It was aimed to evaluate the regressive effect of grayanotoxin-rich Turkish mad honey and 5-fluorouracil (5-FU), separately and together by using the N-methyl-N-nitrosourea (MNU)-induced colon cancer modelling in rats. Study groups were designed as control group (CG), cancer control group (CCG), 5-Flourouracil group (FUG), Turkish mad honey group (HG), Turkish mad honey and 5-FU combined group (FU-HG). White blood cell (WBC), lymphocyte, eosinophil, basophil, serum lactate dehydrogenase (LDH), total oxidant status (TOS), and total protein values of the rats in the CCG were significantly lower than the values of the rats in the CG, whereas serum Bcl-2 and survivin levels were significantly higher in the rates belonged to the CCG in comparison to those in the CG. The presence of anaplastic epithelial cells, vascularization, precancerous changes, and inflammatory infiltration detected in the colon and small intestine of the rats in FU-HG, FUG, HG were less intense (P<0.05) compared to the findings in the rats in CCG. In conclusion, mad honey and 5-FU reduced anaplastic cell growth and oxidative stress via suppressed anti-apoptotic activity. Considering the histopathological findings in the liver and kidney, no toxicity occurred related to mad honey and 5-FU metabolization. Therefore, the combined use of these two substances may be an alternative method in the treatment of colon cancer.
Permission to conduct the study was granted by the ethics committee decision numbered 2020-7-55 of Ankara University Animal Experiments Local Ethics Committee, Türkiye.
This study was supported by Ankara University Scientific Research Projects Coordinatorship Grant No: 21Ö0239001.
Grant No: 21Ö0239001
The study is not produced from a thesis. This research article presented in 4th International Conference On Natural Products for Cancer Prevention and Therapy, 18-19 December 2021, Kayseri/Türkiye.
Grant No: 21Ö0239001
Birincil Dil | İngilizce |
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Konular | Veteriner Biyokimya, Veteriner Patoloji, Zootekni, Genetik ve Biyoistatistik |
Bölüm | Araştırma Makalesi |
Yazarlar | |
Proje Numarası | Grant No: 21Ö0239001 |
Yayımlanma Tarihi | 29 Eylül 2023 |
Yayımlandığı Sayı | Yıl 2023 |