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Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids

Year 2022, Volume: 15 Issue: 4, 443 - 449, 31.12.2022

Hasbi Sait Saltık

https://doi.org/10.30607/kvj.1172589

Abstract

Crimean-Congo hemorrhagic fever (CCHF) virus infection is a tick-borne zoonotic disease that is endemic in a large region of the world and is a potentially fatal disease, especially threatening human health. One of the main indicators that the disease is endemic in a region is the presence of antibodies specific to CCHFV in animal populations. Many animal species may carry CCHFV asymptomatically and hence participate to the disease's transmission cycle. Serological studies have shown that equids are important to the survival of CCHFV in nature. Nevertheless, need for more studies on this subject in Türkiye. For this study, blood samples were taken from 11 horses and 86 donkeys of different sex and variable ages in the provinces of Afyonkarahisar and Burdur. The presence of CCHFV-specific antibodies in the blood serum of these animals, which are held by the breeders for a variety of purposes, was studied. The double-antigen ELISA test method, which is a fast and reliable method, was used for the detection of specific antibodies. As a result, a total of 51.54% (50/97) seropositivity was detected. Findings indicate that equidae may play an important role as a reservoir in the epidemiology of CCHFV.

Keywords

Crimean-Congo hemorrhagic fever donkey ELISA equide seroprevalence

Thanks

The author would like to thank Mustafa Üğdül (DVM) and Oğuzhan Kaplan (DVM, PhD candidate) for field sampling assistance.

