Serotyping, genetic characterization and antimicrobial susceptibility determination ofVibrio anguillarum strains isolated from farmed rainbow trout (Oncorhynchus mykiss) in the easternBlack Sea
Öz
Antibiotic resistance profile, serotyping and identification of Vibrio anguillarum isolated from rainbow trout (Oncorhynchus mykiss) cultured in cages in the Eastern Black Sea Region of Turkey, were performed in the study. The biochemical, serological and molecular techniques were used in identification of V. anguillarum isolates. The sequenced isolates were confirmed to be similar to V. anguillarum which stored under different accession numbers in GenBank database. The rate of 97-99% similarity were found among the compared isolates. All isolates were identified to be serotype O1 by slide agglutination test results. The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol
Anahtar Kelimeler
Antimicrobial sensitivity diagnostic PCR rainbow trout Vibrio anguillarum
Kaynakça
- Austin B, Austin DA (1987): Bacterial Fish Pathogens: Disease of farmed and wild fish. Ellis Horwood Ltd., Chichester, UK.
- Austin B, Austin DA (1999): Bacterial Fish Pathogens: Disease of farmed and wild fish. Springer-Praxis Publishing, Chichester, UK.
- Austin B, Austin DA (2007): Bacterial Fish Pathogens: Diseases of farmed and wild fish. Praxis Publishing Chichester, UK.
- Austin B, Austin DA (2012): Bacterial Fish Pathogens: Disease of farmed and wild fish. Springer, New York.
- Avsever ML, Ün C (2015): Distribution of hemolysin genes in Turkish Vibrio anguillarum isolates. Bull Eur Ass Fish Pathol, 35, 75-84.
- Balta F, Sandalli C, Kayis S ve ark. (2010): Molecular analysis of antimicrobial resistance in Yersinia ruckeri strains isolated from rainbow trout (Oncorhynchus mykiss) grown in commercialfish farms in Turkey. Bull Eur Ass Fish Pathol, 30, 211-219.
- Candan A (2000): Türkiye’de üretilen Atlantik salmonu (Salmo salar l.)’nda tespit edilen ilk vibriosis olgusu. Türk Mikrobiyol Cem Derg, 30, 107-108.
- CLSI (2014): Performance standards for antimicrobial susceptibility Supplement. Clinical Laboratory Standards Institute, Wayne, USA, M100-S24, 230s. Informational
- Çağırgan H (2004): Vaccine development in sea bass fry (Dicentrarchus labrax L., 1758) against vibriosis. EU Su Ürünleri Dergisi, 21, 271-274.
- Demircan D, Candan A (2006): Identification of Vibrio anguillarum by PCR (rpoN gene) associated with vibriosis in marine fish in Turkey. Turk J Vet Anim Sci, 30, 305-310.
- Drancourt M, Bollet C, Carlioz A ve ark. (2000): 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol, 38, 3623-3630.
- Frans I, Michiels CW, Bossier P ve ark. (2011): Vibrio anguillarum as a fish pathogen: Virulence factors, diagnosis and prevention. J Fish Dis, 34, 643-661.
- Grisez L, Ceusters R, Oliever F (1991): The use of API 20E for the identification of Vibrio anguillarum and V. ordalii. J Fish Dis, 14, 359-365.
- Grisez L, Oliever F (1995): Comparative serology of the marine fish pathogen Vibrio anguillarum. Appl Environ Microbiol, 61, 4367-4373.
- Kitao T, Aoki T, Fukudome M ve ark. (1983): Serotyping of Vibrio anguillarum isolated from diseased fresh water fish in Japan. J Fish Dis, 6, 175-181.
- Korun J (2006): Kültürü yapılan çipuralarda (Sparus aurata L.) görülen Listonella anguillarum enfeksiyonu üzerine bir çalışma. EU Su Ürünleri Dergisi, 23, 259-263.
- Korun J, Gokoglu M (2007): Listonella anguillarum isolated from hatchery cultured red porgy Pagrus pagrus in Turkey. J Anim Vet Adv, 6, 823-827.
- MacDonell MT, Colwell RR (1985): Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol, 6, 171- 182.
- Maugeri TL, Caccamo D, Gugliandolo C (2000): Potentially pathogenic vibrios in brackish waters and mussels. J Appl Microbiol, 89, 261-266.
- Maugeri TL, Crisafi E, Genovese L ve ark. (1983): Identification of Vibrio anguillarum with the API 20 E system. Microbiologica, 1, 73-79.
- Pedersen K, Grisez L, van Houdt R ve ark. (1999): Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O- serogroups. Curr Microbiol, 38, 183-189.
- Popovic NT, Coz-Rakovac R, Strunjak-Petrovic I (2007): Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: A review. Veterinarni Medicina, 52, 49-53.
