Öz
Introduction: The quantitative measurements are numerical data as the outputs of various processes. Statistical analysis is employed to the collected data. Results obtained from the statistical data are considered as more reliable. Therefore, measurement processes are the basis of research sciences today. Histology is a discipline that examines the microscopic structure of cells and tissues. Morphometric measurements can be performed on cell and tissue samples through electron and light microscope.
Materials and Methods: MEDLINE, SCIENCE DIRECT and Web of Science, Springer Link, Ovid, in the PubMed search engine were screened with the words “Testis, histopathology, quantitative, seminiferous tubule” between to find the studies.
Results: The findings obtained through these qualitative methods provide interpretation of the changes in tissue morphology. These assessments allow researchers to identify the tissue samples and to compare the physiological variations in its morphology. In histology, the results obtained from routine dyeing of laboratory studies are qualitative, therefore, relative differences may emerge in the interpretation of the results. In order to eliminate this risk, various quantitative measurement methods are implemented.
Discussion and Conclusion: Today, in order to employ accurate evaluations, the histometric and stereological measurement methods that are used in organs such as testicles, liver, lung, and kidney gain importance. The quantitative data obtained from these transactions is sufficient for statistical analysis. It is also important to reach a certain standardization level in the repetition of qualitative or semi-quantitative data obtained during the statistical analyses. The aim of this study is to summarize the methods used in histopathological evaluation of testicular tissues.
Anahtar Kelimeler
Kaynakça
- Abercrombie M, (1946) Estimation of nuclear population from microtome sections. Anat Rec, 94:239–247. PMID: 21015608
- Amann RP, Almquist JO, (1962) Reproductive capacity of dairy bulls.VIII. Direct and indirect measurement of testicular sperm production. J Dairy Sci, 45:774–781
- Aydıner Ç.A, Pul M, İnan M, Bilgi S, Çakır E, (2012) Can N-acetylcysteine play a role in preventing tissue damage in an experimental testicular torsion model? Cumhuriyet Journal of Medicine, 34: 462-471. Doi: 10.7197/1305-0028.1498
- Bozkurt M, (2018) Protective effect of hydrogen sulfide in experimental testicular ischemia reperfusion injury in rats. Health Sciences University Okmeydanı Health Application and Research Center, Urology Clinic, Thesis.
- Cosentino MJ, Nishida M, Rabinowitz R, Cockett A.T, (1986) Histopathology of prepubertal rat testes subjected to various durations of spermatic cord torsion. J Androl, 7: 23-31.
- Cruz-Orıve L.M & Weibel E.R, (1990) Recent stereological methods for cell biology: a brief survey. Am J Physiol, 258(4 Pt 1):L148-56. Doi: 10.1152/ajplung.1990.258.4.L148
- Eşrefoğlu M, (2016) General Histology, İstanbul Medicine Bookstore, İstanbul 2; 3p.
- Jafari O, Babaei H, Kheirandish R, Samimi A.S, Zahmatkesh A, (2018) Histomorphometric evaluation of mice testicular tissue following short- and long-term effects of lipopolysaccharideinduced endotoxemia. Iran J Basic Med Sci, Vol. 21, No. 1.
- Johnsen SG, (1970) Testicular biopsy score count- a method for registration of spermatogenesis in human normal values and results in 335 hypogonadal males. Hormones, 1:2-25.
- Kazemi S, Feizi F, Aghapour F, Joorsaraee G.A, Moghadamnia A.A, (2016) Histopathology and histomorphometric ınvestigation of bisphenol a and nonylphenol on the male rat reproductive system. North American Journal of Medical Sciences, 8(5): 215-221. Doi: 10.4103/1947-2714.183012
- Kianifard D, Sadrkhanlou R. A, Hasanzadeh S, (2012) The ultrastructural changes of the sertoli and leydig cells following streptozotocin induced diabetes. Iranian Journal of Basic Medical Sciences, 15(1), 623.
