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Year 2024, Volume: 8 Issue: 1, 63 - 69, 01.04.2024

Osman Bulut Tolga Meriç Dümbek Ali Sorucu Zehra Avcı

https://doi.org/10.47748/tjvr.1387484

Abstract

Project Number

Yok

References

  • Abd Rashid N, Mohammed SNF, Syed Abd Halim SA, Abd Ghafar N, Abdul Jalil NA. Therapeutic potential of honey and propolis on ocular disease. Pharmaceuticals. 2022; 15:1419.
  • Alfaris AA, Abdulsamad RK, Swad AA. Comparative studies between propolis, dexametason and gentamycin treatments of induced corneal ulcer in rabbits. Iraqi J Vet Sci. 2009; 23:75-80.
  • Alihosseini F. Plant-based compounds for antimicrobial textiles. In: Sun G, eds. Antimicrobial Textiles. 1st ed. Duxford: Elsevier; 2016. p.155-195.
  • Alkan M, Alkan H, Albayrak O, Onel A. Çam, vişne ve kayısı reçinelerinin antibakteriyal özelliklerinin incelenmesi. Caucasian Journal of Science. 2016; 3:52-57.
  • Angin N, Ertas M. Farklı çözücü türlerinin ekstraksiyon reçinesinin verimi ve kimyasal özellikleri üzerine etkisi. Turkish Journal of Forestry. 2021; 439-443.
  • Barnes TM, Greive KA. Topical pine tar: History, properties and use as a treatment for common skin conditions. Aust J Dermatol. 2017; 58:80-85.
  • Beltran Sanchidrian V. Vibrational spectroscopies study of Pinus resin in materials from cultural heritage objects [Doctor of Philosophy]. Catalunya: Universitat Politècnica de Catalunya; 2016.
  • Boudjelal A, Napoli E, Benkhaled A, et al. In vivo wound healing effect of Italian and Algerian Pistacia vera L. resins. Fitoterapia. 2022; 159:105197.
  • Bulut O, Sorucu A, Dumbek TM, Avci Z. Effects of propolis-containing nanofibers on corneal wound in rats. J Vet Bio Sci. 2023; 8(3):183-190.
  • Chandler HL, Tan T, Yang C, et al. MG53 promotes corneal wound healing and mitigates fibrotic remodeling in rodents. Commun Biol. 2019; 2:71.
  • Dell B, McComb AJ. Plant resins-Their Formation, secretion and possible functions. In: Woolhouse HW, eds. 1st ed. Leeds: Academic Press; 1979. p.277-316.
  • Demir A, Erdikmen DO, Sevim ZT, Altundag Y. The use of an autologous platelet-rich fibrin (PRF) membrane for the treatment of deep corneal ulcers in dogs. Vet Arh. 2022; 92(4):443-458.
  • Fernandes-Cunha GM, Na K-S, Putra I, et al. Corneal wound healing effects of mesenchymal stem cell secretome delivered within a viscoelastic gel carrier. Stem Cells Transl Med. 2019; 8:478-489.
  • Güzel C. Kızılçam (pinus brutia ten.)’da yüksekliğe bağlı terpen profillerinin varyasyonu [Yüksek Lisans Tezi]. Denizli: Fen Bilimleri Enstitüsü; 2019.
  • Ho W, Chiang T, Chang S, Chen YH, Hu FR, Wang IJ. Enhanced corneal wound healing with hyaluronic acid and high‐potassium artificial tears. Clin Exp Optom. 2013; 96:536-541.
  • Hu Y, Shi H, Ma X, et al. Highly stable fibronectin-mimetic-peptide-based supramolecular hydrogel to accelerate corneal wound healing. Acta Biomater. 2023; 159:128-139.
  • Khmelnitskii OK, Simbirtsev VG, Konusova VG, et al. Pine resin and biopin ointment: effects on cell composition and histochemical changes in wounds. Bull Exp Biol Med. 2002; 133:672-674.
  • Kibar Kurt B, Belge A. Investigation of the effectiveness of dehydrated corneal collagen barriers on corneal defects: An experimental rabbit model. Ankara Üniv Vet Fak Derg. 2021; 68:147-154.
  • Lin CB, Boehnke M. Effect of fortified antibiotic solutions on corneal epithelial wound healing. Cornea. 2000; 19(2):204-206
  • Martin LFT, Rocha EM, Garcia SB, Paula JS. Topical Brazilian propolis improves corneal wound healing and inflammation in rats following alkali burns. BMC Complement Altern Med. 2013; 13:337.
  • McDonald TO, Borgmann AR, Roberts MD, Fox LG. Corneal wound healing. Invest Ophthalmol. 1970; 703-709.
