Investigation of TNF-α and NF-κB Levels in Masseter Muscle of Rats with High Fructose Corn Syrup-induced Metabolic Syndrome

Ömer Ekici; Mehmet Bilgehan Pektaş

Araştırma Makalesi

Yıl 2023, Cilt: 2 Sayı: 4, 77 - 82, 25.12.2023

Ömer Ekici Mehmet Bilgehan Pektaş

Öz

Kaynakça

1. Alberti KGMM, Eckel RH, Grundy SM, et al. Harmonizing the metabolic syndrome: A joint interim statement of the international diabetes federation task force on epidemiology and prevention; National heart, lung, and blood institute; American heart association; World heart federation; International atherosclerosis society; And international association for the study of obesity. Circulation. 2009;120(16):1640-1645. doi:10.1161/ CIRCULATIONAHA.109.192644
2. Bray GA. Energy and fructose from beverages sweetened with sugar or high-fructose corn syrup pose a health risk for some people. Adv Nutr. 2013;4(2):220-225. doi:10.3945/ an.112.002816
3. Brown L, Panchal SK. Rodent models for metabolic syndrome research. J Biomed Biotechnol. 2011;2011. doi:10.1155/2011/351982
4. Felice JI, Gangoiti MV, Molinuevo MS, McCarthy AD, Cortizo AM. Effects of a metabolic syndrome induced by a fructose-rich diet on bone metabolism in rats. Metabolism. 2014;63(2):296-305. doi:10.1016/j.metabol.2013.11.002
5. Sánchez GA, Trinks PW, Richard SB, Di Croce DE, Takara D. Expression of sarcoplasmic-endoplasmic reticulum Ca- ATPase isoforms in masticatory muscles. Eur J Oral Sci. 2014;122(1):36-41. doi:10.1111/eos.12098
6. Li Q, Zhang M, Chen YJ, Wang YJ, Huang F, Liu J. Oxidative damage and HSP70 expression in masseter muscle induced by psychological stress in rats. Physiol Behav. 2011;104(3):365-372. doi:10.1016/j.physbeh.2011.04.012
7. Han DS, Yu CS, Chiang CK, et al. Serum myostatin is reduced in individuals with metabolic syndrome. PLoS One. 2014;9(9). doi:10.1371/journal.pone.0108230
8. Russell AP, Gastaldi G, Bobbioni-Harsch E, et al. Lipid peroxidation in skeletal muscle of obese as compared to endurance-trained humans: A case of good vs. bad lipids? FEBS Lett. 2003;551(1-3):104-106. doi:10.1016/S0014- 5793(03)00875-5
9. Reid MB, Shoji T, Moody MR, Entman ML. Reactive oxygen in skeletal muscle. II. Extracellular release of free radicals. J Appl Physiol. 1992;73(5):1805-1809. doi:10.1152/ jappl.1992.73.5.1805
10. Tükel HC, Alptekin Ö, Turan B, Delilbaşi E. Effects of metabolic syndrome on masseter muscle of male Wistar rats. Eur J Oral Sci. 2015;123(6):432-438. doi:10.1111/ eos.12226
11. Bray GA, Nielsen SJ, Popkin BM. Consumption of highfructose corn syrup in beverages may play a role in the epidemic of obesity. Am J Clin Nutr. 2004;79(4):537-543. doi:10.1093/ajcn/79.4.537
12. Babacanoglu C, Yildirim N, Sadi G, Pektas MB, Akar F. Resveratrol prevents high-fructose corn syrup-induced vascular insulin resistance and dysfunction in rats. Food Chem Toxicol. 2013;60:160-167. doi:10.1016/j. fct.2013.07.026
13. Ma X, Lin L, Yue J, et al. Suppression of ghrelin exacerbates HFCS-induced adiposity and insulin resistance. Int J Mol Sci. 2017;18(6). doi:10.3390/ijms18061302
14. Sadi G, Ergin V, Yilmaz G, et al. High-fructose corn syrupinduced hepatic dysfunction in rats: improving effect of resveratrol. Eur J Nutr. 2015;54(6):895-904. doi:10.1007/ s00394-014-0765-1
15. Ma X, Lin L, Yue J, et al. Ghrelin receptor regulates HFCSinduced adipose inflammation and insulin resistance. Nutr Diabetes. 2013;3(12):e99-e99. doi:10.1038/nutd.2013.41
16. Advanced glycation end-product pentosidine accumulates in various tissues of rats with high fructose intake - PubMed. https://pubmed.ncbi.nlm.nih.gov/17298207/. Accessed April 10, 2021.
17. Advanced glycation end products affect growth and function of osteoblasts - PubMed. https://pubmed.ncbi. nlm.nih.gov/21906430/. Accessed April 10, 2021.
18. Li Q, Zhang M, Chen YJ, Wang YJ, Huang F, Liu J. Oxidative damage and HSP70 expression in masseter muscle induced by psychological stress in rats. Physiol Behav. 2011;104(3):365-372. doi:10.1016/j.physbeh.2011.04.012
19. Aghabeigi B, Haque M, Wasil M, Hodges SJ, Henderson B, Harris M. The role of oxygen free radicals in idiopathic facial pain. Br J Oral Maxillofac Surg. 1997;35(3):161-165. doi:10.1016/S0266-4356(97)90555-6
20. Viggiano A, Monda M, Viggiano A, et al. Trigeminal pain transmission requires reactive oxygen species production. Brain Res. 2005;1050(1-2):72-78. doi:10.1016/j. brainres.2005.05.021
21. Ozgocmen S, Ozyurt H, Sogut S, Akyol O, Ardicoglu O, Yildizhan H. Antioxidant status, lipid peroxidation and nitric oxide in fibromyalgia: Etiologic and therapeutic concerns. Rheumatol Int. 2006;26(7):598-603. doi:10.1007/s00296- 005-0079-y
22. Etoz OA, Ataoglu H, Erel O, Celik H, Herken EN, Bayazit YA. Association of serum total antioxidant capacity and total oxidant status with pain perception in patients with myofacial pain dysfunction. Int J Neurosci. 2009;119(9):1282-1291. doi:10.1080/00207450902907482
23. Allen DG. Skeletal muscle function: Role of ionic changes in fatigue, damage and disease. Clin Exp Pharmacol Physiol. 2004;31(8):485-493. doi:10.1111/j.1440-1681.2004.04032.x
24. Kjeldsen K, Braendgaard H, Sidenius P, Larsen JS, Norgaard A. Diabetes decreases Na+-K+ pump concentration in skeletal muscles, heart ventricular muscle, and peripheral nerves of rat. Diabetes. 1987;36(7):842-848. doi:10.2337/ diab.36.7.842
25. Alterations in the masseter muscle and plasma IL-6 level following experimentally induced occlusal interference and chronic stress--a study in rats - PubMed. https://pubmed. ncbi.nlm.nih.gov/22856259/. Accessed June 30, 2021.

