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Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers

Yıl 2021, Cilt: 18 Sayı: 2, 84 - 88, 01.08.2021
https://doi.org/10.32707/ercivet.952884

Öz

The aim of the study, the relationship between coagulation parameters and embryonic loss in embryo transferred (ET) cows and heifers wereevaluated. The animal material of this study consisted of 19 cows and 19 heif-ers on farms located in East Mediterranean Agriculture Research Institute in Adana. Blood samples were collected before the ET application from the recipient cows and heifers. Coagulation parameters measured included prothrombin time (PT), activated thromboplastin time (APTT), fibrinogen, thrombin time (TT), anti-thrombin-III (AT3) and D-dimer using by Sysmex® CA-7000 and activated protein C resistance, protein C and protein S using by Sysmex CS-5100. The pregnancy rate was found 5/19 (26.3%) and 5/19 (26.3%) in cows and in heifers, respectively (P>0.05). The em-bryonic mortality ratio in cows was 60% (3/5) and in heifers it was 40% (2/5). The differences of D-dimer levels be-tween pregnant and non-pregnant animals were significant (P<0.05).The APTT levels between the groups with preg-nancy and embryonic loss were significantly different (P<0.05). As a result, it was determined that there is a relation-ship between plasma D-Dimer levels and embryonic loss in cows that were transferred embryo.To the best of authors’ knowledge, this is the first study reporting the relationship between coagulation parameters and embryonic loss in ET cows and heifers.

Kaynakça

  • Akyüz B, Sarıözkan S, Bayram D. Factor XI mutation in normally fertile and repeat breeding Holstein cows in the Middle Anatolian region of Turkey: A financial approach. Anim Prod Sci 2012; 52: 1042-45.
  • Alaçam E. Büyük ruminantlarda infertilite. Alaçam E. ed. In: Evcil Hayvanlarda Reprodüksiyon, Suni Tohumlama, Doğum ve İnfertilite. Konya: Dizgievi, 1994; ss. 265-89.
  • Arnljots B, Dahlbäck B. Protein S as an in vivo cofac-tor to activated protein C in prevention of microarte-rial thrombosis in rabbits. J Clin Invest 1995; 95(5): 1987-93.
  • Bick RL, Hoppensteadt D. Recurrent miscarriage syndrome and infertility due to blood coagulation protein/platelet defects: A review and update. Clin Appl Thrombosis/Hemostasis 2005; 11(1):1-13.
  • Diskin MG, Morris DG. Embryonic and early foetal losses in cattle and other ruminants. Reprod Domest Anim 2008; 43 (2): 260-7.
  • Diskin MG, Parr MH, Morris DG. Embryo death in cattle: An update. Reprod Fertil Dev 2011; 24 (1): 244-51.
  • Erez O, Novack L, Beer-Weisel R, Dukler D, Press F, Zlotnik A, Than NG, Tomer A, Mazor M. DIC score in pregnant women-a population based modifica-tion of the International Society on Thrombosis and Hemostasis Score. PLoS One 2014; 9(4); e93240.
  • Ergene O. İneklerde kromozomal, hormonal, beslen-me sorunlarına ve ısı stresine bağlı erken embri-yonik ölümler. Dicle Üniv Vet Fak Derg 2009; 2 (2): 36-41.
  • Gentry PA, Feldman BF, Liptrap RM. Haemostasis and parturition revisited: Comparative profiles in mammals. Comp Clin Path 1991; 1: 150-4.
  • Gökçe E, Irmak K. Dissemine intravasküler koagu-lasyon (DIC). Kafkas Üniv Vet Fak Derg 2007; 13 (2): 215-22.
  • Harvey JW. Differential diagnosis of bleeding disor-ders. The North American Veterinary Conference. January, 7-11, 2006; Orlando-Florida.
  • Herring J, McMichael M. Diagnostic approach to small animal bleeding disorders. Top Companion Anim Med 2012; 27 (2): 73-80.
  • Heuwieser W, Kautni J, Biesel M, Grunert E. Coagu-lation profile of dairy cattle in the periparturient peri-od. Zentralbl Veterinarmed A 1990a.; 37 (1): 8-15.
  • Heuwieser W, Kautni J, Grunert E. Coagulation pro-file in different stages of pregnancy and under con-sideration of placental expulsion in dairy cattle. Zentralbl Veterinarmed A 1990b; 37 (4): 310-5.
  • Ivanov P, Tsvyatkovska T, Konova E, Komsa-Penkova R. Inherited thrombophilia and IVF failure: the impact of coagulation disorders on implantation process. Am J Reprod Immunol 2012; 68: 189-98.
  • King WA. Embryo-mediated pregnancy failure in cat-tle. Can Vet J 1991; 32: 99-103.
  • Kujovich JL. Factor V Leiden thrombophilia. Genet Med 2011; 13 (1): 1-16.
  • McNeill RE, Diskin MG, Sreenan JM, Morris DG. As-sociations between milk progesterone concentra-tion on different days and with embryo survival during the early luteal phase in dairy cows. Theriogenology 2006; 65 (7): 1435-41.
  • Mukhopadhyaya PN, Jha M, Muraleedharan P, Gup-ta RR, Rathod RN, Mehta HH, Khoda VK. Simula-tion of normal, carrier and affected controls for large-scale genotyping of cattle for factor XI defi-ciency. Genet Mol Res 2006; 5 (2): 323-32.
  • Noyan T. Klinik tanı ve laboratuar pratiğinde D-dimer testi. Türk Klinik Biyokimya Derg 2012; 10 (1): 35-40.
  • O'Riordan MN, Higgins JR. Haemostasis in normal and abnormal pregnancy. Best Pract Res Clin Obstet Gynaecol 2003; 17 (3): 385-96.
  • Peters AR. Embryo mortality in the cow. Anim Breeding Abstracts 1996; 64: 587-98.
  • Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine, A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats. Tenth Edition. Phyladelphia: Saunders Ltd 2007.
  • Shalhub S, Dua A, Brooks J. Biomarkers in descend-ing thoracic aortic dissection. Semin Vasc Surg 2014; 27(3-4): 196-9.
  • Silke V, Diskin MG, Kenny DA, Boland MP, Dillon P, Mee JF, Sreenan JM. Extent, pattern and factors associated with late embryonic loss in dairy cows. Anim Reprod Sci 2002; 71(1-2): 1-12.
  • Simon A, Laufer N. Repeated implantation failure: Clinical approach. Fertility Steril 2012; 97(5): 1039-43.

