Research Article
PAEP gene restriction fragment length polymorphism and its effects on milk composition in cross-bred Hamdani sheep
Abstract
β-lactoglobulin (βLG) stands as the primary whey protein in ruminant milk, synthesized by mammary gland cells during lactation and encoded by the βLG gene. This study aimed to assess the impact of βLG gene exon II polymorphism on milk composition traits in crossbreed Hamdani sheep. Sheep were examined for clinical diseases and mastitis. Milk and blood samples were only collected from healthy ewes. The composition and physical properties of milk were analyzed using milk autoanalyzer. The βLG gene exon II region's 452 bp PCR products were subjected to RFLP analysis using the RsaI restriction enzyme. Two genotypes, AA and AB, were identified for the βLG gene exon II region, with A and B allele frequencies of 0.7 and 0.3, respectively. Statistical analysis, conducted with Minitab® (Version: 19.2020.2.0), revealed that the AA genotype is associated with a higher milk fat percentage (p<0.05). However, no significant genotype effect was observed for other milk composition traits in crossbreed Hamdani sheep. These results suggest that ΒLG genotypes could serve as valuable indicators for enhancing milk composition in crossbreed Hamdani sheep through breeding programs.
Keywords
Ethical Statement
The study was approved by Siirt University Animal Experiments Local Ethics Committee (Approval no: 2023-01-10)
Supporting Institution
Siirt University
Project Number
2022-SIUVET-04
Thanks
The authors thank Assoc. Prof. Dr. Burçak ASLAN ÇELIK and Assoc. Prof. Dr. Behçet INAL for technical support in this study.
References
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- Baranyi, M., Kerekes, A., Hiripi, L., & Bősze, Z. (2010). Preliminary data on beta-lactoglobulin genetic polymorphisms in Hungarian Awassi and Racka sheep. Scientific Papers Animal Science and Biotechnologies, 43, 1-4.
- Barbano, D. M., Clark, J. L., Dunham, C. E., & Flemin, R. J. (1990). Kjeldahl method for determination of total nitrogen content of milk: collaborative study. Journal of the Association of Official Analytical Chemists, 73(6), 849-859. https://doi.org/10.1093/jaoac/73.6.849
- Bayraktar, M., & Shoshin, O. (2021). Determination effects of slc27a3 and β-lactoglobulin gene polymorphisms on the milk composition in Hamdani sheep. Applied Ecology and Environmental Research, 19(4), 3293-3302. http://dx.doi.org/10. 15666/aeer/1904_32933302
- Corral, J. M., Padilla, J. A., & Izquierdo, M. (2010). Associations between milk protein genetic polymorphisms and milk production traits in Merino sheep breed. Livestock Science, 129(1-3), 73-79. https://doi.org/10.1016/j.livsci.2010.01.007
- Dario, C., Carnicella, D., Dario, M., & Bufano, G. (2008). Genetic polymorphism of β-lactoglobulin gene and effect on milk composition in Leccese sheep. Small Ruminant Research, 74(1-3), 270-273. https://doi.org/10.1016/j.smallrumres.2007.06.007
- Đokić, M., Marković, B., Marković, M., Mirecki, S., Veljić, M., & Perić, L. (2019). Relationship between β-Lactoglobulin gene polymorphism and milk traits and milk composition of Sora sheep breed. In International Symposium on Animal Science (ISAS): Herceg Novi, Montenegro,100-105. https://doi:10.17707/AgricultForest.66.3.02
- Eignatev, G. (1998). Genetic polymorphisms of milk proteins in Russian sheep breeds and crosses. [Doctoral Dissertation, Moscow University, Russia].
- Erhardt, G. (1989). Evidence for a third allele at the β-lactoglobulin (BLG) locus of sheep milk and its occurrence in different breeds. Animal Genetics, 20, 197-204. https://doi.org/10.1111/j.1365-2052.1989.tb00857.x
- Fadhil, I. A., & Dakheel, M. H. (2022). Impact of BLG gene polymorphism on some ovine production traits reared in middle part of Iraq. Caspian Journal of Environmental Sciences, 20(5), 1003-1009. https://doi.org/10.22124/CJES.2022.6056
- Falconer, DS., & Mackay, T.E.C. (1996). Introduction to Quantitative Genetics, (4th. ed.) Longman, Essex.
