ankara univ vet fak dergAnkara Üniversitesi Veteriner Fakültesi Dergisi1300-08611308-2817Ankara ÜniversitesiThe effect of melatonin in rats with uterine torsion on uterus contractions, and the levels of ADMA, SDMA, arginine, Hsp90, TLR4, and NF-κBTorsiyo uterili ratlarda melatoninin uterus kontraksiyonları, ADMA, SDMA, arjinin, Hsp90, TLR4 ve NFκB düzeylerine etkisiDoğanHalefhttps://orcid.org/0000-0003-1365-1729RişvanlıAlihttps://orcid.org/0000-0001-5653-0025SaatNevzathttps://orcid.org/0000-0002-8135-6142GülHüseyin Fatihhttps://orcid.org/0000-0002-9828-1298İlhanNecibhttps://orcid.org/0000-0001-9997-0418Şekerİbrahimhttps://orcid.org/0000-0002-4791-2993ŞahnaEnginhttps://orcid.org/0000-0001-8311-905510.33988/auvfd.467062Veterinary SurgeryVeteriner Cerrahi061420196632672721003201805222019In this study was aimed at reducing uterine damage and increasing fertility after uterine torsion in pregnant animals. With this aim, uterine torsion was experimentally formed in 35 rats that were between 18-19 days pregnant. The animals were randomly divided into five groups, and melatonin was administered prior to torsion, at the time of torsion, and detorsion (10 mg/kg/gün IP). Ovario-hysterectomy operation was performed on all animals on the first day following parturition. Subsequently, from the obtained uterus samples, determination of the levels of asymmetrical dimethyl arginin (ADMA), symmetrical dimethyl arginin (SDMA), and arginine was made using the high-performance liquid chromatography (HPLC) and levels of Heat shock protein 90 (Hsp90), TLR4 (Toll Like Receptor 4) and NFκB (Nuclear factor kappa-light-chain-enhancer of activated B cells) were measured using the Western blot technique. The contraction-relaxation responses of the myometrium were also determined in the organ baths. According to the results of the western blot, higher protein expressions than those of the control group were determined in the second, third, fourth, and fifth groups in Hsp90, TLR4, NF-κB. The lowest values of arginine and ADMA were found in Group 3, whilst the lowest SDMA value was determined in Group 1. It was determined that melatonin reduces tissue damage secondary to torsio uteri and, furthermore, that administration of this hormone at the time of torsion formation was more effective than its administration at the time of detorsion.Bu çalışmada gebe hayvanlarda torsiyo uteri sonrası şekillenen uterus hasarını azaltmaya ve fertiliteyi artırmaya yönelik bir çalışma planlandı. Bu amaçla 35 adet 18-19 günlük gebe ratda deneysel olarak torsiyo uteri oluşturuldu. Hayvanlar rastgele 5 gruba ayrılarak torsiyon öncesi, torsiyon anında ve detorsiyon sırasında melatonin (10 mg/kg/gün IP) uygulandı. Doğum sonrası ilk gün tüm hayvanlara ovariohisterektomi operasyonu yapılarak kornulardan numuneler alındı. Daha sonra elde edilen uterus dokularında asymmetrical dimethyl arginin (ADMA), symmetrical dimethyl arginin (SDMA) ve Arjinin miktar tayinleri yüksek performanslı sıvı kromatografisi (HPLC) ve Heat shock protein 90 (Hsp90), TLR4 (Toll Like Receptor 4) ve NFκB (Nuclear factor kappa-light-chainenhancer of activated B cells) düzeyleri ise Western Blot ile belirlendi. Miyometriyumun in vitro kasılma gevşeme cevapları da organ banyosunda tespit edildi. Western blot sonuçlarına göre HSP90, TLR4, NFκB’da 2, 3, 4 ve 5. gruplarda kontrol grubuna göre daha fazla protein ekspresyonu olduğu belirlendi. Arjinin ve ADMA değerleri en düşük olarak 3. grupta bulundu. En düşük SDMA değeri ise 1. grupta tespit edildi. 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