- Savaş H, Türe M (2007): Bölgemizde doğal ve kültürü yapılan balıklarda görülen hastalıklar. SUMAE Yunus Araştırma Bülteni, 7, 10-13.
- Sİrensen UBS, Larsen JL (1986): Serotyping of Vibrio anguillarum. Appl Environ Microbiol, 51, 593-597.
- Tanrikul TT (2007): Vibriosis as an epizootic disease of rainbow trout (Onchorhynchus mykiss) in Turkey. Pak J Biol Sci, 10, 1733-1737.
- Tanrikul TT, Cagirgan H, Toksen E (2004): Levreklerden (Dicentrarchus labrax L., 1758) izole edilen vibrio türlerinin API 20E yöntemiyle identifikasyonu. EÜ Su Ürünleri Dergisi, 21, 243-247.
- Tanrikul TT, Gultepe N (2011): Mix infections in rainbow Lactococcus garvieae and Vibrio anguillarum O1. J Anim Vet Adv, 10, 1019-1023. mykiss, Walbaum)
- Toranzo AE, Barja JL (1990): A review of the taxonomy and sero epizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain. Dis Aquat Org, 9, 73-82.
- Toranzo AE, Baya AM, Roberson BS ve ark. (1987): Specificity of slide aglutination test for detection bacterial fish pathogens. Aquaculture, 61, 81-97.
- Toranzo AE, Santos Y, Barja JL (1997): Immunization with bacterial antigens: Vibrio infections. Dev Biol Stand, 90, 93-105.
- Urakawa H, Kita-Tsukamoto K, Ohwada K (1997): 16s rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae. FEMS Microbiol Lett, 152, 125-132.
- Vaseeharan B, Ramasamy P, Murugan T ve ark. (2005): In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. Int J Antimicrob Agents, 26, 285-291.
- Weisburg WG, Barns SM, Pelletier DA ve ark. (1991): 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol, 173, 697-703.
Doğu Karadeniz’de yetiştiriciliği yapılan gökkuşağı alabalıkları(Oncorhynchus mykiss)’ndan izole edilen Vibrio anguillarum suşlarınınserotiplendirilmesi, genetik karakterizasyonu ve antimikrobiyalduyarlılığının belirlenmesi
Öz
Bu çalışmada, Türkiye’nin Doğu Karadeniz Bölgesi’nde kafeslerde yetiştirilen gökkuşağı alabalık (Oncorhynchus mykiss)’larından izole edilen Vibrio anguillarum suşlarının identifikasyonu ve serotiplendirilmesi yapıldı ve antimikrobiyal direnç profilleri belirlendi. V. anguillarum suşlarının tanımlanmasında biyokimyasal, serolojik ve moleküler teknikler kullanıldı. Dizi analizine gönderilen şuşlar GenBank veri tabanında farklı kabul numarasıyla kayıtlı olan V. anguillarum ile karşılaştırıldı. Karşılaştırma yapılan suşlar arasında %97-99 oranında benzerlik olduğu tespit edildi. Lam aglütinasyon test sonuçlarına göre, tüm izolatların serotip O1 olduğu belirlendi. Antimikrobiyal duyarlılık test sonuçlarına göre, izolatların sulphamethoxazole (%100), ampicilline (%90,6), eritromisine (%71,9), oksitetrasiklin (%62,5), streptomisin’e (% 46,9) ve trimetoprim+sulfametoksazol (%31,3) dirençli olduğu, fakat oksolinik asit, enrofloksasin ve florfenikole duyarlı olduğu tespit edildi
Anahtar Kelimeler
Antimikrobiyal duyarlılık gökkuşağı alabalığı PZR tanı Vibrio anguillarum
Kaynakça
- Austin B, Austin DA (1987): Bacterial Fish Pathogens: Disease of farmed and wild fish. Ellis Horwood Ltd., Chichester, UK.
- Austin B, Austin DA (1999): Bacterial Fish Pathogens: Disease of farmed and wild fish. Springer-Praxis Publishing, Chichester, UK.
- Austin B, Austin DA (2007): Bacterial Fish Pathogens: Diseases of farmed and wild fish. Praxis Publishing Chichester, UK.
- Austin B, Austin DA (2012): Bacterial Fish Pathogens: Disease of farmed and wild fish. Springer, New York.
- Avsever ML, Ün C (2015): Distribution of hemolysin genes in Turkish Vibrio anguillarum isolates. Bull Eur Ass Fish Pathol, 35, 75-84.
- Balta F, Sandalli C, Kayis S ve ark. (2010): Molecular analysis of antimicrobial resistance in Yersinia ruckeri strains isolated from rainbow trout (Oncorhynchus mykiss) grown in commercialfish farms in Turkey. Bull Eur Ass Fish Pathol, 30, 211-219.