  • Nagai N, Murao T, Ito Y, Okamoto N, Sasaki M. Enhancing effects of sericin on corneal wound healing in rat debrided corneal epithelium. Biol Pharm Bull. 2009; 32:933-936.
  • Nagai N, Murao T, Okamoto N, Ito Y. Comparison of corneal wound healing rates after ınstillation of commercially available latanoprost and travoprost in rat debrided corneal epithelium. J Oleo Sci. 2010; 59:135-141.
  • Ozturk F, Kurt E, Inan UU, Emiroglu L, Ilker SS. The effects of acetylcholine and propolis extract on corneal epithelial wound healing in rats. Cornea. 1999; 18:466-471.
  • Park JY, Lee YK, Lee D-S, et al. Abietic acid isolated from pine resin (Resina Pini) enhances angiogenesis in HUVECs and accelerates cutaneous wound healing in mice. J Ethnopharmacol. 2017; 203:279-287.
  • Pérez-Recalde M, Ruiz Arias IE, Hermida ÉB. Could essential oils enhance biopolymers performance for wound healing? A systematic review. Phytomedicine. 2018; 38:57-65.
  • Peyman GA, Kivilcim M, Morales AM, DellaCroce JT, Conway MD. Inhibition of corneal angiogenesis by ascorbic acid in the rat model. Graefes Arch Clin Exp Ophthalmol. 2007; 245:1461-1467.
  • Rodrigues‐Corrêa KC da S, Lima JC, Fett‐Neto AG. Pine oleoresin: tapping green chemicals, biofuels, food protection, and carbon sequestration from multipurpose trees. Food Energy Secur. 2012; 1:81-93.
  • Romo-Rico J, Krishna SM, Bazaka K, Golledge J, Jacob MV. Potential of plant secondary metabolite-based polymers to enhance wound healing. Acta Biomater. 2022; 147:34-49.
  • Rozbahani A, Moghtadaiee E, Norbakhsh M. Histomorphometric Study on the Effect of Pine Tree Gum on Wound Healing of Wistar Rats. J. Med Herb. 2019; 9:99–106.
  • Salatino A, Fernandes-Silva CC, Righi AA, Luiza M, Salatino F. Propolis research and the chemistry of plant products. Nat Prod Rep. 2011; 28:925.
  • Shah JB. The history of wound care. J Am Col Certif Wound Spec. 2011; 3:65-66.
  • Sharma A, Sharma L, Goyal R. A Review on himalayan pine species: ethnopharmacological, phytochemical and pharmacological aspects. Pharmacognosy Journal. 2018; 10:611-619.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, Fidarov EV, Paramonov BA, Chebotarev VY. Pine resin and biopin ointment: effects on free radical processes. Bull Exp Biol Med. 2002a; 133:265-267.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, et al. Pine resin and biopin ointment: immunotoxic and allergenic activity. Bull Exp Biol Med. 2002b; 133:384-385.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, et al. Pine resin and Biopin ointment: effects on nonspecific resistance of organisms. Bull Exp Biol Med. 2002c; 133:141-143.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, et al. Pine resin and biopin ointment: effects of water-soluble fractions on functional activity of peripheral blood neutrophils. Bull Exp Biol Med. 2002d; 134:50-53.
  • Simbirtsev AS, Konusova VG, McHelidze EZ, et al. Pine resin and Biopin ointment: effects on repair processes in tissues. Bull Exp Biol Med. 2002e; 133:457-460.
  • Simbirtsev AS, Konusuva VG, Mchedlidze GS, et al. Effect of pine resin and Biopin ointment on T and B cell immunity. Bull Exp Biol Med. 2002f; 133:144-147.
  • Sinmez C, Aslim G, Yasar A. An ethnoveterinary study on plants used in the treatment of dermatological diseases in Central Anatolia, Turkey. J Complement Med Res. 2018; 9:71.
  • Venkata ALK, Sivaram S, Sajeet M, Sanjay PM, Srilakshman G, Sundaram MM. Review on terpenoid mediated nanoparticles: significance, mechanism, and biomedical applications. ANSN. 2022; 13:033003.
  • Zagon IS, Sassani JW, McLaughlin PJ. Cellular dynamics of corneal wound re-epithelialization in the rat. I. Fate of ocular surface epithelial cells synthesizing DNA prior to wounding. Brain Res. 2000; 882:149-163.
  • Zagon IS. Use of topical ınsulin to normalize corneal epithelial healing in diabetes mellitus. Arch Ophthalmol. 2007; 125:1082-1088.

Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats

Year 2024, Volume: 8 Issue: 1, 63 - 69, 01.04.2024

Osman Bulut Tolga Meriç Dümbek Ali Sorucu Zehra Avcı

https://doi.org/10.47748/tjvr.1387484

Abstract

Pine resin is a product obtained from plants belonging to the Pinaceae family and traditionally used in the treatment of wounds. The aim of this study is to determine the effectiveness of pine resin in corneal wounds. In this study, three groups of 7 male Wistar Albino rats (n=7), each 2 months old, were established. To create the corneal wound model, the rats were anesthetized and the borders of the wound to be created on the corneal surface were determined using a 3 mm punch biopsy, then the first two layers of the cornea were removed with a corneal knife. Then, the first group was considered as the control group and no treatment was performed. The second group was determined as the pine resin group and applied once a day. The third group was considered as the drug group and was administered once a day. Fluorescein staining was performed every day for three days and the results were recorded. Pine resin group showed the fastest recovery. On the third day, the rats were euthanized, and their eyes were enucleated. The collected eyes were sent for histopathologic examination and stained with hematoxylin-eosin. The lesions in the examined specimens were evaluated under microscope for hyperemia, vascularization, cellular infiltration, and corneal edema. As a result of the study, ulceration was observed in the pine resin group. The study concluded that pine resin reduces clinical symptoms and promotes healing in corneal wounds.

Keywords

Fluorescein Natural Rosin Terpenoids Ulceration

Ethical Statement

Mugla Sitki Kocman University Animal Experiments Local Ethics Committee under permit number 24.08.23/20-23

