Evaluation of the variations in spermatological parameters of Arabian stallions according to different periods of breeding season
Abstract
In this study, determining of the variations on spermatological characteristics of Arabian stallion semen in the different periods of the breeding season is aimed. The periods have been described as the beginning of the season (BS: 15th February – 15th March), mid-season (MS: 15th April – 15th May), and end of the season (ES: 30th May – 30th June). A total of 7 Arabian stallions that were 5-10 years old, with body condition scores between 3-3.5 and without any problems in terms of general and reproductive health, were enrolled in the study. In total, three semen samples were collected from each stallion, once in each period. Then, these samples were evaluated in terms of volume, concentration, total motility (TMOT), progressive motility (PMOT), viability, high mitochondrial membrane potential (HMMP), acrosome integrity (AI), capacitation index (Ci), and lipid peroxidation (LPO) parameters, which were analyzed by computer assisted sperm analysis and flowcytometry devices. As a result, the differences in volume, concentration, TMOT, and PMOT between the BS, MS, and ES periods were statistically insignificant (p>0.05). The lowest viability values were determined in the BS period (p<0.001). The highest values of the HMMP were obtained in the MS (p<0.05). The AI results were higher in the ES period than the others (p<0.05). While the Ci (p<0.01) and LP (p<0.001) parameters had the highest values in the BS period, a gradual decrease has been observed until the ES period. In conclusion, it has been observed that the best semen quality can be obtained in the period of MS (15 April – 15 May) for young (5-10) aged Arabian stallions breeding in Türkiye.
Keywords
Arabian stallion breeding season computer assisted sperm analysis flowcytometry semen quality
Ethical Statement
Ankara University Animal Experiments Local Ethics Committee (No:2022-20-178)
Supporting Institution
The Scientific and Technological Research Council of Turkiye (TUBITAK)
Project Number
122O897
References
- Gamboa S, Rodrigues AS, Henriques L, Batista C, Ramalho-Santos J. Seasonal functional relevance of sperm characteristics in equine spermatozoa. Theriogenology 2010;73:950-958.
- Chemineau P, Guillaume D, Migaud M, Thiéry JC, Pellicer‐Rubio MT, Malpaux B. Seasonality of reproduction in mammals: intimate regulatory mechanisms and practical implications. Reproduction in Domestic Animals 2008;43:40-47.
- Johnson L. Seasonal differences in equine spermatocytogenesis Biology of Reproduction 1991;44:284-291.
- Hoffmann B, Landeck A. Testicular endocrine function, seasonality and semen quality of the stallion. Animal Reproduction Science 1999;57:89-98.
- Clay CM, Squires EL, Amann RP, Nett TM. Influences of season and artificial photoperiod on stallions: luteinizing hormone follicle-stimulating hormone and testosterone. Journal of Animal Science 1988;66:1246-1255.
- Morte MI, Rodrigues AM, Soares D, Rodrigues AS, Gamboa S, Ramalho-Santos J. The quantification of lipid and protein oxidation in stallion spermatozoa and seminal plasma: seasonal distinctions and correlations with DNA strand breaks, classical seminal parameters and stallion fertility. Animal Reproduction Science 2008;106:36-47.
- Mislei B, Bucci D, Malama E, Bollwein H, Mari G. Seasonal changes in ROS concentrations and sperm quality in unfrozen and frozen-thawed stallion semen. Theriogenology 2020;144:89-97.
- Janett F, Thun R, Niederer K, Burger D, Hässig M. Seasonal changes in semen quality and freezability in the Warmblood stallion. Theriogenology 2003;60:453-461.
- Aurich C. Reprint of: Seasonal influences on cooled-shipped and frozen-thawed stallion semen. Journal of Equine Veterinary Science 2016;43:1-5.
- Pruß D, Oldenhof H, Wolkers WF, Sieme H. Alginate encapsulation of stallion sperm for increasing storage stability. Animal Reproduction Science 2022;238:106945.
- Jasko DJ. Evaluation of stallion semen. Veterinary Clinics of North America: Equine Practice 1992;8:129-148.
- Crespo F, Wilson R, Díaz-Jimenez M, Consuegra C, Dorado J, Barrado BG, et al. Effect of season on individual stallion semen characteristics. Animal Reproduction Science 2020;223:106641.
- Wrench N, Pinto CR, Klinefelter GR, Dix DJ, Flowers WL, Farin CE. Effect of season on fresh and cryopreserved stallion semen. Animal Reproduction Science 2010;119:219-227.
