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Bal ve Momordica charantia Linn'deki Fenolik Bileşiklerin Karşılaştırmalı Analizi

Year 2023, Volume: 16 Issue: 4, 556 - 562, 31.12.2023

Nilay Keyvan

https://doi.org/10.30607/kvj.1358936

Abstract

Fenolik bileşikler, hem balda hem de Momordica charantia'da bulunan ve potansiyel sağlık yararına katkıda bulunan biyoaktif bileşenlerdir. Doğal bir tatlandırıcı olan bal, antioksidan aktivitesine ve potansiyel tedavi edici özelliklerine katkıda bulunan fenolik asitleri, flavonoidleri ve diğer küçük fenolik içerikleri içerir. Bu çalışmada Momordica charantia Linn ve balın fenolik içeriğini belirlemek için Yüksek Performanslı Sıvı Kromatografisi (HPLC) tekniğinden yararlanılmıştır. Momordica charantia Linn'in analizi, en yüksek düzeyde p-hidroksi benzoik asit içerdiğini, en düşük miktarda ise p-kumarik asitin tespit edildiğini göstermiştir. Balın bileşiminde rutin birincil bileşen olarak tanımlanırken, en düşük konsantrasyonda o-kumarik asit tespit edilmiştir. Sonuç olarak Momordica charantia Linn ve balın önemli miktarda fenolik bileşik içerdiği sonucuna varılabilir.

