Araştırma Makalesi
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Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan'ın Postoperatif Peritoneal Adezyon Üzerine Etkisi

Yıl 2021, Cilt: 16 Sayı: 1, 41 - 48, 26.04.2021

Öz

Sunulan çalışmada ovaryohisterektomi operasyonundan hemen sonra intraperitonal yolla kitosan kullanımının postoperatif dönemde peritonal adezyon oluşumunun engellemesi üzerine olan etkisini araştırmak amaçlanmıştır. Araştırmada 250-350 gr ağırlığında toplam 15 adet dişi Wistar-albino rat kullanıldı. Ratlar operasyon öncesinde ve sonrasında oda ısısında (22±2°C), neminde, 12 saat ışık-12 saat karanlık ortamda barındırıldı. Tekli kafeslerde barındırılan ratlar için laboratuvar hayvanlarındaki standart bakım ve besleme yapıldı. Bu ratlar uygun anestezik yöntemler ile anesteziye alındıktan sonra, ovaryumlar ve uterus standart prosedurlere uygun olarak alındı. Ratlar daha sonra rastgele 2 gruba ayrıldı. Kitosan grubundaki ratlara (n=8) ovaryohisterektomi operasyonundan hemen sonra 50 mg/kg dozda kitosan 2.5 mL serum fizyolojik ile karıştırılarak bir defaya mahsus olmak üzere intraperitonal yolla verildi. Kontrol grubuna (n=7) ise ilk gruptan farklı olarak yalnızca 2.5 mL serum fizyolojik intraperitonal olarak verildi. Operasyon günü, operasyonu takip eden 7 ve 14. günlerde vücut sıcaklıkları kaydedildi. Hayvanlar operasyondan 21 gün sonra yüksek dozda anestezik verilerek ötenazi edildi. Vücut sıcaklıkları bakımından gruplar arasında fark saptanmadı. Adezyon belirlenmeyen rat oranının kitosan grubunda daha yüksek olduğu saptandı. Ayrıca kitosan grubunda adezyon direncinin daha düşük olduğu saptandı. Özellikle adezyon skoru 3 oranının kontrol grubunda daha yüksek olduğu saptandı. Bu sonuçlar intraperitoneal kitosan uygulamasının adezyon görülme oranını ve adezyon direncini düşürdüğünü göstermektedir.

