Effects of boron (sodium pentaborate), added instead of Tris components, on freezing and post-thaw quality of Angora buck semen
Öz
In this research, we aimed to evaluate freezing and in vitro results of post-thawed Angora goat semen parameters using boron (sodium pentaborate, Na2O.5BO3.10H2O) instead of other components and as an additive in Tris extender. Mature Angora bucks, with proven fertility, accustomed to artificial vagina, and with normospermic values have been used in the study. Research was carried out with 9 different extenders: Boron extenders prepared by adding different amounts of Boron instead of glucose into standard Tris extender (GIB, 1-a and 1-b, 1-c, 1-d), the standard Tris extenders prepared by only addition of different amounts of Boron (BAD; 2-a, 2-b, 2-c, 2-d) and standard Tris extender (Control). Post-thaw spermatological examinations have been done with sperm analyzer (CASA) and results were recorded as follows; the highest total and progresive motility scores between 9 different extenders belonged to 1-c with 63,34 ± 2,32 and 1-d with %38,27± 0,74, highest VCL, VSL, VAP, LIN, STR and WOB average scores were found in 2-d extender (p<0,001).The percentage of lowest dead spermatozoa rate % 42,175± 1,59 was observed with GIB between main groups (p<0,01).Between extenders, lowest dead spermatozoa rates were recorded as % 36,04± 2,58 and % 36,17± 3,66 in 2-b and 1-c (p<0,001).When abnormal spermatozoa rates were examined, lowest abnormal spermatozoon rate belonged to 2-c extender with 21,29± 0,97 (p<0,001) and abnormal acrosome rates were obtained from %6,57± 1,29 1-a extender with (p<0,01). In conclusion, some positive effects of sodium pentaborate on post-thaw spermatological parameters of Angora buck semen have been demonstrated
Anahtar Kelimeler
Kaynakça
- Berlinguer F, Madeddu M, Pasciu V, Succu S, Spezzigu A, Satta V, Mereu P, Leoni GG, Naitana S (2009): Semen molecular and cellular features: these parameters can reliably predict subsequent ART outcome in a goat model. Reproductive Biology and Endocrinology, 7, 125.
- Bucak MN, Uysal O (2008): The role of antioxidants in freezing of Saanen goat semen. Indian Vet J, 85,148-150.
- Büyükleblebici S (2012): Farklı Sulandırıcılar ile dondurulan boğa spermasında İn Vitro ve İn Vivo Değerlendirmeler. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü.
- Daşkın A, Tekin N (1996): The effect of egg-yolk on the quality of frozen Angora buck semen. Tr J of Vet and Ani Sci, 20, 395-398.
- De Geyter C, De Geyter M, Koppers B, Nieschlag E (1998): Diagnostic accuracy of computer-assisted sperm motion analysis. Human Reproduction, 13, 2512-2520.
- Donnelly ET, Lewis SEM, Mcnally JA, Thompson W (1998): In vitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fertility and Sterility, 70, 305-314.
- Dorado J, Molına I, Munoz-Serrano A, Hidalgo M (2010): Identification of sperm subpopulations with defined motility characteristics in ejaculates from Florida goats. Theriogenology, 74, 795-804.
- Espinoza-Navarro O, Vilaxa A, Granifo L, Rojas S, Rodriguez H (2007): Histological study on the male reproductive organs of mouse CF1 treated with Boron.Int J Morphol, 25, 341-346.
- Farrel PB, Presicce GA, Brockett CC, Foote RH (1998): Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology, 49, 871-879.
- Garcia-Macias V, De Paz P, Martinez-Pastor F, Alvarez M, Gomes-Alves S, Bernardo J, Anel E, Anel L (2007): DNA fragmentation assessment by flowcytometry and sperm-bos-halamox (bright-field microscopy and floresence microscopy) in bull sperm. Journal of Andrology, 30, 88-98.
- Ince S, Kucukkurt I, Cigerci IH, Fidan AF, Eryavuz A (2010): The effects of dietary Boric Acid and Borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. Journal of Trace Elements in Medicine and Biology, 24, 161-164.