References

  • Albayrak H, Ozan E, Kurt M. Serosurvey and molecular detection of Crimean-Congo hemorrhagic fever virus (CCHFV) in northern Turkey. Tropical Animal Health and Production. 2012; 44, 1667-1671.
  • Ali A, Khan MA, Zahid H, Yaseen PM, Qayash Khan M, Nawab J, Ur Rehman Z, Ateeq M, Khan S, Ibrahim M. Seasonal dynamics, record of ticks infesting humans, wild and domestic animals and molecular phylogeny of Rhipicephalus microplus in Khyber Pakhtunkhwa Pakistan. Frontiers in physiology. 2019; 10:793.
  • Ali A, Zahid H, Zeb I, Tufail M, Khan S, Haroon M, Tufail M, Bilal M, Hussain M, Alouffi AS, Muñoz‑Leal S, Labruna MB. Risk factors associated with tick infestations on equids in Khyber Pakhtunkhwa, Pakistan, with notes on Rickettsia massiliae detection. Parasites & Vectors. 2021; 14(1):1-12.
  • Bakırcı S. Batı Anadolu Bölgesi sığırlarında görülen kene türleri ve yaygınlığı. Doktora tezi, Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü, Bursa, 2009.
  • Bente DA, Forrester NL, Watts DM, McAuley AJ, Whitehouse CA, Bray M. Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Antiviral Research. 2013; 100(1):159-189.
  • Blanco-Penedo I, Obanda V, Kingori E, Agwanda B, Ahlm C, Lwande OW. Seroepidemiology of Crimean-Congo Hemorrhagic Fever Virus (CCHFV) in cattle across three livestock pastoral regions in Kenya. Dairy. 2021; 2(3):425-434.
  • Ceianu CS, Panculescu-Gatej RI, Coudrier D, Bouloy M. First serologic evidence for the circulation of Crimean-Congo hemorrhagic fever virus in Romania. Vector Borne Zoonotic Dis. 2012; 12:718-721.
  • Elaldı N. Kırım-Kongo hemorajik ateşi epidemiyolojisi. Klimik Derg. 2004; 17:151-155.
  • Eser M. Afyonkarahisar yöresindeki koyun, keçi ve sığırlarda kene (Ixodidae) infestasyonu üzerine araştırmalar. Doktora tezi, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyonkarahisar, 2012.
  • Fazlalipour M, Baniasadi V, Mirghiasi SM, Jalali T, Khakifirouz S, Azad-Manjiri S, Mahmoodi V, Naderi HR, Zarandi R, Salehi-Vaziri M. Crimean-congo hemorrhagic fever due to consumption of raw meat: Case reports from East-North of Iran. Japanese Journal of Infectious Diseases. 2016; 69(3):270-271.
  • Gargili A, Estrada-Peña A, Spengler JR, Lukashev A, Nuttall PA, Bente DA. The role of ticks in the maintenance and transmission of Crimean-Congo hemorrhagic fever virus: A review of published field and laboratory studies. Antiviral Research. 2017; 144:93-119.
  • Hawman DW, Feldmann H. Recent advances in understanding Crimean–Congo hemorrhagic fever virus. F1000Research. 2018; 7.
  • Ibrahim AM, Adam IA, Osman BT, Aradaib IE. Epidemiological survey of Crimean Congo hemorrhagic fever virus in cattle in East Darfur State, Sudan. Ticks and tick-borne diseases. 2015; 6(4):439-444.
  • Kaba T. Geographical distribution of ixodid ticks and tick-borne pathogens of domestic animals in Ethiopia: a systematic review. Parasites & Vectors, 2022; 15(1):1-26.
  • Leblebicioglu H, Ozaras R, Irmak H, Sencan I. Crimean-Congo hemorrhagic fever in Turkey: Current status and future challenges. Antiviral research. 2016; 126:21-34.
  • Lombe BP, Miyamoto H, Saito T, Yoshida R, Manzoor R, Kajihara M, Shimojima M, Fukushi S, Morikawa S, Yoshikawa T, Kurosu T, Saijo M, Tang Q, Masumu J, Hawman D, Feldmann H, Takada A. Purification of Crimean–Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis. Scientific reports. 2021; 11(1):1-11.
  • Lwande OW, Irura Z, Tigoi C, Chepkorir E, Orindi B, Musila I, Venter M, Fischer A, Sang R. Seroprevalence of Crimean Congo hemorrhagic fever virus in Ijara District, Kenya. Vector Borne Zoonotic Dis. 2012; 12:1-6.
  • Mangombi JB, Roqueplo C, Sambou M, Dahmani M, Mediannikov O, Comtet L, Davoust B. Seroprevalence of Crimean-Congo hemorrhagic fever in domesticated animals in Northwestern Senegal. Vector-Borne and Zoonotic Diseases. 2020; 20(10):797-799.
  • Mostafavi E, Pourhossein B, Esmaeili S, Amiri FB, Khakifirouz S, Shah-Hosseini N, Tabatabaei SM. Seroepidemiology and risk factors of Crimean-Congo Hemorrhagic Fever among butchers and slaughterhouse workers in southeastern Iran. International Journal of Infectious Diseases. 2017; 64:85-89.,
  • Öztürk SB, Kırdar S, Ertuğrul MB, Turan C, Türe M. A New Endemic Province of Crimean-Congo Haemorrhagic Fever in Turkey: Aydin/Kirim-Kongo Kanamali Atesi Icin Yeni Bir Endemik Il: Aydin. Klimik Journal. 2017; 30(1), 9-15.
  • Pak TP. Division into epidemiological districts of Crimean haemorrhagic fever (CHF) in the Tadzhik SSR. Zh Mikrobiol Epidemiol Immunobiol. 1972; 12:112-116.
  • Sana M, Javed A, Jamal SB, Junaid M, Faheem M. Development of multivalent vaccine targeting M segment of Crimean Congo Hemorrhagic Fever Virus (CCHFV) using immunoinformatic approaches. Saudi journal of biological sciences. 2022; 29(4):2372-2388.
  • Sas MA, Comtet L, Donnet F, Mertens M, Vatansever Z, Tordo N, Pourquier P, Groschup MH. A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies. Antiviral research. 2018; 151:24-26.
  • Shahid MF, Yaqub T, Ali M, Ul-Rahman A, Bente DA. Prevalence and phylogenetic analysis of Crimean-Congo hemorrhagic fever virus in ticks collected from Punjab province of Pakistan. Acta Tropica. 2021; 218:105892.
  • Sorvillo TE, Rodriguez SE, Hudson P, Carey M, Rodriguez LL, Spiropoulou CF, Bird BH, Spengler JR, Bente DA. Towards a sustainable one health approach to crimean–congo hemorrhagic fever prevention: Focus areas and gaps in knowledge. Trop Med Infect Dis. 2020; 5:113.
  • Spengler JR, Bergeron É, Rollin PE. Seroepidemiological studies of Crimean-Congo hemorrhagic fever virus in domestic and wild animals. PLoS neglected tropical diseases. 2016; 10(1):e0004210.
  • Şimşek Ç, Özkan S, Çulha G, Salnur B, Yılmaz T, Dizbay M. Türkiye’nin İki İlinde Yaşayanların Kırım Kongo Kanamalı Ateşi (KKKA) Hastalığı Konusundaki Bilgi ve Tutumları. Sağlık ve Toplum. 2018; 28(2):38-46.
  • Tuncer P, Yesilbağ K, Alpay G, Dinçer E, Girisgin AO, Aydın L, Uyar Y, Ozkul A. Crimean-Congo hemorrhagic fever infection in domestic animals in Marmara region, Western Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2014; 61(1), 49-53.

