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Effects of Chronically Exposure to Flor and 7,12-Dimethylbenzanthracene (DMBA) on Spermatogenesis and Testicular Histopathology in Rats

Yıl 2018, Cilt: 13 Sayı: 2, 141 - 148, 25.10.2018
https://doi.org/10.17094/ataunivbd.345511

Öz

This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH)

derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testis

histopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment,

intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosterone

levels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratio

of motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in rats

significantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubulus

wall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg / kg)

group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes in

the tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg / kg) were examined,

atrophy in seminiferous tubules, severe degenerative and necrotic changes in spermatogoniums on the walls of tubules and

thinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in the

intertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF and

DMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated.

Kaynakça

  • 1. Baek SO., Field RA., Goldstone ME., Kirk PW., Lester JN., Perry R., 1991. A review of atmospheric polycyclic aromatic hydrocarbons: sources, fate and behavior. Water Air and Soil Pollution, 60, 279-300.
  • 2. Carrera G., Fernandez P., Vilanova RM., Grimalt JO., 2001. Persistent organic pollutants in snow from European high mountain areas. Atmospheric Environment, 35, 245-254.
  • 3. Choubisa SL., 1999. Some observations on endemic fluorosis in domestic animals in Southern Rajasthan (India). Vet Res Com, 23(7), 457-465.
  • 4. Choi AL., Sun G., Zhang T., Grandjean P., 2012. Developmental fluoride neurotoxicity: a systemic review and meta-analysis. Environ Health Perspect, 120(10), 1362-1368.
  • 5. Cinar DA., Selcuk M., 2005. Effects of chronic fluorosis on thyroxine, triiodothyronine, and protein-bound iodine in cows. Fluoride, 38(1), 65-68.
  • 6. Comba B., Cinar DA., 2016. Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Üniversitesi Vet Fak Derg, 63, 223-227.
  • 7. Dickhut RM., Gustafson KE., 1995. Atmospheric washout of polycyclic aromatic hydrocarbons in the Southern Chesapeake Bay region. Environmental Science and Technology, 29(6), 1518-1525.
  • 8. Dobaradaran S., Fazelinia F., Mahvi AH., Hosseini SS., 2009. Particulate airborne fluoride from an aluminium production plant in Arak, Iran. Fluoride, 42(3), 228-232.
  • 9. Donmez N., Cinar DA., 2003. Effects of chronic fluorosis on electrocardiogram in sheep. Biol Trace Elem Res, 92(2), 115-122.
  • 10. Drózdz M., Kucharz E., Grucka-Mamczar E., 1980. Effect of sodium fluoride on colla-gen content in skin and lungs of growing rats. Acta Biologica Medical Germainca, 39, 287–293.
  • 11. Ersan Y., Koç E., Ari İ., Karademir B., 2010. Histopathological effects of chronic fluorosis on the liver of mice (Swiss albino). Turkish Journal of Medical Sciences, 40(4), 619-622.
  • 12. Hillery BR., Simcik MF., Basu I., Hoff RM., Strachan WMJ., Burniston D., Chan CH., Brice KA., Sweet CW., Hites RA., 1998. Atmospheric deposition of toxic pollutants to the Great Lakes as measured by the integrated atmospheric deposition network. Environmental Science and Technology, 32, 2216-2221.
  • 13. Kilicalp D., Cinar A., Belge F., 2004. Effects of Chronic fluorosis on electrocardiogram in dogs. Fluoride, 37(2), 96-101.
  • 14. Li Y., Dunipace AJ., Stookey GK., 1987. Effect of fluoride on the mouse sperm morphology test. Journal of Denteal Research, 66, 1509-1511.
  • 15. Mert H., Comba B., Mert N., Çınar DA., Apaydın Yıldırım B., 2016. Advanced oxidation protein products AOPP levels and kidney function in fluorotic sheep. Fluoride, 49, 336-342.
  • 16. Narayana MV., Chinoy NJ., 1994. Effect of fluoride on rat testicular steroidogenesis. Fluoride, 27, 7-12.
  • 17. Oncu M., Kocak A., Karaoz E., Darici H., Savik E., Gultekin F., 2007. Effect of long-term fluoride exposure on lipid peroxidation and histology of testes in first andsecond generation rats. Biological Trace Element Research, 118, 260–268.
  • 18. Ozdemir H., Oto G., Ekin S., Yener Z., Yildirim S., 2017. The protective effects of Lepidium sativum l. In 7,12-dimethylbenz(a)anthracene applıed rats. Fresenius Environmental Bulletin, 26(3), 2446-2453.
  • 19. Sonmez M., Turk G., Yuce A., 2005. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology, 63, 2063-2072.
  • 20. Suska M., 2002. Energy metabolism of erythrocytes in lambs chronically exposed to fluorine compounds. Acta Vet Brno, 71, 313-317.
  • 21. Tokar VI., Savchenko ON., 1977. The ifluence of inorganic flourine compounds on functional condition of the hypopphysis-tetis system. Problems of Endocrinology, 23, 104-107.
  • 22. Turk G., Atessahin A., Sonmez M., Ceribasi AO., Yuce A., 2008.Improvement of cisplatin-induced injuries to sperm quality, the oxidant–antioxidant system, and the histologic structure of the rat testis by ellagic acid. Fertil Steril, 89, 1474-1481.
  • 23. Vogel E., 1973. Strong antimutagenic effect of flüoride on mutation induction by trenimon and 1-phenyl-3-dimethyltriazene in Drosophila melanogaster. Mutation Research, 20, 339-352.
  • 24. Wang JL., Zhang YM., Zhang HJ., Zhang K., Zhang ZW., Li J., 2009. Toxic effectsof fluoride on reproductive ability in male rats, sperm motility, oxidative stress,cell cycle, and testicular apoptosis. Fluoride, 42,174–178.
  • 25. Yildirim S., Oto G., Comba B., Ekin S., Cinar DA., 2017a. The investigation of protective effects of resveratrol on biochemical and histopathological parameters in induced experimentally chronic fluorosis in rats. Fluoroide, 50(2 Pt 2): (in press).
  • 26. Yildirim S., Ekin S., Huyut Z., Oto G., Comba A., Uyar H., Sengul E., Cinar DA., 2017b. Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and histopathological findings in rats. Fluoroide, 50(3 Pt 2): (in press).

Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri

Yıl 2018, Cilt: 13 Sayı: 2, 141 - 148, 25.10.2018
https://doi.org/10.17094/ataunivbd.345511

Öz

Bu çalışmada, sıçanlarda florür ve polisiklik aromatik hidrokarbon (PAH) türevi olan 7,12-dimetilbenz [a] antrasen (DMBA)'in spermatogenesis ve testisin histopatolojisi üzerine tek tek ve kombinasyon halinde etkilerinin araştırılması amaçlandı. 250-300 g ağırlığında 40 erkek Wistar albino sıçanı kullanıldı. Deneyin 90. gününde intrakardiak kan örnekleri ve testis doku örnekleri alındı. Gruplarda ki FSH ve LH düzeylerinde her hangi bir değişiklik gözükmezken testosteron düzeylerinin kontrol grubuna göre diğer gruplarda önemli ölçüde düştüğü belirlendi (P<0.001). Kronik flor ve DMBA toksikasyonu ratlarda sperm motilite oranını, yoğunluğunu önemli ölçüde düşürmekte ve anormal sperm oranı ise yükseltmektedir. Histopatolojik incelemelerde; NaF (30 ppm) grubunda ki ratların testislerinin tubulus duvarında spermatosit ve spermatozoa sayısında azalma görüldü. DMBA (10 mg/kg) grubunda bulunan ratların testis dokuları incelendiğinde, tubuluslarda atrofi, intertubuler aralıklarda ödem ve tubulus duvarında bulunan spermatosit sayısında şidettli azalma tespit edildi. NaF (30 ppm) + DMBA (10 mg/kg) grubunda ki ratların testis dokuları incelendiğinde ise, seminifer tubullerde atrofi, tubullerin duvarında spermatogoniumlarda şiddetli dejeneratif ve nekrotik değişiklikler ve buna bağlı olarakta tubul duvarlarında incelme saptandı. Tubul lümenlerinde spermatozoa rastlanmadı. İntertubuler aralıklarda şiddetli ödem, bu bölgelerde bulunan damarlarda dilatasyon ve hiperemi saptandı. Sonuç olarak NaF ve DMBA nın testis dokusu ve döl verimi üzerine her birinin tek tek olumsuz etki ettiği ve birlikte kombinasyon halinde ise bu etkinin daha da şiddetlendiği görüldü.

