Farklı Barındırma Koşullarındaki Kınalı Kekliklerde (Alectoris chuckar) Bazı Biyokimyasal Parametreler ve Oksidatif Stres Düzeylerinin Araştırılması
Abstract
Oksidatif stres, canlının bedenindeki serbest radikaller ve antioksidanlar arasındaki dengesizlik sonucu oluşur. Bu çalışmada kınalı kekliklerde farklı yetiştirilme koşullarının oksidatif stres düzeyleri üzerine etkileri araştırıldı. Araştırma materyalini Doğa Koruma ve Milli Parklar V. Bölge Müdürlüğüne bağlı Şuhut Keklik Üretme İstasyonunda doğaya salım yapmak üzere yetiştirilen 60 adet, benzer yaş ve ağırlıkta damızlık erkek kınalı keklik oluşturdu. İki gruba ayrılan ve 30 adedi kafes sisteminde 30 adedi de voliyerlere salınarak 3 hafta bakılan kınalı kekliklerden kan alınarak bazı biyokimyasal ve oksidatif stres parametrelerine bakıldı. Araştırma sonucunda biyokimyasal parametrelerden glukoz, trigliserid ve total kolesterol, üre ve kreatinin değerlerinin, Lipid peroksidasyon belirteci olan MDA değerlerinin voliyerde barındırılan kekliklerde kafeste barındırılan kekliklere göre daha düşük düzeyde (p<0.05), glutatyon, SOD ve CAT aktiviteleri ile beta karoten ve A vitamini değerlerinin ise daha yüksek düzeyde olduğu (p<0.05) tespit edildi. Aspartat aminotransferaz, ALT ve ALP aktiviteleri enzim ile total protein düzeylerinin ise her iki barındırma metodundan etkilenmediği (p>0.05) belirlendi. Araştırma sonucunda kafeste barındırılan kekliklerde voliyerdekilere göre böbrek fonksiyonlarını gösteren parametrelerden kreatinin ve üre değerlerinin düşük olduğu, karaciğer enzimlerinin değişmediği ve oksidatif stresin oluştuğu tespit edildi. Sonuç olarak oksidatif stres düzeyi açısından kınalı kekliklerin voliyerlerde barındırılmalarının daha uygun olduğu, kafes sisteminde barındırıldıkları koşullarda ise antioksidanlarla desteklenmelerinin faydalı olacağı kanaatine varıldı.
Supporting Institution
Doğa Koruma ve Milli Parklar V. Bölge Müdürlüğü
Project Number
-
Thanks
Bu projenin gerçekleştirilmesinde emeği geçen başta Veteriner Hekim Ali Gezer olmak üzere Şuhut Keklik Üretim İstasyonu çalışanlarına, Doğa Koruma ve Milli Parklar V. Bölge Müdürlüğü yetkililerine ve projedeki yardımlarından dolayı Veteriner hekimler Talat Ergün ile Cansel Aydın’a teşekkürü bir borç biliriz.
References
- Agarwal, M., Parameswari, R. P., Vasanthi, H. R., & Das, D. K. (2012). Dynamic action of carotenoids in cardioprotection and maintenance of cardiac health. Molecules, 17(4), 4755-4769.
- Alonso-Alvarez, C., Pérez-Rodríguez, L., García, J. T., Vinuela, J., & Mateo, R. (2010). Age and breeding effort as sources of individual variability in oxidative stress markers in a bird species. Physiological and Biochemical Zoology, 83(1), 110-118.
- Beutler, E., Duron, O., Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888.
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- Keskin, E., Çöl, R., Keçeci, T., İpek, H., & Önder, F. (2002). Konya bölgesinde yetiştirilen kaya kekliklerinde (Allectoris gracea) bazı hematolojik parametreler. Veteriner Bilimleri Dergisi. Eurasian Journal of Veterinary Sciences, 18(3-4), 23-27.
- Kırıkçı, K., & Çetin, O. (1999). Keklik yetiştiriciliği. Türk Vet Hek Derg, 11(1-2), 15-18.
- Macit, S., & Akbulut, G. (2015). Diabetes mellitus ve oksidatif stres. Beslenme ve Diyet Dergisi, 43(1), 59-65.
- Özbey, O., & Esen, F. (2007). The effects of breeding systems and stocking density on some blood parameters of rock partridges (Alectoris graeca). Poultry science, 86(2), 420-422.