Tek tırnaklı hayvanlarda Kırım Kongo Hemorajik Ateşi Virusu'na spesifik antikorların tespiti

Year 2022, Volume: 15 Issue: 4, 443 - 449, 31.12.2022

Hasbi Sait Saltık

https://doi.org/10.30607/kvj.1172589

Abstract

Kırım Kongo kanamalı ateşi (KKKA) virus enfeksiyonu, dünyanın geniş bir bölgesinde endemik olan ve özellikle insan sağlığını tehdit eden, potansiyel olarak ölümcül bir hastalık olan kene kaynaklı zoonotik bir hastalıktır. Hastalığın bir bölgede endemik olduğunun ana göstergelerinden biri, hayvan popülasyonlarında KKKAV'ye özgü antikorların varlığıdır. Birçok hayvan türü KKKAV'yi asemptomatik olarak taşıyabilir ve bu nedenle hastalığın bulaşma döngüsüne katılabilir. Serolojik çalışmalar, doğada KKKAV' nin hayatta kalması için tek tırnaklıların önemli olduğunu göstermiştir. Ancak Türkiye’de bu konuda daha fazla çalışmaya ihtiyaç vardır. Araştırma için Afyonkarahisar ve Burdur illerinde çeşitli cinsiyet ve yaştaki 11 at ve 86 eşekten kan örnekleri alındı. Yetiştiriciler tarafından çeşitli amaçlarla tutulan bu hayvanların kan serumlarında KKKV'ye özgü antikorların varlığı araştırıldı. Spesifik antikorların tespiti için hızlı ve güvenilir bir yöntem olan çift antijenli ELISA test yöntemi kullanıldı. Sonuç olarak toplamda %51.54 (50/97) seropozitiflik tespit edildi. Bulgular, tek tırnaklı hayvanların KKKA'nın epidemiyolojisinde rezervuar olarak önemli bir rol oynayabileceğini göstermektedir.