Investigation of TNF-α and NF-κB Levels in Masseter Muscle of Rats with High Fructose Corn Syrup-induced Metabolic Syndrome

Yıl 2023, Cilt: 2 Sayı: 4, 77 - 82, 25.12.2023

Ömer Ekici Mehmet Bilgehan Pektaş

Öz

Objective
In recent years, especially due to excessive sugar consumption, metabolic Syndrome (MetS) has become increasingly common
in the world and in Turkey. The aim of the study was to investigate the inflammatory effects of high fructose corn syrup (HFCS)
intake on the masseter muscle of young rats.
Materials and methods
Sixteen 3-week-old male Wistar rats were randomly split into two groups: control and HFCS. Animals were given HFCS in the
form of 20% solutions in drinking water. Animals were sacrificed in the eighth week. Right masseter muscle was isolated for
immunohistochemical and histopathological examination, and left masseter muscle was isolated for gene expression analysis.
Results
Both ELISA and real-time polymerase chain reaction (rt-PCR) measurements revealed that the HFCS group had significantly
higher TNF-a and NF-kB levels than the control group (p <0.05). Additionally, when comparing the HFCS group to the control
group, a higher degree of lymphocyte infiltration was seen.
Conclusion
According to study results, young rats’ masseter muscle tissue had significantly higher levels of TNF- and NF-B due to fructoseinduced
MetS. These findings suggest that MetS, through increased inflammation, can cause masseter muscle dysfunction,
injury, fatigue, and pain.