Keywords

Bal Fenolik Bileşikler Kudret Narı Momordica charantia Linn

References

  • Busuioc, A., Botezatu, A., Furdui, B., Vînătoru, C., Maggi, F., Caprioli, G., & Dinica, R. (2020). Comparative Study Of the Chemical Compositions And Antioxidant Activities Of Fresh Juices From Romanian Cucurbitaceae Varieties. Molecules, 22(25), 5468-5485. https://doi.org/10.3390/molecules25225468
  • Dia, V. P., & Krishnan, H. B. (2016). BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells. Scientific Reports, 6(1), 33532. https://doi.org/10.1038/srep33532
  • Estevinho, L., Pereira, A., Moreira, L., Dias, L., & Pereira, E. (2008). Antioxidant and Antimicrobial Effects of Phenolic Compounds Extracts of Northeast Portugal Honey. Food and Chemical Toxicology, 12(46), 3774-3779. https://doi.org/10.1016/j.fct.2008.09.062
  • Giuliani, C., Tani, C., & Maleci Bini, L. (2016). Micromorphology and anatomy of fruits and seeds of bitter melon (Momordica charantia L., Cucurbitaceae). Acta Societatis Botanicorum Poloniae, 85(1), 1-7. https://doi.org/10.5586/asbp.3490
  • Haque, M. E., Alam, M. B., & Hossain, M. S. (2011). The efficacy of cucurbitane type triterpenoids, glycosides and phenolic compounds isolated from Momordica charantia: a review. International Journal of Pharmaceutical Sciences and Research, 2(5), 1135-1146.
  • Horax, R., Hettiarachchy, N., & Chen, P. (2010). Extraction, quantification, and antioxidant activities of phenolics from pericarp and seeds of bitter melons (Momordica charantia) harvested at three maturity stages (immature, mature, and ripe). Journal of Agricultural and Food Chemistry, 58(7), 4428-4433. https://doi.org/10.1021/jf9029578
  • Huang, W., Tsai, T., Huang, C., Li, Y., Chyuan, J., Chuang, L., & Tsai, P. (2015). Inhibitory Effects of Wild Bitter Melon Leaf Extract On Propionibacterium Acnes-induced Skin Inflammation In Mice and Cytokine Production In Vitro. Food and Function., 8(6), 2550-2560. https://doi.org/10.1039/c5fo00550g
  • Huang, W., Zhang, H., Liu, W., & Li, C. (2012). Survey of Antioxidant Capacity and Phenolic Composition of Blueberry, Blackberry, and Strawberry In Nanjing. Journal of Zhejiang University Science B, 2(13), 94-102. https://doi.org/10.1631/jzus.b1100137
  • Jiang, L., Xie, M., Chen, G., Qiao, J., Zhang, H., & Zeng, X. (2020). Phenolics and Carbohydrates In Buckwheat Honey Regulate The Human Intestinal Microbiota. Evidence-Based Complementary and Alternative Medicine, (2020), 1-11. https://doi.org/10.1155/2020/6432942
  • Kenny, O., Smyth, T. J., Hewage, C. M., & Brunton, N. P. (2013). Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chemistry, 141(4), 4295-4302. https://doi.org/10.1016/j.foodchem.2013.07.016
  • Kubola, J., & Siriamornpun, S. (2008). Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chemistry, 110(4), 881-890. https://doi.org/10.1016/j.foodchem.2008.02.076
  • McLoone, P., Warnock, M., & Fyfe, L. (2015). Honey: An Immunomodulatory Agent for Disorders of the Skin. Food and Agricultural Immunology, 27(3), 338-349. https://doi.org/10.1080/09540105.2015.1104653.
  • Meng, Y.; Liu, B.; Lei, N.; Zheng, J.; He, Q.; Li, D.; Zhao, X.; &Shen, F (2012). Alpha-momorcharin possessing high immunogenicity, immunotoxicity and hepatotoxicity in SD rats. Journal of Ethnopharmacology. 139, 590–598. https://doi.org/10.1016/j.jep.2011.11.057
  • Moniruzzaman, M., Khalil, I., Sulaiman, S., & Gan, S. (2013). Physicochemical and Antioxidant Properties of Malaysian Honeys Produced By Apis Cerana, Apis Dorsata and Apis Mellifera. BMC Complementary Medicine and Therapies, 13, 43. https://doi.org/10.1186/1472-6882-13-43
  • Muronga, M., Quispe, C., Tshikhudo, P., Msagati, T., Mudau, F., Sureda, A., & Sharifi-Rad, J. (2021). Three Selected Edible Crops Of the Genus Momordica As Potential Sources Of Phytochemicals: Biochemical, Nutritional, And Medicinal Values. Frontiers in Pharmacology., 12, 1-19. https://doi.org/10.3389/fphar.2021.625546