Kaynakça

  • 1. Baek SO., Field RA., Goldstone ME., Kirk PW., Lester JN., Perry R., 1991. A review of atmospheric polycyclic aromatic hydrocarbons: sources, fate and behavior. Water Air and Soil Pollution, 60, 279-300.
  • 2. Carrera G., Fernandez P., Vilanova RM., Grimalt JO., 2001. Persistent organic pollutants in snow from European high mountain areas. Atmospheric Environment, 35, 245-254.
  • 3. Choubisa SL., 1999. Some observations on endemic fluorosis in domestic animals in Southern Rajasthan (India). Vet Res Com, 23(7), 457-465.
  • 4. Choi AL., Sun G., Zhang T., Grandjean P., 2012. Developmental fluoride neurotoxicity: a systemic review and meta-analysis. Environ Health Perspect, 120(10), 1362-1368.
  • 5. Cinar DA., Selcuk M., 2005. Effects of chronic fluorosis on thyroxine, triiodothyronine, and protein-bound iodine in cows. Fluoride, 38(1), 65-68.
  • 6. Comba B., Cinar DA., 2016. Investigation of effects of fluorosis on some minerals and hormones in sheep. Ankara Üniversitesi Vet Fak Derg, 63, 223-227.
  • 7. Dickhut RM., Gustafson KE., 1995. Atmospheric washout of polycyclic aromatic hydrocarbons in the Southern Chesapeake Bay region. Environmental Science and Technology, 29(6), 1518-1525.
  • 8. Dobaradaran S., Fazelinia F., Mahvi AH., Hosseini SS., 2009. Particulate airborne fluoride from an aluminium production plant in Arak, Iran. Fluoride, 42(3), 228-232.
  • 9. Donmez N., Cinar DA., 2003. Effects of chronic fluorosis on electrocardiogram in sheep. Biol Trace Elem Res, 92(2), 115-122.
  • 10. Drózdz M., Kucharz E., Grucka-Mamczar E., 1980. Effect of sodium fluoride on colla-gen content in skin and lungs of growing rats. Acta Biologica Medical Germainca, 39, 287–293.
  • 11. Ersan Y., Koç E., Ari İ., Karademir B., 2010. Histopathological effects of chronic fluorosis on the liver of mice (Swiss albino). Turkish Journal of Medical Sciences, 40(4), 619-622.
  • 12. Hillery BR., Simcik MF., Basu I., Hoff RM., Strachan WMJ., Burniston D., Chan CH., Brice KA., Sweet CW., Hites RA., 1998. Atmospheric deposition of toxic pollutants to the Great Lakes as measured by the integrated atmospheric deposition network. Environmental Science and Technology, 32, 2216-2221.
  • 13. Kilicalp D., Cinar A., Belge F., 2004. Effects of Chronic fluorosis on electrocardiogram in dogs. Fluoride, 37(2), 96-101.
  • 14. Li Y., Dunipace AJ., Stookey GK., 1987. Effect of fluoride on the mouse sperm morphology test. Journal of Denteal Research, 66, 1509-1511.
  • 15. Mert H., Comba B., Mert N., Çınar DA., Apaydın Yıldırım B., 2016. Advanced oxidation protein products AOPP levels and kidney function in fluorotic sheep. Fluoride, 49, 336-342.
  • 16. Narayana MV., Chinoy NJ., 1994. Effect of fluoride on rat testicular steroidogenesis. Fluoride, 27, 7-12.
  • 17. Oncu M., Kocak A., Karaoz E., Darici H., Savik E., Gultekin F., 2007. Effect of long-term fluoride exposure on lipid peroxidation and histology of testes in first andsecond generation rats. Biological Trace Element Research, 118, 260–268.
  • 18. Ozdemir H., Oto G., Ekin S., Yener Z., Yildirim S., 2017. The protective effects of Lepidium sativum l. In 7,12-dimethylbenz(a)anthracene applıed rats. Fresenius Environmental Bulletin, 26(3), 2446-2453.
  • 19. Sonmez M., Turk G., Yuce A., 2005. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology, 63, 2063-2072.
  • 20. Suska M., 2002. Energy metabolism of erythrocytes in lambs chronically exposed to fluorine compounds. Acta Vet Brno, 71, 313-317.
  • 21. Tokar VI., Savchenko ON., 1977. The ifluence of inorganic flourine compounds on functional condition of the hypopphysis-tetis system. Problems of Endocrinology, 23, 104-107.
  • 22. Turk G., Atessahin A., Sonmez M., Ceribasi AO., Yuce A., 2008.Improvement of cisplatin-induced injuries to sperm quality, the oxidant–antioxidant system, and the histologic structure of the rat testis by ellagic acid. Fertil Steril, 89, 1474-1481.
  • 23. Vogel E., 1973. Strong antimutagenic effect of flüoride on mutation induction by trenimon and 1-phenyl-3-dimethyltriazene in Drosophila melanogaster. Mutation Research, 20, 339-352.
  • 24. Wang JL., Zhang YM., Zhang HJ., Zhang K., Zhang ZW., Li J., 2009. Toxic effectsof fluoride on reproductive ability in male rats, sperm motility, oxidative stress,cell cycle, and testicular apoptosis. Fluoride, 42,174–178.
  • 25. Yildirim S., Oto G., Comba B., Ekin S., Cinar DA., 2017a. The investigation of protective effects of resveratrol on biochemical and histopathological parameters in induced experimentally chronic fluorosis in rats. Fluoroide, 50(2 Pt 2): (in press).
  • 26. Yildirim S., Ekin S., Huyut Z., Oto G., Comba A., Uyar H., Sengul E., Cinar DA., 2017b. Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and histopathological findings in rats. Fluoroide, 50(3 Pt 2): (in press).
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Serkan Yıldırım