Kaynakça

  • 1. Stone EA., Conrellic G., Sharp NJH., 1993. Ovariohysterectomy: In Slatter, Textbook of Small Animal Surgery. Vol II 2 Edit. W.B. 1303- 1308. Saunders Company.
  • 2. Akırınmade JF., Eyarefe OD., 2012. Enterologic and gynaecologic complications of ovariohysterectomy in the bitch. NVJ, 33, 571- 576.
  • 3. Apparicio M., Vicente WRR., Farias A., Covizzi GJ., Ribeiro APC., Gadelha CRF., 2007. Pyonephrosis following ovariohysterectomy in a bitch: case report. ARS Veterinaria, 23, 19-22.
  • 4. Konovalova MV., Markov PA., Popova GY., Nikitina IR., Shumikhin KV., Kurek DV., Varlamov VP., Popov SV., 2017. Prevention of postoperative adhesions by biodegradable cryogels from pectin and chitosan polysaccharides. J Bioact Compat Pol, 32, 1-16.
  • 5. Zheng Z., Zhang W., Sun W., Li X., Duan J., Cui J., Feng Z., Mansour HM., 2013. Influence of the carboxymethyl chitosan anti-adhesion solution on the TGF-b1 in a postoperative peritoneal adhesion rat. J Mater Sci: Mater Med, 24, 2549- 2559.
  • 6. Aguilar A., Zein N., Harmouch E., Hafdi B., Bornert F., Offner Damien., Clauss F., Fioretti F., Huck O., Benkirane-Jessel B., Hua G., 2019. Application of chitosan in bone and dental engineering. Molecules, 24, 1-17.
  • 7. Rodriguez DF., Mendes FF., Melo RM., Menezes LB., Guimaraes LLB., Silva AS., Rammazzina Filho WA., Moraes AM., Paulo NM., 2019. Polypropylene meshes coated with chitosan/polyethylene glycol for the reconstruction of the abdominal wall: an experimental study in rats. Arq Bras Med Vet Zootec, 71, 1198-1206.
  • 8. Yeo Y., Burdick JA., Highley CB., Marini R., Langer R., Kohane DS., 2006. Peritoneal application of chitosan and UV-cross-linkable chitosan. J Biomed Mater Res A, 78, 668-675.
  • 9. Furuhama K., Onodera T., 1983. A simple technique for repeated blood collection from the tail vein of the rat. J Toxicol Sci, 8, 161-163.
  • 10. Hooker GD., Taylor BM., Driman DK., 1999. Prevention of adhesion formation with use of sodium hyaluronate–based bioresorbable membrane in a rat model of ventral hernia repair with polypropylene mesh-A randomized, controlled study. Surgery, 125, 211-216.
  • 11. Lauder CIW., Garcea G., Strickland A., Maddern GJ., 2011. Use of a modified chitosane-dextran gel to prevent peritoneal adhesions in a rat model. J Surg Res, 171, 877-882.
  • 12. Garrard CL., Clements RH., Nanney L., Davidson JM., Richards WO., 1999. Adhesion formation is reduced after laparoscopic surgery. Surg Endosc, 13, 10-13.
  • 13. Kennedy R., Costain DJ., McAlister VC., Lee TDG., 1996. Prevention of experimental postoperative peritoneal adhesions by N,O-carboxymethyl chitosan. Surgery, 120, 866-870.
  • 14. Çetin M., Ak D., Duran B., Çetin A., Güvenal T., Yanar O., 2003. Use of methylene blue and N,Ocarboxymethyl chitosan to prevent postoperative adhesions in a rat uterine horn model. Fertility and Sterility, 80, 698-701.
  • 15. Costain DJ., Kennedy R., Ciona C., McAlister VC., Lee TD., 1997. Prevention of postsurgical adhesions with N,O-carboxymethyl chitosan: examination of the most efficacious preparation and the effect of N,O-carboxymethyl chitosan on postsurgical healing. Surgery, 121, 314-331.
  • 16. Chung YJ., An SY., Yeon JY., Shim WS., Mo JH., 2016. Effect of a chitosan gel on hemostasis and prevention of adhesion after endoscopic sinus surgery. Clin Exp Otorhinolaryngol, 9, 143-149.
  • 17. Krause TJ., Zazanis GA., McKinnon RD., 1996. Prevention of postoperative adhesions with the chitin derivative N-0-carboxymethylchitosan. Wound Rep Reg, 4, 53-57.
  • 18. Patrulea V., Ostafe V., Borchard G., Jordan O., 2015. Chitosan as a starting material for wound healing applications. Eur J Pharm Biopharm, 97, 417-426.
  • 19. Lin LX., Yuan F., Zhang HH., Liao NN., Luo JW, Sun YL., 2017. Evaluation of surgical anti-adhesion products to reduce postsurgical intra-abdominal adhesion formation in a rat model. PLOS ONE, 12, 1-9.
  • 20. Tian L., Li H., Li Y., Liu K., Sun Y., Cong Z., Luan X., Li Y., Chen J., Wang L., Ren Z., Cong D., Wang H., Pei J., 2018. A combination of chitosan, cellulose, and seaweed polysaccharide inhibits postoperative intra-abdominal adhesion in rats. J Pharmacol Exp Ther, 364, 399-408.

Influence of Chitosan on the Postoperative Peritoneal Adhesion in Rats Undergoing Ovariohysterectomy