  • Nardini, M., Cirillo, E., Natella, F., & Scaccini, C. (2002). Absorption of Phenolic Acids In Humans After Coffee Consumption. Journal of Agricultural and Food Chemistry, 50(20), 5735-5741. https://doi.org/10.1021/jf0257547
  • Ng, Z., & Kuppusamy, U. (2019). Effects of Different Heat Treatments on the Antioxidant Activity and Ascorbic Acid Content of Bitter Melon, Momordica charantia. Brazilian Journal of Food Technology., 22, 1-9. https://doi.org/10.1590/1981-6723.28318.
  • Sarker, S., & Oba, S. (2019). Antioxidant Constituents of Three Selected Red and Green Color Amaranthus Leafy Vegetable. Scientific Reports, 9, 18233. https://doi.org/10.1038/s41598-019-52033-8
  • Owusu, E., Ahorlu, M., Afutu, E., Akumwena, A., & Asare, G. (2021). Antimicrobial Activity of Selected Medicinal Plants From a Sub-saharan African Country Against Bacterial Pathogens From Post-operative Wound Infections. Medical Sciences, 9(2), 23-39. https://doi.org/10.3390/medsci9020023
  • Prasad, V.; Jain, V.; Girish, D.; & Dorle, A.K. (2006). Wound-healing property of Momordica charantia L. fruit powder. Journal of Herbal Pharmacotherapy, 6, 105–115. https://doi.org/10.1080/J157v06n03_05
  • Pundir, V., & Chandel, A. (2021). Momordica Charantia Constituents and The Anti-proliferative Activity Against An Ovarian Cancer Cell-line Skov-3. Flora and Fauna, 27(2), 185-190. https://doi.org/10.33451/florafauna.v27i2pp185-190
  • Qader, S., Abdulla, M., Chua, L., Najim, N., Zain, M., Hamdan, S. (2011). Antioxidant, Total Phenolic Content and Cytotoxicity Evaluation of Selected Malaysian Plants. Molecules, 16(4), 3433-3443. https://doi.org/10.3390/molecules16043433
  • Sagor, A., Chowdhury, M., Tabassum, N., Hossain, H., Rahman, M., Alam, A. (2015). Supplementation of Fresh Ucche (Momordica Charantia L. Var. Muricata Willd) Prevented Oxidative Stress, Fibrosis and Hepatic Damage In Ccl4 Treated Rats. BMC Complementary Medicine and Therapies, 15(1), 115. https://doi.org/10.1186/s12906-015-0636-1
  • Sato, Y., Itagaki, S., Kurokawa, T., Ogura, J., Kobayashi, M., Hirano, T., & Iseki, K. (2011). In Vitro and In Vivo Antioxidant Properties of Chlorogenic Acid and Caffeic Acid. International Journal of Pharmaceutics, 403(1-2), 136-138. https://doi.org/10.1016/j.ijpharm.2010.09.035
  • Semiz, A.; & Sen, A. (2007). Antioxidant and chemoprotective properties of Momordica charantia L. (bitter melon) fruit extract. African Journal of Biotechnology, 6, 273–277.
  • Sood A, Kaur P, & Gupta R. (2012). Phytochemical screening and antimicrobial assay of various seeds extract of Cucurbitaceae family. International Journal of Applied Biology and Pharmaceutical Technology, 3, 401–409.
  • Tan, S. P., Kha, T. C., Parks, S. E., & Roach, P. D. (2016). Bitter melon (Momordica charantia L.) bioactive composition and health benefits: A review. Food Reviews International, 32(2), 181-202.
  • Tan, S., Stathopoulos, C., Parks, S., & Roach, P. (2014). An Optimised Aqueous Extract of Phenolic Compounds From Bitter Melon With High Antioxidant Capacity. Antioxidants, 4(3), 814-829. https://doi.org/10.3390/antiox3040814
  • Wadi, M. (2022). Bioactivity and Chemical Characterization of Sudanese Bee Honey: Crude Acacia And Its Organic Extracts. BioMed Research International, (2022), 1-9. https://doi.org/10.1155/2022/8441239
  • Yasui, Y.; Hosokawa, M.; Sahara, T.; Suzuki, R.; Ohgiya, S.; Kohno, H.; Tanaka, T.; Miyashita, K. (2005). Bitter gourd seed fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARγ in human colon cancer Caco-2 cells. Prostaglandins, Leukotrienes and Essential Fatty Acids, 73, 113–119. https://doi.org/10.1016/j.plefa.2005.04.013
  • Zamri, N., Ghani, N., Ismail, C., Zakaria, R., Shafin, N. (2023). Honey on Brain Health: a Promising Brain Booster. Front. Aging Neurosci., 14, 1-14. https://doi.org/10.3389/fnagi.2022.1092596

Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn

Year 2023, Volume: 16 Issue: 4, 556 - 562, 31.12.2023

Nilay Keyvan

https://doi.org/10.30607/kvj.1358936

Abstract

Phenolic compounds are bioactive constituents in honey and Momordica charantia, contributing to their potential health benefits. Honey, a natural sweetener, contains phenolic acids, flavonoids, and other minor phenolic contents, contributing to its antioxidant activity and potential therapeutic properties. In this study, the High-Performance Liquid Chromatography (HPLC) technique was utilized to determine the phenolic content of Momordica charantia Linn and honey. The analysis of Momordica charantia Linn demonstrated that it showed the highest level of p-hydroxy benzoic acid, whereas the lowest quantity of p-coumaric acid was detected. Rutin was identified as the primary constituent in the composition of honey, whereas o-coumaric acid was detected in the lowest concentration. In conclusion, it can be concluded that Momordica charantia Linn and honey possess a significant abundance of phenolic compounds.

Keywords

Bitter melon Honey Phenolic compounds Momordica charantia Linn

References

  • Busuioc, A., Botezatu, A., Furdui, B., Vînătoru, C., Maggi, F., Caprioli, G., & Dinica, R. (2020). Comparative Study Of the Chemical Compositions And Antioxidant Activities Of Fresh Juices From Romanian Cucurbitaceae Varieties. Molecules, 22(25), 5468-5485. https://doi.org/10.3390/molecules25225468
  • Dia, V. P., & Krishnan, H. B. (2016). BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells. Scientific Reports, 6(1), 33532. https://doi.org/10.1038/srep33532
  • Estevinho, L., Pereira, A., Moreira, L., Dias, L., & Pereira, E. (2008). Antioxidant and Antimicrobial Effects of Phenolic Compounds Extracts of Northeast Portugal Honey. Food and Chemical Toxicology, 12(46), 3774-3779. https://doi.org/10.1016/j.fct.2008.09.062
  • Giuliani, C., Tani, C., & Maleci Bini, L. (2016). Micromorphology and anatomy of fruits and seeds of bitter melon (Momordica charantia L., Cucurbitaceae). Acta Societatis Botanicorum Poloniae, 85(1), 1-7. https://doi.org/10.5586/asbp.3490
  • Haque, M. E., Alam, M. B., & Hossain, M. S. (2011). The efficacy of cucurbitane type triterpenoids, glycosides and phenolic compounds isolated from Momordica charantia: a review. International Journal of Pharmaceutical Sciences and Research, 2(5), 1135-1146.
  • Horax, R., Hettiarachchy, N., & Chen, P. (2010). Extraction, quantification, and antioxidant activities of phenolics from pericarp and seeds of bitter melons (Momordica charantia) harvested at three maturity stages (immature, mature, and ripe). Journal of Agricultural and Food Chemistry, 58(7), 4428-4433. https://doi.org/10.1021/jf9029578
  • Huang, W., Tsai, T., Huang, C., Li, Y., Chyuan, J., Chuang, L., & Tsai, P. (2015). Inhibitory Effects of Wild Bitter Melon Leaf Extract On Propionibacterium Acnes-induced Skin Inflammation In Mice and Cytokine Production In Vitro. Food and Function., 8(6), 2550-2560. https://doi.org/10.1039/c5fo00550g
  • Huang, W., Zhang, H., Liu, W., & Li, C. (2012). Survey of Antioxidant Capacity and Phenolic Composition of Blueberry, Blackberry, and Strawberry In Nanjing. Journal of Zhejiang University Science B, 2(13), 94-102. https://doi.org/10.1631/jzus.b1100137
  • Jiang, L., Xie, M., Chen, G., Qiao, J., Zhang, H., & Zeng, X. (2020). Phenolics and Carbohydrates In Buckwheat Honey Regulate The Human Intestinal Microbiota. Evidence-Based Complementary and Alternative Medicine, (2020), 1-11. https://doi.org/10.1155/2020/6432942
  • Kenny, O., Smyth, T. J., Hewage, C. M., & Brunton, N. P. (2013). Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chemistry, 141(4), 4295-4302. https://doi.org/10.1016/j.foodchem.2013.07.016
  • Kubola, J., & Siriamornpun, S. (2008). Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chemistry, 110(4), 881-890. https://doi.org/10.1016/j.foodchem.2008.02.076
  • McLoone, P., Warnock, M., & Fyfe, L. (2015). Honey: An Immunomodulatory Agent for Disorders of the Skin. Food and Agricultural Immunology, 27(3), 338-349. https://doi.org/10.1080/09540105.2015.1104653.
  • Meng, Y.; Liu, B.; Lei, N.; Zheng, J.; He, Q.; Li, D.; Zhao, X.; &Shen, F (2012). Alpha-momorcharin possessing high immunogenicity, immunotoxicity and hepatotoxicity in SD rats. Journal of Ethnopharmacology. 139, 590–598. https://doi.org/10.1016/j.jep.2011.11.057