Anahtar Kelimeler

Metabolic syndrome, masseter muscle, inflammation, TNF-a, NF-κB.

Kaynakça

1. Alberti KGMM, Eckel RH, Grundy SM, et al. Harmonizing the metabolic syndrome: A joint interim statement of the international diabetes federation task force on epidemiology and prevention; National heart, lung, and blood institute; American heart association; World heart federation; International atherosclerosis society; And international association for the study of obesity. Circulation. 2009;120(16):1640-1645. doi:10.1161/ CIRCULATIONAHA.109.192644
2. Bray GA. Energy and fructose from beverages sweetened with sugar or high-fructose corn syrup pose a health risk for some people. Adv Nutr. 2013;4(2):220-225. doi:10.3945/ an.112.002816
3. Brown L, Panchal SK. Rodent models for metabolic syndrome research. J Biomed Biotechnol. 2011;2011. doi:10.1155/2011/351982
4. Felice JI, Gangoiti MV, Molinuevo MS, McCarthy AD, Cortizo AM. Effects of a metabolic syndrome induced by a fructose-rich diet on bone metabolism in rats. Metabolism. 2014;63(2):296-305. doi:10.1016/j.metabol.2013.11.002
5. Sánchez GA, Trinks PW, Richard SB, Di Croce DE, Takara D. Expression of sarcoplasmic-endoplasmic reticulum Ca- ATPase isoforms in masticatory muscles. Eur J Oral Sci. 2014;122(1):36-41. doi:10.1111/eos.12098
6. Li Q, Zhang M, Chen YJ, Wang YJ, Huang F, Liu J. Oxidative damage and HSP70 expression in masseter muscle induced by psychological stress in rats. Physiol Behav. 2011;104(3):365-372. doi:10.1016/j.physbeh.2011.04.012
7. Han DS, Yu CS, Chiang CK, et al. Serum myostatin is reduced in individuals with metabolic syndrome. PLoS One. 2014;9(9). doi:10.1371/journal.pone.0108230
8. Russell AP, Gastaldi G, Bobbioni-Harsch E, et al. Lipid peroxidation in skeletal muscle of obese as compared to endurance-trained humans: A case of good vs. bad lipids? FEBS Lett. 2003;551(1-3):104-106. doi:10.1016/S0014- 5793(03)00875-5
9. Reid MB, Shoji T, Moody MR, Entman ML. Reactive oxygen in skeletal muscle. II. Extracellular release of free radicals. J Appl Physiol. 1992;73(5):1805-1809. doi:10.1152/ jappl.1992.73.5.1805
10. Tükel HC, Alptekin Ö, Turan B, Delilbaşi E. Effects of metabolic syndrome on masseter muscle of male Wistar rats. Eur J Oral Sci. 2015;123(6):432-438. doi:10.1111/ eos.12226
11. Bray GA, Nielsen SJ, Popkin BM. Consumption of highfructose corn syrup in beverages may play a role in the epidemic of obesity. Am J Clin Nutr. 2004;79(4):537-543. doi:10.1093/ajcn/79.4.537
12. Babacanoglu C, Yildirim N, Sadi G, Pektas MB, Akar F. Resveratrol prevents high-fructose corn syrup-induced vascular insulin resistance and dysfunction in rats. Food Chem Toxicol. 2013;60:160-167. doi:10.1016/j. fct.2013.07.026
13. Ma X, Lin L, Yue J, et al. Suppression of ghrelin exacerbates HFCS-induced adiposity and insulin resistance. Int J Mol Sci. 2017;18(6). doi:10.3390/ijms18061302
