Research Article
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Year 2017, Volume: 1 Issue: 1, 1 - 6, 01.12.2017

Abstract

References

  • Altıntaş A, Fidancı UR, Sel T, Duru Ö, Başsatan A. Doğal ve Endüstriyel Florozisli Koyunlarda Böbrek Fonksiyonu ve Serum Protein Elektroforezi. Ankara Ü Vet Fak Derg 2000; 47:105-114.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J LabClinMed 1963; 61:882-90. Chance B, GreenStein DS, Roughton RJW. The mechanism of catalyse action steady state analysis. Arch Biochem Biophys 1982; 37:301–339.
  • Chaverri JP, Sánchez-Lozada LG, Osorio-Alonso H, Tapia E, Scholze A. New pathogenic concepts and the rapeutic approaches to oxidative stress in chronic kidney disease. Oxidative Medicine and Cellular Longevity 2016; 2016:1-21. Chlubek D,Poland S. Fluoride and oxidatif stress. Fluoride 2003;36(4):217-228.
  • Cooke MS, Olinski R, Evans MD. Does measurement of oxidative damege to DNA have clinical significance? Clin Chim Acta 2006; 365(1-2):30-49.
  • Cross CE. The spectrum of diseases (pp. 531–533). In: Cross, C.E. (moderator), Oxygen Radicals and Human Disease. Ann Intern Med 1987; 107:526–545.
  • Çetin S, Yur F, Taşpınar M, Yüksek V (2017). The Effect Of Some Minerals On Apotosis And DNA Damage İn Sodium Fluoride (Naf) Administered Renal And Osteoblast Cell Lines Fluoride (accepted)
  • Erel O (2005) .A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38 (12): 1103-1111.
  • Fattah EAA, Shaban SF, Ahmed FE (2010). The possible protective role of vitamin E on fluoride induced neurotoxicity in the frontal cortex of adult male albino rats. Egypt J Histol 2010; 33(1):103-113.
  • Ghiselli A, Serafini M, Natella F, Scaccini C.Total antioxidant capacity as a toll to assessredox status:criticalview and experimental data. Free Radic Biol Med 2000; 29(11):1106-1114.
  • Gregus Z, Fekete T, Halaszi E, Klaassen CD. Lipoic acid impairs glycine conjugation of banzoic acid and rena lexcretion of benzoylglycine. Drug Metab Disp 1996; 24:682–688.
  • Guney M, Oral B, Demirin H, Karahan N, Mungan T, Delibas N. Protective effects of vitamins C and E against endometrial damage and oxidative stress in fluoride intoxication. Clin Exp Pharmacol Physiol 2007; 34(5-6): 467-74.
  • Gutiérrez-Salinas J, García-Ortíz L, Morales González JA, at al. Invitro effect of sodium fluoride on malondialdehyde concentration and on superoxide dismutase, catalase, and glutathione peroxidase in human erythrocytes. The Scientific World Journal 2013; 2013:1-7.
  • Halliwell B, Gutteridge JMC. Oxygen free radical sandiron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys 1986; 246:501–514.
  • Kumari DS, Rao PR. Red cell membrane alterations in human chronic fluoride toxicity.Biochem Int 1991; 23(4):639-648 Lartillot S, Kedziora P, Athias A. Purification and characterization of a new fungal catalase. Prep Biochem 1988; 18(3): 241-246.
  • Ledwozyw A, Michalak J, Stepień A, Kadziołka A (1986). The relationship between plasma triglycerides, cholesterol, total lipids andl ipid peroxidation products during human atherosclerosis. Clin Chim Acta, 155, 3, 275-83.
  • Lee JH, Jung JY, Jeong YJ, at al. Involvement of both mitochondrial- anddeathreceptor-dependent apoptotic pathways regulatedby Bcl-2 family in sodium fluoride-induced apoptosis of the human gingival fibroblasts. Toxicology 2008; 243(3): 340-347.
  • Mc-Cord, JM, Keele BB, Fridovich I. An enzyme based the ory of obligate anaerobiosis, the physiological functions of superoxide dismutase. Proc Natl Acad Sci 1984; 68:1024–1027.
  • Mullenix PJ, Denbesten PK, Schunior A, Kernan WJ. Neurotoxicity of sodium fluoride in rats. Neurotoxicol Teratol 1995; 17:169-77.
  • Nicotera, P, Orrenius S. Role of thiols in protection against biological reactive intermediates. Adv Exp Med Biol 1986; 197, 41– 49.
  • Perumal E, Paul V, Govindarajan V, Panneerselvam L. Abriefreview on experimental fluorosis. Toxicol Lett 2013; 223(2):236-251.
  • Shanthakumari D, Srinivasalu S, Subramanian S. Effect of fluoride intoxication on lipid peroxidation and antioxidant status in experimental rats. Toxicology 2004; 204(2-3): 219-228.
  • Sharma A, Chinoy NJ. Role of freeradicals in fluoride induced toxicity in liver and kidney of mice and its reversal. Fluoride 1998; 31, 26.
  • Sies H. Damage to plasmid DNA by singlet oxygen and its protection. Mut Res 1993; 299: 183–191.
  • Singh M. Biochemical and Cytochemical alterations in liver and kidney following experimental fluorosis. Fluoride 1984; 17:81-93.
  • Soni MG, Kachole MS, Pawar SS. Alterations in drug metabolising enzymes and lipid peroxidation in different rat tissues by fluoride. Toxicol Lett 1984; 21: 167-172.
  • Vani ML, Reddy KP. Efects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluoride 2000; 33: 17–27.
  • Vani ML, Reddy KP. Effects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluoride 2000; 33:17-26
  • Yang Y, Lin X, Huang H, Feng D, Ba Y, Cheng X, Cui L. Sodium fluoride induces apoptosis throughre active oxygenspecies-mediated endoplasmic reticulum stres pathway in Sertoli cells. J Environ Sci 2015; 30: 81-89
  • Yur F, Mert N, Dede S, Değer Y, Ertekin A, Mert H, Yaşar S, Doğan İ,Işık A. Evaluation of serum lipoprotein and tissue antioxidant levels in sheep with fluorosis. Fluoride 2013; 46 (2):90-96.
  • Yüksek V, Dede S, Taşpınar M, Çetin S. The Effects Of Certain Vitamins On Apoptosis and DNA Damage in Sodium Fluoride (Naf) Administered Renal And Osteoblast Cell Lines Fluoride 2017 (accepted)
  • Zhang Y, Li W, Chi HS, Chen J, DenBesten PK. JNK/c-Junsignaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro. MatrixBiol 200; 26(8): 633–641.
  • Zhi-Zhong G, Pei-Si Y, Nai-den Y, Zong-Jie Z. An experimental study of blood biochemical diagnostic indices for chronic fluorosis. Fluoride 1989; 22: 112–118.

Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19)

Year 2017, Volume: 1 Issue: 1, 1 - 6, 01.12.2017

Abstract



Objective: This study was planned to determine the effect of certain
vitamin applications on antioxidant and oxidant parameters in the osteoblast
cell line exposed to sodium fluoride
in
vitro
and to evaluate the protective role of certain vitamins against
possible toxic effects of fluoride.




Materials
and Methods:
Cells were replicated in vitro conditions with regular
passaging 2-3 times weekly. MTF viability test was used to determine IC
50 of
NaF (5000μM) and proliferative doses of vitamins (Vitamin A: 10μM, Vitamin D:
10μM, Vitamin E: 60μM, Vitamin C: 100μM) for hFOB 1.19 cells. Cells were sown
in flasks as so to be 10
6. The study groups were identified as
control, NaF, vitamins and NaF+vitamins. After incubation for 24 hours, cells
treated with trypsin were prepared by freeze/thaw method and MTT viability
test, TAS, SOD, GSH, CAT, TOS and MDA analyzes were performed on these samples.




Results: In the hFOB 1.19 cell line, TAS levels decreased
significantly in the NaF group (p≤0.05), but were close to the control group in
NaF+vitamin groups with the exception of vitamin C. However, there was no
difference between the groups in terms of GSH level and CAT and SOD activities
when the control and NaF groups were compared. It was observed that TOS level
increased significantly in the NaF group (p<0.05), decreased in the
NaF+vitamin groups and were lower in the NaF+vitamin C and E groups than the
control group (p <0.05). While OSI was the highest in the NaF group, no
significant difference in MDA level was observed compared with the control
group.




Conclusion: As a result, it was found that NaF administration in the
osteoblast cell line increased oxidative stress and decreased following vitamin
application. It was found that the effect of NaF administration in the
osteoblast cell line on cell viability was consistent with the oxidative
stability and that the vitamin application conformably changed cell viability
and oxidative balance.