Keywords

ELISA equide eşek Kırım Kongo Kanamalı Ateşi Virusu seroprevalans

References

  • Albayrak H, Ozan E, Kurt M. Serosurvey and molecular detection of Crimean-Congo hemorrhagic fever virus (CCHFV) in northern Turkey. Tropical Animal Health and Production. 2012; 44, 1667-1671.
  • Ali A, Khan MA, Zahid H, Yaseen PM, Qayash Khan M, Nawab J, Ur Rehman Z, Ateeq M, Khan S, Ibrahim M. Seasonal dynamics, record of ticks infesting humans, wild and domestic animals and molecular phylogeny of Rhipicephalus microplus in Khyber Pakhtunkhwa Pakistan. Frontiers in physiology. 2019; 10:793.
  • Ali A, Zahid H, Zeb I, Tufail M, Khan S, Haroon M, Tufail M, Bilal M, Hussain M, Alouffi AS, Muñoz‑Leal S, Labruna MB. Risk factors associated with tick infestations on equids in Khyber Pakhtunkhwa, Pakistan, with notes on Rickettsia massiliae detection. Parasites & Vectors. 2021; 14(1):1-12.
  • Bakırcı S. Batı Anadolu Bölgesi sığırlarında görülen kene türleri ve yaygınlığı. Doktora tezi, Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü, Bursa, 2009.
  • Bente DA, Forrester NL, Watts DM, McAuley AJ, Whitehouse CA, Bray M. Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Antiviral Research. 2013; 100(1):159-189.
  • Blanco-Penedo I, Obanda V, Kingori E, Agwanda B, Ahlm C, Lwande OW. Seroepidemiology of Crimean-Congo Hemorrhagic Fever Virus (CCHFV) in cattle across three livestock pastoral regions in Kenya. Dairy. 2021; 2(3):425-434.
  • Ceianu CS, Panculescu-Gatej RI, Coudrier D, Bouloy M. First serologic evidence for the circulation of Crimean-Congo hemorrhagic fever virus in Romania. Vector Borne Zoonotic Dis. 2012; 12:718-721.
  • Elaldı N. Kırım-Kongo hemorajik ateşi epidemiyolojisi. Klimik Derg. 2004; 17:151-155.
  • Eser M. Afyonkarahisar yöresindeki koyun, keçi ve sığırlarda kene (Ixodidae) infestasyonu üzerine araştırmalar. Doktora tezi, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyonkarahisar, 2012.
  • Fazlalipour M, Baniasadi V, Mirghiasi SM, Jalali T, Khakifirouz S, Azad-Manjiri S, Mahmoodi V, Naderi HR, Zarandi R, Salehi-Vaziri M. Crimean-congo hemorrhagic fever due to consumption of raw meat: Case reports from East-North of Iran. Japanese Journal of Infectious Diseases. 2016; 69(3):270-271.
  • Gargili A, Estrada-Peña A, Spengler JR, Lukashev A, Nuttall PA, Bente DA. The role of ticks in the maintenance and transmission of Crimean-Congo hemorrhagic fever virus: A review of published field and laboratory studies. Antiviral Research. 2017; 144:93-119.
  • Hawman DW, Feldmann H. Recent advances in understanding Crimean–Congo hemorrhagic fever virus. F1000Research. 2018; 7.
  • Ibrahim AM, Adam IA, Osman BT, Aradaib IE. Epidemiological survey of Crimean Congo hemorrhagic fever virus in cattle in East Darfur State, Sudan. Ticks and tick-borne diseases. 2015; 6(4):439-444.
  • Kaba T. Geographical distribution of ixodid ticks and tick-borne pathogens of domestic animals in Ethiopia: a systematic review. Parasites & Vectors, 2022; 15(1):1-26.
  • Leblebicioglu H, Ozaras R, Irmak H, Sencan I. Crimean-Congo hemorrhagic fever in Turkey: Current status and future challenges. Antiviral research. 2016; 126:21-34.
  • Lombe BP, Miyamoto H, Saito T, Yoshida R, Manzoor R, Kajihara M, Shimojima M, Fukushi S, Morikawa S, Yoshikawa T, Kurosu T, Saijo M, Tang Q, Masumu J, Hawman D, Feldmann H, Takada A. Purification of Crimean–Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis. Scientific reports. 2021; 11(1):1-11.
  • Lwande OW, Irura Z, Tigoi C, Chepkorir E, Orindi B, Musila I, Venter M, Fischer A, Sang R. Seroprevalence of Crimean Congo hemorrhagic fever virus in Ijara District, Kenya. Vector Borne Zoonotic Dis. 2012; 12:1-6.
  • Mangombi JB, Roqueplo C, Sambou M, Dahmani M, Mediannikov O, Comtet L, Davoust B. Seroprevalence of Crimean-Congo hemorrhagic fever in domesticated animals in Northwestern Senegal. Vector-Borne and Zoonotic Diseases. 2020; 20(10):797-799.
  • Mostafavi E, Pourhossein B, Esmaeili S, Amiri FB, Khakifirouz S, Shah-Hosseini N, Tabatabaei SM. Seroepidemiology and risk factors of Crimean-Congo Hemorrhagic Fever among butchers and slaughterhouse workers in southeastern Iran. International Journal of Infectious Diseases. 2017; 64:85-89.,
  • Öztürk SB, Kırdar S, Ertuğrul MB, Turan C, Türe M. A New Endemic Province of Crimean-Congo Haemorrhagic Fever in Turkey: Aydin/Kirim-Kongo Kanamali Atesi Icin Yeni Bir Endemik Il: Aydin. Klimik Journal. 2017; 30(1), 9-15.
  • Pak TP. Division into epidemiological districts of Crimean haemorrhagic fever (CHF) in the Tadzhik SSR. Zh Mikrobiol Epidemiol Immunobiol. 1972; 12:112-116.
  • Sana M, Javed A, Jamal SB, Junaid M, Faheem M. Development of multivalent vaccine targeting M segment of Crimean Congo Hemorrhagic Fever Virus (CCHFV) using immunoinformatic approaches. Saudi journal of biological sciences. 2022; 29(4):2372-2388.
  • Sas MA, Comtet L, Donnet F, Mertens M, Vatansever Z, Tordo N, Pourquier P, Groschup MH. A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies. Antiviral research. 2018; 151:24-26.
  • Shahid MF, Yaqub T, Ali M, Ul-Rahman A, Bente DA. Prevalence and phylogenetic analysis of Crimean-Congo hemorrhagic fever virus in ticks collected from Punjab province of Pakistan. Acta Tropica. 2021; 218:105892.
  • Sorvillo TE, Rodriguez SE, Hudson P, Carey M, Rodriguez LL, Spiropoulou CF, Bird BH, Spengler JR, Bente DA. Towards a sustainable one health approach to crimean–congo hemorrhagic fever prevention: Focus areas and gaps in knowledge. Trop Med Infect Dis. 2020; 5:113.
  • Spengler JR, Bergeron É, Rollin PE. Seroepidemiological studies of Crimean-Congo hemorrhagic fever virus in domestic and wild animals. PLoS neglected tropical diseases. 2016; 10(1):e0004210.
  • Şimşek Ç, Özkan S, Çulha G, Salnur B, Yılmaz T, Dizbay M. Türkiye’nin İki İlinde Yaşayanların Kırım Kongo Kanamalı Ateşi (KKKA) Hastalığı Konusundaki Bilgi ve Tutumları. Sağlık ve Toplum. 2018; 28(2):38-46.
  • Tuncer P, Yesilbağ K, Alpay G, Dinçer E, Girisgin AO, Aydın L, Uyar Y, Ozkul A. Crimean-Congo hemorrhagic fever infection in domestic animals in Marmara region, Western Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2014; 61(1), 49-53.
There are 28 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences
Journal Section RESEARCH ARTICLE
Authors