Project Number

Yok

References

  • Abd Rashid N, Mohammed SNF, Syed Abd Halim SA, Abd Ghafar N, Abdul Jalil NA. Therapeutic potential of honey and propolis on ocular disease. Pharmaceuticals. 2022; 15:1419.
  • Alfaris AA, Abdulsamad RK, Swad AA. Comparative studies between propolis, dexametason and gentamycin treatments of induced corneal ulcer in rabbits. Iraqi J Vet Sci. 2009; 23:75-80.
  • Alihosseini F. Plant-based compounds for antimicrobial textiles. In: Sun G, eds. Antimicrobial Textiles. 1st ed. Duxford: Elsevier; 2016. p.155-195.
  • Alkan M, Alkan H, Albayrak O, Onel A. Çam, vişne ve kayısı reçinelerinin antibakteriyal özelliklerinin incelenmesi. Caucasian Journal of Science. 2016; 3:52-57.
  • Angin N, Ertas M. Farklı çözücü türlerinin ekstraksiyon reçinesinin verimi ve kimyasal özellikleri üzerine etkisi. Turkish Journal of Forestry. 2021; 439-443.
  • Barnes TM, Greive KA. Topical pine tar: History, properties and use as a treatment for common skin conditions. Aust J Dermatol. 2017; 58:80-85.
  • Beltran Sanchidrian V. Vibrational spectroscopies study of Pinus resin in materials from cultural heritage objects [Doctor of Philosophy]. Catalunya: Universitat Politècnica de Catalunya; 2016.
  • Boudjelal A, Napoli E, Benkhaled A, et al. In vivo wound healing effect of Italian and Algerian Pistacia vera L. resins. Fitoterapia. 2022; 159:105197.
  • Bulut O, Sorucu A, Dumbek TM, Avci Z. Effects of propolis-containing nanofibers on corneal wound in rats. J Vet Bio Sci. 2023; 8(3):183-190.
  • Chandler HL, Tan T, Yang C, et al. MG53 promotes corneal wound healing and mitigates fibrotic remodeling in rodents. Commun Biol. 2019; 2:71.
  • Dell B, McComb AJ. Plant resins-Their Formation, secretion and possible functions. In: Woolhouse HW, eds. 1st ed. Leeds: Academic Press; 1979. p.277-316.
  • Demir A, Erdikmen DO, Sevim ZT, Altundag Y. The use of an autologous platelet-rich fibrin (PRF) membrane for the treatment of deep corneal ulcers in dogs. Vet Arh. 2022; 92(4):443-458.
  • Fernandes-Cunha GM, Na K-S, Putra I, et al. Corneal wound healing effects of mesenchymal stem cell secretome delivered within a viscoelastic gel carrier. Stem Cells Transl Med. 2019; 8:478-489.
  • Güzel C. Kızılçam (pinus brutia ten.)’da yüksekliğe bağlı terpen profillerinin varyasyonu [Yüksek Lisans Tezi]. Denizli: Fen Bilimleri Enstitüsü; 2019.
  • Ho W, Chiang T, Chang S, Chen YH, Hu FR, Wang IJ. Enhanced corneal wound healing with hyaluronic acid and high‐potassium artificial tears. Clin Exp Optom. 2013; 96:536-541.
  • Hu Y, Shi H, Ma X, et al. Highly stable fibronectin-mimetic-peptide-based supramolecular hydrogel to accelerate corneal wound healing. Acta Biomater. 2023; 159:128-139.
  • Khmelnitskii OK, Simbirtsev VG, Konusova VG, et al. Pine resin and biopin ointment: effects on cell composition and histochemical changes in wounds. Bull Exp Biol Med. 2002; 133:672-674.
  • Kibar Kurt B, Belge A. Investigation of the effectiveness of dehydrated corneal collagen barriers on corneal defects: An experimental rabbit model. Ankara Üniv Vet Fak Derg. 2021; 68:147-154.
  • Lin CB, Boehnke M. Effect of fortified antibiotic solutions on corneal epithelial wound healing. Cornea. 2000; 19(2):204-206
  • Martin LFT, Rocha EM, Garcia SB, Paula JS. Topical Brazilian propolis improves corneal wound healing and inflammation in rats following alkali burns. BMC Complement Altern Med. 2013; 13:337.
  • McDonald TO, Borgmann AR, Roberts MD, Fox LG. Corneal wound healing. Invest Ophthalmol. 1970; 703-709.
  • Nagai N, Murao T, Ito Y, Okamoto N, Sasaki M. Enhancing effects of sericin on corneal wound healing in rat debrided corneal epithelium. Biol Pharm Bull. 2009; 32:933-936.
  • Nagai N, Murao T, Okamoto N, Ito Y. Comparison of corneal wound healing rates after ınstillation of commercially available latanoprost and travoprost in rat debrided corneal epithelium. J Oleo Sci. 2010; 59:135-141.
  • Ozturk F, Kurt E, Inan UU, Emiroglu L, Ilker SS. The effects of acetylcholine and propolis extract on corneal epithelial wound healing in rats. Cornea. 1999; 18:466-471.
  • Park JY, Lee YK, Lee D-S, et al. Abietic acid isolated from pine resin (Resina Pini) enhances angiogenesis in HUVECs and accelerates cutaneous wound healing in mice. J Ethnopharmacol. 2017; 203:279-287.
  • Pérez-Recalde M, Ruiz Arias IE, Hermida ÉB. Could essential oils enhance biopolymers performance for wound healing? A systematic review. Phytomedicine. 2018; 38:57-65.
  • Peyman GA, Kivilcim M, Morales AM, DellaCroce JT, Conway MD. Inhibition of corneal angiogenesis by ascorbic acid in the rat model. Graefes Arch Clin Exp Ophthalmol. 2007; 245:1461-1467.
  • Rodrigues‐Corrêa KC da S, Lima JC, Fett‐Neto AG. Pine oleoresin: tapping green chemicals, biofuels, food protection, and carbon sequestration from multipurpose trees. Food Energy Secur. 2012; 1:81-93.
  • Romo-Rico J, Krishna SM, Bazaka K, Golledge J, Jacob MV. Potential of plant secondary metabolite-based polymers to enhance wound healing. Acta Biomater. 2022; 147:34-49.
  • Rozbahani A, Moghtadaiee E, Norbakhsh M. Histomorphometric Study on the Effect of Pine Tree Gum on Wound Healing of Wistar Rats. J. Med Herb. 2019; 9:99–106.
  • Salatino A, Fernandes-Silva CC, Righi AA, Luiza M, Salatino F. Propolis research and the chemistry of plant products. Nat Prod Rep. 2011; 28:925.
  • Shah JB. The history of wound care. J Am Col Certif Wound Spec. 2011; 3:65-66.
  • Sharma A, Sharma L, Goyal R. A Review on himalayan pine species: ethnopharmacological, phytochemical and pharmacological aspects. Pharmacognosy Journal. 2018; 10:611-619.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, Fidarov EV, Paramonov BA, Chebotarev VY. Pine resin and biopin ointment: effects on free radical processes. Bull Exp Biol Med. 2002a; 133:265-267.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, et al. Pine resin and biopin ointment: immunotoxic and allergenic activity. Bull Exp Biol Med. 2002b; 133:384-385.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, et al. Pine resin and Biopin ointment: effects on nonspecific resistance of organisms. Bull Exp Biol Med. 2002c; 133:141-143.
  • Simbirtsev AS, Konusova VG, McHedlidze GS, et al. Pine resin and biopin ointment: effects of water-soluble fractions on functional activity of peripheral blood neutrophils. Bull Exp Biol Med. 2002d; 134:50-53.
  • Simbirtsev AS, Konusova VG, McHelidze EZ, et al. Pine resin and Biopin ointment: effects on repair processes in tissues. Bull Exp Biol Med. 2002e; 133:457-460.
  • Simbirtsev AS, Konusuva VG, Mchedlidze GS, et al. Effect of pine resin and Biopin ointment on T and B cell immunity. Bull Exp Biol Med. 2002f; 133:144-147.
  • Sinmez C, Aslim G, Yasar A. An ethnoveterinary study on plants used in the treatment of dermatological diseases in Central Anatolia, Turkey. J Complement Med Res. 2018; 9:71.
  • Venkata ALK, Sivaram S, Sajeet M, Sanjay PM, Srilakshman G, Sundaram MM. Review on terpenoid mediated nanoparticles: significance, mechanism, and biomedical applications. ANSN. 2022; 13:033003.
  • Zagon IS, Sassani JW, McLaughlin PJ. Cellular dynamics of corneal wound re-epithelialization in the rat. I. Fate of ocular surface epithelial cells synthesizing DNA prior to wounding. Brain Res. 2000; 882:149-163.
  • Zagon IS. Use of topical ınsulin to normalize corneal epithelial healing in diabetes mellitus. Arch Ophthalmol. 2007; 125:1082-1088.
There are 43 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section 2024 Volume 8 Number 1
Authors

