The Effect of Thiol-Disulfide Homeostasis on Prognosis in Patients with Early-Stage Non-Small Cell Lung Cancer
Abstract
Objective: Lung cancer has a poor prognosis. Thiol groups with high antioxidant capacity are converted to disulfide bonds through biochemical reactions that neutralize different oxidant compounds. The thiol-disulfide (SH-SS) homeostasis has significant effects on cell mechanisms, transcription, and apoptosis. Here we present the prognostic role of dynamic SH-SS homeostasis in patients operated for NSCLC.
Materials and Methods: Patients operating for early-stage NSCLC were prospectively analyzed. SH-SS homeostasis tests were measured using the automated spectrophotometric method.
Results: This study enrolled 138 subjects, including 77 patients and 61 healthy controls. Native thiol and total thiol levels were significantly lower in the patient group. The disulfide-to-native thiol ratio, which is an indicator of oxidative stress in SH-SS homeostasis, also reached a level of statistical significance in the patient group (p<0.001). According to the cut-off values (305 and 326.3), the median overall survival rate was significantly shorter in patients with low native thiol and total thiol levels (p<0.001).
Conclusions: This study demonstrated decreased native thiol and total thiol levels as well as decreased disulfide levels and SS/SH ratio in early-stage NSCLC. Impaired SH-SS homeostasis may contribute to lung cancer pathogenesis and poor prognosis because of enhanced oxidative stress.
References
- 1. Şener MU, Sönmez Ö, Keyf İA, et al. Evaluation of Thiol/Disulfide Homeostasis in Lung Cancer. Turk Thorac J. 2020;21(4):255-260. doi: 10.5152/TurkThoracJ.2019.19033
- 2. Useros JM, Weiyao L, Morales MC, Foncillas JG. Oxidative Stress: A New Target for Pancreatic Cancer Prognosis and Treatment. J Clin Med. 2017;9;6(3):1-16. doi: 10.3390/jcm6030029.
- 3. Erenler AK, Yardan T. Clinical Utility of Thiol/Disulfide Homeostasis. Clin Lab. 2017; 63(5):867-870. doi: 10.7754/Clin.Lab.2017.161117
- 4. Erel O, Neselioglu S. A novel and automated assay for thiol/disulfide homeostasis. Clin Biochem. 2014;47(18):326–332.
- 5. Yazicioglu A, Ergin M, Koca C, Yekeler E, Erel O, Karaoglanoglu N. Does intrapleural sericin administration have an effect on rat plasma thiol disulfide redox homeostasis in accordance with antioxidative functions or cancer preventive features. Sch J App Med Sci. 2017;5(2C):476-482.
- 6. Beyoglu, MA, Sahin MF, Turkkan S, et al. Dynamic Thiol-Disulfide Homeostasis in Lung Transplant Recipients. Exp Clin Transplant. 2022; Online ahead of print. doi:10.6002/ect.2021.0360.
- 7. Demirseren DD, Cicek C, Alisik M, Demirseren ME, Aktaş A, Erel O. Dynamic thiol/disulfide homeostasis in patients with basal cell carcinoma. Cutan Ocul Toxicol. 2017;1:1-5.
- 8. Otal Y, Kahraman FA, Haydar FG, Erel Ö. Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis. Turk J Med Sci. 2021;51:743-748.
- 9. Erel Ö, Erdoğan S. Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects. Turk J Med Sci. 2020;50:1728-1738.
- 10. Georgescu SR, Mitran CJ, Mitran MI, et al. Thiol-Disulfide Homeostasis in Skin Diseases. J. Clin. Med. 2022;11:1507. doi.org/10.3390/jcm11061507
- 11. Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Report. 2016;21:(5)197-203.
Erken Evre Küçük Hücreli Dışı Akciğer Kanserli Hastalarda Tiyol-Disülfid Homeostazisin Prognoz Üzerine Etkisi
Abstract
Amaç: Akciğer kanseri kötü prognoza sahiptir. Antioksidan kapasitesi yüksek olan tiyol grupları, farklı oksidan bileşikleri nötralize eden biyokimyasal reaksiyonlarla disülfid (SS) gruplarına dönüşürler. Tiyol/Disülfid homeostazı (TDH) hücre mekanizmaları, transkripsiyon ve apoptoz üzerinde önemli etkilere sahiptir. KHDAK nedeniyle ameliyat edilen hastalarda dinamik TDH'nin prognozunun rolünü sunuyoruz.
