ankara univ vet fak dergAnkara Üniversitesi Veteriner Fakültesi Dergisi1300-08611308-2817Ankara ÜniversitesiThe role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin IIIKeleşHikmetBozkurtMehmet FatihUlucanAykutDemirelHasan HüseyinYükselHayatiKaradaşErkanÖzkulAyhan10.1501/Vetfak_0000002866Veterinary SurgeryVeteriner Cerrahi110920186543413480203201704162017Changes in uroplakin secretion in the urothelium leads a gradual ability lost in neoplastic conditions. Urothelium isone of the target regions where nitric oxide can damage. Therefore, in this study, the presence of iNOS and UPIII were investigated inbovine enzootic hematuria cases. The results were supported by comparing them with those achieved by critical markers for malignancyof tumor such as caspase 3, Bax, p53, PCNA, and VEGF. The presence of 8-OHDG, a good marker for oxidative DNA damage, wasalso investigated. For this purpose, urinary bladders with enzootic hematuria were collected from the slaughterhouses. Inhistopathological examinations, neoplastic, pre-neoplastic, and inflammatory lesions were observed in these urinary bladders. Inimmunohistochemical examinations, iNOS reacts as negative in the UPIII positive healthy urothelium which preserves the structure ofAUM. On the other hand positive reaction has been observed in AUM modified and UPIII negative urothelium. In these cases, as anindicator of oxidative damage, 8-OHdG positivity was observed in the UPIII negative and iNOS positive areas. Additionally, increasedcell proliferation, decreased apoptosis as well as p53 disorganization were clearly observed in most of the cases. Thus, the active roleof nitric oxide, as a free radical derivative, has been shown in the enzootic hematuria cases of cattle. Bovine enzootic hematuria iNOS nitric oxide urinary bladder uroplakin III1. Ambrosio V, Borzacchiello G, Bruno F, et al. (2001):
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