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Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis

Year 2021, Volume: 9 Issue: 3, 29 - 37, 22.12.2021

Necmettin Sarp Sevgisunar Şima Şahinduran

https://doi.org/10.24998/maeusabed.996891

Abstract

Johne’s Disease or paratuberculosis is a mycobacterial infection of ruminants and has a global economical impact. Mycobacterium avium subsp. paratuberculosis is the cause of this disease. It reduces milk production, cause chronic weight loss leading death and major losses. Acute phase reactions are defined as minimum 25% increase or decrease in serum concentrations of acute phase proteins which are triggered by pro or antiinflammatory cytokines released from various cells or tissues. The aim of the study was to investigate the effect of Mycobacterium avium subsp paratuberculosis on blood parameters, some acute phase proteins, cytokines and hepcidin in naturally infected goats with paratuberculosis. In this study, total 45 Saanen goats aged 2-5 years from both sex were used as animal material. Study group were included 35 and control group were included 10 animal for evaluation. Complete blood counts were performed on blood taken from all animals. Also interleukin 6, interleukin 10, serum amyloid A, haptoglobin, fibrinogen, and hepcidin levels were evaluated from serum samples. As a result, interleukin 6 (p<0.01), interleukin 10 (p<0.01) serum amyloid A (p<0.001), haptoglobin (p<0.001), fibrinogen (p<0.05), and hepcidin (p<0.05) differences between groups were statistically important and they can be used to support the diagnosis of paratuberculosis.

Keywords

Acute Phase proteins Cytokine Hepcidin Paratuberculosis Saanen goat.

Supporting Institution

Burdur Mehmet Akif Ersoy Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Project Number

0259-DR-15

Thanks

This study was supported by Mehmet Akif Ersoy University Scientific Research Projects Commission (Project No: 0259-DR-15).

