Ankara Üniversitesi Veteriner Fakültesi Dergisi

Öz

This study was carried out to determine the distribution ol' eosinophil granulocytes and plasma cells in the jejunal mucosa (~f the dogs naturally
inl'ected or not infected with intestinal parasites. /n addition, the total leukocyte
and eosinophil granulocyte concentrations were determined in peripheral blood.
For this pUlposes, II mongrel dogs were used where 7 ol'them were determined to
be inl'ected naturally with in testin al parasites, and in 4 of them there were no evidence ol'an actual intestinal parasitic inl'ection.
The tissue specimens ol' the jejunum were obtained by abdominal surgery
fi"om both groups. Specimens were fixed with i 0% neutral bu.ti'eredformalin. The
tissue sections were stained with either Congo red for eosinofil granulocytes or
methyl green pyroninfor plasma cells. Tissue sections were examined by light microscopically. Both cells were counted in villus-crypt units olJejunal mucosa. The
determination ol' the leukocyte and eosinophil granulocyte concentrations in peripheral blood were carried outfrom blood samples with standart technics.
/t was observed that eosinophil granulocytes were located in lamina propria
ofjejunal mucosa, especialfy within vilfus intestinalis. basal parts of crypts and lamina subglandularis. FUl1hermore. eosinophil granulocytes were also located intraepithelially in crypts and rarely at the base of the villus. Some eosinophil granulocytes were also seen within intestinal lumen. The pyroninophilic cells were
identified in jejunum. They were intensively prominent in villus intestinales, but
they werefound also in crypts in a lesser extent.
A t-Testfor independent groups showed that the eosinophil granulocyte count
in jejunal mucosa was higher in parasitized dogs than in jejunal mucosa ol' noninl'ected dogs (p< 0.05). But there were no sign(ficant differences in CO/1-
centrations (JI' leukocytes and eosinophil granulocytes in peripheral blood, as well
as in the number ofplasma cells in jejunal mucosa when compared the dogs with
intestinal parasite with those showing no parasite infeces.