Materyal ve Metot: KHDAK ile erken evre opere edilen hastalarda prospektif olarak analiz edildi. TDH testleri otomatik spektrofotometrik yöntemle ölçüldü.
Bulgular: Bu çalışmaya 77 hasta, 61 sağlıklı olmak üzere toplam 138 kişi katıldı. Native tiyol (NT) ve total tiyol (TT) düzeyleri hasta grubunda anlamlı olarak düşüktü. TDH'nin oksidatif stres göstergesi olan SS/NT oranı da hastalarda istatistiksel olarak anlamlı bulunmuştur (p<0,001). Medyan OS, NT ve TT düzeyi düşük olan hastalarda cut-off değerine göre (305 ve 326,3) anlamlı olarak daha kısaydı (p<0,001).
Sonuç: Bu sonuçlar erken evre KHDAK'de; doğal tiyol ve toplam tiyol seviyeleri azaldığını, disülfid seviyeleri ve disülfid/NT oranının da bozulduğunu göstermektedir. Bozulmuş tiyol/disülfid homeostazı, artan oksidatif stresin bir sonucu olarak akciğer kanseri patogenezine ve kötü prognoza katkıda bulunabilir.
References
- 1. Şener MU, Sönmez Ö, Keyf İA, et al. Evaluation of Thiol/Disulfide Homeostasis in Lung Cancer. Turk Thorac J. 2020;21(4):255-260. doi: 10.5152/TurkThoracJ.2019.19033
- 2. Useros JM, Weiyao L, Morales MC, Foncillas JG. Oxidative Stress: A New Target for Pancreatic Cancer Prognosis and Treatment. J Clin Med. 2017;9;6(3):1-16. doi: 10.3390/jcm6030029.
- 3. Erenler AK, Yardan T. Clinical Utility of Thiol/Disulfide Homeostasis. Clin Lab. 2017; 63(5):867-870. doi: 10.7754/Clin.Lab.2017.161117
- 4. Erel O, Neselioglu S. A novel and automated assay for thiol/disulfide homeostasis. Clin Biochem. 2014;47(18):326–332.
- 5. Yazicioglu A, Ergin M, Koca C, Yekeler E, Erel O, Karaoglanoglu N. Does intrapleural sericin administration have an effect on rat plasma thiol disulfide redox homeostasis in accordance with antioxidative functions or cancer preventive features. Sch J App Med Sci. 2017;5(2C):476-482.
- 6. Beyoglu, MA, Sahin MF, Turkkan S, et al. Dynamic Thiol-Disulfide Homeostasis in Lung Transplant Recipients. Exp Clin Transplant. 2022; Online ahead of print. doi:10.6002/ect.2021.0360.
- 7. Demirseren DD, Cicek C, Alisik M, Demirseren ME, Aktaş A, Erel O. Dynamic thiol/disulfide homeostasis in patients with basal cell carcinoma. Cutan Ocul Toxicol. 2017;1:1-5.
- 8. Otal Y, Kahraman FA, Haydar FG, Erel Ö. Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis. Turk J Med Sci. 2021;51:743-748.
- 9. Erel Ö, Erdoğan S. Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects. Turk J Med Sci. 2020;50:1728-1738.
- 10. Georgescu SR, Mitran CJ, Mitran MI, et al. Thiol-Disulfide Homeostasis in Skin Diseases. J. Clin. Med. 2022;11:1507. doi.org/10.3390/jcm11061507
- 11. Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Report. 2016;21:(5)197-203.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research article |
| Authors | |
| Early Pub Date | March 2, 2023 |
| Publication Date | March 5, 2023 |
| Submission Date | December 30, 2022 |
| Acceptance Date | February 5, 2023 |
| Published in Issue | Year 2023 Volume: 8 Issue: 1 |
