ankara univ vet fak dergAnkara Üniversitesi Veteriner Fakültesi Dergisi1300-08611308-2817Ankara ÜniversitesiMesothelioma in a dogKöpekte mezotelioma olgusuTerimKübra A. KapakinHazıroğluRıfkıGürsanNesrinYücelGözde10.1501/Vetfak_0000002518Veterinary SurgeryVeteriner Cerrahi0601201259215115306012012In this study a mesothelioma case observed in a four–year-old female Rottweiler dog was defined. Macroscopically, on the serosal surfaces of the stomach and intestines, numerous masses showing diffuse distribution, greyish yellow in colour and hard consistency were observed. Microscopically, tumour cells were predominantly spindle-shaped with moderate amounts of eosinophilic cytoplasm and ovoid nuclei. These cells was not stained with epithelial membrane antigen (EMA) staining, the epithelial marker, while stained with the epithelial marker pancytokeratin and mesenchymal marker vimentin in the avidin-biotin-peroxidase (ABC-P) method. As a result, tumor case seen in a dog's stomach and intestinal serosal surface was defined as a mesothelioma according to the histopathological and immunohistochemical findingsBu çalışmada 4 yaşlı Rottweiler ırkı, dişi bir köpekte gözlenen mezotelioma olgusu tanımlandı. Makroskobik olarak mide ve bağırsakların serozal yüzeyinde diffuz dağılım gösteren, değişik büyüklükte ve sert kıvamda, grimsi sarı renkte kitleler görüldü. Mikroskobik olarak tümör hücreleri çoğunlukla iğ şeklinde, eozinofilik sitoplazmalı ovoid çekirdekliydi. Bu hücreler uygulanan avidin biyotin peroksidaz (ABC-P) immunohistokimyasal boyama yönteminde, epiteliyal belirteç pansitokeratin ve mezenkimal belirteç vimentin ile boyanırken epiteliyal belirteç olan epitheliyal membran antijeni (EMA) ile boyanmadı. Sonuç olarak bir köpeğin mide ve bağırsak serozal yüzeyinde karşılaşılan tümör olgusu, histopatolojik ve immunohistokimyasal bulgularına göre mesotelioma olarak tanımlandıDog immunohistochemistry mesotheliomaİmmunohistokimya köpek mezoteliomaAmaya JO, Arevalo GH (2008): Invasive Mesothelioma of Canine: Cytology, Clinical and Pathology’s Findings. Int J Morphol, 1, 103-112Attanoos RL, Dojcinov SD, Webb R, Gibbs AR (2000): Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology, 37, 224–231.Avakian A, Alroy J, Rozanski E, Keating J, Rosenberg A (2008): Lipid-rich pleural mesothelioma in a dog. J Vet Diagn Invest, 5, 665-667.Bacci B, Morandi F, De Meo M, Marcato PS (2006): Ten cases of feline mesothelioma: an immunohistochemical and ultrastructural study. J Comp Pathol, 134, 347–354.Bollo E, Scaglione FE, Tursi . M, Schröder C, Degiorgi G, Belluso E, Capella S, Bellis D (2010): Malignant pleural mesothelioma in a female Lion (Panthera leo) Res Vet Sci, doi:10.1016/ jrvsc. 2010.08.005Cacciotti P, Libener R, Betta P, Martini F, Porta C, Procopio A, Strizzi L, Penengo L, Tognon M, Mutti L, Gaudino G (2001): SV40 replication in human mesothelial cells induces HGF/Met receptor activation: a model for viral-related carcinogenesis of human malignant mesotelioma. Proc Nati Acad Sci, 98, 12032- 12037.Caswell J, Wiliams K (2007): Respiratory system M. Maxie (ed) 5th (Ed), Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals, 5th edition, Elsevier, New York, NY, USA, p. 523–655.Croft W (1983): Environmental asbestos and mesotheliomas in dairy calves. Proc Am Assoc Cancer Res, 24, 188.Gibbs GW, Berry G (2008): Mesothelioma and asbestos. Regul Toxicol Pharmacol, 52, 223–31.Head KW, Else RW, Dubielzig RR (2002): Tumours of the alimentary tract. D.J. Meuten (ed.) Tumours In Domestic Animals, p. 401-481.Kim JH, Choi YK, Yoon HY, Kweon OK, Kim DY (2002): Juvenile malignant mesotelioma in a dog. J Vet Med Sci, 64, 269-271.Ledecka K, Sevcikova Z, Mihaly M, Hajurka J, Pavuk V, Hluchy M, Skurkova L, Lackova M, Ledecky V (2010) Mesothelioma of the pericardium in a Bernese Mesothelioma of the pericardium in a Bernese mountain dog. Veterinarski Arhıv, 80, 797-806.Presnell JK, Schreibman MP (1997): Humason’s Animal Tissue Techniques.. In: Presnell J., Schreibman M.P (ed.), 5th (Ed), The Johns Hopkins University Press. Ltd, London, p. 269-312.Vural SA, Ozyildiz Z, Ozsoy SY (2007): Pleural mesothelioma in a nine month-old dog. Irish Vet J, 1, 30- 33.Yamate J, Tomita A, Kuwamura M, Mitsunaga F, Nakamura S (2007): Spontaneous peritoneal malignant mesothelioma in a geriatric japanese macaque (Macaca fuscata). Exp Anim, 56, 155–159.