- Feligini, M., Parma, P., Aleandri, R., Greppi, G. F., & Enne, G. (1998). PCR-RFLP test for direct determination of beta-lactoglobulin genotype in sheep. Animal Genetics, 29(6), 473-474. https://doi.org/10.1046/j.1365-2052.1998.296352.x
- Giambra, I.J., Jäger, S., & Erhardt, G. (2010). Isoelectric focusing reveals additional casein variants in German sheep breeds. Small Ruminant Research, 90(1-3), 11-17. https://doi.org/10.1016/j.smallrumres.2009.12.025
- Kawecka, A., & Radko, A. (2011). Genetic polymorphism of β-lactoglobulin in sheep raised for milk production. Journal of Applied Animal Research, 39(1), 68-71. https://doi.org/10.1080/09712119.2011.565223
- Kleyn, D.H., Lynch, J. M., Barbano, D. M., Bloom, M. J., Mitchell, M. W., Cooper, L. S., Cusak, E., Fick, M., Hanks, T., Hesen, M. K., Johnson, J., Kleyn, D. H., Mercer, F., Monahan, D., Peat, B., & Petit, M. (2001). Determination of fat in raw and processed milks by the Gerber method: Collaborative study. Journal of AOAC International, 84(5), 1499-1508. https://doi.org/10.1093/jaoac/84.5.1499
- Koca, D., Turgut, A. O., Çetin, N., Üner, S., Gülendağ, E., & Karagülle, B. (2023). Chemical composition and physical properties of milk in Norduz sheep. Van Veterinary Journal, 34(3), 271-274. https://doi.org/10.36483/vanvetj.1353378
- Kolde, H.J., & Braunitzer, G. (1983). The primary structure of ovine βlactoglobulin. Milckwissenschaft, 38, 70-72.
- Komprej, A., Gorjanc, G., Kompan, D., & Kovač, M. (2012). Lactation curves for milk yield, fat, and protein content in Slovenian dairy sheep. Czech Journal of Animal Science, 57(5), 231-239. https://doi:10.17221/5921-CJAS
- Kusza, S., Ilie, D. E., Sauer, M., Nagy, K., Atanasiu, T. S., & Gavojdian, D. (2018). Study of LGB gene polymorphisms of small ruminants reared in Eastern Europe. Czech Journal of Animal Science, 63(4), 152-159. https://doi:10.17221/93/2017-CJAS
- Mele, M., Conte, G., Serra, A., Buccioni, A., & Secchiari, P. (2007). Relationship between beta-lactoglobulin polymorphism and milk fatty acid composition in milk of Massese dairy ewes. Small Ruminant Research, 73(1-3), 37-44. https://doi.org/10.1016/j.smallrumres.2006.10.021
- Michalcova, A., & Krupova, Z. (2009). Influence of β-lactoglobulin C genotypes on composition of milk and milk production traits of the Slovak ovine breeds. Acta fytotechnica et zootechnica–Mimoriadne číslo Nitra, Slovaca Universitas Agriculturae Nitriae, 12, 438-446.
- Mohammadi, A., Nassiry, M.R., Elyasi, G., & Shodja, J. (2006). Genetic polymorphism of β-lactoglobulin in certain Iranian and Russian sheep breeds. Iranian Journal of Biotechnology, 4, 265-268
- Othman, O. E., El Fiky, S. A., Hassan, N. A., Mahfouz, E. R., & Balabel, E. A. (2012). Genetic polymorphism of whey protein genes 𝛽-LG and 𝛼-LA in three Egyptian sheep breeds. Journal of Applied Biological Sciences, 6(3), 25-30.
- Özmen, Ö., & Kul, S. (2016). Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 323-328.
- Russel, A.J.F., Doney, J.M., & Gunn, R.G. (1969). Subjective assessment of body fat in live sheep. Journal of Agricultural Science (Cambridge) 72, 451–454. https://doi.org/10.1017/S0021859600024874
- Rustempašić, A., Dokso, A., Zečević, E., Hodžić, A., Hrković-Porobija, A., Sarić, Z., Miskoska–Milevska, E., Popovski, Z., & Brka, M. (2018). Polymorphism of β-lactoglobulin in Pramenka sheep breed in Bosnia and Herzegovina. The Journal of Animal & Plant Sciences, 28(1), 337-340.