Embriyo Transferi Yapılan İnek ve Düvelerde Koagulasyon Parametreleri ile Embriyonik Kayıp Arasındaki İlişkinin Araştırılması

Yıl 2021, Cilt: 18 Sayı: 2, 84 - 88, 01.08.2021
https://doi.org/10.32707/ercivet.952884

Öz

Çalışmanın amacı, embriyo transferi (ET) yapılan Holstein ırkı inek ve düvelerde, pıhtılaşma parametreleri ile embriyonik ölüm arasındaki ilişkinin araştırılmasıdır. Çalışmaya, Doğu Akdeniz Tarım Araştırmaları Enstitüsü (Adana) çiftliklerinde bulunan 19 inek ve 19 düve dahil edildi. Alıcı inek ve düvelerden ET yapıldığı gün transfer öncesinde kan örnekleri alındı. Protrombin zamanı (PZ), aktive edilmiş parsiyel tromboplastin zamanı (APTZ), fibrinogen, trombin za-manı (TZ), antitrombin III (AT3) ve D-dimer gibi koagulasyon parametreleri Sysmex® CA- 7000 ve aktive edilmiş protein C rezistansı, protein C ve protein S ise Sysmex CS-5100 kullanılarak ölçüldü. Çalışmada gebelik oranı ineklerde 5/19 (%26.3) ve düvelerde 5/19 (%26.3) olarak belirlendi. Embriyonik ölüm oranı ineklerde %60 (3/5), ise düvelerde %40 (2/5) olarak belirlendi (P>0.05). Gebe olan ve olmayan hayvanlar arasında D-dimer seviyeleri arasındaki fark önemli bulundu (P<0.05). Gebelik devam eden ve embriyonik kayıp belirlenen gruplar arasında APTT seviyeleri arasındaki fark önemli bulundu (P<0.05). Sonuç olarak, embriyo transferi yapılan sığırlarda plazma D-dimer seviyeleri ile embriyo-nik kayıp arasında ilişki olduğu belirlendi. Yazarların bilgisine göre sunulan çalışma embriyo transferi yapılan inek ve düvelerde koagulasyon parametrelerinin değerlendirildiği ilk çalışma olması açısından önemlidir.

