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Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls (Boz Irkı ve Holstein Melezi Erkek Sığırlarda IGF1 C472T ve TG C422T Polimorfizmlerinin Analizi)

Year 2018, Volume: 37 Issue: 1, 21 - 28, 01.06.2018
https://doi.org/10.30782/uluvfd.392081

Abstract

Bu çalışmanın
amacı, Boz ırkı ve Holstein melezi erkek sığırlarda insulin benzeri büyüme
faktörü 1 ve tiroglobulin genlerine ait genotipik dağılımın belirlenmesidir.
Çalışmada, akraba olmayan 30 baş Boz ırk ve 26 baş Holstein melez sığır
kullanılmıştır. Genomik DNA izolasyonu tam kandan standart fenol-kloroform
yöntemi ile yapılmıştır. İnsulin benzeri büyüme faktörü 1 ve tiroglobulin genleri,
C472T ve C422T polimorfizmlerine ait genotiplendirme işlemi için ise polimeraz
zincir reaksiyonu-
restriksiyon parçacık uzunluk polimorfizmi tekniği
kullanılmıştır. Hardy-Weinberg eşitliğine uyum PopGene paket programı ile hesaplanmıştır.
Bununla birlikte, heterozigotluk, efektif allel sayısı ve polimorfizm bilgi
içeriğinden oluşan populasyon genetiği parametreleri allelik dağılım temel
alınarak belirlenmiştir. İnsulin benzeri büyüme faktörü 1 geni için, A alleli frekansı
iki ırk grubunda da yüksek bulunmuş ve BB genotipi gözlenmemiştir. Tiroglobulin
geni için ise, TT genotipi gözlenmemiş, buna ek olarak, C allelinin ise hem Boz
ırkta hem de Holstein melezlerinde neredeyse sabit olduğu belirlenmiştir. Bunun
yanı sıra, oldukça düşük minör allel frekansları populasyon indekslerindeki
düşük varyasyonlara sebep olmuştur. Bu çalışmadan elde edilen sonuçların insulin
benzeri büyüme faktörü 1 ve tiroglobulin genleri kapsamında sığır genomunda
yapılacak olan sonraki çalışmalara yararlı olabileceği düşünülmektedir.  