- Selvaggi, M., Laudadio, V., Dario, C., & Tufarelli, V. (2014). Investigating the genetic polymorphism of sheep milk proteins: A useful tool for dairy production. Journal of the Science of Food and Agriculture, 94(15), 3090-3099. https://doi.org/10.1002/jsfa.6750
- Staiger, E. A., Thonney, M. L., Buchanan, J. W., Rogers, E. R., Oltenacu, P. A., & Mateescu, R. G. (2010). Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep. Journal of Dairy Science, 93(4), 1736-1742. https://doi.org/10.3168/jds.2009-2630
- Stambekov, S., Shapiro, Y. O., & Mandrusova, E.E. (1997). Milk protein polymorphism in Soviet Merino and Latvian Dark headed ewes and its connection with economically valuable characteristics. Journal of Dairy Science, 39, 6873.
- Sumantri, C., Nurhayati, D., Farajallah, A., & Anggraeni, A. (2008). Association between polymorphism of β-lactoglobulin gene on milk yield and quality in local sheep at jonggol animal science teaching and research unit (Jastru). In Annales Bogorienses, 12(1), 17-24.
- Triantaphyllopoulos, K. A., Koutsouli, P., Kandris, A., Papachristou, D., Markopoulou, K. E., Mataragka, A., Massouras, M., & Bizelis, I. (2017). Effect of β-lactoglobulin gene polymorphism, lactation stage and breed on milk traits in Chios and Karagouniko sheep breeds. Annals of Animal Science, 17(2), 371-384. https://doi.org/10.1515/aoas-2016-0058
- Wendorff, W. L., & Haenlein, G. F. W. (2017). Sheep milk-composition and nutrition. In. Young, W. P., George, F. W. H., & William, L. (Eds), Handbook of Milk of Non-Bovine Mammals (pp. 210-221). JohnWiley & Sons Ltd.
- Yousefi, S., Azari, M. A., Zerehdaran, S., Samiee, R., & Khataminejhad, R. (2013). Effect of β-lactoglobulin and κ-casein genes polymorphism on milk composition in indigenous Zel sheep. Archives Animal Breeding, 56(1), 216-224. https://doi.org/10.7482/0003-9438-56-021
- TUIK., (2022, 5 May). Çiğ Süt Üretim İstatistikleri 2022. https://data.tuik.gov.tr/Bulten/Index?p=%C3%87i%C4%9F-S%C3%BCt-%C3%9Cretim%C4%B0statistik leri-2022-49699&dil=1
- Turgut, A. O., Gulendağ, E, Koca, D., & Üner, S. (2023). Milk composition traits of Hamdani cross-bred sheep raised under extensive management. ISPEC Journal of Agricultural Science, 7(2), 271-279. https://doi.org/10.5281/zenodo.8020354
Year 2024,
Volume: 9 Issue: 1, 35 - 41, 30.04.2024
Abstract
Project Number
2022-SIUVET-04
References
- Anton, Z., Istvan, K., Attila, M., Sandor, F., Andras, L. (1998). Genetic polymorphisms of milk proteins in Hungarian dairy sheep breeds and crosses, Sheep and Goat Production in Central and Eastern European Countries, Hungary. REU Technical Series, 50, 224-226.
- Baranyi, M., Kerekes, A., Hiripi, L., & Bősze, Z. (2010). Preliminary data on beta-lactoglobulin genetic polymorphisms in Hungarian Awassi and Racka sheep. Scientific Papers Animal Science and Biotechnologies, 43, 1-4.
- Barbano, D. M., Clark, J. L., Dunham, C. E., & Flemin, R. J. (1990). Kjeldahl method for determination of total nitrogen content of milk: collaborative study. Journal of the Association of Official Analytical Chemists, 73(6), 849-859. https://doi.org/10.1093/jaoac/73.6.849
- Bayraktar, M., & Shoshin, O. (2021). Determination effects of slc27a3 and β-lactoglobulin gene polymorphisms on the milk composition in Hamdani sheep. Applied Ecology and Environmental Research, 19(4), 3293-3302. http://dx.doi.org/10. 15666/aeer/1904_32933302
- Corral, J. M., Padilla, J. A., & Izquierdo, M. (2010). Associations between milk protein genetic polymorphisms and milk production traits in Merino sheep breed. Livestock Science, 129(1-3), 73-79. https://doi.org/10.1016/j.livsci.2010.01.007
- Dario, C., Carnicella, D., Dario, M., & Bufano, G. (2008). Genetic polymorphism of β-lactoglobulin gene and effect on milk composition in Leccese sheep. Small Ruminant Research, 74(1-3), 270-273. https://doi.org/10.1016/j.smallrumres.2007.06.007
- Đokić, M., Marković, B., Marković, M., Mirecki, S., Veljić, M., & Perić, L. (2019). Relationship between β-Lactoglobulin gene polymorphism and milk traits and milk composition of Sora sheep breed. In International Symposium on Animal Science (ISAS): Herceg Novi, Montenegro,100-105. https://doi:10.17707/AgricultForest.66.3.02
- Eignatev, G. (1998). Genetic polymorphisms of milk proteins in Russian sheep breeds and crosses. [Doctoral Dissertation, Moscow University, Russia].