References

  • Albay, M.K., Karakurum, M.C., Sahinduran, S., Sezer, K., Yildiz, R., Buyukoglu, T., 2014. Selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia. Veterinary Medicine Journal-Czech 59(7), 336-342.
  • Ameta, B.N., Hosseini, A., Odhiambo, J.F., Iqbal, S., Sharma, S., Deng, Q., Lam, T.H., Farooq, U., Zebeli, Q., Dunn, S.M., 2011. Application of acute phase proteins for monitoring inflammatory states in cattle. Ed: VEAS F, Acute phase proteins as early non-specific biomarkers of human and veterinary diseases, Croatia 299-354.
  • Arnold, J., Sangwaiya, A., Manglam, V., Geoghegan, F., Thursz, M., Busbridge, M., 2010. Presence of hepcidin-25 in biological fluids: bile, ascitic and pleural fluids. World Jounal of Gastroenterology 16, 2129–2133.
  • Ceciliani, F., Ceron, J.J., Eckersall, P.D., Sauerwein, H., 2012. Acute phase proteins in ruminants. Journal of Proteomics 75, 4207-4231.
  • Ceron, J.J., Eckersall, P.D., Martinez-Subiela, S., 2005. Acute phase proteins in dogs and cats: current knowledge and future perspectives. Veterinary Clinical Pathology 34(2), 85-99.
  • Chiodini, R.J., Van Kruiningen, H.J., Merkal, R.S., 1984. Ruminant paratuberculosis (Johne’s disease): the current status and future prospects. The Cornell Veterinarian 74, 218-262.
  • Conner, J.G., Eckersall, P.D., Wiseman, A., Aitchison, T.C., Douglas, T.A. 1998. Bovine acute phase response following turpentine injection. Research in Veterinary Science 44, 82–88.
  • Cray, C., Zaias, J., Altman, N.H., 2009. Acute phase response in animals: A review. Comparative Medicine 59(6), 517-526.
  • De Silva, K., Begg, D., Whittington, R., 2011. The interleukin 10 response in ovine Johne’s disease. Veterinary Immunology and Immunopathology 139, 10–16.
  • Eckersall, P.D., 2000. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue de Médecine Vétérinaire- Toulouse., 151(7), 577-584.
  • El-Deeb, W.M., 2013. Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers. Journal of Zhejiang University. Science. B 14, 33–39.
  • Fasulkov, I., Karadaev, M., Vasilev, N., Urumova, V., Mircheva, T., 2014. Determination of plasma fibrinogen and haptoglobin, hematological and blood biochemical changes in Bulgarian local goats with experimentally induced Staphylococcus aureus mastitis. Turkish Journal of Veterinary & Animal Sciences 38, 439-444.
  • Gonzalez, F.H.D., Tacles, F., Martinez-Subiela, S., Tvarijonaviciute, A., Soler, L., Ceron, J.J., 2008. Acute phase protein response in goats. J Vet Diagn Invest., 20, 580–584.
  • Gruys, E., Toussaint. M.J.M., Niewold, T.A., Koopmans, S.J., 2005. Acute phase reaction and acute phase proteins. Journal of Zhejiang University. Science A 6 (11), 1045-1056.
  • Hajimohammadi, A., Nazifi, S., Ansari-Lari, M., Khoshmanzar, M.R., Bigdeli, S.M., 2013. Identifying relationships among acute phase proteins (haptoglobin, serum amyloid A, fibrinogen, ceruloplasmin) and clinical findings in dairy calf diarrhea. Comparative Clinical Pathology 22(2), 227-232.
  • Harris, N.B., Barletta, R.G., 2001. Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clinical Microbiology Reviews 14(3), 489–512.
  • Heller, M.C., Johns, J.L., 2015. Acute phase proteins in healthy goats: establishment of reference intervals. Journal of Veterinary Diagnostic Investigation 27(2), 177-181.
  • Hirvonen, J., 2000. Hirvonen’s thesis on acute phase response in dairy cattle, Ed: Pyörälä S, University of Helsinki Faculty of Veterinary Medicine Publications, Helsinki 7-67.
  • Jeber, Z.K.H., Mohdjin, Z., Jesse, F.F., Saharee, A.A., Sabri, J., Yusoff, R., Wahid, H., 2016. Influence of Corynebacterium pseudotuberculosis infection on level of acute phase proteins in goats. BMC Veterinary Research., 12(48), 1-5.
  • Kali, A., Charles, M.V.P., Seetharam, R.S.K., 2015. Hepcidin-A novel biomarker with changing trends. Pharmacognosy Reviews 9(17), 35-40.
  • Kaneko, J.J., Harvey, J.W., Bruss, M.L., 2008. Clinical Biochemistry of Domestic Animals, 6th edition. Saunders, Philadelphia, USA.
  • Kemna, E.H., Tjalsma, H., Willems, H.L., Swinkels, D.W., 2008. Hepcidin: from discovery to differential diagnosis. Haematologica 93, 90–97.
  • Kulaksiz, H., Fein, E., Redecker, P., Stremmel, W., Adler, G., Cetin, Y., 2008. Pancreatic beta-cells express hepcidin, an iron-uptake regulatory peptide. Journal of Endocrinology 197, 241-249.
  • Laftah, A.H., Ramesh, B., Simpson, R.J., Solanky N, Bahram S, Schumann K, Debnam ES, Sari SKS (2004): Effect of hepcidin on intestinal iron absorption in mice. Blood 103, 3940–3944.
  • Lybeck, K.R., Storset, A.K., Djønne, B., Valheim, M., Olsen, I., 2011. Faecal shedding detected earlier than immune responses in goats naturally infected with Mycobacterium avium subsp. paratuberculosis. Research in Veterinary Science 91, 32–39.
  • Lybeck, K.R., Løvoll, M., Johansen, T.B., Olsen, I., Storset, A.K., Valheim, M., 2013. Intestinal strictures, fibrous adhesions and high local interleukin-10 levels in goats infected naturally with Mycobacterium avium subsp. paratuberculosis. Journal of Comparative Pathology 148, 157-172.
  • Merle, U., Fein, E., Gehrke, S.G., Stremmel, W., Kulaksiz, H., 2007. The iron regulatory peptide hepcidin is expressed in the heart and regulated by hypoxia and inflammation. Endocrinology 148, 2663–2668.
  • Murata, H., Shimada, N., Yoshioka, M., 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. Veterinary Journal 168, 28–40.
  • Nemeth, E., Rivera, S., Gabayan, V., Keller. C., Taudorf, S., Pedersen, B.K., Ganz, T., 2004. IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. Journal of Clinical Investigation 113(9), 1271-1276.
  • Neurath, M.F., Finotto, S., 2011. IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer. Cytokine and Growth Factor Reviews 22, 83–89.
  • Nicolas, G., Viatte, L., Bennoun, M., Kahn, A., Vaulont, S., 2002. Hepcidin, a new iron regulatory peptide. Blood Cells, Molecules and Diseases 29, 327–335.
  • O’Garra, A., Murphy, K.M., 2009. From IL-10 to IL-12: how pathogens and their products stimulate APCs to induce T(H)1 development. Nature Immunology 10, 929–932.
  • Park, C.H., Valore, E.V., Waring, A.J., Ganz, T., 2001. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. Journal of Biological Chemistry 276, 7806–7810.
  • Radostits, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D., 2007. Veterinary Medicine. A textbook of the diseases of cattle, horse, sheep, pigs and goats, 10th Ed, Saunders Elsevier, Edinburgh.
  • Senturk, S., Metcitoglu, Z., Ulgen, M., Borum, E., Temizel, E., Kasap, S., 2009. Evaluation of serum iron and iron binding capacity in cows with paratuberculosis. Tierarztliche Praxis Ausgabe Grobtiere Nutztiere 37(6), 375-378.