Hasbi Sait Saltık 0000-0002-3283-7062

Early Pub Date December 12, 2022
Publication Date December 31, 2022
Acceptance Date November 12, 2022
Published in Issue Year 2022 Volume: 15 Issue: 4

Cite

APA Saltık, H. S. (2022). Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids. Kocatepe Veterinary Journal, 15(4), 443-449. https://doi.org/10.30607/kvj.1172589
AMA Saltık HS. Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids. kvj. December 2022;15(4):443-449. doi:10.30607/kvj.1172589
Chicago Saltık, Hasbi Sait. “Crimean Congo Hemorrhagic Fever Virus-Specific Antibody Detection in Equids”. Kocatepe Veterinary Journal 15, no. 4 (December 2022): 443-49. https://doi.org/10.30607/kvj.1172589.
EndNote Saltık HS (December 1, 2022) Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids. Kocatepe Veterinary Journal 15 4 443–449.
IEEE H. S. Saltık, “Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids”, kvj, vol. 15, no. 4, pp. 443–449, 2022, doi: 10.30607/kvj.1172589.
ISNAD Saltık, Hasbi Sait. “Crimean Congo Hemorrhagic Fever Virus-Specific Antibody Detection in Equids”. Kocatepe Veterinary Journal 15/4 (December 2022), 443-449. https://doi.org/10.30607/kvj.1172589.
JAMA Saltık HS. Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids. kvj. 2022;15:443–449.
MLA Saltık, Hasbi Sait. “Crimean Congo Hemorrhagic Fever Virus-Specific Antibody Detection in Equids”. Kocatepe Veterinary Journal, vol. 15, no. 4, 2022, pp. 443-9, doi:10.30607/kvj.1172589.
Vancouver Saltık HS. Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids. kvj. 2022;15(4):443-9.
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