- Erhardt, G. (1989). Evidence for a third allele at the β-lactoglobulin (BLG) locus of sheep milk and its occurrence in different breeds. Animal Genetics, 20, 197-204. https://doi.org/10.1111/j.1365-2052.1989.tb00857.x
- Fadhil, I. A., & Dakheel, M. H. (2022). Impact of BLG gene polymorphism on some ovine production traits reared in middle part of Iraq. Caspian Journal of Environmental Sciences, 20(5), 1003-1009. https://doi.org/10.22124/CJES.2022.6056
- Falconer, DS., & Mackay, T.E.C. (1996). Introduction to Quantitative Genetics, (4th. ed.) Longman, Essex.
- Feligini, M., Parma, P., Aleandri, R., Greppi, G. F., & Enne, G. (1998). PCR-RFLP test for direct determination of beta-lactoglobulin genotype in sheep. Animal Genetics, 29(6), 473-474. https://doi.org/10.1046/j.1365-2052.1998.296352.x
- Giambra, I.J., Jäger, S., & Erhardt, G. (2010). Isoelectric focusing reveals additional casein variants in German sheep breeds. Small Ruminant Research, 90(1-3), 11-17. https://doi.org/10.1016/j.smallrumres.2009.12.025
- Kawecka, A., & Radko, A. (2011). Genetic polymorphism of β-lactoglobulin in sheep raised for milk production. Journal of Applied Animal Research, 39(1), 68-71. https://doi.org/10.1080/09712119.2011.565223
- Kleyn, D.H., Lynch, J. M., Barbano, D. M., Bloom, M. J., Mitchell, M. W., Cooper, L. S., Cusak, E., Fick, M., Hanks, T., Hesen, M. K., Johnson, J., Kleyn, D. H., Mercer, F., Monahan, D., Peat, B., & Petit, M. (2001). Determination of fat in raw and processed milks by the Gerber method: Collaborative study. Journal of AOAC International, 84(5), 1499-1508. https://doi.org/10.1093/jaoac/84.5.1499
- Koca, D., Turgut, A. O., Çetin, N., Üner, S., Gülendağ, E., & Karagülle, B. (2023). Chemical composition and physical properties of milk in Norduz sheep. Van Veterinary Journal, 34(3), 271-274. https://doi.org/10.36483/vanvetj.1353378
- Kolde, H.J., & Braunitzer, G. (1983). The primary structure of ovine βlactoglobulin. Milckwissenschaft, 38, 70-72.
- Komprej, A., Gorjanc, G., Kompan, D., & Kovač, M. (2012). Lactation curves for milk yield, fat, and protein content in Slovenian dairy sheep. Czech Journal of Animal Science, 57(5), 231-239. https://doi:10.17221/5921-CJAS
- Kusza, S., Ilie, D. E., Sauer, M., Nagy, K., Atanasiu, T. S., & Gavojdian, D. (2018). Study of LGB gene polymorphisms of small ruminants reared in Eastern Europe. Czech Journal of Animal Science, 63(4), 152-159. https://doi:10.17221/93/2017-CJAS
- Mele, M., Conte, G., Serra, A., Buccioni, A., & Secchiari, P. (2007). Relationship between beta-lactoglobulin polymorphism and milk fatty acid composition in milk of Massese dairy ewes. Small Ruminant Research, 73(1-3), 37-44. https://doi.org/10.1016/j.smallrumres.2006.10.021
- Michalcova, A., & Krupova, Z. (2009). Influence of β-lactoglobulin C genotypes on composition of milk and milk production traits of the Slovak ovine breeds. Acta fytotechnica et zootechnica–Mimoriadne číslo Nitra, Slovaca Universitas Agriculturae Nitriae, 12, 438-446.