Yıl 2021, Cilt: 16 Sayı: 1, 41 - 48, 26.04.2021

Öz

In the present study, it is aimed to investigate the effect of intraperitoneal use of chitosan on the prevention of peritoneal adhesion formation in the postoperative period immediately after ovariohysterectomy. A total of 15 female Wistar-albino rats weighing 250-350 gr were used for this researcher. Rats were housed in room temperature (22±2°C) and humidity, 12h light-dark cycles. The rats housed in single cages were fed with standard laboratory food and water. After the rats were anesthetized with appropriate anesthetic methods, ovarium and uterus were removed according to the procedure standard procedures used in routines. Rats were randomly divided into 2 groups. Chitosan groups (n=8) were injected intraperitoneally as a single dose mixing 2.5 mL of physiological saline solution with a dose of 50 mg/kg of chitosan immediately after OVH operation. Rats in the other group (n=7), which were evaluated as the control group, were given only 2.5 mL of saline intraperitoneally different from the first group. Body temperatures were recorded on the day of the operation (day 0) and on the 7th and 14th day following the operation. The animals were euthanized 21 days after the operation with a high dose of anesthetic. There was no difference between the groups in terms of body temperatures. Rat without adhesion was found to be higher in the chitosan group. In addition, adhesion resistance was found to be lower in chitosan group. Especially, the number of adhesions score 3 was higher in the control group. These results show that intraperitoneal chitosan application decreases the adhesion rate and adhesion resistance.

Kaynakça

  • 1. Stone EA., Conrellic G., Sharp NJH., 1993. Ovariohysterectomy: In Slatter, Textbook of Small Animal Surgery. Vol II 2 Edit. W.B. 1303- 1308. Saunders Company.
  • 2. Akırınmade JF., Eyarefe OD., 2012. Enterologic and gynaecologic complications of ovariohysterectomy in the bitch. NVJ, 33, 571- 576.
  • 3. Apparicio M., Vicente WRR., Farias A., Covizzi GJ., Ribeiro APC., Gadelha CRF., 2007. Pyonephrosis following ovariohysterectomy in a bitch: case report. ARS Veterinaria, 23, 19-22.
  • 4. Konovalova MV., Markov PA., Popova GY., Nikitina IR., Shumikhin KV., Kurek DV., Varlamov VP., Popov SV., 2017. Prevention of postoperative adhesions by biodegradable cryogels from pectin and chitosan polysaccharides. J Bioact Compat Pol, 32, 1-16.
  • 5. Zheng Z., Zhang W., Sun W., Li X., Duan J., Cui J., Feng Z., Mansour HM., 2013. Influence of the carboxymethyl chitosan anti-adhesion solution on the TGF-b1 in a postoperative peritoneal adhesion rat. J Mater Sci: Mater Med, 24, 2549- 2559.
  • 6. Aguilar A., Zein N., Harmouch E., Hafdi B., Bornert F., Offner Damien., Clauss F., Fioretti F., Huck O., Benkirane-Jessel B., Hua G., 2019. Application of chitosan in bone and dental engineering. Molecules, 24, 1-17.
  • 7. Rodriguez DF., Mendes FF., Melo RM., Menezes LB., Guimaraes LLB., Silva AS., Rammazzina Filho WA., Moraes AM., Paulo NM., 2019. Polypropylene meshes coated with chitosan/polyethylene glycol for the reconstruction of the abdominal wall: an experimental study in rats. Arq Bras Med Vet Zootec, 71, 1198-1206.
  • 8. Yeo Y., Burdick JA., Highley CB., Marini R., Langer R., Kohane DS., 2006. Peritoneal application of chitosan and UV-cross-linkable chitosan. J Biomed Mater Res A, 78, 668-675.
  • 9. Furuhama K., Onodera T., 1983. A simple technique for repeated blood collection from the tail vein of the rat. J Toxicol Sci, 8, 161-163.
  • 10. Hooker GD., Taylor BM., Driman DK., 1999. Prevention of adhesion formation with use of sodium hyaluronate–based bioresorbable membrane in a rat model of ventral hernia repair with polypropylene mesh-A randomized, controlled study. Surgery, 125, 211-216.
  • 11. Lauder CIW., Garcea G., Strickland A., Maddern GJ., 2011. Use of a modified chitosane-dextran gel to prevent peritoneal adhesions in a rat model. J Surg Res, 171, 877-882.
  • 12. Garrard CL., Clements RH., Nanney L., Davidson JM., Richards WO., 1999. Adhesion formation is reduced after laparoscopic surgery. Surg Endosc, 13, 10-13.
  • 13. Kennedy R., Costain DJ., McAlister VC., Lee TDG., 1996. Prevention of experimental postoperative peritoneal adhesions by N,O-carboxymethyl chitosan. Surgery, 120, 866-870.
  • 14. Çetin M., Ak D., Duran B., Çetin A., Güvenal T., Yanar O., 2003. Use of methylene blue and N,Ocarboxymethyl chitosan to prevent postoperative adhesions in a rat uterine horn model. Fertility and Sterility, 80, 698-701.
  • 15. Costain DJ., Kennedy R., Ciona C., McAlister VC., Lee TD., 1997. Prevention of postsurgical adhesions with N,O-carboxymethyl chitosan: examination of the most efficacious preparation and the effect of N,O-carboxymethyl chitosan on postsurgical healing. Surgery, 121, 314-331.
  • 16. Chung YJ., An SY., Yeon JY., Shim WS., Mo JH., 2016. Effect of a chitosan gel on hemostasis and prevention of adhesion after endoscopic sinus surgery. Clin Exp Otorhinolaryngol, 9, 143-149.
  • 17. Krause TJ., Zazanis GA., McKinnon RD., 1996. Prevention of postoperative adhesions with the chitin derivative N-0-carboxymethylchitosan. Wound Rep Reg, 4, 53-57.
  • 18. Patrulea V., Ostafe V., Borchard G., Jordan O., 2015. Chitosan as a starting material for wound healing applications. Eur J Pharm Biopharm, 97, 417-426.
  • 19. Lin LX., Yuan F., Zhang HH., Liao NN., Luo JW, Sun YL., 2017. Evaluation of surgical anti-adhesion products to reduce postsurgical intra-abdominal adhesion formation in a rat model. PLOS ONE, 12, 1-9.
  • 20. Tian L., Li H., Li Y., Liu K., Sun Y., Cong Z., Luan X., Li Y., Chen J., Wang L., Ren Z., Cong D., Wang H., Pei J., 2018. A combination of chitosan, cellulose, and seaweed polysaccharide inhibits postoperative intra-abdominal adhesion in rats. J Pharmacol Exp Ther, 364, 399-408.
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Semra Kaya

