12 1 75–81.
IEEE M. S. Karagül, “Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains”, kvj, vol. 12, no. 1, pp. 75–81, 2019, doi: 10.30607/kvj.494891.
ISNAD Karagül, Mustafa Sencer. “Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella Melitensis Strains”. Kocatepe Veterinary Journal 12/1 (March 2019), 75-81. https://doi.org/10.30607/kvj.494891.
JAMA Karagül MS. Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains. kvj. 2019;12:75–81.
MLA Karagül, Mustafa Sencer. “Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella Melitensis Strains”. Kocatepe Veterinary Journal, vol. 12, no. 1, 2019, pp. 75-81, doi:10.30607/kvj.494891.
Vancouver Karagül MS. Evaluation of Adding Erythritol to Farrell Medium for Primary Isolation of the Brucella melitensis Strains. kvj. 2019;12(1):75-81.

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