- Mohammadi, A., Nassiry, M.R., Elyasi, G., & Shodja, J. (2006). Genetic polymorphism of β-lactoglobulin in certain Iranian and Russian sheep breeds. Iranian Journal of Biotechnology, 4, 265-268
- Othman, O. E., El Fiky, S. A., Hassan, N. A., Mahfouz, E. R., & Balabel, E. A. (2012). Genetic polymorphism of whey protein genes 𝛽-LG and 𝛼-LA in three Egyptian sheep breeds. Journal of Applied Biological Sciences, 6(3), 25-30.
- Özmen, Ö., & Kul, S. (2016). Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 323-328.
- Russel, A.J.F., Doney, J.M., & Gunn, R.G. (1969). Subjective assessment of body fat in live sheep. Journal of Agricultural Science (Cambridge) 72, 451–454. https://doi.org/10.1017/S0021859600024874
- Rustempašić, A., Dokso, A., Zečević, E., Hodžić, A., Hrković-Porobija, A., Sarić, Z., Miskoska–Milevska, E., Popovski, Z., & Brka, M. (2018). Polymorphism of β-lactoglobulin in Pramenka sheep breed in Bosnia and Herzegovina. The Journal of Animal & Plant Sciences, 28(1), 337-340.
- Selvaggi, M., Laudadio, V., Dario, C., & Tufarelli, V. (2014). Investigating the genetic polymorphism of sheep milk proteins: A useful tool for dairy production. Journal of the Science of Food and Agriculture, 94(15), 3090-3099. https://doi.org/10.1002/jsfa.6750
- Staiger, E. A., Thonney, M. L., Buchanan, J. W., Rogers, E. R., Oltenacu, P. A., & Mateescu, R. G. (2010). Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep. Journal of Dairy Science, 93(4), 1736-1742. https://doi.org/10.3168/jds.2009-2630
- Stambekov, S., Shapiro, Y. O., & Mandrusova, E.E. (1997). Milk protein polymorphism in Soviet Merino and Latvian Dark headed ewes and its connection with economically valuable characteristics. Journal of Dairy Science, 39, 6873.
- Sumantri, C., Nurhayati, D., Farajallah, A., & Anggraeni, A. (2008). Association between polymorphism of β-lactoglobulin gene on milk yield and quality in local sheep at jonggol animal science teaching and research unit (Jastru). In Annales Bogorienses, 12(1), 17-24.
- Triantaphyllopoulos, K. A., Koutsouli, P., Kandris, A., Papachristou, D., Markopoulou, K. E., Mataragka, A., Massouras, M., & Bizelis, I. (2017). Effect of β-lactoglobulin gene polymorphism, lactation stage and breed on milk traits in Chios and Karagouniko sheep breeds. Annals of Animal Science, 17(2), 371-384. https://doi.org/10.1515/aoas-2016-0058
- Wendorff, W. L., & Haenlein, G. F. W. (2017). Sheep milk-composition and nutrition. In. Young, W. P., George, F. W. H., & William, L. (Eds), Handbook of Milk of Non-Bovine Mammals (pp. 210-221). JohnWiley & Sons Ltd.
- Yousefi, S., Azari, M. A., Zerehdaran, S., Samiee, R., & Khataminejhad, R. (2013). Effect of β-lactoglobulin and κ-casein genes polymorphism on milk composition in indigenous Zel sheep. Archives Animal Breeding, 56(1), 216-224. https://doi.org/10.7482/0003-9438-56-021
- TUIK., (2022, 5 May). Çiğ Süt Üretim İstatistikleri 2022. https://data.tuik.gov.tr/Bulten/Index?p=%C3%87i%C4%9F-S%C3%BCt-%C3%9Cretim%C4%B0statistik leri-2022-49699&dil=1
- Turgut, A. O., Gulendağ, E, Koca, D., & Üner, S. (2023). Milk composition traits of Hamdani cross-bred sheep raised under extensive management. ISPEC Journal of Agricultural Science, 7(2), 271-279. https://doi.org/10.5281/zenodo.8020354
There are 35 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Obstetrics and Gynecology, Veterinary Food Hygiene and Technology, Animal Science, Genetics and Biostatistics |
| Journal Section | Research Articles |
| Authors | |
| Project Number | 2022-SIUVET-04 |
| Early Pub Date | April 21, 2024 |
| Publication Date | April 30, 2024 |
| Submission Date | December 9, 2023 |
| Acceptance Date | February 14, 2024 |
| Published in Issue | Year 2024 Volume: 9 Issue: 1 |