- Januskauskas A, Johannisson A, Rodriguez-Martinez H (2003): Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure and field fertility. Theriogenology, 60, 743-758.
- Januskauskas A, Zillinskas H (2002):Bull semen evaluation postthaw and relation of semen charecteristics to bull’s fertility. Veterinarija ir zootechnika, 17, 1-8.
- Johnston SD, Lopez-Fernandez C, Gosalbez A, Zee Y, Holt WV, Allen C, Gosalvez J (2007): The relationship between sperm morphology and chromatin ıntegrity in the Koala (phascolarctos cinereus) as assessed by the sperm chromatin dispersion test (scdt). Journal of Andrology, 28, 6.
- Ku WW, Chapin RE, Moseman RF, Brink RE, Pierce KD, Adams KY (1991): Tissue distribution of boron in male fischer rats. Toxicol Appl Pharmacol, 111, 145-151.
- Ku WW, Chapin RE, Wine RN, Gladen BC (1993): Testicular toxicity of boric acid: relationship of dose to lesion development and lack of recovery in the f344 rat. Reprod Toxicol, 7, 305-319.
- Kulaksız R, Çebi Ç, Akçay E, Daşkın A (2010): The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Rum Res, 88, 12-15.
- Kulaksız R, Daşkın A (2010): In vitro evaluation of saanen buck semen frozen in different extenders supplemented with various antioxidants. Ankara Üniv Vet Fak Derg, 57, 151-156.
- Ritar AJ, Mendoza G, Salamon, S, White IG (1992): Frequent semen collection and sperm reserves of the male Angora Goat (Capra Hircus). J Reprod Fert, 95, 97-102.
- Robayo I, Montenegro V, Valdes C, Cox JF (2008): CASA assessment of kinematic parameters of ram spermatozoa and their relationship to migration efficiency in ruminant cervical mucus. Reprod Dom Anim, 43, 393- 399.
- Ruiz-Pessini E, Alvarez E, Enriquez J, Lopez-Perez M (2001): Association between seminal plasma carnitine and sperm mitochondrial enzymatic activities. Int J And, 24, 335-340.
- Sevinç A, Tekin N, Yurdaydın N, Kale N (1985): Çifteler harası tiftik tekelerinin başlıca spermatolojik özellikleri üzerinde araştırmalar. Doğa Bilim Derg, 9, 265-273.
- Tekin K (2011): İki farklı sulandırıcı ile dondurulan Norduz tekesi spermasının motilite, vap, vcl, vsl parametreleri üzerinden değerlendirilmesi. Yüksek Lisans Tezi, Ankara Üniveresitesi Sağlık Bilimleri Enstitüsü.
- Tekin N, Günzel-Apel AR, Yurdaydın N, Yavaş Y, Daşkın A, Keskin O, Etem H (1991): Östrusları sinkronize edilen koyunlarda suni tohumlama yöntemiyle elde edilen dölverimi. Ankara Üniv Vet Fak Derg, 38, 60-73.
- Tekin N, Yurdaydın N, Daşkın A (1996): Ankara keçilerinden değişik yöntemlerle sperma alınması ve değerlendirilmesi. Ankara Üniv Vet Fak Derg, 43, 397-403.
- Turkez H, Geyıkoglu F, Tatar A, Keles S, Ozkan A (2007): Effects of some Boron compounds on peripheral human blood. Z Naturforsch, 62, 889-896.
- Van der Horst G, Maree L (2009): SpermBlue: a new universal stain for human and animal sperm which is also amenable to automated sperm morphology analysis. Biotech Histochem, 84, 299-308.
