Süt Sığırı Rasyonunun İn Vitro Ruminal Gaz Üretimi, Metan Salınımı, Organik Asit ve Protozoa Sayısı Üzerine Aquilaria agallocha Roxb. Uçucu Yağının Etkisi
Abstract
Ruminal fermantasyon sonucu oluşan ve sera gazı olan metan (CH4) hem ekonomik hem de ekolojik olarak küresel iklim değişikliğinde önemli paya sahiptir. Ruminant beslemede uçucu yağların metan emisyonlarını azalttığına yönelik çalışmalar bulunmaktadır. Bu in vitro çalışmanın amacı, Aquilaria agallocha Roxb. bitkisinden elde edilen uçucu yağın yonca kuru otunun ruminal gaz üretimini, metan emisyonunu, organik asitler ve protozoa üzerine etkilerini araştırmaktır. Aquilaria agallocha Roxb. uçucu yağının bileşenleri analiz edildi. Rumen sıvısına 5, 10 ve 15 µL/0,2 g kuru madde (KM) (A0, A5, A10 ve A15) seviyelerinde Aquilaria agallocha Roxb. uçucu yağı eklenmesinin in vitro ruminal sindirime etkileri in vitro gaz üretimi ile belirlendi. Sonuç olarak rumen sıvısına 10 µL Aquilaria agallocha Roxb. uçucu yağı eklenmesinin, yonca otunun in vitro ruminal gaz üretimi ve sindirim parametreleri üzerinde olumlu etki göstererek antimetanojenik etki yarattığı belirlendi.
References
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- Boyne AW., Eadıe JM., Raıtt K. The development and testing of a method of counting rumen ciliate protozoa. J Gen Microbiol 1957; 17(2): 414-23.
- Cobellis G., Trabalza MM., Marcotullio MC., Yu Z. Evaluation of different essential oils in modulating methane and ammonia production, rumen fermentation, and rumen bacteria in vitro. Anim Feed Sci Technology 2016; 215: 25-36.
- Ersahince C., Kara K. Nutrient composition and in vitro digestion parameters of Jerusalem artichoke (Helianthus tuberosus L.) herbage at different maturity stages in horse and ruminant. Journal of Animal and Feed Sciences 2017; 26(3): 213-225.
- Garcia F., Colombatto D., Brunetti MA., Martínez MJ., Moren MV., Scorcione TM., Martínez FJ. (2020). The reduction of methane production in the in vitro ruminal fermentation of different substrates is linked with the chemical composition of the essential oil. Animals, 10(5): 786.
- Gerber P., Hristov AN., Henderson B., Makkar H., Oh J., Lee C., Meinen R., Montes F., Ott T., Firkins J., A Rotz., Dell C., Adesogan AT., Yang WZ, Tricarico JM., Kebreab E., Waghorn G., Dijkstra J., Oosting S. Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: A review. Animal 2013; 2(2): 220-34.
- Ulger I., Kamalak A., Kurt O., Kaya E., Guven I. Comparación de la composición química y el potencial anti-metanogénico de las hojas de Liquidambar orientalis con hojas de Laurus nobilis y Eucalyptus globulus utilizando la técnica de producción de gas in vitro. Ciencia Invest Agraria 2017; 44 (1): 75-82.
- Joch M., Cermak L., Hakl J., Hucko B., Duskova D., Marounek M. In vitro screening of essential oil active compounds for manipulation of rumen fermentation and methane mitigation. Asian-Australasian journal of animal sciences 2016: 29(7), 952.
- Makkar HPS., Blümmel M., Becker K. In vitro rumen apparent and true digestibilities of tannin-rich forages. Anim Feed Sci Technology 1997; 67: 245-51.
- Mandal GP., Roy A., Patra AK. Effects of plant extracts rich in tannins, saponins and essential oils on rumen fermentation and conjugated linoleic acid conentrations in vitro. Research Article Indian J Anim 2016; 55: 49-60.
- Menke KH., Raab L., Salewski A., Steingass H., Fritz D., Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. The J Agricultural Sci 1979; 93(1): 217-22.
- ONEL SE., Taylan A., Kanber K., AKSU DS. (2020). The Effects of Laurel Volatile Oil (Laurusnobilis L.) on In Vitro Ruminal Gas Production of Methane Emission, Organic Acids and Protozoa Counts Alfalfa Herbage. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 17(3): 283-289.
- Patra AK., Yu Z. Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Appl Environ Microbiol 2012; 78(12): 4271-80.
- Schultz MM., Neser FW., Makgahlela ML. A balanced perspective on the importance of extensive ruminant production for human nutrition and livelihoods and its contribution to greenhouse gas emissions. South African Journal of Science 2020. 116(9-10):1-3.
- Tural S., Turhan S. Antimicrobial and antioxidant properties of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and laurel (Lauris nobilis L.) essential oils and their mixtures. Gida J of Food 2017; 42(5): 588-96.
- Zhou R., Wu J., Lang X., Liu L., Casper DP., Wang C., Wei S. Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community. J Dairy Sci 2020; 103(3): 2303-14.