Osman Bulut 0000-0003-2773-8243

Tolga Meriç Dümbek 0000-0003-1734-3491

Ali Sorucu 0000-0002-0496-9498

Zehra Avcı 0000-0003-1853-4679

Project Number Yok
Early Pub Date April 2, 2024
Publication Date April 1, 2024
Submission Date November 7, 2023
Acceptance Date January 2, 2024
Published in Issue Year 2024 Volume: 8 Issue: 1

Cite

APA Bulut, O., Dümbek, T. M., Sorucu, A., Avcı, Z. (2024). Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats. Turkish Journal of Veterinary Research, 8(1), 63-69. https://doi.org/10.47748/tjvr.1387484
AMA Bulut O, Dümbek TM, Sorucu A, Avcı Z. Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats. TJVR. April 2024;8(1):63-69. doi:10.47748/tjvr.1387484
Chicago Bulut, Osman, Tolga Meriç Dümbek, Ali Sorucu, and Zehra Avcı. “Investigation of the Healing Effectiveness of Pine Resin in Experimentally Induced Corneal Wound in Rats”. Turkish Journal of Veterinary Research 8, no. 1 (April 2024): 63-69. https://doi.org/10.47748/tjvr.1387484.
EndNote Bulut O, Dümbek TM, Sorucu A, Avcı Z (April 1, 2024) Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats. Turkish Journal of Veterinary Research 8 1 63–69.
IEEE O. Bulut, T. M. Dümbek, A. Sorucu, and Z. Avcı, “Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats”, TJVR, vol. 8, no. 1, pp. 63–69, 2024, doi: 10.47748/tjvr.1387484.
ISNAD Bulut, Osman et al. “Investigation of the Healing Effectiveness of Pine Resin in Experimentally Induced Corneal Wound in Rats”. Turkish Journal of Veterinary Research 8/1 (April 2024), 63-69. https://doi.org/10.47748/tjvr.1387484.
JAMA Bulut O, Dümbek TM, Sorucu A, Avcı Z. Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats. TJVR. 2024;8:63–69.
MLA Bulut, Osman et al. “Investigation of the Healing Effectiveness of Pine Resin in Experimentally Induced Corneal Wound in Rats”. Turkish Journal of Veterinary Research, vol. 8, no. 1, 2024, pp. 63-69, doi:10.47748/tjvr.1387484.
Vancouver Bulut O, Dümbek TM, Sorucu A, Avcı Z. Investigation of the healing effectiveness of pine resin in experimentally induced corneal wound in rats. TJVR. 2024;8(1):63-9.
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