Kaynakça

  • Akyüz B, Sarıözkan S, Bayram D. Factor XI mutation in normally fertile and repeat breeding Holstein cows in the Middle Anatolian region of Turkey: A financial approach. Anim Prod Sci 2012; 52: 1042-45.
  • Alaçam E. Büyük ruminantlarda infertilite. Alaçam E. ed. In: Evcil Hayvanlarda Reprodüksiyon, Suni Tohumlama, Doğum ve İnfertilite. Konya: Dizgievi, 1994; ss. 265-89.
  • Arnljots B, Dahlbäck B. Protein S as an in vivo cofac-tor to activated protein C in prevention of microarte-rial thrombosis in rabbits. J Clin Invest 1995; 95(5): 1987-93.
  • Bick RL, Hoppensteadt D. Recurrent miscarriage syndrome and infertility due to blood coagulation protein/platelet defects: A review and update. Clin Appl Thrombosis/Hemostasis 2005; 11(1):1-13.
  • Diskin MG, Morris DG. Embryonic and early foetal losses in cattle and other ruminants. Reprod Domest Anim 2008; 43 (2): 260-7.
  • Diskin MG, Parr MH, Morris DG. Embryo death in cattle: An update. Reprod Fertil Dev 2011; 24 (1): 244-51.
  • Erez O, Novack L, Beer-Weisel R, Dukler D, Press F, Zlotnik A, Than NG, Tomer A, Mazor M. DIC score in pregnant women-a population based modifica-tion of the International Society on Thrombosis and Hemostasis Score. PLoS One 2014; 9(4); e93240.
  • Ergene O. İneklerde kromozomal, hormonal, beslen-me sorunlarına ve ısı stresine bağlı erken embri-yonik ölümler. Dicle Üniv Vet Fak Derg 2009; 2 (2): 36-41.
  • Gentry PA, Feldman BF, Liptrap RM. Haemostasis and parturition revisited: Comparative profiles in mammals. Comp Clin Path 1991; 1: 150-4.
  • Gökçe E, Irmak K. Dissemine intravasküler koagu-lasyon (DIC). Kafkas Üniv Vet Fak Derg 2007; 13 (2): 215-22.
  • Harvey JW. Differential diagnosis of bleeding disor-ders. The North American Veterinary Conference. January, 7-11, 2006; Orlando-Florida.
  • Herring J, McMichael M. Diagnostic approach to small animal bleeding disorders. Top Companion Anim Med 2012; 27 (2): 73-80.
  • Heuwieser W, Kautni J, Biesel M, Grunert E. Coagu-lation profile of dairy cattle in the periparturient peri-od. Zentralbl Veterinarmed A 1990a.; 37 (1): 8-15.
  • Heuwieser W, Kautni J, Grunert E. Coagulation pro-file in different stages of pregnancy and under con-sideration of placental expulsion in dairy cattle. Zentralbl Veterinarmed A 1990b; 37 (4): 310-5.
  • Ivanov P, Tsvyatkovska T, Konova E, Komsa-Penkova R. Inherited thrombophilia and IVF failure: the impact of coagulation disorders on implantation process. Am J Reprod Immunol 2012; 68: 189-98.
  • King WA. Embryo-mediated pregnancy failure in cat-tle. Can Vet J 1991; 32: 99-103.
  • Kujovich JL. Factor V Leiden thrombophilia. Genet Med 2011; 13 (1): 1-16.
  • McNeill RE, Diskin MG, Sreenan JM, Morris DG. As-sociations between milk progesterone concentra-tion on different days and with embryo survival during the early luteal phase in dairy cows. Theriogenology 2006; 65 (7): 1435-41.
  • Mukhopadhyaya PN, Jha M, Muraleedharan P, Gup-ta RR, Rathod RN, Mehta HH, Khoda VK. Simula-tion of normal, carrier and affected controls for large-scale genotyping of cattle for factor XI defi-ciency. Genet Mol Res 2006; 5 (2): 323-32.
  • Noyan T. Klinik tanı ve laboratuar pratiğinde D-dimer testi. Türk Klinik Biyokimya Derg 2012; 10 (1): 35-40.
  • O'Riordan MN, Higgins JR. Haemostasis in normal and abnormal pregnancy. Best Pract Res Clin Obstet Gynaecol 2003; 17 (3): 385-96.
  • Peters AR. Embryo mortality in the cow. Anim Breeding Abstracts 1996; 64: 587-98.
  • Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine, A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats. Tenth Edition. Phyladelphia: Saunders Ltd 2007.
  • Shalhub S, Dua A, Brooks J. Biomarkers in descend-ing thoracic aortic dissection. Semin Vasc Surg 2014; 27(3-4): 196-9.
  • Silke V, Diskin MG, Kenny DA, Boland MP, Dillon P, Mee JF, Sreenan JM. Extent, pattern and factors associated with late embryonic loss in dairy cows. Anim Reprod Sci 2002; 71(1-2): 1-12.
  • Simon A, Laufer N. Repeated implantation failure: Clinical approach. Fertility Steril 2012; 97(5): 1039-43.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Öznur Aslan Bu kişi benim 0000-0001-5479-3737