Ankara tekesi spermasının dondurulmasında Tris sulandırıcı bileşenleri yerine katilan borun (sodyumpentaborat) çözüm sonu sperma kalitesi üzerine etkisi
Öz
Bu araştırmada, Ankara tekesi spermasının dondurulması ve çözdürülmesinde, borun (sodyum pentaborat, Na2O.5B2O3.10H2O) Tris sulandırıcısına eklenerek ve bileşenleri yerine katılarak hazırlanmış solüsyonlarla, elde edilecek in vitro sonuçların değerlendirilmesi amaçlandı. Çalışma materyali olarak, dölverimi kanıtlanmış ve suni vaginaya sperma vermeye alıştırılmış ergin Ankara tekelerinin normospermik ejakülatları alındı. Çalışmada standart Tris sulandırıcısından glikoz çıkarılıp farklı miktarlarda bor eklenerek hazırlanmış sulandırıcılar (GYB; 1-a, 1-b, 1-c, 1-d), standart Tris sulandırıcısına farklı miktarlarda bor eklenerek hazırlanmış sulandırıcılar (BEK;2-a, 2-b, 2-c, 2-d) ve standart Tris sulandırıcısı (Kontrol) olmak üzere 9 farklı sulandırıcı kullanıldı. Sperma analiz sistemi (CASA) ile total motilite ve progresif motilite değerlerine bakıldığında sırasıyla en yüksek değerinlerin 63,34 ± 2,32 ile 1-c ve %38,27± 0,74 ile 1-d sulandırıcılarına VCL, VSL, VAP, LIN, STR, WOB verilerinin en yüksek 2-d sulandırıcısına ait olduğu belirlendi (p<0,001). Ölü spermatozoon oranı değerlendirildiğinde en düşük ölü spermatozoon oranının % 36,04± 2,58 ve % 36,17± 3,66 ile sırasıyla 2-b ve 1-c sulandırıcılarına ait olduğu gözlemlendi (p<0,001). Anormal spermatozoon oranları incelendiğinde GYB %27,60± 0,79, BEK % 27,89± 1,36 olarak bulundu (p<0,05). Akrozoma bağlı anormal spermatozoon değerleri ise en düşük oran %21,29± 0,97 ile 2-c sulandırıcısından elde edildi (p<0,001). Akrozoma bağlı anormal spermatozoon verilerinde ise en düşük oranın ise %6,57± 1,29 ile 1-a sulandırıcısına ait olduğu belirlendi (p<0,01). Sonuç olarak sodyum pentaborat’ın Ankara tekesi çözüm sonu spermatolojik parametrelere olan olumlu bazı etkileri ortaya konulmuştur
Anahtar Kelimeler
Kaynakça
- Berlinguer F, Madeddu M, Pasciu V, Succu S, Spezzigu A, Satta V, Mereu P, Leoni GG, Naitana S (2009): Semen molecular and cellular features: these parameters can reliably predict subsequent ART outcome in a goat model. Reproductive Biology and Endocrinology, 7, 125.
- Bucak MN, Uysal O (2008): The role of antioxidants in freezing of Saanen goat semen. Indian Vet J, 85,148-150.
- Büyükleblebici S (2012): Farklı Sulandırıcılar ile dondurulan boğa spermasında İn Vitro ve İn Vivo Değerlendirmeler. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü.
- Daşkın A, Tekin N (1996): The effect of egg-yolk on the quality of frozen Angora buck semen. Tr J of Vet and Ani Sci, 20, 395-398.
- De Geyter C, De Geyter M, Koppers B, Nieschlag E (1998): Diagnostic accuracy of computer-assisted sperm motion analysis. Human Reproduction, 13, 2512-2520.
- Donnelly ET, Lewis SEM, Mcnally JA, Thompson W (1998): In vitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fertility and Sterility, 70, 305-314.
- Dorado J, Molına I, Munoz-Serrano A, Hidalgo M (2010): Identification of sperm subpopulations with defined motility characteristics in ejaculates from Florida goats. Theriogenology, 74, 795-804.
- Espinoza-Navarro O, Vilaxa A, Granifo L, Rojas S, Rodriguez H (2007): Histological study on the male reproductive organs of mouse CF1 treated with Boron.Int J Morphol, 25, 341-346.
- Farrel PB, Presicce GA, Brockett CC, Foote RH (1998): Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology, 49, 871-879.
- Garcia-Macias V, De Paz P, Martinez-Pastor F, Alvarez M, Gomes-Alves S, Bernardo J, Anel E, Anel L (2007): DNA fragmentation assessment by flowcytometry and sperm-bos-halamox (bright-field microscopy and floresence microscopy) in bull sperm. Journal of Andrology, 30, 88-98.
