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Determination of Methicillin Resistance in Coagulase Negative Staphylococci Isolates Obtained from Dogs

Year 2022, Volume: 15 Issue: 4, 368 - 373, 31.12.2022
https://doi.org/10.30607/kvj.1113649

Abstract

Although coagulase negative staphylococci are opportunistic pathogens, they are also isolated from various disease cases in dogs. Methicillin resistant staphylococci also show resistance to other β-lactam group antibiotics, which limits treatment options. In this study, determination of methicillin resistance in coagulase negative Staphylococcus spp. isolates isolated from various samples taken from healthy and sick dogs was aimed. Agar dilution method was used for the determination of methicillin resistance in the isolates. Eighty nine (20.74%) strains were isolated from the total 429 samples by conventional bacteriological methods. Methicillin resistance was found in 19.10% of the isolates. The mecA gene was determined in 11 (64.70%) of the methicillin resistant isolates by PCR. In conclusion, in this study, it was concluded that methicillin resistance should be considered in the treatment of infections in dogs that caused by coagulase negative Staphylococcus spp. isolates, known as opportunistic pathogens.

References

  • Abdel-Moein KA, Zaher HM. The nasal carriage of coagulase-negative staphylococci among animals and its public health implication. Vector Borne Zoonotic Dis. 2020; 20(12):897-902.
  • Aslantaş Ö, Türkyılmaz S, Yılmaz MA, Yılmaz EŞ. Prevalence of methicillin-resistant staphylococci in dogs. Kafkas Univ Vet Fak Derg. 2013; 19(1):37-42.
  • Ba X, Harrison EM, Edwards GF, Holden MT, Larsen AR, Petersen A, Holmes MA. Novel mutations in penicillin-binding protein genes in clinical Staphylococcus aureus isolates that are methicillin resistant on susceptibility testing, but lack the mec gene. J Antimicrob Chemother. 2013; 69(3):594-597.
  • Bierowiec K, Płoneczka-Janeczko K, Rypuła K. Is the colonisation of Staphylococcus aureus in pets associated with their close contact with owners? PLoS One. 2016; 11(5):e0156052.
  • Carter GR. Diagnostic Procedures in Veterinary Bacteriology and Mycology, 4th Ed., Thomas, USA. 1984.
  • Chah KF, Gómez-Sanz E, Nwanta JA, Asadu B, Agbo IC, Lozano C, Zarazaga M, Torres C. Methicillin-resistant coagulase-negative staphylococci from healthy dogs in Nsukka, Nigeria. Braz J Microbiol. 2014; 45(1):215-220.
  • CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, 4th Ed., CLSI supplement VET08, Wayne, Pennsylvania. 2018.
  • Dantes R, Mu Y, Belflower R, Aragon D, Dumyati G, Harrison LH, Ray SM. National burden of invasive methicillin-resistant Staphylococcus aureus infections, United States, 2011. JAMA Intern Med. 2013; 173(21):1970-1978.
  • De Lencastre H, Wu SW, Pinho MG, Ludovice AM, Filipe S, Gardete S, Tomasz, A. Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin. Microb Drug Resist. 1999; 5(3):163-175.
  • Elnageh HR, Hiblu MA, Abbassi MS, Abouzeed YM, Ahmed MO. Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs. Open Vet J. 2020; 10(4):452-456.
  • Gandolfi-Decristophoris P, Regula G, Petrini O, Zinsstag J, Schelling E. Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs. J Vet Sci. 2013; 14(4):449-456.
  • Hariharan H, Sylvester EB, Matthew V. Clinical isolates of bacteria from domestic cats in Grenada, and their antimicrobial susceptibility. WIVJ. 2009; 9:14-16.
  • Khanal M, Joshi PR, Paudel S, Acharya M, Rijal KR, Ghimire P, Banjara MR. Methicillin-resistant coagulase negative staphylococci and their antibiotic susceptibility pattern from healthy dogs and their owners from Kathmandu Valley. Trop Med Infect Dis. 2021; 6:194.
  • Kloos WE, Bannerman T. Update on clinical significance of coagulase-negative Staphylococci. Clin Microbiol Rev. 1994; 7(1):117-140. Köck R, Becker K, Cookson B, van Gemert-Pijnen JE, Harbarth S, Kluytmans JAJW, Tacconelli E. Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Eurosurveillance. 2010; 15(41):19688.
  • Lim D, Strynadka NC. Structural basis for the β lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus. Nat Struct Biol. 2002; 9(11):870-876.
  • Litster A, Moss SM, Honnery M, Rees B, Trott DJ. Prevalence of bacterial species in cats with clinical signs of lower urinary tract disease: recognition of Staphylococcus felis as a possible feline urinary tract pathogen. Vet Microbiol. 2007; 121:182-188.
  • Misic AM, Davis MF, Tyldsley AS, Hodkinson BP, Tolomeo P, Hu B, Nachamkin I, Lautenbach E, Morris DO, Grice EA. The shared microbiota of humans and companion animals as evaluated from Staphylococcus carriage sites. Microbiome. 2015; 3:2.
  • Nemeghaire S, Argudı´n MA, Feßler AT, Hauschild T, Schwarz S, Butaye P. The ecological importance of the Staphylococcus sciuri species group as a reservoir for resistance and virulence genes. Vet Microbiol. 2014; 171(3):342-356.
  • Papini R, Ebani VV, Cerri D, Guidi G. Survey on bacterial isolates from dogs with urinary tract infections and their in vitro sensitivity. Rev Med Vet. 2006; 157(1):35-45.
  • Quinn PJ, Markey BK, Leonard FC, FitzPatrick ES, Fanning S, Hartigan PJ. Staphylococcus species, In: Veterinary Microbiology and Microbial Disease, 2nd Ed., John Wiley & Sons Ltd., UK. 2011. pp. 179-187.
  • Samanta I, Bandyopadhyay S. Staphylococcus, In: Antimicrobial Resistance in Agriculture, Academic Press, London, UK. 2020. pp. 195-215.
  • Siugzdaite J, Gabinaitiene A. Methicillin-resistant coagulase-negative staphylococci in healthy dogs. Vet Med. 2017; 62(09):479-487.
  • Suepaul S, Georges K, Unakal C, Boyen F, Sookhoo J, Ashraph K, Yusuf A, Butaye P. Determination of the frequency, species distribution and antimicrobial resistance of staphylococci isolated from dogs and their owners in Trinidad. PLoS One. 2021; 16(7):e0254048.
  • Suter A, Voelter K, Hartnack S, Spiess BM, Pot SA. Septic keratitis in dogs, cats, and horses in Switzerland: associated bacteria and antibiotic susceptibility. Vet Ophthalmol. 2017; 104:353-360.
  • Teixeira IM, de Oliveira Ferreira E, de Araújo Penna B. Dogs as reservoir of methicillin resistant coagulase negative staphylococci strains–A possible neglected risk. Microb Pathog. 2019; 135:103616.
  • Tsubakishita S, Kuwahara-Arai K, Sasaki T, Hiramatsu K. Origin and molecular evolution of the determinant of methicillin resistance in staphylococci. Antimicrob Agents and Chemother. 2010; 54(10):4352-4359.
  • Yiğit N, Aktaş AE, Doğruman Al F. Klinik örneklerden izole edilen stafilokokların metisilin, fusidik asit ve mupirosin direnci. THDBD. 2008; 65(1):17-23.
  • Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel Multiplex PCR assay for characterization and concomitant subtyping of Staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005; 43(10):5026-5033.

Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması

Year 2022, Volume: 15 Issue: 4, 368 - 373, 31.12.2022
https://doi.org/10.30607/kvj.1113649

Abstract

Koagulaz negatif stafilokoklar, fırsatçı patojen olmalarının yanı sıra köpeklerde çeşitli hastalık olgularından da izole edilmektedir. Metisilin dirençli stafilokoklar diğer β-laktam grubu antibiyotiklere karşı da direnç göstermekte ve bu durum tedavi seçeneklerini kısıtlamaktadır. Bu çalışmada, sağlıklı görünen ve hasta olduğu belirlenen köpeklerden alınan çeşitli örneklerden izole edilen koagulaz negatif Staphylococcus spp. izolatlarında metisilin direncinin belirlenmesi amaçlandı. İzolatlarda metisilin direnci agar dilüsyon yöntemiyle belirlendi. Toplam 429 örnekten konvansiyonel bakteriyolojik yöntemlerle 89 (%20.74) adet koagulaz negatif Staphylococcus spp. izole edildi. İzolatların %19.10’u metisiline dirençli bulundu. Metisilin dirençli izolatların 11 (%64.70)’inde PCR ile mecA geni tespit edildi. Sonuç olarak, bu çalışmada fırsatçı patojenler olarak bilinen koagulaz negatif Staphylococcus spp. izolatlarının köpeklerde neden olabileceği enfeksiyonların tedavisinde metisilin direncine dikkat edilmesi gerektiği kanaatine varıldı.