- Özçelik, M. (1995). Kuşlar Dünyası, Bilim ve Teknik. 328, 66- 73.
- Özek K, Bahtiyarca Y. (2004). Effects of sex and protein and energy levels in the diet on the blood parameters of the chukar partridge (Alectoris chukar). Br Poult Sci, 45(2): 290-293).
- Özhan N, Şimşek Ü.G., Özçelik M. (2016). Comparison of floor and cage housing systems in terms of some welfare assessments in broiler, Ankara Üniv Vet Fak Derg, 63, 317-322.
- Perez-Rodriguez, L., Mougeot, F., Alonso-Alvarez, C., Blas, J., Viñuela, J., & Bortolotti, G. R. (2008). Cell-mediated immune activation rapidly decreases plasma carotenoids but does not affect oxidative stress in red-legged partridges (Alectoris rufa). Journal of Experimental Biology, 211(13), 2155-2161.
- Sinha, A. K. (1972). Colorimetric assay of catalase. Analytical biochemistry, 47(2), 389-394.
- Storz, G., & Imlayt, J. A. (1999). Oxidative stress. Current opinion in microbiology, 2(2), 188-194.
- Sun, Y. I., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 34(3), 497-500.
- Suzuki, J., & Katoh, N. (1990). A simple and cheap methods for measuring serum vitamin A in cattle using only a spectrophotometer. The Japanese Journal of Veterinary Science, 52(6), 1281–1283.
- Şimşek, Ü. G., Erişir, M., Çiftçi, M., & Seven, P. T. (2014). Effects of cage and floor housing systems on fattening performance, oxidative stress and carcass defects in broiler chicken. Kafkas Vet Fak Derg, 20, 727-733.
- Valko M., D. Leibfritz, J. Moncola, M.T.D. Cronin, M. Mazura, and J. Telser. (2007). Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84.
- Winterbourn CC, Hawkins RE, Brian M, Carrell RW.(1975). The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine. 85(2): 337-341.
- Yılmaz B. (2000). Kan “Fizyoloji" 116- 135, Feryal Matbaacılık, Ankara.
Investigation of Some Biochemical Parameters and Oxidative Stress Levels in Chuckar Partridges (Alectoris chuckar) in Different Housing Conditions
Abstract
Oxidative stress occurs as a result of an imbalance between free radicals and antioxidants in the organism's body. In this study, the effects of different growing conditions on oxidative stress levels in chuckar partridge were investigated. The research material was formed by 60 male breeding male chuckar partridges of similar age and weight, bred at the Şuhut Partridge Production Station affiliated to the V. Regional Directorate of Nature Conservation and National Parks. Blood was taken from partridges and some biochemical and oxidative stress parameters were checked. As a result of the research, glucose, triglyceride and total cholesterol, urea and creatinine values from biochemical parameters, MDA values, which are lipid peroxidation markers, were lower in partridges housed in voles than in partridges housed in cages (p<0.05), glutathione, SOD and CAT activities and beta carotene and vitamin A values were found to be higher (p<0.05). It was determined that Aspartate aminotransferase, ALT and ALP activities, enzyme and total protein levels were not affected by both hosting methods (p>0.05). As a result of the research, it was determined that creatinine and urea values, which are parameters showing kidney functions, were lower in the partridges housed in the cage compared to those in the volier, liver enzymes did not change and oxidative stress occurred. As a result, it was concluded that in terms of oxidative stress level, it is more appropriate to keep the chuckar partridges in volies, and it would be beneficial to support them with antioxidants when they are housed in a cage system.
Project Number
-
References
- Agarwal, M., Parameswari, R. P., Vasanthi, H. R., & Das, D. K. (2012). Dynamic action of carotenoids in cardioprotection and maintenance of cardiac health. Molecules, 17(4), 4755-4769.
- Alonso-Alvarez, C., Pérez-Rodríguez, L., García, J. T., Vinuela, J., & Mateo, R. (2010). Age and breeding effort as sources of individual variability in oxidative stress markers in a bird species. Physiological and Biochemical Zoology, 83(1), 110-118.
- Beutler, E., Duron, O., Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888.
- Birben, E., Sahiner, U. M., Sackesen, C., Erzurum, S., & Kalayci, O. (2012). Oxidative stress and antioxidant defense. World allergy organization journal, 5, 9-19.