References

  • Altıntaş A, Fidancı UR, Sel T, Duru Ö, Başsatan A. Doğal ve Endüstriyel Florozisli Koyunlarda Böbrek Fonksiyonu ve Serum Protein Elektroforezi. Ankara Ü Vet Fak Derg 2000; 47:105-114.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J LabClinMed 1963; 61:882-90. Chance B, GreenStein DS, Roughton RJW. The mechanism of catalyse action steady state analysis. Arch Biochem Biophys 1982; 37:301–339.
  • Chaverri JP, Sánchez-Lozada LG, Osorio-Alonso H, Tapia E, Scholze A. New pathogenic concepts and the rapeutic approaches to oxidative stress in chronic kidney disease. Oxidative Medicine and Cellular Longevity 2016; 2016:1-21. Chlubek D,Poland S. Fluoride and oxidatif stress. Fluoride 2003;36(4):217-228.
  • Cooke MS, Olinski R, Evans MD. Does measurement of oxidative damege to DNA have clinical significance? Clin Chim Acta 2006; 365(1-2):30-49.
  • Cross CE. The spectrum of diseases (pp. 531–533). In: Cross, C.E. (moderator), Oxygen Radicals and Human Disease. Ann Intern Med 1987; 107:526–545.
  • Çetin S, Yur F, Taşpınar M, Yüksek V (2017). The Effect Of Some Minerals On Apotosis And DNA Damage İn Sodium Fluoride (Naf) Administered Renal And Osteoblast Cell Lines Fluoride (accepted)
  • Erel O (2005) .A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38 (12): 1103-1111.
  • Fattah EAA, Shaban SF, Ahmed FE (2010). The possible protective role of vitamin E on fluoride induced neurotoxicity in the frontal cortex of adult male albino rats. Egypt J Histol 2010; 33(1):103-113.
  • Ghiselli A, Serafini M, Natella F, Scaccini C.Total antioxidant capacity as a toll to assessredox status:criticalview and experimental data. Free Radic Biol Med 2000; 29(11):1106-1114.
  • Gregus Z, Fekete T, Halaszi E, Klaassen CD. Lipoic acid impairs glycine conjugation of banzoic acid and rena lexcretion of benzoylglycine. Drug Metab Disp 1996; 24:682–688.
  • Guney M, Oral B, Demirin H, Karahan N, Mungan T, Delibas N. Protective effects of vitamins C and E against endometrial damage and oxidative stress in fluoride intoxication. Clin Exp Pharmacol Physiol 2007; 34(5-6): 467-74.
  • Gutiérrez-Salinas J, García-Ortíz L, Morales González JA, at al. Invitro effect of sodium fluoride on malondialdehyde concentration and on superoxide dismutase, catalase, and glutathione peroxidase in human erythrocytes. The Scientific World Journal 2013; 2013:1-7.
  • Halliwell B, Gutteridge JMC. Oxygen free radical sandiron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys 1986; 246:501–514.
  • Kumari DS, Rao PR. Red cell membrane alterations in human chronic fluoride toxicity.Biochem Int 1991; 23(4):639-648 Lartillot S, Kedziora P, Athias A. Purification and characterization of a new fungal catalase. Prep Biochem 1988; 18(3): 241-246.
  • Ledwozyw A, Michalak J, Stepień A, Kadziołka A (1986). The relationship between plasma triglycerides, cholesterol, total lipids andl ipid peroxidation products during human atherosclerosis. Clin Chim Acta, 155, 3, 275-83.
  • Lee JH, Jung JY, Jeong YJ, at al. Involvement of both mitochondrial- anddeathreceptor-dependent apoptotic pathways regulatedby Bcl-2 family in sodium fluoride-induced apoptosis of the human gingival fibroblasts. Toxicology 2008; 243(3): 340-347.
  • Mc-Cord, JM, Keele BB, Fridovich I. An enzyme based the ory of obligate anaerobiosis, the physiological functions of superoxide dismutase. Proc Natl Acad Sci 1984; 68:1024–1027.
  • Mullenix PJ, Denbesten PK, Schunior A, Kernan WJ. Neurotoxicity of sodium fluoride in rats. Neurotoxicol Teratol 1995; 17:169-77.
  • Nicotera, P, Orrenius S. Role of thiols in protection against biological reactive intermediates. Adv Exp Med Biol 1986; 197, 41– 49.
  • Perumal E, Paul V, Govindarajan V, Panneerselvam L. Abriefreview on experimental fluorosis. Toxicol Lett 2013; 223(2):236-251.
  • Shanthakumari D, Srinivasalu S, Subramanian S. Effect of fluoride intoxication on lipid peroxidation and antioxidant status in experimental rats. Toxicology 2004; 204(2-3): 219-228.
  • Sharma A, Chinoy NJ. Role of freeradicals in fluoride induced toxicity in liver and kidney of mice and its reversal. Fluoride 1998; 31, 26.
  • Sies H. Damage to plasmid DNA by singlet oxygen and its protection. Mut Res 1993; 299: 183–191.
  • Singh M. Biochemical and Cytochemical alterations in liver and kidney following experimental fluorosis. Fluoride 1984; 17:81-93.
  • Soni MG, Kachole MS, Pawar SS. Alterations in drug metabolising enzymes and lipid peroxidation in different rat tissues by fluoride. Toxicol Lett 1984; 21: 167-172.
  • Vani ML, Reddy KP. Efects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluoride 2000; 33: 17–27.
  • Vani ML, Reddy KP. Effects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluoride 2000; 33:17-26
  • Yang Y, Lin X, Huang H, Feng D, Ba Y, Cheng X, Cui L. Sodium fluoride induces apoptosis throughre active oxygenspecies-mediated endoplasmic reticulum stres pathway in Sertoli cells. J Environ Sci 2015; 30: 81-89
  • Yur F, Mert N, Dede S, Değer Y, Ertekin A, Mert H, Yaşar S, Doğan İ,Işık A. Evaluation of serum lipoprotein and tissue antioxidant levels in sheep with fluorosis. Fluoride 2013; 46 (2):90-96.
  • Yüksek V, Dede S, Taşpınar M, Çetin S. The Effects Of Certain Vitamins On Apoptosis and DNA Damage in Sodium Fluoride (Naf) Administered Renal And Osteoblast Cell Lines Fluoride 2017 (accepted)
  • Zhang Y, Li W, Chi HS, Chen J, DenBesten PK. JNK/c-Junsignaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro. MatrixBiol 200; 26(8): 633–641.
  • Zhi-Zhong G, Pei-Si Y, Nai-den Y, Zong-Jie Z. An experimental study of blood biochemical diagnostic indices for chronic fluorosis. Fluoride 1989; 22: 112–118.
There are 32 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section 2017 Volume 1 Number 1
Authors