The Effects of Aquilaria agallocha Roxb. Volatile Oil on In Vitro Ruminal Gas Production, Methane Emission, Volatile Fatty Acide Amounts and Protozoa Counts of Total Mixed Ration of Dairy Cattle
Abstract
Methane (CH4), a major greenhouse gas produced during ruminal fermentation, has a significant ratio in both the economic and ecological impact of global climate change. Literature reports have been published, which suggest that dietary supplementation with plant volatile oils reduces methane emissions in ruminant production. This in vitro study was aimed at investigating the effects of agarwood (Aquilaria agallocha Roxb.) volatile oil on in vitro ruminal gas production, methane emission, ruminal organic acids and protozoa counts of total mixed ration (TMR) of dairy cattle. Component analyses were performed for agarwood (Aquilaria agallocha Roxb.) volatile oil. The effects of agarwood volatile oil, added to ruminal fluid at levels of 5, 10 and 15 µL/0.2 g dry matter (DM) (Groups A0, A5, A10 and A15), on in vitro ruminal digestion were determined based on in vitro gas production. In conclusion, it was ascertained that the addition of 10 µL of Aquilaria agallocha Roxb. volatile oil to ruminal fluid positively affected in vitro ruminal gas production and digestion parameters of total mixed ration (TMR) of dairy cattle, and showed an antimethanogenic effect.
References
- Beauchemin KA., Ungerfeld EM., Eckard RJ., Wang M. Review: fifty years of research on rumen methanogenesis: lessons learned and future challenges for mitigation. Animal 2020; 14 (S1), S2–S16.
- Blümmel M., Makkar HPS., Becker K. In vitro gas production: A technique revisited. J Anim Phys Anim Nutr 1997; 77(1): 24-34.
- Boyne AW., Eadıe JM., Raıtt K. The development and testing of a method of counting rumen ciliate protozoa. J Gen Microbiol 1957; 17(2): 414-23.
- Cobellis G., Trabalza MM., Marcotullio MC., Yu Z. Evaluation of different essential oils in modulating methane and ammonia production, rumen fermentation, and rumen bacteria in vitro. Anim Feed Sci Technology 2016; 215: 25-36.
- Ersahince C., Kara K. Nutrient composition and in vitro digestion parameters of Jerusalem artichoke (Helianthus tuberosus L.) herbage at different maturity stages in horse and ruminant. Journal of Animal and Feed Sciences 2017; 26(3): 213-225.
- Garcia F., Colombatto D., Brunetti MA., Martínez MJ., Moren MV., Scorcione TM., Martínez FJ. (2020). The reduction of methane production in the in vitro ruminal fermentation of different substrates is linked with the chemical composition of the essential oil. Animals, 10(5): 786.
- Gerber P., Hristov AN., Henderson B., Makkar H., Oh J., Lee C., Meinen R., Montes F., Ott T., Firkins J., A Rotz., Dell C., Adesogan AT., Yang WZ, Tricarico JM., Kebreab E., Waghorn G., Dijkstra J., Oosting S. Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: A review. Animal 2013; 2(2): 220-34.
- Ulger I., Kamalak A., Kurt O., Kaya E., Guven I. Comparación de la composición química y el potencial anti-metanogénico de las hojas de Liquidambar orientalis con hojas de Laurus nobilis y Eucalyptus globulus utilizando la técnica de producción de gas in vitro. Ciencia Invest Agraria 2017; 44 (1): 75-82.
- Joch M., Cermak L., Hakl J., Hucko B., Duskova D., Marounek M. In vitro screening of essential oil active compounds for manipulation of rumen fermentation and methane mitigation. Asian-Australasian journal of animal sciences 2016: 29(7), 952.
- Makkar HPS., Blümmel M., Becker K. In vitro rumen apparent and true digestibilities of tannin-rich forages. Anim Feed Sci Technology 1997; 67: 245-51.
- Mandal GP., Roy A., Patra AK. Effects of plant extracts rich in tannins, saponins and essential oils on rumen fermentation and conjugated linoleic acid conentrations in vitro. Research Article Indian J Anim 2016; 55: 49-60.
- Menke KH., Raab L., Salewski A., Steingass H., Fritz D., Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. The J Agricultural Sci 1979; 93(1): 217-22.
- ONEL SE., Taylan A., Kanber K., AKSU DS. (2020). The Effects of Laurel Volatile Oil (Laurusnobilis L.) on In Vitro Ruminal Gas Production of Methane Emission, Organic Acids and Protozoa Counts Alfalfa Herbage. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 17(3): 283-289.
- Patra AK., Yu Z. Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Appl Environ Microbiol 2012; 78(12): 4271-80.
- Schultz MM., Neser FW., Makgahlela ML. A balanced perspective on the importance of extensive ruminant production for human nutrition and livelihoods and its contribution to greenhouse gas emissions. South African Journal of Science 2020. 116(9-10):1-3.
- Tural S., Turhan S. Antimicrobial and antioxidant properties of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and laurel (Lauris nobilis L.) essential oils and their mixtures. Gida J of Food 2017; 42(5): 588-96.
- Zhou R., Wu J., Lang X., Liu L., Casper DP., Wang C., Wei S. Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community. J Dairy Sci 2020; 103(3): 2303-14.
Details
| Primary Language | English |
|---|---|
| Subjects | Zootechny (Other) |
| Journal Section | RESEARCH ARTICLES |
| Authors | |
| Publication Date | December 15, 2021 |
| Submission Date | August 18, 2021 |
| Acceptance Date | October 27, 2021 |
| Published in Issue | Year 2021 Volume: 4 Issue: 3 |
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