ankara univ vet fak dergAnkara Üniversitesi Veteriner Fakültesi Dergisi1300-08611308-2817Ankara ÜniversitesiRatlarda siklofosfamidin sebep olduğu kardiyotoksisitede propolisin koruyucu rolüKayaEmreYılmazSevalÇolakoğluNeriman12312018661132010182017Çalışmada, kanser ve malignant olmayan hastalıkların tedavisinde etkili olduğu kanıtlanmış geniş klinik kullanımlıalkilleyici kemoterapötik bir ilaç olan siklofosfamid (CP)’in kardiyotoksisitesi üzerine propolisin etkileri incelenmiştir. Ratlar; kontrolgrubu, propolis uygulanan grup (200 mg/kg/gün gavaj, 7 gün), CP uygulanan grup (150 mg/kg i.p. tek doz) ve CP+propolis uygulanangrup şeklinde 4 gruba ayrılmıştır. Propolis uygulamasına CP uygulamasından 2 gün önce başlanmış ve 7 gün süre ile devam edilmiştir.Kalp dokuları biyokimyasal ve histopatolojik incelemeler için alınmıştır. Kalp dokusunda malondialdehid (MDA), redükte glutatyon(GSH) düzeyleri, katalaz (CAT), glutatyon peroksidaz (GSH-Px), glutatyon-S-transferaz (GST) ve süperoksit dismutaz (SOD)aktiviteleri tayini yapılmıştır. Histopatolojik inceleme için kalp dokusu örnekleri uygun yöntemlerle hazırlanıp Masson’nun Trichromeve Hematoksilen-Eozin tekniği ile boyandıktan sonra incelenmiştir. CP uygulanan grupta MDA, GSH düzeyleri ve CAT aktivitesindeartış, GSH-Px, GST ve SOD aktivitelerinde düşüş saptanmıştır (p<0.05). CP+propolis uygulanan grupta MDA, GSH düzeyleri veantioksidan enzim aktivitelerinin normal düzeylere ulaştığı gözlenmiştir. Propolis uygulanan grup ile CP+propolis uygulanan gruplardaistatistiksel olarak önemli bir değişiklik gözlenmemiştir. Histopatolojik incelemelerde kontrol grubunda kalp dokusuna ait kesitlernormal yapıda gözlenmiştir. Propolis uygulanan grupta kalp dokusu kontrol grubu ile benzer yapı göstermiştir. CP uygulanan gruptakalp kası lifleri arasında bazı alanlarda hemoraji ve vasküler konjesyon gözlenmiştir. CP+propolis uygulaması yer yer vaskülerkonjesyona yol açmıştır. CP’e bağlı kardiyotoksisitenin patogenezinde oksidatif stresin rol oynayabileceği, propolisin CP’e bağlı yanetkileri azaltabileceği görülmektedir. Antioksidan kalp malondialdehid propolis siklofosfamid1. Aebi H (1974): Catalase. 673-678. In: Bergmeyer HU (Ed).
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