Veysel Yuksek 0000-0001-7432-4989

Sedat Cetın

Ayse Usta

Ahmet Ufuk Komuroglu

Semiha Dede

Publication Date December 1, 2017
Submission Date November 21, 2017
Published in Issue Year 2017 Volume: 1 Issue: 1

Cite

APA Yuksek, V., Cetın, S., Usta, A., Komuroglu, A. U., et al. (2017). Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19). Turkish Journal of Veterinary Research, 1(1), 1-6.
AMA Yuksek V, Cetın S, Usta A, Komuroglu AU, Dede S. Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19). TJVR. December 2017;1(1):1-6.
Chicago Yuksek, Veysel, Sedat Cetın, Ayse Usta, Ahmet Ufuk Komuroglu, and Semiha Dede. “Effect of Some Vitamins on antioxidant/Prooxidant Parameters in Sodium Fluoride (NaF)-Treated Cell Line (hFOB 1.19)”. Turkish Journal of Veterinary Research 1, no. 1 (December 2017): 1-6.
EndNote Yuksek V, Cetın S, Usta A, Komuroglu AU, Dede S (December 1, 2017) Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19). Turkish Journal of Veterinary Research 1 1 1–6.
IEEE V. Yuksek, S. Cetın, A. Usta, A. U. Komuroglu, and S. Dede, “Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19)”, TJVR, vol. 1, no. 1, pp. 1–6, 2017.
ISNAD Yuksek, Veysel et al. “Effect of Some Vitamins on antioxidant/Prooxidant Parameters in Sodium Fluoride (NaF)-Treated Cell Line (hFOB 1.19)”. Turkish Journal of Veterinary Research 1/1 (December 2017), 1-6.
JAMA Yuksek V, Cetın S, Usta A, Komuroglu AU, Dede S. Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19). TJVR. 2017;1:1–6.
MLA Yuksek, Veysel et al. “Effect of Some Vitamins on antioxidant/Prooxidant Parameters in Sodium Fluoride (NaF)-Treated Cell Line (hFOB 1.19)”. Turkish Journal of Veterinary Research, vol. 1, no. 1, 2017, pp. 1-6.
Vancouver Yuksek V, Cetın S, Usta A, Komuroglu AU, Dede S. Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19). TJVR. 2017;1(1):1-6.