Kutlay Gürbulak Bu kişi benim 0000-0002-1176-9881

Uğur Kara Bu kişi benim 0000-0002-7977-6826

Erkan Say 0000-0003-0131-5912

Esra Canooğlu Bu kişi benim 0000-0002-7881-9484

Murat Abay 0000-0003-2457-1919

Yayımlanma Tarihi 1 Ağustos 2021
Gönderilme Tarihi 18 Şubat 2021
Kabul Tarihi 26 Nisan 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 18 Sayı: 2

Kaynak Göster

APA Aslan, Ö., Gürbulak, K., Kara, U., Say, E., vd. (2021). Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 18(2), 84-88. https://doi.org/10.32707/ercivet.952884
AMA Aslan Ö, Gürbulak K, Kara U, Say E, Canooğlu E, Abay M. Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers. Erciyes Üniv Vet Fak Derg. Ağustos 2021;18(2):84-88. doi:10.32707/ercivet.952884
Chicago Aslan, Öznur, Kutlay Gürbulak, Uğur Kara, Erkan Say, Esra Canooğlu, ve Murat Abay. “Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 18, sy. 2 (Ağustos 2021): 84-88. https://doi.org/10.32707/ercivet.952884.
EndNote Aslan Ö, Gürbulak K, Kara U, Say E, Canooğlu E, Abay M (01 Ağustos 2021) Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 18 2 84–88.
IEEE Ö. Aslan, K. Gürbulak, U. Kara, E. Say, E. Canooğlu, ve M. Abay, “Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers”, Erciyes Üniv Vet Fak Derg, c. 18, sy. 2, ss. 84–88, 2021, doi: 10.32707/ercivet.952884.
ISNAD Aslan, Öznur vd. “Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 18/2 (Ağustos 2021), 84-88. https://doi.org/10.32707/ercivet.952884.
JAMA Aslan Ö, Gürbulak K, Kara U, Say E, Canooğlu E, Abay M. Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers. Erciyes Üniv Vet Fak Derg. 2021;18:84–88.
MLA Aslan, Öznur vd. “Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, c. 18, sy. 2, 2021, ss. 84-88, doi:10.32707/ercivet.952884.
Vancouver Aslan Ö, Gürbulak K, Kara U, Say E, Canooğlu E, Abay M. Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers. Erciyes Üniv Vet Fak Derg. 2021;18(2):84-8.