References

  • Abdel-Moein KA, Zaher HM. The nasal carriage of coagulase-negative staphylococci among animals and its public health implication. Vector Borne Zoonotic Dis. 2020; 20(12):897-902.
  • Aslantaş Ö, Türkyılmaz S, Yılmaz MA, Yılmaz EŞ. Prevalence of methicillin-resistant staphylococci in dogs. Kafkas Univ Vet Fak Derg. 2013; 19(1):37-42.
  • Ba X, Harrison EM, Edwards GF, Holden MT, Larsen AR, Petersen A, Holmes MA. Novel mutations in penicillin-binding protein genes in clinical Staphylococcus aureus isolates that are methicillin resistant on susceptibility testing, but lack the mec gene. J Antimicrob Chemother. 2013; 69(3):594-597.
  • Bierowiec K, Płoneczka-Janeczko K, Rypuła K. Is the colonisation of Staphylococcus aureus in pets associated with their close contact with owners? PLoS One. 2016; 11(5):e0156052.
  • Carter GR. Diagnostic Procedures in Veterinary Bacteriology and Mycology, 4th Ed., Thomas, USA. 1984.
  • Chah KF, Gómez-Sanz E, Nwanta JA, Asadu B, Agbo IC, Lozano C, Zarazaga M, Torres C. Methicillin-resistant coagulase-negative staphylococci from healthy dogs in Nsukka, Nigeria. Braz J Microbiol. 2014; 45(1):215-220.
  • CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, 4th Ed., CLSI supplement VET08, Wayne, Pennsylvania. 2018.
  • Dantes R, Mu Y, Belflower R, Aragon D, Dumyati G, Harrison LH, Ray SM. National burden of invasive methicillin-resistant Staphylococcus aureus infections, United States, 2011. JAMA Intern Med. 2013; 173(21):1970-1978.
  • De Lencastre H, Wu SW, Pinho MG, Ludovice AM, Filipe S, Gardete S, Tomasz, A. Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin. Microb Drug Resist. 1999; 5(3):163-175.
  • Elnageh HR, Hiblu MA, Abbassi MS, Abouzeed YM, Ahmed MO. Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs. Open Vet J. 2020; 10(4):452-456.
  • Gandolfi-Decristophoris P, Regula G, Petrini O, Zinsstag J, Schelling E. Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs. J Vet Sci. 2013; 14(4):449-456.
  • Hariharan H, Sylvester EB, Matthew V. Clinical isolates of bacteria from domestic cats in Grenada, and their antimicrobial susceptibility. WIVJ. 2009; 9:14-16.
  • Khanal M, Joshi PR, Paudel S, Acharya M, Rijal KR, Ghimire P, Banjara MR. Methicillin-resistant coagulase negative staphylococci and their antibiotic susceptibility pattern from healthy dogs and their owners from Kathmandu Valley. Trop Med Infect Dis. 2021; 6:194.
  • Kloos WE, Bannerman T. Update on clinical significance of coagulase-negative Staphylococci. Clin Microbiol Rev. 1994; 7(1):117-140. Köck R, Becker K, Cookson B, van Gemert-Pijnen JE, Harbarth S, Kluytmans JAJW, Tacconelli E. Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Eurosurveillance. 2010; 15(41):19688.
  • Lim D, Strynadka NC. Structural basis for the β lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus. Nat Struct Biol. 2002; 9(11):870-876.
  • Litster A, Moss SM, Honnery M, Rees B, Trott DJ. Prevalence of bacterial species in cats with clinical signs of lower urinary tract disease: recognition of Staphylococcus felis as a possible feline urinary tract pathogen. Vet Microbiol. 2007; 121:182-188.
  • Misic AM, Davis MF, Tyldsley AS, Hodkinson BP, Tolomeo P, Hu B, Nachamkin I, Lautenbach E, Morris DO, Grice EA. The shared microbiota of humans and companion animals as evaluated from Staphylococcus carriage sites. Microbiome. 2015; 3:2.
  • Nemeghaire S, Argudı´n MA, Feßler AT, Hauschild T, Schwarz S, Butaye P. The ecological importance of the Staphylococcus sciuri species group as a reservoir for resistance and virulence genes. Vet Microbiol. 2014; 171(3):342-356.
  • Papini R, Ebani VV, Cerri D, Guidi G. Survey on bacterial isolates from dogs with urinary tract infections and their in vitro sensitivity. Rev Med Vet. 2006; 157(1):35-45.
  • Quinn PJ, Markey BK, Leonard FC, FitzPatrick ES, Fanning S, Hartigan PJ. Staphylococcus species, In: Veterinary Microbiology and Microbial Disease, 2nd Ed., John Wiley & Sons Ltd., UK. 2011. pp. 179-187.
  • Samanta I, Bandyopadhyay S. Staphylococcus, In: Antimicrobial Resistance in Agriculture, Academic Press, London, UK. 2020. pp. 195-215.
  • Siugzdaite J, Gabinaitiene A. Methicillin-resistant coagulase-negative staphylococci in healthy dogs. Vet Med. 2017; 62(09):479-487.
  • Suepaul S, Georges K, Unakal C, Boyen F, Sookhoo J, Ashraph K, Yusuf A, Butaye P. Determination of the frequency, species distribution and antimicrobial resistance of staphylococci isolated from dogs and their owners in Trinidad. PLoS One. 2021; 16(7):e0254048.
  • Suter A, Voelter K, Hartnack S, Spiess BM, Pot SA. Septic keratitis in dogs, cats, and horses in Switzerland: associated bacteria and antibiotic susceptibility. Vet Ophthalmol. 2017; 104:353-360.
  • Teixeira IM, de Oliveira Ferreira E, de Araújo Penna B. Dogs as reservoir of methicillin resistant coagulase negative staphylococci strains–A possible neglected risk. Microb Pathog. 2019; 135:103616.
  • Tsubakishita S, Kuwahara-Arai K, Sasaki T, Hiramatsu K. Origin and molecular evolution of the determinant of methicillin resistance in staphylococci. Antimicrob Agents and Chemother. 2010; 54(10):4352-4359.
  • Yiğit N, Aktaş AE, Doğruman Al F. Klinik örneklerden izole edilen stafilokokların metisilin, fusidik asit ve mupirosin direnci. THDBD. 2008; 65(1):17-23.
  • Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel Multiplex PCR assay for characterization and concomitant subtyping of Staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005; 43(10):5026-5033.
There are 28 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Sciences
Journal Section RESEARCH ARTICLE
Authors