- Candan A (2000): Türkiye’de üretilen Atlantik salmonu (Salmo salar l.)’nda tespit edilen ilk vibriosis olgusu. Türk Mikrobiyol Cem Derg, 30, 107-108.
- CLSI (2014): Performance standards for antimicrobial susceptibility Supplement. Clinical Laboratory Standards Institute, Wayne, USA, M100-S24, 230s. Informational
- Çağırgan H (2004): Vaccine development in sea bass fry (Dicentrarchus labrax L., 1758) against vibriosis. EU Su Ürünleri Dergisi, 21, 271-274.
- Demircan D, Candan A (2006): Identification of Vibrio anguillarum by PCR (rpoN gene) associated with vibriosis in marine fish in Turkey. Turk J Vet Anim Sci, 30, 305-310.
- Drancourt M, Bollet C, Carlioz A ve ark. (2000): 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol, 38, 3623-3630.
- Frans I, Michiels CW, Bossier P ve ark. (2011): Vibrio anguillarum as a fish pathogen: Virulence factors, diagnosis and prevention. J Fish Dis, 34, 643-661.
- Grisez L, Ceusters R, Oliever F (1991): The use of API 20E for the identification of Vibrio anguillarum and V. ordalii. J Fish Dis, 14, 359-365.
- Grisez L, Oliever F (1995): Comparative serology of the marine fish pathogen Vibrio anguillarum. Appl Environ Microbiol, 61, 4367-4373.
- Kitao T, Aoki T, Fukudome M ve ark. (1983): Serotyping of Vibrio anguillarum isolated from diseased fresh water fish in Japan. J Fish Dis, 6, 175-181.
- Korun J (2006): Kültürü yapılan çipuralarda (Sparus aurata L.) görülen Listonella anguillarum enfeksiyonu üzerine bir çalışma. EU Su Ürünleri Dergisi, 23, 259-263.
- Korun J, Gokoglu M (2007): Listonella anguillarum isolated from hatchery cultured red porgy Pagrus pagrus in Turkey. J Anim Vet Adv, 6, 823-827.
- MacDonell MT, Colwell RR (1985): Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol, 6, 171- 182.
- Maugeri TL, Caccamo D, Gugliandolo C (2000): Potentially pathogenic vibrios in brackish waters and mussels. J Appl Microbiol, 89, 261-266.
- Maugeri TL, Crisafi E, Genovese L ve ark. (1983): Identification of Vibrio anguillarum with the API 20 E system. Microbiologica, 1, 73-79.
- Pedersen K, Grisez L, van Houdt R ve ark. (1999): Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O- serogroups. Curr Microbiol, 38, 183-189.
- Popovic NT, Coz-Rakovac R, Strunjak-Petrovic I (2007): Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: A review. Veterinarni Medicina, 52, 49-53.
- Savaş H, Türe M (2007): Bölgemizde doğal ve kültürü yapılan balıklarda görülen hastalıklar. SUMAE Yunus Araştırma Bülteni, 7, 10-13.
- Sİrensen UBS, Larsen JL (1986): Serotyping of Vibrio anguillarum. Appl Environ Microbiol, 51, 593-597.
- Tanrikul TT (2007): Vibriosis as an epizootic disease of rainbow trout (Onchorhynchus mykiss) in Turkey. Pak J Biol Sci, 10, 1733-1737.
- Tanrikul TT, Cagirgan H, Toksen E (2004): Levreklerden (Dicentrarchus labrax L., 1758) izole edilen vibrio türlerinin API 20E yöntemiyle identifikasyonu. EÜ Su Ürünleri Dergisi, 21, 243-247.
- Tanrikul TT, Gultepe N (2011): Mix infections in rainbow Lactococcus garvieae and Vibrio anguillarum O1. J Anim Vet Adv, 10, 1019-1023. mykiss, Walbaum)
- Toranzo AE, Barja JL (1990): A review of the taxonomy and sero epizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain. Dis Aquat Org, 9, 73-82.
- Toranzo AE, Baya AM, Roberson BS ve ark. (1987): Specificity of slide aglutination test for detection bacterial fish pathogens. Aquaculture, 61, 81-97.
- Toranzo AE, Santos Y, Barja JL (1997): Immunization with bacterial antigens: Vibrio infections. Dev Biol Stand, 90, 93-105.
- Urakawa H, Kita-Tsukamoto K, Ohwada K (1997): 16s rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae. FEMS Microbiol Lett, 152, 125-132.
- Vaseeharan B, Ramasamy P, Murugan T ve ark. (2005): In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. Int J Antimicrob Agents, 26, 285-291.
- Weisburg WG, Barns SM, Pelletier DA ve ark. (1991): 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol, 173, 697-703.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Diğer ID | JA39NH57SV |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 2017 |
| Yayımlandığı Sayı | Yıl 2017 |