References

  • Akis I, Oztabak K. Bos indicus associated alleles in Anatolian cattle breeds support zebu introgression into Near East. J of Biol Res-Thessaloniki 19: 131-138, 2013.
  • Akis I, Oztabak K, Gonulalp I, Mengi A, Un C. IGF-1 and IGF-1R gene polymorphisms in East Anatolian Red and South Anatolian Red cattle breeds. Russ J Genet, 46: 439-442, 2010.
  • Andrade P, Grossi D, Paz C, Alencar M, Regitano L, Munari D. Association of an insulin‐like growth factor 1 gene microsatellite with phenotypic variation and estimated breeding values of growth traits in Canchim cattle. Anim Genet, 39, 480-485, 2008.
  • Anton I, Kovacs K, Fesus L, Varhegyi J, Lehel L, Hajda Z, Polgar J, Szabo F, Zsolnai A. Effect of DGAT1 and TG gene polymorphisms on intramuscular fat and on milk production traits in different cattle breeds in Hungary. Acta Vet Hung, 56: 181-186, 2008.
  • Anton I, Kovacs K, Hollo G, Farkas V, Szabo F, Egerszegi I, Ratky J, Zsolnai A, Brussow KP. Effect of DGAT1, leptin and TG gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary. Arch Tierz, 4: 307-314, 2012.
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 32: 314-331, 1980.
  • Casas E, White S, Riley D, Smith T, Brenneman R, Olson T, Johnson D, Coleman S, Bennett G, Chase C. Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. J Anim Sci, 83: 13-19, 2005.
  • Choudhary V, Kumar P, Bhattacharya TK, Bhushan B, Sharma A. DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. Genet Mol Biol, 28: 740-742, 2005.
  • Curi RA, De Oliveira H, Silveira AC, Lopes C. Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest Prod Sci, 94: 159-167, 2005.
  • De la Rosa Reyna X, Montoya H, Castrellon V, Rincon A, Bracamonte M, Vera W. Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genet Mol Res 9: 875-883, 2010.
  • Falconer DS, Mackay TFC, Introduction to quantitative genetics, 4th Ed., Pearson Education Ltd, Harlow, England, 1996.
  • Gan QF, Zhang LP, Li JY, Hou GY, Gao X, Ren HY, Chen JB, Xu SZ. Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. J Appl Genet, 49: 251-255, 2008.
  • Ge W, Davis M, Hines H, Irvin K, Simmen R. Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. J Anim Sci; 79: 1757-1762, 2001.
  • Green MR, Sambrook J. Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol. In. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, pp. 47–48, 2012.
  • Grosse WM, Kappes SM, Laegreid WW, Keele JW, Chitko-McKown CG, Heaton MP. Single nucleotide polymorphism (SNP) discovery and linkage mapping of bovine cytokine genes. Mamm Genome, 10: 1062-1069, 1999.
  • Hayes B, Goddard ME. The distribution of the effects of genes affecting quantitative traits in livestock. Genet Sel Evol, 33: 209-229, 2001.
  • Hines H, Ge W, Zhao Q, Davis M. Association of genetic markers in growth hormone and insulin-like growth factor-I loci with lactation traits in Holsteins. Anim Genet, Supplement 29: 63-69, 1998.
  • Lacorte G, Machado M, Martinez M, Campos A, Maciel R, Verneque R, Teodoro R, Peixoto M, Carvalho M, Fonseca C. DGAT1 K232A polymorphism in Brazilian cattle breeds. Genet Mol Res, 5: 475-482, 2006.
  • Lande R, Thompson R. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics, 124: 743-756, 1990.
  • Le Hir H, Nott A, Moore MJ. How introns influence and enhance eukaryotic gene expression. Trends Biochem Sci, 28: 215-220, 2003.
  • Li C, Basarab J, Snelling W, Benkel B, Murdoch B, Hansen C, Moore S. Assessment of positional candidate genes myf 5 and igf 1 for growth on bovine chromosome 5 in commercial lines of Bos taurus 1. J Anim Sci, 82: 1-7, 2004.
  • Menezes MPC, Martinez AM, Ribeiro MN, Pimenta Filho EC, Bermejo JVD. Genetic characterization of Brazilian native breeds of goats using 27 markers microsatellites. Rev Bras Zootecn, 35: 1336-1341, 2006.
  • Moore S, Li C, Basarab J, Snelling W, Kneeland J, Murdoch B, Hansen C, Benkel B. Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus 1. J Anim Sci, 81: 1919-1925, 2003.
  • Nei M, Roychoudhury A. Sampling variances of heterozygosity and genetic distance. Genetics, 76: 379-390, 1974.
  • Pannier L, Mullen A, Hamill R, Stapleton P, Sweeney T. Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat Sci, 85: 515-518, 2010.
  • Rincker C, Pyatt N, Berger L, Faulkner D. Relationship among GeneSTAR marbling marker, intramuscular fat deposition, and expected progeny differences in early weaned Simmental steers. J Anim Sci, 84: 686-693, 2006.
  • Shin S, Chung E. Association of SNP marker in the thyroglobulin gene with carcass and meat quality traits in Korean cattle. Asian-Australas J Anim Sci, 20: 172-177, 2007.Siadkowska E, Zwierzchowski L, Oprzadek J, Strzalkowska N, Bagnicka E, Krzyzewski J. Effect of polymorphism in IGF-1 gene on production traits in Polish Holstein-Friesian cattle. Anim Sci Pap Rep, 24: 225-237, 2006.
  • Thaller G, Kühn C, Winter A, Ewald G, Bellmann O, Wegner J, Zühlke H, Fries R. DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Anim Genet, 34: 354-357, 2003.
  • Trakovicka A, Moravcikova N, Kasarda R. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochim Pol, 60: 783-787, 2013.
  • Upton Z, Yandell CA, Degger BG, Chan SJ, Moriyama S, Francis GL, Ballard FJ. Evolution of insulin-like growth factor-I (IGF-I) action: in vitro characterization of vertebrate IGF-I proteins. Comp Biochem Physiol B Biochem Mol Biol, 121: 35-41, 1998.
  • Yeh FC, Yang RC, Boyle TB, Ye Z, Mao JX, POPGENE: the user-friendly shareware for population genetic analysis, Molecular biology and biotechnology centre, University of Alberta, Canada, 2000.

Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls

Year 2018, Volume: 37 Issue: 1, 21 - 28, 01.06.2018
https://doi.org/10.30782/uluvfd.392081

Abstract

The aim of the study was to determine genotypic distribution of bovine
insulin-like growth factor 1 and thyroglobulin genes in Turkish Grey Steppe (Boz)
and Holstein crossbred bulls. In the study, unrelated 30 Turkish Grey and 26 Holstein
crossbred cattle were used. Genomic DNA was isolated from whole blood using
standart
phenol-chloroform
extraction method
. The polymerase chain reaction-restriction fragment length polymorphism
technique was used for genotyping of C472T and C422T polymorphisms in insulin-like
growth factor 1 and thyroglobulin genes, respectively. Hardy-Weinberg
equilibrium was calculated by using PopGene software program. In addition the
population indexes including heterozygosity, number of effective alleles and
polymorphism information content were determined on the basis of allelic
distribution. For insulin-like growth factor 1 gene, A allele frequency was
higher and BB genotype was not present in both breed groups. For thyroglobulin gene,
TT genotype was not present and moreover C allele was almost fixed in both
Turkish Grey and Holstein crossbred bulls. In addition, extremely low minor
allele frequencies in resulted in low genetic variabilities of population indexes.
The present results may be useful for future studies conducted on bovine genome
with respect to insulin-like growth factor 1 and thyroglobulin genes.    

References

  • Akis I, Oztabak K. Bos indicus associated alleles in Anatolian cattle breeds support zebu introgression into Near East. J of Biol Res-Thessaloniki 19: 131-138, 2013.
  • Akis I, Oztabak K, Gonulalp I, Mengi A, Un C. IGF-1 and IGF-1R gene polymorphisms in East Anatolian Red and South Anatolian Red cattle breeds. Russ J Genet, 46: 439-442, 2010.
  • Andrade P, Grossi D, Paz C, Alencar M, Regitano L, Munari D. Association of an insulin‐like growth factor 1 gene microsatellite with phenotypic variation and estimated breeding values of growth traits in Canchim cattle. Anim Genet, 39, 480-485, 2008.
  • Anton I, Kovacs K, Fesus L, Varhegyi J, Lehel L, Hajda Z, Polgar J, Szabo F, Zsolnai A. Effect of DGAT1 and TG gene polymorphisms on intramuscular fat and on milk production traits in different cattle breeds in Hungary. Acta Vet Hung, 56: 181-186, 2008.
  • Anton I, Kovacs K, Hollo G, Farkas V, Szabo F, Egerszegi I, Ratky J, Zsolnai A, Brussow KP. Effect of DGAT1, leptin and TG gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary. Arch Tierz, 4: 307-314, 2012.
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 32: 314-331, 1980.
  • Casas E, White S, Riley D, Smith T, Brenneman R, Olson T, Johnson D, Coleman S, Bennett G, Chase C. Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. J Anim Sci, 83: 13-19, 2005.
  • Choudhary V, Kumar P, Bhattacharya TK, Bhushan B, Sharma A. DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. Genet Mol Biol, 28: 740-742, 2005.
  • Curi RA, De Oliveira H, Silveira AC, Lopes C. Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest Prod Sci, 94: 159-167, 2005.
  • De la Rosa Reyna X, Montoya H, Castrellon V, Rincon A, Bracamonte M, Vera W. Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genet Mol Res 9: 875-883, 2010.
  • Falconer DS, Mackay TFC, Introduction to quantitative genetics, 4th Ed., Pearson Education Ltd, Harlow, England, 1996.
  • Gan QF, Zhang LP, Li JY, Hou GY, Gao X, Ren HY, Chen JB, Xu SZ. Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. J Appl Genet, 49: 251-255, 2008.
  • Ge W, Davis M, Hines H, Irvin K, Simmen R. Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. J Anim Sci; 79: 1757-1762, 2001.
  • Green MR, Sambrook J. Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol. In. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, pp. 47–48, 2012.
  • Grosse WM, Kappes SM, Laegreid WW, Keele JW, Chitko-McKown CG, Heaton MP. Single nucleotide polymorphism (SNP) discovery and linkage mapping of bovine cytokine genes. Mamm Genome, 10: 1062-1069, 1999.
  • Hayes B, Goddard ME. The distribution of the effects of genes affecting quantitative traits in livestock. Genet Sel Evol, 33: 209-229, 2001.
  • Hines H, Ge W, Zhao Q, Davis M. Association of genetic markers in growth hormone and insulin-like growth factor-I loci with lactation traits in Holsteins. Anim Genet, Supplement 29: 63-69, 1998.
  • Lacorte G, Machado M, Martinez M, Campos A, Maciel R, Verneque R, Teodoro R, Peixoto M, Carvalho M, Fonseca C. DGAT1 K232A polymorphism in Brazilian cattle breeds. Genet Mol Res, 5: 475-482, 2006.
  • Lande R, Thompson R. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics, 124: 743-756, 1990.
  • Le Hir H, Nott A, Moore MJ. How introns influence and enhance eukaryotic gene expression. Trends Biochem Sci, 28: 215-220, 2003.
  • Li C, Basarab J, Snelling W, Benkel B, Murdoch B, Hansen C, Moore S. Assessment of positional candidate genes myf 5 and igf 1 for growth on bovine chromosome 5 in commercial lines of Bos taurus 1. J Anim Sci, 82: 1-7, 2004.
  • Menezes MPC, Martinez AM, Ribeiro MN, Pimenta Filho EC, Bermejo JVD. Genetic characterization of Brazilian native breeds of goats using 27 markers microsatellites. Rev Bras Zootecn, 35: 1336-1341, 2006.
  • Moore S, Li C, Basarab J, Snelling W, Kneeland J, Murdoch B, Hansen C, Benkel B. Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus 1. J Anim Sci, 81: 1919-1925, 2003.
  • Nei M, Roychoudhury A. Sampling variances of heterozygosity and genetic distance. Genetics, 76: 379-390, 1974.
  • Pannier L, Mullen A, Hamill R, Stapleton P, Sweeney T. Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat Sci, 85: 515-518, 2010.
  • Rincker C, Pyatt N, Berger L, Faulkner D. Relationship among GeneSTAR marbling marker, intramuscular fat deposition, and expected progeny differences in early weaned Simmental steers. J Anim Sci, 84: 686-693, 2006.
  • Shin S, Chung E. Association of SNP marker in the thyroglobulin gene with carcass and meat quality traits in Korean cattle. Asian-Australas J Anim Sci, 20: 172-177, 2007.Siadkowska E, Zwierzchowski L, Oprzadek J, Strzalkowska N, Bagnicka E, Krzyzewski J. Effect of polymorphism in IGF-1 gene on production traits in Polish Holstein-Friesian cattle. Anim Sci Pap Rep, 24: 225-237, 2006.
  • Thaller G, Kühn C, Winter A, Ewald G, Bellmann O, Wegner J, Zühlke H, Fries R. DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Anim Genet, 34: 354-357, 2003.
  • Trakovicka A, Moravcikova N, Kasarda R. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochim Pol, 60: 783-787, 2013.
  • Upton Z, Yandell CA, Degger BG, Chan SJ, Moriyama S, Francis GL, Ballard FJ. Evolution of insulin-like growth factor-I (IGF-I) action: in vitro characterization of vertebrate IGF-I proteins. Comp Biochem Physiol B Biochem Mol Biol, 121: 35-41, 1998.
  • Yeh FC, Yang RC, Boyle TB, Ye Z, Mao JX, POPGENE: the user-friendly shareware for population genetic analysis, Molecular biology and biotechnology centre, University of Alberta, Canada, 2000.
There are 31 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Sena Ardicli