Ekin Emre Erkılıç 0000-0003-2461-5598

Ali Haydar Kırmızıgül 0000-0002-6660-2149

Cihan Kaçar 0000-0002-2642-697X

Erdoğan Uzlu 0000-0002-3064-6633

Tuncay Tufan 0000-0001-8420-4235

Yayımlanma Tarihi 26 Nisan 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 16 Sayı: 1

Kaynak Göster

APA Kaya, S., Erkılıç, E. E., Kırmızıgül, A. H., Kaçar, C., vd. (2021). Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16(1), 41-48.
AMA Kaya S, Erkılıç EE, Kırmızıgül AH, Kaçar C, Uzlu E, Tufan T. Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. Nisan 2021;16(1):41-48.
Chicago Kaya, Semra, Ekin Emre Erkılıç, Ali Haydar Kırmızıgül, Cihan Kaçar, Erdoğan Uzlu, ve Tuncay Tufan. “Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16, sy. 1 (Nisan 2021): 41-48.
EndNote Kaya S, Erkılıç EE, Kırmızıgül AH, Kaçar C, Uzlu E, Tufan T (01 Nisan 2021) Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16 1 41–48.
IEEE S. Kaya, E. E. Erkılıç, A. H. Kırmızıgül, C. Kaçar, E. Uzlu, ve T. Tufan, “Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 16, sy. 1, ss. 41–48, 2021.
ISNAD Kaya, Semra vd. “Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16/1 (Nisan 2021), 41-48.
JAMA Kaya S, Erkılıç EE, Kırmızıgül AH, Kaçar C, Uzlu E, Tufan T. Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021;16:41–48.
MLA Kaya, Semra vd. “Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 16, sy. 1, 2021, ss. 41-48.
Vancouver Kaya S, Erkılıç EE, Kırmızıgül AH, Kaçar C, Uzlu E, Tufan T. Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan’ın Postoperatif Peritoneal Adezyon Üzerine Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021;16(1):41-8.