Evaluation of Some Acute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis

Year 2021, Volume: 9 Issue: 3, 29 - 37, 22.12.2021

Necmettin Sarp Sevgisunar Şima Şahinduran

https://doi.org/10.24998/maeusabed.996891

Abstract

Johne’s Disease or paratuberculosis is a mycobacterial infection of ruminants and has a global economical impact. Mycobacterium avium subsp. paratuberculosis is the cause of this disease. It reduces milk production, cause chronic weight loss leading death and major losses. Acute phase reactions are defined as minimum 25% increase or decrease in serum concentrations of acute phase proteins which are triggered by pro or antiinflammatory cytokines released from various cells or tissues. The aim of the study was to investigate the effect of Mycobacterium avium subsp paratuberculosis on blood parameters, some acute phase proteins, cytokines and hepcidin in naturally infected goats with paratuberculosis. In this study, total 45 Saanen goats aged 2-5 years from both sex were used as animal material. Study group were included 35 and control group were included 10 animal for evaluation. Complete blood counts were performed on blood taken from all animals. Also interleukin 6, interleukin 10, serum amyloid A, haptoglobin, fibrinogen, and hepcidin levels were evaluated from serum samples. As a result, interleukin 6 (p<0.01), interleukin 10 (p<0.01) serum amyloid A (p<0.001), haptoglobin (p<0.001), fibrinogen (p<0.05), and hepcidin (p<0.05) differences between groups were statistically important and they can be used to support the diagnosis of paratuberculosis.

