ankara univ vet fak dergAnkara Üniversitesi Veteriner Fakültesi Dergisi1300-08611308-2817Ankara ÜniversitesiEffects of serum starvation and ionomycin activation on the development of somatic cell nuclear transfer embryos in sheepDemirKamberPabuccuoğluSerhatCiritÜmütEvecenMithatKaramanElifÖzdaşÖzen BanuAlkanSerhatAtallaHatemBirlerSema12312018661374211292017Synchronization of donor cells and activation of the reconstructed oocytes are important factors affecting the successrate in somatic cell cloning. In this study, it was aimed to investigate the effects of serum starvation in donor cell synchronization andionomycin treatment in the activation of reconstructed oocytes after somatic cell nuclear transfer in Kıvırcık sheep. Cumulus cells wereobtained from a slaughtered sheep ovaries and used as donor cells after serum starvation for 4 days (0.5% FCS; SS) or without serumstarvation (10% FCS; S). After reconstruction, oocytes were activated by ionomycin for 5 min plus 6-dimethylaminopurine for 3 h (I+)or only with 6-dimethylaminopurine for 3 h (I-). All cleaved embryos (n= 44) at the second day of in vitro culture were transferred intosynchronized recipient ewes (n= 10). Cleavage rates of the embryos were 37.3, 44.1, 34.6 and 44.7% in SS/I+, S/I+, SS/I- and S/Igroups, respectively. Recipient ewes had serum progesterone levels >1 ng/ml at 18th day were 33.3, 50.0, 50.0 and 100.0%, respectively.Only one pregnancy in the S/I- group continued after 40 days however the cloned lamb (7.1%, regarding to embryos transferred) died10 days before term due to a maternal problem (uterine torsion). The results of this study reveal that somatic cell synchronization byserum starvation and ionomycin treatment for the activation of oocytes can be omitted for the success of somatic cell nuclear transferin sheep Ionomycin serum starvation sheep somatic cell nuclear transfer1. Akagi S, Geshi M, Nagai T (2013): Recent Progress in
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