- Chapman, M. S. (2012, March). Vitamin a: history, current uses, and controversies. In Seminars in cutaneous medicine and surgery (Vol. 31, No. 1, pp. 11-16). WB Saunders.
- Drabkin, D.L., Austin, J.H. (1935). Spectrophotometric studies. II. Preparations fromwashed 112, blood cells; nitric oxide hemoglobin and sulfhemoglobin. J Biol Chem:51–65.
- Draper, H. H., & Hadley, M. (1990). [43] Malondialdehyde determination as index of lipid Peroxidation. In Methods in enzymology (Vol. 186, pp. 421-431). Academic press.
- Durak, M. H., Gökalp, E., & Gürgöze, S. (2016). Kınalı Kekliklerde (Alectoris Chukar) Bazı Hematolojik ve Biyokimyasal Parametreler İle Mineral Madde Düzeyleri Üzerine Cinsiyetin Etkisi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, (1), 22-27.
- Gaweł S, Wardas M, Niedworok E, Wardas P (2004) Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad Lek, 57, 453-455.
- Keskin, E., Çöl, R., Keçeci, T., İpek, H., & Önder, F. (2002). Konya bölgesinde yetiştirilen kaya kekliklerinde (Allectoris gracea) bazı hematolojik parametreler. Veteriner Bilimleri Dergisi. Eurasian Journal of Veterinary Sciences, 18(3-4), 23-27.
- Kırıkçı, K., & Çetin, O. (1999). Keklik yetiştiriciliği. Türk Vet Hek Derg, 11(1-2), 15-18.
- Macit, S., & Akbulut, G. (2015). Diabetes mellitus ve oksidatif stres. Beslenme ve Diyet Dergisi, 43(1), 59-65.
- Özbey, O., & Esen, F. (2007). The effects of breeding systems and stocking density on some blood parameters of rock partridges (Alectoris graeca). Poultry science, 86(2), 420-422.
- Özçelik, M. (1995). Kuşlar Dünyası, Bilim ve Teknik. 328, 66- 73.
- Özek K, Bahtiyarca Y. (2004). Effects of sex and protein and energy levels in the diet on the blood parameters of the chukar partridge (Alectoris chukar). Br Poult Sci, 45(2): 290-293).
- Özhan N, Şimşek Ü.G., Özçelik M. (2016). Comparison of floor and cage housing systems in terms of some welfare assessments in broiler, Ankara Üniv Vet Fak Derg, 63, 317-322.
- Perez-Rodriguez, L., Mougeot, F., Alonso-Alvarez, C., Blas, J., Viñuela, J., & Bortolotti, G. R. (2008). Cell-mediated immune activation rapidly decreases plasma carotenoids but does not affect oxidative stress in red-legged partridges (Alectoris rufa). Journal of Experimental Biology, 211(13), 2155-2161.
- Sinha, A. K. (1972). Colorimetric assay of catalase. Analytical biochemistry, 47(2), 389-394.
- Storz, G., & Imlayt, J. A. (1999). Oxidative stress. Current opinion in microbiology, 2(2), 188-194.
- Sun, Y. I., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 34(3), 497-500.
- Suzuki, J., & Katoh, N. (1990). A simple and cheap methods for measuring serum vitamin A in cattle using only a spectrophotometer. The Japanese Journal of Veterinary Science, 52(6), 1281–1283.
- Şimşek, Ü. G., Erişir, M., Çiftçi, M., & Seven, P. T. (2014). Effects of cage and floor housing systems on fattening performance, oxidative stress and carcass defects in broiler chicken. Kafkas Vet Fak Derg, 20, 727-733.
- Valko M., D. Leibfritz, J. Moncola, M.T.D. Cronin, M. Mazura, and J. Telser. (2007). Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84.
- Winterbourn CC, Hawkins RE, Brian M, Carrell RW.(1975). The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine. 85(2): 337-341.
- Yılmaz B. (2000). Kan “Fizyoloji" 116- 135, Feryal Matbaacılık, Ankara.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Biochemistry, Veterinary Surgery |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | - |
| Publication Date | September 30, 2023 |
| Acceptance Date | August 28, 2023 |
| Published in Issue | Year 2023 Volume: 16 Issue: 3 |
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