- Klopfleisch R, (2013) Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology - a systematic review. BMC Veterinary Research, 9:123. Doi: doi.org/10.1186/1746-6148-9-123
- Morais DB, Puga LCHP, Paula TAR., Freitas MBD, Matta SLP, (2017) The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view. Plos One, 1-18. https://doi.org/10.1371/journal.pone.0173856
- Özgüner M, Şenol A, Ural M, İşler M, (2004) Histological changes caused by experimental hyperthyroidism in adult rat testicular tissue. S.D.Ü. Medicine Facultary Journal, 11(3):1-6
- Renshaw A.A, Gould EW, (2007) Measuring errors in surgical pathology in real-life practice - Defining what does and does not matter. Am J Clin Pathol, 127(1):144–152
- Riber-Hansen R, Vainer B, Steiniche T (2012) Digital image analysis: a review of reproducibility, stability and basic requirements for optimal results. APMIS, 120(4):276–289.
- Şahin T.C, (2016) Protective effect of montelukast on torsion and detorsion injury in rats. Eskişehir Osmangazi University Institute of Health Sciences Department of Histology and Embryology, Master Thesis.
- Songur A, Karateke H, Tosun M, Gönül Y, Turamanlar O, (2016) Histomorphometric and immunohistochemical evaluation of testis tissue and blood testis barrier development in rats in postnatal period. Kocatepe Medicine Journal, 17(2).
- Yalçın T, (2018) The effect of oleuropein on apoptotic changes in testicular tissue in diabetic rats. Inonu University Journal of Health Sciences, 7 (2): 29-34. ISSN:2146-6696
Öz
Kantitatif
ölçümler, çeşitli süreçlerin çıktıları olan sayısal verilerdir. Toplanan
verilerin istatiksel analizleri
yapılmaktadır. Bu istatiksel verilerden elde edilen sonuçlar daha güvenilir
olarak kabul edilmektedir. Bu nedenle günümüzde ölçüm işlemleri araştırma
bilimlerinin temelini oluşturmaktadır. Histoloji, hücrelerin ve dokuların
mikroskopik yapısını inceleyen bilim dalıdır.
Testis, histopatoloji, kantitatif, seminifer tübül kelimelerini MEDLINE,
SCIENCE DIRECT, Web of Science, Springer Link, Ovid, ve PubMed arama motorları
kullanılarak literature taramaları yapıldı.Hücre ve doku örneklerinden elektron
ve ışık mikroskobu kullanılarak morfometrik ölçümler yapılabilmektedir. Bu
kalitatif yöntemlerle elde edilen bulgular doku morfolojisindeki
değişikliklerin yorumlanmasını sağlamaktadır. Bu değerlendirmeler
araştırmacıların doku örneklerini tanımasını ve morfolojisindeki fizyolojik
varyasyonların karşılaştırılmasını sağlamaktadır. Histolojide yapılan
laboratuvar çalışmalarında rutin boyamalarda elde edilen sonuçlar kalitatif
olduğundan, sonuçların yorumlanması aşamasında görece farklılıklar ortaya
çıkabilir. Bu riski ortadan kaldırmak için çeşitli kantitatif ölçüm yöntemleri
kullanılmaktadır. Günümüzde, doğru değerlendirme yapabilmek için testis,
karaciğer, akciğer, böbrek gibi organlarda kullanılan histometrik ve
stereolojik ölçüm yöntemleri önem arz etmektedir. Bu işlemler sonucu elde
edilen kantitatif veriler, istatistiksel analizler için yeterlidir. Ayrıca
istatiksel analizler yapılırken elde edilen kalitatif ya da semikantitatif
verilerin tekrarlanmasında belirli bir standardizasyonun yakalamasında önem arz
etmektedir. Bu çalışmanın amacı testis dokusunun histopatolojik değerlendirilmesinde
kullanılan yöntemleri özetlemektir.