Özgül Gülaydın 0000-0001-8376-2008

İsmail Hakkı Ekin 0000-0001-5029-8130

Ziya İlhan 0000-0003-3638-9196

Muazzez Yeşilyurt 0000-0003-4195-6335

Berivan Kaplan 0000-0003-1671-8562

Rabia Çelik 0000-0002-6136-0443

Early Pub Date December 12, 2022
Publication Date December 31, 2022
Acceptance Date October 5, 2022
Published in Issue Year 2022 Volume: 15 Issue: 4

Cite

APA Gülaydın, Ö., Ekin, İ. H., İlhan, Z., Yeşilyurt, M., et al. (2022). Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması. Kocatepe Veterinary Journal, 15(4), 368-373. https://doi.org/10.30607/kvj.1113649
AMA Gülaydın Ö, Ekin İH, İlhan Z, Yeşilyurt M, Kaplan B, Çelik R. Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması. kvj. December 2022;15(4):368-373. doi:10.30607/kvj.1113649
Chicago Gülaydın, Özgül, İsmail Hakkı Ekin, Ziya İlhan, Muazzez Yeşilyurt, Berivan Kaplan, and Rabia Çelik. “Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması”. Kocatepe Veterinary Journal 15, no. 4 (December 2022): 368-73. https://doi.org/10.30607/kvj.1113649.
EndNote Gülaydın Ö, Ekin İH, İlhan Z, Yeşilyurt M, Kaplan B, Çelik R (December 1, 2022) Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması. Kocatepe Veterinary Journal 15 4 368–373.
IEEE Ö. Gülaydın, İ. H. Ekin, Z. İlhan, M. Yeşilyurt, B. Kaplan, and R. Çelik, “Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması”, kvj, vol. 15, no. 4, pp. 368–373, 2022, doi: 10.30607/kvj.1113649.
ISNAD Gülaydın, Özgül et al. “Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması”. Kocatepe Veterinary Journal 15/4 (December 2022), 368-373. https://doi.org/10.30607/kvj.1113649.
JAMA Gülaydın Ö, Ekin İH, İlhan Z, Yeşilyurt M, Kaplan B, Çelik R. Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması. kvj. 2022;15:368–373.
MLA Gülaydın, Özgül et al. “Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması”. Kocatepe Veterinary Journal, vol. 15, no. 4, 2022, pp. 368-73, doi:10.30607/kvj.1113649.
Vancouver Gülaydın Ö, Ekin İH, İlhan Z, Yeşilyurt M, Kaplan B, Çelik R. Köpeklerden Elde Edilen Koagulaz Negatif Stafilokok İzolatlarında Metisilin Direncinin Araştırılması. kvj. 2022;15(4):368-73.

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