Keywords

Acute phase proteins cytokine hepcidin paratuberculosis

Project Number

0259-DR-15

References

  • Albay, M.K., Karakurum, M.C., Sahinduran, S., Sezer, K., Yildiz, R., Buyukoglu, T., 2014. Selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia. Veterinary Medicine Journal-Czech 59(7), 336-342.
  • Ameta, B.N., Hosseini, A., Odhiambo, J.F., Iqbal, S., Sharma, S., Deng, Q., Lam, T.H., Farooq, U., Zebeli, Q., Dunn, S.M., 2011. Application of acute phase proteins for monitoring inflammatory states in cattle. Ed: VEAS F, Acute phase proteins as early non-specific biomarkers of human and veterinary diseases, Croatia 299-354.
  • Arnold, J., Sangwaiya, A., Manglam, V., Geoghegan, F., Thursz, M., Busbridge, M., 2010. Presence of hepcidin-25 in biological fluids: bile, ascitic and pleural fluids. World Jounal of Gastroenterology 16, 2129–2133.
  • Ceciliani, F., Ceron, J.J., Eckersall, P.D., Sauerwein, H., 2012. Acute phase proteins in ruminants. Journal of Proteomics 75, 4207-4231.
  • Ceron, J.J., Eckersall, P.D., Martinez-Subiela, S., 2005. Acute phase proteins in dogs and cats: current knowledge and future perspectives. Veterinary Clinical Pathology 34(2), 85-99.
  • Chiodini, R.J., Van Kruiningen, H.J., Merkal, R.S., 1984. Ruminant paratuberculosis (Johne’s disease): the current status and future prospects. The Cornell Veterinarian 74, 218-262.
  • Conner, J.G., Eckersall, P.D., Wiseman, A., Aitchison, T.C., Douglas, T.A. 1998. Bovine acute phase response following turpentine injection. Research in Veterinary Science 44, 82–88.
  • Cray, C., Zaias, J., Altman, N.H., 2009. Acute phase response in animals: A review. Comparative Medicine 59(6), 517-526.
  • De Silva, K., Begg, D., Whittington, R., 2011. The interleukin 10 response in ovine Johne’s disease. Veterinary Immunology and Immunopathology 139, 10–16.
  • Eckersall, P.D., 2000. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue de Médecine Vétérinaire- Toulouse., 151(7), 577-584.
  • El-Deeb, W.M., 2013. Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers. Journal of Zhejiang University. Science. B 14, 33–39.
  • Fasulkov, I., Karadaev, M., Vasilev, N., Urumova, V., Mircheva, T., 2014. Determination of plasma fibrinogen and haptoglobin, hematological and blood biochemical changes in Bulgarian local goats with experimentally induced Staphylococcus aureus mastitis. Turkish Journal of Veterinary & Animal Sciences 38, 439-444.
  • Gonzalez, F.H.D., Tacles, F., Martinez-Subiela, S., Tvarijonaviciute, A., Soler, L., Ceron, J.J., 2008. Acute phase protein response in goats. J Vet Diagn Invest., 20, 580–584.
  • Gruys, E., Toussaint. M.J.M., Niewold, T.A., Koopmans, S.J., 2005. Acute phase reaction and acute phase proteins. Journal of Zhejiang University. Science A 6 (11), 1045-1056.
  • Hajimohammadi, A., Nazifi, S., Ansari-Lari, M., Khoshmanzar, M.R., Bigdeli, S.M., 2013. Identifying relationships among acute phase proteins (haptoglobin, serum amyloid A, fibrinogen, ceruloplasmin) and clinical findings in dairy calf diarrhea. Comparative Clinical Pathology 22(2), 227-232.
  • Harris, N.B., Barletta, R.G., 2001. Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clinical Microbiology Reviews 14(3), 489–512.
  • Heller, M.C., Johns, J.L., 2015. Acute phase proteins in healthy goats: establishment of reference intervals. Journal of Veterinary Diagnostic Investigation 27(2), 177-181.
  • Hirvonen, J., 2000. Hirvonen’s thesis on acute phase response in dairy cattle, Ed: Pyörälä S, University of Helsinki Faculty of Veterinary Medicine Publications, Helsinki 7-67.
  • Jeber, Z.K.H., Mohdjin, Z., Jesse, F.F., Saharee, A.A., Sabri, J., Yusoff, R., Wahid, H., 2016. Influence of Corynebacterium pseudotuberculosis infection on level of acute phase proteins in goats. BMC Veterinary Research., 12(48), 1-5.
  • Kali, A., Charles, M.V.P., Seetharam, R.S.K., 2015. Hepcidin-A novel biomarker with changing trends. Pharmacognosy Reviews 9(17), 35-40.
  • Kaneko, J.J., Harvey, J.W., Bruss, M.L., 2008. Clinical Biochemistry of Domestic Animals, 6th edition. Saunders, Philadelphia, USA.
  • Kemna, E.H., Tjalsma, H., Willems, H.L., Swinkels, D.W., 2008. Hepcidin: from discovery to differential diagnosis. Haematologica 93, 90–97.
  • Kulaksiz, H., Fein, E., Redecker, P., Stremmel, W., Adler, G., Cetin, Y., 2008. Pancreatic beta-cells express hepcidin, an iron-uptake regulatory peptide. Journal of Endocrinology 197, 241-249.
  • Laftah, A.H., Ramesh, B., Simpson, R.J., Solanky N, Bahram S, Schumann K, Debnam ES, Sari SKS (2004): Effect of hepcidin on intestinal iron absorption in mice. Blood 103, 3940–3944.
  • Lybeck, K.R., Storset, A.K., Djønne, B., Valheim, M., Olsen, I., 2011. Faecal shedding detected earlier than immune responses in goats naturally infected with Mycobacterium avium subsp. paratuberculosis. Research in Veterinary Science 91, 32–39.
  • Lybeck, K.R., Løvoll, M., Johansen, T.B., Olsen, I., Storset, A.K., Valheim, M., 2013. Intestinal strictures, fibrous adhesions and high local interleukin-10 levels in goats infected naturally with Mycobacterium avium subsp. paratuberculosis. Journal of Comparative Pathology 148, 157-172.
  • Merle, U., Fein, E., Gehrke, S.G., Stremmel, W., Kulaksiz, H., 2007. The iron regulatory peptide hepcidin is expressed in the heart and regulated by hypoxia and inflammation. Endocrinology 148, 2663–2668.
  • Murata, H., Shimada, N., Yoshioka, M., 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. Veterinary Journal 168, 28–40.
  • Nemeth, E., Rivera, S., Gabayan, V., Keller. C., Taudorf, S., Pedersen, B.K., Ganz, T., 2004. IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. Journal of Clinical Investigation 113(9), 1271-1276.
  • Neurath, M.F., Finotto, S., 2011. IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer. Cytokine and Growth Factor Reviews 22, 83–89.
  • Nicolas, G., Viatte, L., Bennoun, M., Kahn, A., Vaulont, S., 2002. Hepcidin, a new iron regulatory peptide. Blood Cells, Molecules and Diseases 29, 327–335.
  • O’Garra, A., Murphy, K.M., 2009. From IL-10 to IL-12: how pathogens and their products stimulate APCs to induce T(H)1 development. Nature Immunology 10, 929–932.
  • Park, C.H., Valore, E.V., Waring, A.J., Ganz, T., 2001. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. Journal of Biological Chemistry 276, 7806–7810.
  • Radostits, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D., 2007. Veterinary Medicine. A textbook of the diseases of cattle, horse, sheep, pigs and goats, 10th Ed, Saunders Elsevier, Edinburgh.
  • Senturk, S., Metcitoglu, Z., Ulgen, M., Borum, E., Temizel, E., Kasap, S., 2009. Evaluation of serum iron and iron binding capacity in cows with paratuberculosis. Tierarztliche Praxis Ausgabe Grobtiere Nutztiere 37(6), 375-378.
There are 35 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Necmettin Sarp Sevgisunar This is me 0000-0002-0392-1548