14. Sadi G, Ergin V, Yilmaz G, et al. High-fructose corn syrupinduced hepatic dysfunction in rats: improving effect of resveratrol. Eur J Nutr. 2015;54(6):895-904. doi:10.1007/ s00394-014-0765-1
15. Ma X, Lin L, Yue J, et al. Ghrelin receptor regulates HFCSinduced adipose inflammation and insulin resistance. Nutr Diabetes. 2013;3(12):e99-e99. doi:10.1038/nutd.2013.41
16. Advanced glycation end-product pentosidine accumulates in various tissues of rats with high fructose intake - PubMed. https://pubmed.ncbi.nlm.nih.gov/17298207/. Accessed April 10, 2021.
17. Advanced glycation end products affect growth and function of osteoblasts - PubMed. https://pubmed.ncbi. nlm.nih.gov/21906430/. Accessed April 10, 2021.
18. Li Q, Zhang M, Chen YJ, Wang YJ, Huang F, Liu J. Oxidative damage and HSP70 expression in masseter muscle induced by psychological stress in rats. Physiol Behav. 2011;104(3):365-372. doi:10.1016/j.physbeh.2011.04.012
19. Aghabeigi B, Haque M, Wasil M, Hodges SJ, Henderson B, Harris M. The role of oxygen free radicals in idiopathic facial pain. Br J Oral Maxillofac Surg. 1997;35(3):161-165. doi:10.1016/S0266-4356(97)90555-6
20. Viggiano A, Monda M, Viggiano A, et al. Trigeminal pain transmission requires reactive oxygen species production. Brain Res. 2005;1050(1-2):72-78. doi:10.1016/j. brainres.2005.05.021
21. Ozgocmen S, Ozyurt H, Sogut S, Akyol O, Ardicoglu O, Yildizhan H. Antioxidant status, lipid peroxidation and nitric oxide in fibromyalgia: Etiologic and therapeutic concerns. Rheumatol Int. 2006;26(7):598-603. doi:10.1007/s00296- 005-0079-y
22. Etoz OA, Ataoglu H, Erel O, Celik H, Herken EN, Bayazit YA. Association of serum total antioxidant capacity and total oxidant status with pain perception in patients with myofacial pain dysfunction. Int J Neurosci. 2009;119(9):1282-1291. doi:10.1080/00207450902907482
23. Allen DG. Skeletal muscle function: Role of ionic changes in fatigue, damage and disease. Clin Exp Pharmacol Physiol. 2004;31(8):485-493. doi:10.1111/j.1440-1681.2004.04032.x
24. Kjeldsen K, Braendgaard H, Sidenius P, Larsen JS, Norgaard A. Diabetes decreases Na+-K+ pump concentration in skeletal muscles, heart ventricular muscle, and peripheral nerves of rat. Diabetes. 1987;36(7):842-848. doi:10.2337/ diab.36.7.842
25. Alterations in the masseter muscle and plasma IL-6 level following experimentally induced occlusal interference and chronic stress--a study in rats - PubMed. https://pubmed. ncbi.nlm.nih.gov/22856259/. Accessed June 30, 2021.

Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Ağız, Yüz ve Çene Cerrahisi
Bölüm	Araştırma Makalesi
Yazarlar	Ömer Ekici 0000-0002-7902-9601 Mehmet Bilgehan Pektaş 0000-0001-9790-8397
Erken Görünüm Tarihi	22 Aralık 2023
Yayımlanma Tarihi	25 Aralık 2023
Yayımlandığı Sayı	Yıl 2023 Cilt: 2 Sayı: 4

Kaynak Göster

Vancouver	Ekici Ö, Pektaş MB. Investigation of TNF-α and NF-κB Levels in Masseter Muscle of Rats with High Fructose Corn Syrup-induced Metabolic Syndrome. EJOMS. 2023;2(4):77-82.

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