  • Moniruzzaman, M., Khalil, I., Sulaiman, S., & Gan, S. (2013). Physicochemical and Antioxidant Properties of Malaysian Honeys Produced By Apis Cerana, Apis Dorsata and Apis Mellifera. BMC Complementary Medicine and Therapies, 13, 43. https://doi.org/10.1186/1472-6882-13-43
  • Muronga, M., Quispe, C., Tshikhudo, P., Msagati, T., Mudau, F., Sureda, A., & Sharifi-Rad, J. (2021). Three Selected Edible Crops Of the Genus Momordica As Potential Sources Of Phytochemicals: Biochemical, Nutritional, And Medicinal Values. Frontiers in Pharmacology., 12, 1-19. https://doi.org/10.3389/fphar.2021.625546
  • Nardini, M., Cirillo, E., Natella, F., & Scaccini, C. (2002). Absorption of Phenolic Acids In Humans After Coffee Consumption. Journal of Agricultural and Food Chemistry, 50(20), 5735-5741. https://doi.org/10.1021/jf0257547
  • Ng, Z., & Kuppusamy, U. (2019). Effects of Different Heat Treatments on the Antioxidant Activity and Ascorbic Acid Content of Bitter Melon, Momordica charantia. Brazilian Journal of Food Technology., 22, 1-9. https://doi.org/10.1590/1981-6723.28318.
  • Sarker, S., & Oba, S. (2019). Antioxidant Constituents of Three Selected Red and Green Color Amaranthus Leafy Vegetable. Scientific Reports, 9, 18233. https://doi.org/10.1038/s41598-019-52033-8
  • Owusu, E., Ahorlu, M., Afutu, E., Akumwena, A., & Asare, G. (2021). Antimicrobial Activity of Selected Medicinal Plants From a Sub-saharan African Country Against Bacterial Pathogens From Post-operative Wound Infections. Medical Sciences, 9(2), 23-39. https://doi.org/10.3390/medsci9020023
  • Prasad, V.; Jain, V.; Girish, D.; & Dorle, A.K. (2006). Wound-healing property of Momordica charantia L. fruit powder. Journal of Herbal Pharmacotherapy, 6, 105–115. https://doi.org/10.1080/J157v06n03_05
  • Pundir, V., & Chandel, A. (2021). Momordica Charantia Constituents and The Anti-proliferative Activity Against An Ovarian Cancer Cell-line Skov-3. Flora and Fauna, 27(2), 185-190. https://doi.org/10.33451/florafauna.v27i2pp185-190
  • Qader, S., Abdulla, M., Chua, L., Najim, N., Zain, M., Hamdan, S. (2011). Antioxidant, Total Phenolic Content and Cytotoxicity Evaluation of Selected Malaysian Plants. Molecules, 16(4), 3433-3443. https://doi.org/10.3390/molecules16043433
  • Sagor, A., Chowdhury, M., Tabassum, N., Hossain, H., Rahman, M., Alam, A. (2015). Supplementation of Fresh Ucche (Momordica Charantia L. Var. Muricata Willd) Prevented Oxidative Stress, Fibrosis and Hepatic Damage In Ccl4 Treated Rats. BMC Complementary Medicine and Therapies, 15(1), 115. https://doi.org/10.1186/s12906-015-0636-1
  • Sato, Y., Itagaki, S., Kurokawa, T., Ogura, J., Kobayashi, M., Hirano, T., & Iseki, K. (2011). In Vitro and In Vivo Antioxidant Properties of Chlorogenic Acid and Caffeic Acid. International Journal of Pharmaceutics, 403(1-2), 136-138. https://doi.org/10.1016/j.ijpharm.2010.09.035
  • Semiz, A.; & Sen, A. (2007). Antioxidant and chemoprotective properties of Momordica charantia L. (bitter melon) fruit extract. African Journal of Biotechnology, 6, 273–277.
  • Sood A, Kaur P, & Gupta R. (2012). Phytochemical screening and antimicrobial assay of various seeds extract of Cucurbitaceae family. International Journal of Applied Biology and Pharmaceutical Technology, 3, 401–409.
  • Tan, S. P., Kha, T. C., Parks, S. E., & Roach, P. D. (2016). Bitter melon (Momordica charantia L.) bioactive composition and health benefits: A review. Food Reviews International, 32(2), 181-202.
  • Tan, S., Stathopoulos, C., Parks, S., & Roach, P. (2014). An Optimised Aqueous Extract of Phenolic Compounds From Bitter Melon With High Antioxidant Capacity. Antioxidants, 4(3), 814-829. https://doi.org/10.3390/antiox3040814
  • Wadi, M. (2022). Bioactivity and Chemical Characterization of Sudanese Bee Honey: Crude Acacia And Its Organic Extracts. BioMed Research International, (2022), 1-9. https://doi.org/10.1155/2022/8441239
  • Yasui, Y.; Hosokawa, M.; Sahara, T.; Suzuki, R.; Ohgiya, S.; Kohno, H.; Tanaka, T.; Miyashita, K. (2005). Bitter gourd seed fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARγ in human colon cancer Caco-2 cells. Prostaglandins, Leukotrienes and Essential Fatty Acids, 73, 113–119. https://doi.org/10.1016/j.plefa.2005.04.013
  • Zamri, N., Ghani, N., Ismail, C., Zakaria, R., Shafin, N. (2023). Honey on Brain Health: a Promising Brain Booster. Front. Aging Neurosci., 14, 1-14. https://doi.org/10.3389/fnagi.2022.1092596
There are 31 citations in total.