Şima Şahinduran 0000-0002-7718-2020

Project Number 0259-DR-15
Early Pub Date December 28, 2021
Publication Date December 22, 2021
Submission Date September 17, 2021
Published in Issue Year 2021 Volume: 9 Issue: 3

Cite

APA Sevgisunar, N. S., & Şahinduran, Ş. (2021). Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 9(3), 29-37. https://doi.org/10.24998/maeusabed.996891
AMA Sevgisunar NS, Şahinduran Ş. Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis. Mehmet Akif Ersoy University Journal of Health Sciences Institute. December 2021;9(3):29-37. doi:10.24998/maeusabed.996891
Chicago Sevgisunar, Necmettin Sarp, and Şima Şahinduran. “Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats With Paratuberculosis”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9, no. 3 (December 2021): 29-37. https://doi.org/10.24998/maeusabed.996891.
EndNote Sevgisunar NS, Şahinduran Ş (December 1, 2021) Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9 3 29–37.
IEEE N. S. Sevgisunar and Ş. Şahinduran, “Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 9, no. 3, pp. 29–37, 2021, doi: 10.24998/maeusabed.996891.
ISNAD Sevgisunar, Necmettin Sarp - Şahinduran, Şima. “Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats With Paratuberculosis”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 9/3 (December 2021), 29-37. https://doi.org/10.24998/maeusabed.996891.
JAMA Sevgisunar NS, Şahinduran Ş. Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2021;9:29–37.
MLA Sevgisunar, Necmettin Sarp and Şima Şahinduran. “Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats With Paratuberculosis”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 9, no. 3, 2021, pp. 29-37, doi:10.24998/maeusabed.996891.
Vancouver Sevgisunar NS, Şahinduran Ş. Evaluation of Some Ecute Phase Proteins, Cytokines and Hepcidin Levels in Naturally Infected Saanen Goats with Paratuberculosis. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2021;9(3):29-37.
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