Publication Date June 1, 2018
Acceptance Date March 1, 2018
Published in Issue Year 2018 Volume: 37 Issue: 1

Cite

APA Ardicli, S. (2018). Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 37(1), 21-28. https://doi.org/10.30782/uluvfd.392081
AMA Ardicli S. Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. June 2018;37(1):21-28. doi:10.30782/uluvfd.392081
Chicago Ardicli, Sena. “Analysis of IGF1 C472T and TG C422T Polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 37, no. 1 (June 2018): 21-28. https://doi.org/10.30782/uluvfd.392081.
EndNote Ardicli S (June 1, 2018) Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 37 1 21–28.
IEEE S. Ardicli, “Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls”, Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol. 37, no. 1, pp. 21–28, 2018, doi: 10.30782/uluvfd.392081.
ISNAD Ardicli, Sena. “Analysis of IGF1 C472T and TG C422T Polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 37/1 (June 2018), 21-28. https://doi.org/10.30782/uluvfd.392081.
JAMA Ardicli S. Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2018;37:21–28.
MLA Ardicli, Sena. “Analysis of IGF1 C472T and TG C422T Polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol. 37, no. 1, 2018, pp. 21-28, doi:10.30782/uluvfd.392081.
Vancouver Ardicli S. Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2018;37(1):21-8.