Details

Primary Language English
Subjects Veterinary Food Hygiene and Technology
Journal Section RESEARCH ARTICLE
Authors

Nilay Keyvan 0000-0002-6717-2793

Early Pub Date December 20, 2023
Publication Date December 31, 2023
Acceptance Date November 30, 2023
Published in Issue Year 2023 Volume: 16 Issue: 4

Cite

APA Keyvan, N. (2023). Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn. Kocatepe Veterinary Journal, 16(4), 556-562. https://doi.org/10.30607/kvj.1358936
AMA Keyvan N. Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn. kvj. December 2023;16(4):556-562. doi:10.30607/kvj.1358936
Chicago Keyvan, Nilay. “Comparative Analysis of Phenolic Compounds in Honey and Momordica Charantia Linn”. Kocatepe Veterinary Journal 16, no. 4 (December 2023): 556-62. https://doi.org/10.30607/kvj.1358936.
EndNote Keyvan N (December 1, 2023) Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn. Kocatepe Veterinary Journal 16 4 556–562.
IEEE N. Keyvan, “Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn”, kvj, vol. 16, no. 4, pp. 556–562, 2023, doi: 10.30607/kvj.1358936.
ISNAD Keyvan, Nilay. “Comparative Analysis of Phenolic Compounds in Honey and Momordica Charantia Linn”. Kocatepe Veterinary Journal 16/4 (December 2023), 556-562. https://doi.org/10.30607/kvj.1358936.
JAMA Keyvan N. Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn. kvj. 2023;16:556–562.
MLA Keyvan, Nilay. “Comparative Analysis of Phenolic Compounds in Honey and Momordica Charantia Linn”. Kocatepe Veterinary Journal, vol. 16, no. 4, 2023, pp. 556-62, doi:10.30607/kvj.1358936.
Vancouver Keyvan N. Comparative Analysis of Phenolic Compounds in Honey and Momordica charantia Linn. kvj. 2023;16(4):556-62.
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