Anahtar Kelimeler
Kaynakça
- Abercrombie M, (1946) Estimation of nuclear population from microtome sections. Anat Rec, 94:239–247. PMID: 21015608
- Amann RP, Almquist JO, (1962) Reproductive capacity of dairy bulls.VIII. Direct and indirect measurement of testicular sperm production. J Dairy Sci, 45:774–781
- Aydıner Ç.A, Pul M, İnan M, Bilgi S, Çakır E, (2012) Can N-acetylcysteine play a role in preventing tissue damage in an experimental testicular torsion model? Cumhuriyet Journal of Medicine, 34: 462-471. Doi: 10.7197/1305-0028.1498
- Bozkurt M, (2018) Protective effect of hydrogen sulfide in experimental testicular ischemia reperfusion injury in rats. Health Sciences University Okmeydanı Health Application and Research Center, Urology Clinic, Thesis.
- Cosentino MJ, Nishida M, Rabinowitz R, Cockett A.T, (1986) Histopathology of prepubertal rat testes subjected to various durations of spermatic cord torsion. J Androl, 7: 23-31.
- Cruz-Orıve L.M & Weibel E.R, (1990) Recent stereological methods for cell biology: a brief survey. Am J Physiol, 258(4 Pt 1):L148-56. Doi: 10.1152/ajplung.1990.258.4.L148
- Eşrefoğlu M, (2016) General Histology, İstanbul Medicine Bookstore, İstanbul 2; 3p.
- Jafari O, Babaei H, Kheirandish R, Samimi A.S, Zahmatkesh A, (2018) Histomorphometric evaluation of mice testicular tissue following short- and long-term effects of lipopolysaccharideinduced endotoxemia. Iran J Basic Med Sci, Vol. 21, No. 1.
- Johnsen SG, (1970) Testicular biopsy score count- a method for registration of spermatogenesis in human normal values and results in 335 hypogonadal males. Hormones, 1:2-25.
- Kazemi S, Feizi F, Aghapour F, Joorsaraee G.A, Moghadamnia A.A, (2016) Histopathology and histomorphometric ınvestigation of bisphenol a and nonylphenol on the male rat reproductive system. North American Journal of Medical Sciences, 8(5): 215-221. Doi: 10.4103/1947-2714.183012
- Kianifard D, Sadrkhanlou R. A, Hasanzadeh S, (2012) The ultrastructural changes of the sertoli and leydig cells following streptozotocin induced diabetes. Iranian Journal of Basic Medical Sciences, 15(1), 623.
- Klopfleisch R, (2013) Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology - a systematic review. BMC Veterinary Research, 9:123. Doi: doi.org/10.1186/1746-6148-9-123
- Morais DB, Puga LCHP, Paula TAR., Freitas MBD, Matta SLP, (2017) The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view. Plos One, 1-18. https://doi.org/10.1371/journal.pone.0173856
- Özgüner M, Şenol A, Ural M, İşler M, (2004) Histological changes caused by experimental hyperthyroidism in adult rat testicular tissue. S.D.Ü. Medicine Facultary Journal, 11(3):1-6
- Renshaw A.A, Gould EW, (2007) Measuring errors in surgical pathology in real-life practice - Defining what does and does not matter. Am J Clin Pathol, 127(1):144–152
- Riber-Hansen R, Vainer B, Steiniche T (2012) Digital image analysis: a review of reproducibility, stability and basic requirements for optimal results. APMIS, 120(4):276–289.
- Şahin T.C, (2016) Protective effect of montelukast on torsion and detorsion injury in rats. Eskişehir Osmangazi University Institute of Health Sciences Department of Histology and Embryology, Master Thesis.
- Songur A, Karateke H, Tosun M, Gönül Y, Turamanlar O, (2016) Histomorphometric and immunohistochemical evaluation of testis tissue and blood testis barrier development in rats in postnatal period. Kocatepe Medicine Journal, 17(2).
- Yalçın T, (2018) The effect of oleuropein on apoptotic changes in testicular tissue in diabetic rats. Inonu University Journal of Health Sciences, 7 (2): 29-34. ISSN:2146-6696
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Derleme Makale |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2020 |
| Gönderilme Tarihi | 11 Eylül 2019 |
| Kabul Tarihi | 7 Ocak 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 10 Sayı: 1 |
