ankara univ vet fak dergAnkara Üniversitesi Veteriner Fakültesi Dergisi1300-08611308-2817Ankara ÜniversitesiThe immunohistochemical localization of the fibronectin receptor (Integrin β1) and laminin in the bitch uterus during the estrous cycleKüçük BayramGünerLimanNarinBeyazFeyzullahAlanEmel10.1501/Vetfak_0000002868Veterinary SurgeryVeteriner Cerrahi110920186543573640328201707032017In mammals, the purpose of the estrous cycle is the successful progression of implantation and pregnancy. Theendometrium undergoes several changes during the estrous cycle. The extracellular matrix proteins have a likely role in the maintenanceof a healthy endometrium. In this study, we investigated the localization of the fibronectin receptor (Integrin β1), a member of theintegrin family, and laminin, an extracellular matrix protein, in the bitch uterus throughout the estrous cycle. In the bitch uterus,immunoreactions of similar intensity were observed for the fibronectin receptor (Integrin β1) in the myometrium, perimetrium,peritoneal mesothelial cells and uterine vasculature, and for laminin in the peritoneal mesothelial cells, throughout the estrous cycle.Laminin and the fibronectin receptor (Integrin β1) were found to be present at high intensity in the endometrium of the bitch uterus atthe luteal stage. Bitch estrous cycle fibronectin laminin uterus1. Beijerink NJ (2007): Endocrinology of physiology and
progestin - induced canine anoestrus. PhD thesis, Faculty
of Veterinary Medicine, Utrecht University, The Netherlands.2. Beliard A, Dönmez J, Nisolle M, et al. (1997):
Localization of laminin, fibronectin, E-cadherin and
integrins in endometrium and endometriosis. Fertil Steril,
67, 266-272.3. Bilalis DA, Klentzeris LD, Fleming S (1996):
Immunohistochemical localization of extracellular matrix
proteins in luteal phase endometrium of fertile and infertile
patients. Hum Reprod, 11, 2713-2718.4. Bowen JA, Hunt JS (1999): Expression of cell adhesion
molecules in murine placentas and a placental cell line. Biol
Reprod, 60, 428-434.5. Burghardt RC, Bowen JA, Newton GR, et al. (1997):
Extracellular matrix and the implantation cascade in pigs.
J Reprod Fertil, 52,151-164.6. Butinar J, Mujagic E, Galac S. (2004): The oestrus cycle
in the bitch. Slov Vet Res, 41, 5-11.7. Chandra SA, Adler RR (2008): Frequency of different
estrous stages in purpose - bred beagles: A retrospective
study. Toxicol Pathol, 36, 944-949.8. Crossman GA (1937): A Modification of Mallory’s
connective tissue stain with a discussion of the principles
involved. Anat Rec, 69, 33-38.9. Fazleabas AT, Bell SC, Fleming S, et al. (1997):
Distribution of integrins and the extracellular matrix
proteins in the baboon endometrium during the menstrual
cycle and early pregnancy. Biol Reprod, 56, 348-356.10. Glasser SR, Lampelo S, Munir MI, et al. (1987):
Expression of desmin, laminin and fibronectin during in situ
differentiation (decidualization) of rat uterine stromal cells.
Differentiation, 35, 132-142.11. Kayışlı ÜA, Asar M, Demir R (2000): Ratlarda
desidualizasyon süresince ekstrasellüler matriksin yeniden
modellenmesinde laminin ve fibronektin ile reseptör alt
birimleri integrin β4 ve α5’in dağılımları ve muhtemel
rolleri. Turk J Biol, 24, 379 - 395.12. Kimmins S, Maclaren LA (1999): Cyclic modulation of
integrin expression in bovine endometrium. Biol Reprod,
61, 1267-1274.13. Klentzeris LD, Bulmer JN, Trejdosiewicz LK, et al.
(1993): Beta-1 integrin cell adhesion molecules in theendometrium of fertile and infertile women. Hum Reprod, 8,
1223-1230.14. Korgun ET, Çayli S, Asar M, et al. (2007): Distribution
of laminin, vimentin and desmin in the rat uterus during
initial stages of implantation. J Mol Hist, 38, 253-260.15. Lessey BA, Damjanovich L, Coutifaris C, et al. (1992):
Integrin adhesion molecules in the human endometriumcorrelation with the normal and abnormal menstrual cycle.
J Clin Invest, 90, 188-195.16. Lessey BA, Ilesanmi AO, Lessey MA, et al. (1996):
Luminal and glandular endometrial epithelium express
integrins differently throughout the menstrual cycle:
implications for implantation, contraception, and infertility.
Am J Reprod Immunol, 35, 195-204.17. Mansour GD, Henry M, Ferreira MR (2003):
Immunohistochemical study of equine endometrial
extracellular matrix during the oestrous cycle. J Comp Path,
129, 316-319.18. Nishida T, Murakami J, Otori T (1991): Expression of
fibronectin receptor (integrin) in the uterus of rats in
relation to the estrous cycle. Histochemistry, 96, 279-283.19. Ogawa H, Takahashi M, Takahashi H, et al. (2001):
Histochemical observations during uterine involution in
meishan pigs. J Reprod Develop, 47, 83-89.20. Okkens AC, Kooistra HS (2006): Anoestrus in the dog: A
fascinating story. Reprod Dom Anim, 41, 291-296.21. Rashev P, Georgieva R, Rees D (2005): Expression of
alpha 5 beta 1integrin and fibronectin during early
pregnancy in pigs. Folia Biol, 51, 121-125.22. Ruoslahti E (1991): Integrins. J Clin Invest, 87, 1-5.
23. Tabibzadeh S (1992): Patterns of expression of integrin
molecules in human endometrium throughout the menstrual
cycle. Hum Reprod, 7, 876-882.24. Taylor CV, Letarte M, Lye SJ (1996): The expression of
integrins and cadherins in normal human uterus and uterine
leiomyomas. Am J of Obstet Gynecol, 175, 411-419.25. Timpl R, Rohde H, Robey PG, et al. (1979): Laminin - a
glycoprotein from basement membranes. J Biol Chem, 254,
9933-9937.26. Van Cruchten S, Van den Broeck W, D’haeseleer M, et
al. (2004): Proliferation patterns in the canine endometrium
during the estrous cycle. Theriogenology, 62, 631-64130.27. Vermeirsch H, Simoens P, Hellemans A, et al. (2000):
Immunohistochemical detection of progesterone receptors
in the canine uterus and their relation to sex steroid
hormone levels. Theriogenology, 53, 773 -788.28. Wewer UM, Damjanov A, Weiss J, et al. (1986): Mouse
endometrial stromal cells produce basement-membrane
components. Differentiation, 32, 49-58.29. Yoshimura Y, Shiokawa S, Nagamatsu S, et al. (1995):
Effects of beta-1 integrins in the process of implantation.
Horm Res, 44, 36-41.30. Yoshimura Y (2002): The role of integrin in the human
reproductive process. J Reprod Develop, 48, 215-232.