Arap aygırlarında spermatolojik parametrelerin sezonun farklı dönemlerine göre değişimlerinin değerlendirilmesi
Abstract
Bu çalışmada, Arap aygırlarında aşım sezonu içerisinde farklı dönemlerde spermatolojik parametrelerde gözlemlenen farklılıkların belirlenmesi amaçlanmıştır. Aşım sezonu içerisindeki farklı dönemler sezon başı (SB: 15 Şubat – 15 Mart), sezon ortası (SO: 15 Nisan – 15 Mayıs) ve sezon sonu (SS: 30 Mayıs – 30 Haziran) olarak belirlenmiştir. Yaşları 5-10 arasında değişen, vücut kondisyon skorları 3-3,5 arasında olan, genel ve reprodüktif sağlık durumları açısından herhangi bir problem olmayan toplam 7 Arap aygırı çalışmaya dahil edildi. Her bir aygırdan, her bir dönemde bir defa olmak üzere, toplam 3 sperma alma işlemi gerçekleştirildi. Alınan spermalar miktar, yoğunluk, toplam motilite (TMOT), progresif motilite (PMOT), canlılık, akrozom sağlamlığı (AI), yüksek mitokondriyal membran potansiyeli (HMMP), kapasitasyon indeksi (Ci) ve lipid peroksidasyon seviyesi (LPO) parametreleri açısından bilgisayar yardımlı sperma analizi ve akış sitometri yöntemleriyle analiz edildi. Analiz sonuçlarına göre miktar, yoğunluk TMOT ve PMOT parametrelerinde periyotlar arasındaki farklılıklar istatistiksel olarak benzer bulundu (p>0,05). En düşük canlılık değeri SB periyodunda elde edildi (P<0,001). En yüksek HMMP değeri SO periyodunda bulundu (p<0,05). Akrozom sağlamlığı değeri SS periyodunda diğer periyotlara göre daha yüksekti (p<0,05). Kapasitasyon indeksi (p<0,01) ve LPO (p<0,001) değerleri SB periyodunda en yüksek değerlere sahipken, SS periyoduna gidildikçe kademeli olarak düşüş gösterdi. Sonuç olarak, Türkiye’de yetiştiriciliği yapılan genç yaşlı (5-10) Arap aygırlarında en iyi sperma kalitesinin SO periyodunda (15 Nisan – 15 Mayıs) alınabileceği sonucuna ulaşılmıştır.
Project Number
122O897
References
- Gamboa S, Rodrigues AS, Henriques L, Batista C, Ramalho-Santos J. Seasonal functional relevance of sperm characteristics in equine spermatozoa. Theriogenology 2010;73:950-958.
- Chemineau P, Guillaume D, Migaud M, Thiéry JC, Pellicer‐Rubio MT, Malpaux B. Seasonality of reproduction in mammals: intimate regulatory mechanisms and practical implications. Reproduction in Domestic Animals 2008;43:40-47.
- Johnson L. Seasonal differences in equine spermatocytogenesis Biology of Reproduction 1991;44:284-291.
- Hoffmann B, Landeck A. Testicular endocrine function, seasonality and semen quality of the stallion. Animal Reproduction Science 1999;57:89-98.
- Clay CM, Squires EL, Amann RP, Nett TM. Influences of season and artificial photoperiod on stallions: luteinizing hormone follicle-stimulating hormone and testosterone. Journal of Animal Science 1988;66:1246-1255.
- Morte MI, Rodrigues AM, Soares D, Rodrigues AS, Gamboa S, Ramalho-Santos J. The quantification of lipid and protein oxidation in stallion spermatozoa and seminal plasma: seasonal distinctions and correlations with DNA strand breaks, classical seminal parameters and stallion fertility. Animal Reproduction Science 2008;106:36-47.
- Mislei B, Bucci D, Malama E, Bollwein H, Mari G. Seasonal changes in ROS concentrations and sperm quality in unfrozen and frozen-thawed stallion semen. Theriogenology 2020;144:89-97.
- Janett F, Thun R, Niederer K, Burger D, Hässig M. Seasonal changes in semen quality and freezability in the Warmblood stallion. Theriogenology 2003;60:453-461.
- Aurich C. Reprint of: Seasonal influences on cooled-shipped and frozen-thawed stallion semen. Journal of Equine Veterinary Science 2016;43:1-5.
- Pruß D, Oldenhof H, Wolkers WF, Sieme H. Alginate encapsulation of stallion sperm for increasing storage stability. Animal Reproduction Science 2022;238:106945.
- Jasko DJ. Evaluation of stallion semen. Veterinary Clinics of North America: Equine Practice 1992;8:129-148.
- Crespo F, Wilson R, Díaz-Jimenez M, Consuegra C, Dorado J, Barrado BG, et al. Effect of season on individual stallion semen characteristics. Animal Reproduction Science 2020;223:106641.
- Wrench N, Pinto CR, Klinefelter GR, Dix DJ, Flowers WL, Farin CE. Effect of season on fresh and cryopreserved stallion semen. Animal Reproduction Science 2010;119:219-227.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Urology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | 122O897 |
| Early Pub Date | January 12, 2024 |
| Publication Date | January 15, 2024 |
| Submission Date | September 1, 2023 |
| Acceptance Date | November 14, 2023 |
| Published in Issue | Year 2024 Volume: 95 Issue: 1 |
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