Saadet Belhan

Hasan Uyar Bu kişi benim

Zübeyr Huyut

Gökhan Oto

Yavuz Selim Sağlam

Yayımlanma Tarihi 25 Ekim 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 13 Sayı: 2

Kaynak Göster

APA Yıldırım, S., Belhan, S., Uyar, H., Huyut, Z., vd. (2018). Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13(2), 141-148. https://doi.org/10.17094/ataunivbd.345511
AMA Yıldırım S, Belhan S, Uyar H, Huyut Z, Oto G, Sağlam YS. Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. Ekim 2018;13(2):141-148. doi:10.17094/ataunivbd.345511
Chicago Yıldırım, Serkan, Saadet Belhan, Hasan Uyar, Zübeyr Huyut, Gökhan Oto, ve Yavuz Selim Sağlam. “Kronik Olarak Flor Ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis Ve Testisin Histopatolojisine Etkileri”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13, sy. 2 (Ekim 2018): 141-48. https://doi.org/10.17094/ataunivbd.345511.
EndNote Yıldırım S, Belhan S, Uyar H, Huyut Z, Oto G, Sağlam YS (01 Ekim 2018) Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13 2 141–148.
IEEE S. Yıldırım, S. Belhan, H. Uyar, Z. Huyut, G. Oto, ve Y. S. Sağlam, “Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 13, sy. 2, ss. 141–148, 2018, doi: 10.17094/ataunivbd.345511.
ISNAD Yıldırım, Serkan vd. “Kronik Olarak Flor Ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis Ve Testisin Histopatolojisine Etkileri”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13/2 (Ekim 2018), 141-148. https://doi.org/10.17094/ataunivbd.345511.
JAMA Yıldırım S, Belhan S, Uyar H, Huyut Z, Oto G, Sağlam YS. Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018;13:141–148.
MLA Yıldırım, Serkan vd. “Kronik Olarak Flor Ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis Ve Testisin Histopatolojisine Etkileri”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 13, sy. 2, 2018, ss. 141-8, doi:10.17094/ataunivbd.345511.
Vancouver Yıldırım S, Belhan S, Uyar H, Huyut Z, Oto G, Sağlam YS. Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018;13(2):141-8.