- Ince S, Kucukkurt I, Cigerci IH, Fidan AF, Eryavuz A (2010): The effects of dietary Boric Acid and Borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. Journal of Trace Elements in Medicine and Biology, 24, 161-164.
- Januskauskas A, Johannisson A, Rodriguez-Martinez H (2003): Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure and field fertility. Theriogenology, 60, 743-758.
- Januskauskas A, Zillinskas H (2002):Bull semen evaluation postthaw and relation of semen charecteristics to bull’s fertility. Veterinarija ir zootechnika, 17, 1-8.
- Johnston SD, Lopez-Fernandez C, Gosalbez A, Zee Y, Holt WV, Allen C, Gosalvez J (2007): The relationship between sperm morphology and chromatin ıntegrity in the Koala (phascolarctos cinereus) as assessed by the sperm chromatin dispersion test (scdt). Journal of Andrology, 28, 6.
- Ku WW, Chapin RE, Moseman RF, Brink RE, Pierce KD, Adams KY (1991): Tissue distribution of boron in male fischer rats. Toxicol Appl Pharmacol, 111, 145-151.
- Ku WW, Chapin RE, Wine RN, Gladen BC (1993): Testicular toxicity of boric acid: relationship of dose to lesion development and lack of recovery in the f344 rat. Reprod Toxicol, 7, 305-319.
- Kulaksız R, Çebi Ç, Akçay E, Daşkın A (2010): The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Rum Res, 88, 12-15.
- Kulaksız R, Daşkın A (2010): In vitro evaluation of saanen buck semen frozen in different extenders supplemented with various antioxidants. Ankara Üniv Vet Fak Derg, 57, 151-156.
- Ritar AJ, Mendoza G, Salamon, S, White IG (1992): Frequent semen collection and sperm reserves of the male Angora Goat (Capra Hircus). J Reprod Fert, 95, 97-102.
- Robayo I, Montenegro V, Valdes C, Cox JF (2008): CASA assessment of kinematic parameters of ram spermatozoa and their relationship to migration efficiency in ruminant cervical mucus. Reprod Dom Anim, 43, 393- 399.
- Ruiz-Pessini E, Alvarez E, Enriquez J, Lopez-Perez M (2001): Association between seminal plasma carnitine and sperm mitochondrial enzymatic activities. Int J And, 24, 335-340.
- Sevinç A, Tekin N, Yurdaydın N, Kale N (1985): Çifteler harası tiftik tekelerinin başlıca spermatolojik özellikleri üzerinde araştırmalar. Doğa Bilim Derg, 9, 265-273.
- Tekin K (2011): İki farklı sulandırıcı ile dondurulan Norduz tekesi spermasının motilite, vap, vcl, vsl parametreleri üzerinden değerlendirilmesi. Yüksek Lisans Tezi, Ankara Üniveresitesi Sağlık Bilimleri Enstitüsü.
- Tekin N, Günzel-Apel AR, Yurdaydın N, Yavaş Y, Daşkın A, Keskin O, Etem H (1991): Östrusları sinkronize edilen koyunlarda suni tohumlama yöntemiyle elde edilen dölverimi. Ankara Üniv Vet Fak Derg, 38, 60-73.
- Tekin N, Yurdaydın N, Daşkın A (1996): Ankara keçilerinden değişik yöntemlerle sperma alınması ve değerlendirilmesi. Ankara Üniv Vet Fak Derg, 43, 397-403.
- Turkez H, Geyıkoglu F, Tatar A, Keles S, Ozkan A (2007): Effects of some Boron compounds on peripheral human blood. Z Naturforsch, 62, 889-896.
- Van der Horst G, Maree L (2009): SpermBlue: a new universal stain for human and animal sperm which is also amenable to automated sperm morphology analysis. Biotech Histochem, 84, 299-308.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Diğer ID | JA34DY37ZA |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 2015 |
| Yayımlandığı Sayı | Yıl 2015 |